Objective To evaluate the role of the fractional dilatation and curettage (D&C) in the diagnosis of atypical hyperplasia of endometrium. Methods The retrospective analysis was performed in 48 patients diagnosed as atypical hyperplasia of endometrium and treated in our hospital from Aug.2009 to Dec.2010. We compared the pathology results of D&C with hysterectomy specimens, and investigated the reasons for the difference. Results The six patients (12.5%) with mild atypical hyperplasia of endometrium still presented with significant pathological changes after D&C (χ2 = 5.90, P＜0.05), 11 cases (22.9%) in 14 patients(29.2%) with moderate atypical hyperplasia (χ2=0.49, P＞0.01), 18 cases (100%) in patents with serious atypical hyperplasia, among which even 8 patients (44.4%) were verified as endometrial cancer. Conclusions There exists pathological discrepancy between the fractional D&C and hysterectomy specimens. Patients with severer atypical hyperplasia may have a higher risk of developing endometrial cancer.

Fecal microbiota transplantation(FMT),as a special organ transplantation,can be used to reconstruct the intestinal flora.It has been concerned by clinical practice,but it has not become a routine treatment method.With the gradual deepening of the study of FMT,the refractory difficile fusiform bacillus infection,inflammatory bowel disease,irritable bowel syndrome,chronic fatigue comprehensive syndrome and some metabolic disorders related to intestinal diseases were obtained using.In recent years,there are some reports about the application of FMT in children's diseases.Combining with the relevant literature at home and abroad,this paper reviews the indications and the taboo of the application of FMT in children,the aim is to provide a reference for FMT to be better applied in pediatric clinical.

Objective: To study the anti-aging effect of polypeptides from Fructus Lycii(PFL) on D-galactose(D-gal) induced aging model mice and the possible mechanism. Methods: Sixty ICR mice were randomly divided nto normal control group, D-gal induced model group, PFL 200, 400 and 800 mg/(kg · d) groups and vitamin E(VitE) 100 mg/(kg · d) group. D-gal aging mouse model was established by cervicodorsal region subcutaneous injection with D-gal(10 mg/kg) once a day for five successive weeks. In the meantime, drugs were given by intragastric administration respectively n PFL and Vit E treatment groups. The effect of PFL on learning and memory ability of mice was observed. After 5 weeks, the superoxide dismutase(SOD) activity, malondialdehyde(MDA) content and telomerase activity in serum, heart, liver and brain tissues of mice were measured. Results: Compared with normal control group, for aging model mice, the weight increasement declined, the number of errors in step-down test increased, the SOD and tolemerase activities in serum heart, liver and brain tissues dropped, and the MDA content was raised, P<0.01. Compared with model group, for the mice in PFL and VitE treatment groups, the weight increasement rised(P<0.01), the error number in step-down test decreased(P<0.05), the SOD activity in serum, heart, liver and brain tissues enhanced, and the MDA content reduced (P<0.01). The telomerase activity in serum and heart of 400, 800 mg/(kg · d) PFL and VitE treatment groups also increased significantly than model group, while that in liver and brain did not change. Conclusion: PFL have anti-aging effect on D-gal induced aging mice, and the action mechanism is related to the increasement of SOD activity, the decreasement of MDA content in serum, heart, liver and brain of D-gal aging mice, and the increasement of telomerase activity in serum and heart.

BACKGROUND: Senility is the degenerative change of every tissue and organ in biological body,which is the result due to comprehensive actions of various pathological and physiological processes. Rhodiola is a kind of natural medical plant,acting on delaying organic senility,preventing and treating senile disorders,etc. But the definite mechanisms on those have been unknown yet.OBJECTIVE: To probe into the mechanism of rhodiola extract( nuodikang capsule) on anti-senility.DESIGN: Randomized,controlled experimental research based on the experimental animals.SETTING: Neurological internal department in a military hospital affiliated to a university of Chinese PLA.MATERIALS: The experiment was performed in Pathology Experiment Room of Fourth Military Medical University of Chinese PLA from March to August 2002. Sixty Kunming mice were randomized into normal control group,model group,3 dosage groups of 1,2,4 g/kg of nuodikang successively and Vitamin E(Vit. E) positive control group 0. 1 mg/kg,10 mice in each group.INTERVENTIONS: In the model,nuodikang groups and Vit. E positive control,physiological saline solution of D-galactose 30 g/Lwas applied in subcutaneous at the back of neck,150 mg/kg per day,continuously for 8weeks. Simultaneously,in nuodikang group and Vit. E positive control,the medicine was applied by gastric perfusion,once daily,continuously for 8weeks.MAIN OUTCOME MEASURES: Effects of nuodikang capsule on activity of lactate dehydrogenase(LDH),the content of lipidperoxides(LPO),the activity of superoxide dismutase(SOD) in the brain,the content of malondialdehyde (MDA) and ultrastructure of mitochondrion in hippocampal neurons.RESULTS: In nuodikang capsule groups of 1,2,4 g/kg,the significant differences(P ＜ 0.05) presented in LPO content in brain tissue[(30. 1±2.9),(27.8±3.1),(26.9±1.9) nmol/g] and MDA content [(110.2±13.5),(95.4±20.1),(90.2±16.5) nmol/g],1 compared with the model group[ (33.4 ± 2.2),(126.5 ± 17.2) nmol/g],indicating significant differences(P ＜ 0.05),and LDH activity[(1.74±0. 14),(1.92 ±0. 23),(2.04 ±0.21) NU/g] and SOD activity[ (119. 1 ±2.2),(121.3 ± 0.9),(126.4 ± 2.0) NU/g] were remarkably increased,compared with the model group[ (1.68 ±0. 19),(115.9 ±2. 1) NU/g],indicating significant differences( P ＜ 0.05) . It was indicated by electro-microscope that nuodikang capsule groups of 2,4 g/kg acted on obvious protection on degenerative change of mitochondrion in hippocampal neurons caused by galactose.CONCLUSION: Nuodikang capsule acts significantly on anti-senility,which is related to its anti-oxidative injury and protection on neurons.

Objective To investigate the effects of NVP-BEZ235 on proliferation and apoptosis of human cholangiocarcinoma(CCA) cell QBC939 in vitro and to reveal the antineoplastic mechanisms of NVP-BEZ235.Methods Human CCA cell line QBC939 was used in this study.Cell apoptosis by NVP-BEZ235 was analyzed using the flow cytometry.Cell growth inhibition by NVP-BEZ235 for 24h 48h 72h by MTT assay.The antineoplastic mechanisms of NVP-BEZ235 for 48h were assessed by western blotting for PARP、Bcl-2、Akt、p-Akt、c-FLIPL and Mcl-1 assay in QBC939 cell.Results NVP-BEZ235 treatment inhibited the proliferation and induced apoptosis of human CCA cell QBC939,which appeared time-dependent and concentration-dependent effects.NVP-BEZ235 reduced protein levels of Mcl-1、c-FLIPL and Bcl-2 and downregulate protein level of p-Akt significantly.Conclusion NVP-BEZ235 inhibited the phosphorylation of Akt.NVP-BEZ235 downregulated Bcl-2、c-FLIPL、Mcl-1 protein level via PI3K/AKT signaling pathway to induce apoptosis and inhibit the proliferation of human CCA cell QBC939.

Objective: To summarize and analyze the early outcomes and clinical experience in 1198 patients undergone off-pump coronary artery bypass (OPCAB). Methods: This multi-center report covers 15 different institutes in China from October 1996 to May 2002. The ratio of OPCAB to conventional coronary artery bypass grafting (cCABG) was 85.2%. The mean age was (61.6?9.0) years. 77.3% patients were male and 22.7% were female. There were 34.6% patients with myocardial infarction, 58.2% with unstable angina pectoris, 18.7% with left main lesion, 76.9% with triple-vessel disease, 1.5% was redo surgery, and 9.9% emergent or urgent surgery. The mean left ventricular ejection fraction was 0.56?0.17. Results: The number of distal anastomosis was 3.1?0.9. IMA was used in 65.6% patients and radial artery was used in 18.0%. 13.5% OPCAB was combined with TMLR. IABP was used in 5.2% patients. The hospital mortality was 1.2%. The morbidity included 0.4% peri-operative myocardial infarction, 0.3% heart failure, 1.2% fatal arrhythmia, 2.0% pulmonary complications, 1.2% neurotic disorder, 0.7% renal dysfunction, 1.0% reopen for bleeding, and 0.6% delayed wound healing. Conclusion: OPCAB surgery is a safe and feasible procedure with good early results. Its late outcomes need further follow-up. OPCAB requires certain special skills and methods in practice so a specific training course should be designed.

Objective:to study and solve some difficult problems in identification of medicinal plants in Selaginellaceae, and research the phylogenetic relationships among them.Methods:All the spectra were collected by using a Fourier transform near-infrared spectrometer equipped with a fiber-optic probe and a InGaAs detector for reflectance measurements.All the samples were scanned from 9 000 cm-1 to 4 000 cm-1,and each sample spectrum was obtained as an automatic mean of 32 times.The samples were measured 5 times.The average spectra obtained were transformed to first-order derivative spectra,and the latter were computerized with OPUS/INDENT operation software to conduct cluster analysis.Results:According to the information obtained from spectrograms and dendrograms,two types of S.heterostachys and two types of S.delicatula were identified as the same species respectively,and the variable type of the latter should be treated as a variety.S.tamariscina and S.pulvinata have very close relationship,indicating it is reasonable that they were applied as the same medicinal materials Juanbai.There is close phylogenetic relationship between S.moellendorffii and S.delicatula.This is also accord with the fact that they were used as the same folk herbal medicine.Conclusion:It shows that NIRS can be conveniently and quickly used for authentication of the medicinal plant species,resolving some correlative difficult problems,and inducing certain relationships between interspecies.

To investigate the protective effects of OLA-PENa on experimental hepatic injury in rats and compare it with OLA in the dose-response relationships. METHODS: Biochemical indexes and histopathological examination of hepatic injury in rats caused by toxicant chemicals ［D-galactosamine (D-Galn) and CCl4］ were determined. RESULTS: OLA-PENa obviously inhibited the rising of serum alanine transaminase (ALT) caused by D-Galn and CCl4 and dramatically decreased liver fat storage as well in a dose-dependent manner. Histopathological examination showed that OLA-PENa can evidently alleviate the condition of the degeneration of hepatic cells and that of necrosis. CONCLUSION: The protective effects of OLA-PENa on experimental liver injury in rats, all the mentioned effects, are more powerful than those of OLA with the same dosage.

Objective To investigate the prevention and treatment for complications of chronic subdural hematoma using intracranial puncture and drainage. Methods Clinical data of 210 cases of chronic subdural hematoma treated by puncture and drainage were studied retrospectively. Results The cure rate was 97 1% (204/210), the recurrence rate was 7 1% (15/210), the incidence of complications was 14 3% (30/210), and the mortality, 0 95% (2/210). Conclusions Intracranial puncture and drainage for chronic subdural hematoma is safe, simple and effective. Prompt and proper treatment for complications encountered is required to obtain an excellent therapeutic effect.

Objective To study the value of insulin like growth factor binding protein-1 of cervicovaginal secretion in the delayed pregnancy termination. Methds Phosphorylated isoforms of IGFBP-1 in cervicovaginal secretion of 202 delayed pregnant women were measured by immmmunochromatography. Results In the IGFBP-1 positive group. The rate of successful pregnant termination in three days was 91 2%, while in IGFBP-1 negative group, the rate of that was 41 8%..There was statistically significant diference in the rate of successful pregnant termination between the two groups(P