1.Multivariate analysis of the influential factors in multinutrient treatment for neonate with infection pneumonia
Journal of Chongqing Medical University 2003;0(06):-
Objective:To explore the influence of background data in multinutrient treatment for neonate with infection pneumonia. To study the relative risk factors in VA nutritional state. Methods:the orally vitamin A and vitamin E,zinc, selenite daily in 111 neonates in the randomized,double-blind, placebo-controlled,clinical Trial for 7 days treatment. We investigated and analyze the relevant biochemical,immune evidence, clinic data and background data, with multivariate stepwise regression analysis. Results:Mother’s age, parity,gestation age and birth weight play positive influence to clinical restoring. Neonate’s age and birth weight play a positive role to serum IgM in early stage of pneumonia,but mother’ s age is negativity. Neonate’s age is positivity in enhancing serum VA,and is negativity in advancing serum IgM. Mother’s age play a negative role to heighten serum RBP. Conclusion:Mother’s age,parity,gestation age and neonate’s age is important factors to influence the clinical treatment effect. Great age and many parity mother’s neonate,earlier period and large for date infant are more possible to VAD during sick period,and are more slow to recover. So they need the nutritional intervention all the better.
2.Comparison on cellular immunity against Mycobacterium tuberculosis after intragastric and subcutaneous immunization of BCG in mice
Journal of Chongqing Medical University 2003;0(05):-
0.05).There were statistical significant differences in the cellular immunity between the immunization and no-immunization group( P
3.ULTRASTRUCTURE AND BATCH CULTURE OF MAGNETOTACTIC BACTERIA STRAIN WD-1
Wei JIANG ; Yangbao WEI ;
Microbiology 1992;0(03):-
The ultrastructure of magnetotactic bacteria strain WD\|1 was reported.A thin sectioned profile of it clearly showed its cell wall,cell membrane,poly\|?\|hydroxybutyrates and magnetosomes.Meanwhile,the method of batch culture of strain WD\|1 was also reported,135mg/L dry cells could be gotten with this method.The energy profiles of X\|ray collected after electron excitation in SEM indicated that both dry cells and magnetosomes contained such mineral elements as P,S,Ca,Si,Al and Fe with different content.The iron content of its cells and magnetosomes was 3 07% and 84 57% respectively.
4.Effect of Electroacupuncture plus Early Rehabilitation Training on Function Recovery after Surgery for Cervical Spinal Cord Injury
Shanghai Journal of Acupuncture and Moxibustion 2015;(1):43-44
Objective To investigate the effect of electroacupuncture plus early rehabilitation training on the function recovery after surgery for cervical spinal cord injury. Methods Sixty patients with cervical spinal cord injury undergone anterior cervical decompression and internal fixation were randomized into a treatment group and a control group, 30 cases in each group. The treatment group was intervened by electroacupuncture plus rehabilitation training, while the control group was by rehabilitation training alone. The Frankel’s grading (FG), Barthel Index (BI), and Functional Independence Measure (FIM) were adopted for evaluation before and after intervention. Results The FG was not significantly changed after intervention in both groups (P>0.05). After intervention, there was no significant difference in comparing FG between the two groups (P>0.05). The BI and FIM scores were significantly changed after intervention in both groups (P<0.01). There were significant differences in comparing BI and FIM scores between the two groups (P<0.05). Conclusion Electroacupuncture plus early rehabilitation training can promote the function recovery after surgery for cervical spinal cord injury, and improve the quality of life.
5.Two methods applied to comprehensive evaluation of hospital operation management
Chinese Journal of Hospital Administration 2013;29(10):753-759
Objective Using principal components analysis and weighted TOPSIS method to make objective and accurate evaluation of hospital operation management quality.This aims at proving whether such methods are scientific and feasible for comprehensive evaluation for the quality of hospital operation management and providing the basis for hospital decision making.Methods 15 typical Hospital Performance Indicators were chosen from a cancer hospital during 2008-2012,which were subject to TOPSIS method and principal components analysis,weighted TOPSIS method for comprehensive evaluation.Results The results with principal components analysis and weighted TOPSIS method conform to the actual hospital conditions and prove the rising quality of operation management of the hospital.That is,the closer the date,the higher the ranking.Conclusion Principal components analysis and weighted TOPSIS method are proven flexible,practical,scientific and reliable and suitable for popularization and application in the quality evaluation of hospital operation management.
6.The status of pregnancy thyroid screening indicators reference range establishment and methodology research
Chinese Journal of Laboratory Medicine 2013;(1):10-13
There is a significance of pregnancy thyroid hormone reference ranges establishment,because the consequences of thyroid dysfunction during pregnancy are terrible.This review will focus on meaning of pregnancy thyroid hormone reference range,importance of TPO antibody testing and current advances of methodology.
7.Expression of Th1/Th2 cytokine and T lymphocyte subset in rat allogenic corneal transplantation with organcultured graft
Chinese Journal of Experimental Ophthalmology 2012;30(2):121-126
BackgroundAllogenic immunological rejection is still the common cause of keratoplasty failure.Organ culture is a good choice for gradually attenuating corneal antigeniciy.However,the complicated regulatory mechanism of such allogenic rejection with organ culture gratts is unclear until now.ObjectiveTo investigate the changes of several Thl/Th2 cytokines and T lymphocyte subsets in the rat corneal allogenic transplantation rejection with organ culture grafts.MethodsThirty-six SPF Wistar rats served as donors,and 72 SPF SD rats served as recipients in this study.Seventy-two SD rats were divided into two groups randomly.Organ culture and fresh grafts from Wistar rats were performed on 36 recipients SD rats,respectively.Six normal SD rats were served as the normal control.After transplantation,graft survival time was observed ; Aqueous humor levels of IL-2,IFN-γ,IL-4 and TNF-α were measured by enzyme linked immunosorbent assay.Immunohistochemistry was performed to examine CD25 expressions in grafts.Levels of TNF-αmRNA,IFN-γmRNA,IL-4mRNA and CD25mRNA in grafts were detected by using reverse transcription polymerase chain reagction.The levels of CD28subsets in peripheralblood were determined by Flow Cytometry.The use of experimental animals followed the Statement of ARVO.Results The mean rat graft survival time was longer in the organ culture grafts group( 13.78 d) than that with fresh gratts( 10.56 d)(t=14.945,P =0.000 ).The aqueous humor levels of IL-2,IFN-γ,IL-4 and TNF-α were higher in two allograft groups at day 6,day 13 and day 24 after transplantation than that in the normal control,which peaked at day 13,and the fresh grafts group had the highest level( F=324.891,416.416,240.661,364.533,P=0.000).At day 13,CD25 was weakly expressed in frozen section of two operation groups.The expression of TNF-α and IFN-γmRNA in organ culture grafts group was markedly less than in fresh grafts group at day 13 after surgeries (t =2.464,P =0.039;t=5.438,P=0.001 ),whereas there was no significient difference in IL-4 and CD25 mRNA levels between them (t=-0.782,P =0.457,t =0.712,P =0.497).The percentages of CD28 of the fresh graft group increased significantly and more weakly expressed in organ culture grafts group (P =0.016 ). Conclusions Th1/Th2 cytokines and CD28 lymphocyte subsets may play important roles during corneal allograft rejection with organ culture grafts.
8.Effects of hydralazine on ocular blood flow and laser-induced choroidal neovascularization
International Eye Science 2008;8(12):2359-2363
·AIM: To investigate the effect of hydralazine on choroidal blood flow in rabbits and laser-induced choroidal neovascularization (CNV) in rats and the effect of hydralazine on tube formation of human umbilical vein endothelial cells (HUVEC).used with raised intraocular pressure (IOP) of the left eye to 40mmHg.Hydralazine (10g/L) eye drops were instilled and ocular blood flow was measured with colored microspheres technique. Male Brown Norway rats were treated with Nd: YAG laser to break Bruch's membrane. Hydralazine (5, 10, 20g/L) eye drops or saline alone was instilled three times a day for 4 weeks after laser treatment. Fluorescein angiography (FA) and choroidal flat mount were used to measure the area of CNV. Tube formation of HUVEC was studied at different concen-trations of hydralazine.hydralazine eye drops enhanced the choroidal blood flow significantly at 30 and 60 minutes after drug instillation. After 4 week of drug treatment, 5, 10 and 20g/L hydralazine eye drops all reduced the CNV formation dramatically measured by fluorescein angiography and choroidal flat mount. When HUVEC was cultured on matrix gel for 48 hours, hydralazine at 3-30mg/L preven-ted the tube formation.vivo and HUVEC tube formation in vitro, and enhanced rabbits' choroidal blood flow after ischemia. It is hoped that hydralazine could be used to treat age-related ma-cular degeneration in the future.
9.Analysis on complications of phacoemulification in primary angle -closure glaucoma after trabeculectomy
International Eye Science 2014;(6):1068-1070
AIM: To discuss the complications and prevention of phacoemulification in primary angle-closure glaucoma after trabeculectomy.
METHODS:Retrospective analysis of 33 cases (37 eyes) phacoemulification in primary angle-closure glaucoma after trabeculectomy in our hospital between January 2008 and June 2012, followed up 12-33mo, intraoperative and postoperative complications were observed.
RESULTS: Compared with pre-operation, intraocular pressure hadn't increased in follow-up 6mo. Iris was injured in 5 eyes, corneal edema was in 14 eyes, anterior chamber inflammation was in 16 eyes, all symptoms were improved in 3-7d. And no case with posterior capsule rupture or vitreous loss.
CONCLUSION: Phacoemulification is an effective way for the treatment of cataract after angle-closure glaucoma trabeculectomy, careful preoperative examination, intraoperative prevention can reduce or avoid the occurrence of surgical complications.
10.Axial symptoms after cervical laminoplasty
Chinese Journal of Orthopaedics 2017;37(9):569-576
Axial symptom,also called axial pain or axial neck pain,is a common complication after posterior cervical spine surgery.The symptom can be neck pain,as well as rigid,tension and dullness.The incidence of axial syndrome is estimated between 5.2% and 61.5%.Often,the symptom does not disappear spontaneously.The quality of patients' life as well as the effectiveness of cervical laminoplasty is affected.It can be divided into early and late axial pain based on the onset time.The mechanism and reason are different,but both are difficult to subside.The incident of axial symptom is relatively high in conventional laminoplasty.However the modified one which preserve posterior ligament-muscular structure can reduce the incidence significantly.C2 spinal process as the attachment of cervical semispinalis,could reduce the incidence of kyphosis after operation.And C7 spinal process is an important structure in posterior ligament-muscular structure.Both should be preserved as much as possible in order to maintain intact attachment of muscles,which facilitates the patient to restore soft tissue tension by rehabilitation.Rigid fixation is another method of modification,it could avoid re-close of the open door,and reduce soft tissue injury.However,attention must be paid not to destroy facet joint to cause new axial symptom.Risk factors of axial symptom include past shoulder and neck pain and stiffness,others remain controversial.There is no correlation between loss of range of motion after surgery as well as change of cervical alignment and axial symptom.It is more likely to be a different result due to muscle atrophy.Although there are no golden standard of laminoplasty,doctors should follow these principles:preserve C2 and C7 spinal process when possible,rigid fixation to avoid reclose,reduce immobilization after operation,and encourage patient to start rehabilitation as early as possible to reduce incident of axial symptom.