Objective To evaluate the role of the fractional dilatation and curettage (D&C) in the diagnosis of atypical hyperplasia of endometrium. Methods The retrospective analysis was performed in 48 patients diagnosed as atypical hyperplasia of endometrium and treated in our hospital from Aug.2009 to Dec.2010. We compared the pathology results of D&C with hysterectomy specimens, and investigated the reasons for the difference. Results The six patients (12.5%) with mild atypical hyperplasia of endometrium still presented with significant pathological changes after D&C (χ2 = 5.90, P＜0.05), 11 cases (22.9%) in 14 patients(29.2%) with moderate atypical hyperplasia (χ2=0.49, P＞0.01), 18 cases (100%) in patents with serious atypical hyperplasia, among which even 8 patients (44.4%) were verified as endometrial cancer. Conclusions There exists pathological discrepancy between the fractional D&C and hysterectomy specimens. Patients with severer atypical hyperplasia may have a higher risk of developing endometrial cancer.

Fecal microbiota transplantation(FMT),as a special organ transplantation,can be used to reconstruct the intestinal flora.It has been concerned by clinical practice,but it has not become a routine treatment method.With the gradual deepening of the study of FMT,the refractory difficile fusiform bacillus infection,inflammatory bowel disease,irritable bowel syndrome,chronic fatigue comprehensive syndrome and some metabolic disorders related to intestinal diseases were obtained using.In recent years,there are some reports about the application of FMT in children's diseases.Combining with the relevant literature at home and abroad,this paper reviews the indications and the taboo of the application of FMT in children,the aim is to provide a reference for FMT to be better applied in pediatric clinical.

Objective: To study the anti-aging effect of polypeptides from Fructus Lycii(PFL) on D-galactose(D-gal) induced aging model mice and the possible mechanism. Methods: Sixty ICR mice were randomly divided nto normal control group, D-gal induced model group, PFL 200, 400 and 800 mg/(kg · d) groups and vitamin E(VitE) 100 mg/(kg · d) group. D-gal aging mouse model was established by cervicodorsal region subcutaneous injection with D-gal(10 mg/kg) once a day for five successive weeks. In the meantime, drugs were given by intragastric administration respectively n PFL and Vit E treatment groups. The effect of PFL on learning and memory ability of mice was observed. After 5 weeks, the superoxide dismutase(SOD) activity, malondialdehyde(MDA) content and telomerase activity in serum, heart, liver and brain tissues of mice were measured. Results: Compared with normal control group, for aging model mice, the weight increasement declined, the number of errors in step-down test increased, the SOD and tolemerase activities in serum heart, liver and brain tissues dropped, and the MDA content was raised, P<0.01. Compared with model group, for the mice in PFL and VitE treatment groups, the weight increasement rised(P<0.01), the error number in step-down test decreased(P<0.05), the SOD activity in serum, heart, liver and brain tissues enhanced, and the MDA content reduced (P<0.01). The telomerase activity in serum and heart of 400, 800 mg/(kg · d) PFL and VitE treatment groups also increased significantly than model group, while that in liver and brain did not change. Conclusion: PFL have anti-aging effect on D-gal induced aging mice, and the action mechanism is related to the increasement of SOD activity, the decreasement of MDA content in serum, heart, liver and brain of D-gal aging mice, and the increasement of telomerase activity in serum and heart.

Animals ; Epilepsy ; genetics ; Gene Expression Regulation ; drug effects ; Glial Fibrillary Acidic Protein ; genetics ; metabolism ; Hippocampus ; metabolism ; Male ; Rats ; Rats, Sprague-Dawley ; Scorpion Venoms ; pharmacology

Objective To investigate effect of combination of rosuvastatin and dual antiplatelet therapy on the serum CRP, cerebral vascular event recurrence rate and carotid artery plaque in cerebral infarction patients.Method 60 cerebral infarction patients were seleted and divided into the control group and the experiment group by different treatment(n=30).Two groups were treated by corresponding drugs.The serum levels of IMT, CRP, plaque area, plaque number and the cases of recurrent cerebral vascular events after 6 month were compared after treatment a month.Results Compared with the control group after treatment,the serum CRP were lower(P<0.05),the recurrence rate of cerebral vascular events were lower(P<0.05),the IMT value, patch area and the number of carotid plaques were lower(P<0.05).Conclusion Rosuvastatin and dual antiplatelet combination therapy has good clinical effect for cerebral infarction patients,and have the clinical guiding significance.

Objective To observe the effect of Shenfu injection on intestinal function in rats with sepsis. Methods Forty Sprague-Dawley (SD) rats were randomly divided into four groups: sham operation, sepsis model, low and high concentration Shenfu injection groups, each groupn = 10. The sepsis model was replicated by cecal ligation and puncture (CLP), while the rate in sham operation group just underwent abdominal incision without CLP. Ten minutes after CLP, the low and high dose Shenfu injection groups were given 5 mL/kg and 10 mL/kg Shenfu intravenous injection via a tail vein respectively. The rats in the model group were treated by intravenous injection of 10 mL/kg normal saline through a tail vein in 10 minutes after CLP. Twelve hours later, the rats were sacrificed. The levels of Ghrelin, Gastrin, tumor necrosis factor-α (TNF-α), high mobility group B1 protein (HMGB1), myeloperoxidase (MPO) and diamine oxidase (DAO) activity in serum were detected by enzyme linked immunosorbent assay (ELISA). The levels of protein of Ghrelin and gastrin receptor (GHSR) were detected by Western Blot. Under light microscope, the histopathological changes in intestinal mucosa were investigated, and Chiu score was determined, and the apoptosis index (AI) of intestinal mucosal epithelial cells was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL).Results Compared with sham operation group, in model group, the levels of Ghrelin and Gastrin in serum were significantly decreased [Ghrelin (ng/L): 121.23±3.53 vs. 146.28±5.43, Gastrin (ng/L): 81.78±3.27 vs 102.78±4.07], the serum levels of TNF-α and HMGB1 were markedly increased [TNF-α (mg/L): 93.71±3.66 vs. 11.69±1.44, HMGB1 (mg/L): 76.25±4.12 vs. 22.41±3.08], the DAO activity and protein expressions of Ghrelin and GHSR of intestinal tissue were obviously decreased [DAO (U/mL): 14.64 ±0.68 vs. 25.13±1.98, Ghrelin (grey value): 0.12±0.02 vs. 0.23±0.04, GHSR (grey value): 0.18±0.02 vs. 0.32±0.03], the MPO activity in intestinal tissue, Chiu score of intestinal mucosa and AI of ileum mucosal epithelial cells were remarkably increased [MPO (mg/L): 175.98±6.95 vs. 45.64±4.48, Chiu score: 3.90±0.52 vs. 0.30±0.30, AI: 29.31±1.65 vs. 5.45±1.35, allP < 0.01]. Compared with model group, in low and high Shenfu injection groups, the levels of Ghrelin in serum and protein expressions of Ghrelin and GHSR in intestinal tissues were significantly increased (P < 0.05 orP < 0.01), the activity of DAO of intestinal tissues, the Chiu score and AI were significantly decreased, the degrees of changes being more significant in high Shenfu injection group than those in low Shenfu injection group [Ghrelin (ng/L): 143.54±3.89 vs. 136.58±4.91, TNF-α (mg/L): 75.13±4.69 vs. 83.70±4.40, HMGB1 (mg/L): 57.47±4.53 vs. 65.41±4.63, protein expression of Ghrelin (grey value): 0.18±0.03 vs. 0.15±0.03, protein expression of GHSR (grey value): 0.28±0.03 vs. 0.23±0.03, MPO (mg/L): 154.05±5.75 vs. 162.64 ±5.73, DAO (kU/L): 19.70±1.51 vs. 16.67±0.92, Chiu score: 2.30±0.52 vs. 3.20±0.48, AI: 20.38±1.34 vs. 26.40±1.32, allP < 0.05]. The levels of serum Gastrin in low and high Shenfu injection group were higher than those in model group, but no statistically significant differences were found (83.59±3.24, 86.54±5.93 vs. 81.78±3.27, bothP > 0.05). Under light microscope, the pathological changes were seen as follows: destruction and obvious edema of intestinal mucosal villi, ulcer formation, significant perivascular hemorrhage, presence of neutrophil infiltration and fracture of basement membrane in model group, while in low and high Shenfu groups, the intestinal villi had little defect, focal necrosis, small amounts of hemorrhage and neutrophil infiltration. Conclusions Shenfu injection can significantly improve the abnormal expressions of serum Ghrelin, reduce the levels of serum TNF-α and HMGB1, lowered MPO activity and enhance DAO activity in intestinal tissue, alleviate pathological changes in ileum mucosa, and decrease AI of ileum mucosal epithelial cells in rats with sepsis. And the degree of therapeutic effect is proportional to the Shenfu injection dose.

BACKGROUND: Senility is the degenerative change of every tissue and organ in biological body,which is the result due to comprehensive actions of various pathological and physiological processes. Rhodiola is a kind of natural medical plant,acting on delaying organic senility,preventing and treating senile disorders,etc. But the definite mechanisms on those have been unknown yet.OBJECTIVE: To probe into the mechanism of rhodiola extract( nuodikang capsule) on anti-senility.DESIGN: Randomized,controlled experimental research based on the experimental animals.SETTING: Neurological internal department in a military hospital affiliated to a university of Chinese PLA.MATERIALS: The experiment was performed in Pathology Experiment Room of Fourth Military Medical University of Chinese PLA from March to August 2002. Sixty Kunming mice were randomized into normal control group,model group,3 dosage groups of 1,2,4 g/kg of nuodikang successively and Vitamin E(Vit. E) positive control group 0. 1 mg/kg,10 mice in each group.INTERVENTIONS: In the model,nuodikang groups and Vit. E positive control,physiological saline solution of D-galactose 30 g/Lwas applied in subcutaneous at the back of neck,150 mg/kg per day,continuously for 8weeks. Simultaneously,in nuodikang group and Vit. E positive control,the medicine was applied by gastric perfusion,once daily,continuously for 8weeks.MAIN OUTCOME MEASURES: Effects of nuodikang capsule on activity of lactate dehydrogenase(LDH),the content of lipidperoxides(LPO),the activity of superoxide dismutase(SOD) in the brain,the content of malondialdehyde (MDA) and ultrastructure of mitochondrion in hippocampal neurons.RESULTS: In nuodikang capsule groups of 1,2,4 g/kg,the significant differences(P ＜ 0.05) presented in LPO content in brain tissue[(30. 1±2.9),(27.8±3.1),(26.9±1.9) nmol/g] and MDA content [(110.2±13.5),(95.4±20.1),(90.2±16.5) nmol/g],1 compared with the model group[ (33.4 ± 2.2),(126.5 ± 17.2) nmol/g],indicating significant differences(P ＜ 0.05),and LDH activity[(1.74±0. 14),(1.92 ±0. 23),(2.04 ±0.21) NU/g] and SOD activity[ (119. 1 ±2.2),(121.3 ± 0.9),(126.4 ± 2.0) NU/g] were remarkably increased,compared with the model group[ (1.68 ±0. 19),(115.9 ±2. 1) NU/g],indicating significant differences( P ＜ 0.05) . It was indicated by electro-microscope that nuodikang capsule groups of 2,4 g/kg acted on obvious protection on degenerative change of mitochondrion in hippocampal neurons caused by galactose.CONCLUSION: Nuodikang capsule acts significantly on anti-senility,which is related to its anti-oxidative injury and protection on neurons.

Objective:to study and solve some difficult problems in identification of medicinal plants in Selaginellaceae, and research the phylogenetic relationships among them.Methods:All the spectra were collected by using a Fourier transform near-infrared spectrometer equipped with a fiber-optic probe and a InGaAs detector for reflectance measurements.All the samples were scanned from 9 000 cm-1 to 4 000 cm-1,and each sample spectrum was obtained as an automatic mean of 32 times.The samples were measured 5 times.The average spectra obtained were transformed to first-order derivative spectra,and the latter were computerized with OPUS/INDENT operation software to conduct cluster analysis.Results:According to the information obtained from spectrograms and dendrograms,two types of S.heterostachys and two types of S.delicatula were identified as the same species respectively,and the variable type of the latter should be treated as a variety.S.tamariscina and S.pulvinata have very close relationship,indicating it is reasonable that they were applied as the same medicinal materials Juanbai.There is close phylogenetic relationship between S.moellendorffii and S.delicatula.This is also accord with the fact that they were used as the same folk herbal medicine.Conclusion:It shows that NIRS can be conveniently and quickly used for authentication of the medicinal plant species,resolving some correlative difficult problems,and inducing certain relationships between interspecies.

Objective To investigate the effects of NVP-BEZ235 on proliferation and apoptosis of human cholangiocarcinoma(CCA) cell QBC939 in vitro and to reveal the antineoplastic mechanisms of NVP-BEZ235.Methods Human CCA cell line QBC939 was used in this study.Cell apoptosis by NVP-BEZ235 was analyzed using the flow cytometry.Cell growth inhibition by NVP-BEZ235 for 24h 48h 72h by MTT assay.The antineoplastic mechanisms of NVP-BEZ235 for 48h were assessed by western blotting for PARP、Bcl-2、Akt、p-Akt、c-FLIPL and Mcl-1 assay in QBC939 cell.Results NVP-BEZ235 treatment inhibited the proliferation and induced apoptosis of human CCA cell QBC939,which appeared time-dependent and concentration-dependent effects.NVP-BEZ235 reduced protein levels of Mcl-1、c-FLIPL and Bcl-2 and downregulate protein level of p-Akt significantly.Conclusion NVP-BEZ235 inhibited the phosphorylation of Akt.NVP-BEZ235 downregulated Bcl-2、c-FLIPL、Mcl-1 protein level via PI3K/AKT signaling pathway to induce apoptosis and inhibit the proliferation of human CCA cell QBC939.

Objective:To explore the solutions to enhance the professional skills and humanistic quality of neurosurgery in theprimary hospitals, and to improve the neurosurgery medical care level.Methods: a comprehensive analysis of the literature retrieval as well as information collected in practice the first hospital of Zhangjiakou in, extract the problems of neurosurgeon training in primary hospital, to carry out grading treatment requirements, explore the basic path to improve the clinical ability of primary hospital neurosurgeon, and through the establishment of standard clinical operating procedures and systems to ensure the quality of medical service.Results: from Wanfang database retrieved from 2006 to 2015 research journals in Chinese on improving grassroots doctors ability of 819 papers, bibliometric analysis shows that the trend is increasing year by year, in 2015 issued a number is 5.21 times in 2006. There are 28 papers including neurosurgery area, accounting for 3.42% of the total number of papers; the hot topics focus in the mode of training neurosurgery doctors in primary hospitals,transfer the technology of minimally invasive neurosurgery and spreadmodern neurosurgery theory and knowledge.Conclusion: to improve the ability of doctorsof primary hospitals should be combined with the characteristics of local diseasesand primary hospital neurosurgeon status, from making training plan, appropriate to establish the corresponding clinical standard, promote the essential neurosurgery technology, in order to ensure implementation of the tiered diagnosis and treatment .