Objective To investigate the effects of different dose of edible liquor on cardiac structure and function in rats ,and explore its underlying mechanisms .Methods 54 male SD rats were randomly divided into saline group ,alcohol group ,liquor group , each group were further divided into low-,middle-and high-dose groups .After 12 weeks of feeding ,left ventricular pressure was de-termined ,and left ventricular pressure amplitude and indexes of HW /BW were calculated .Myocardial fibrosis was observed by Masson staining ,AngⅡ content of myocardial tissue were detected by ELISA ,and ACE and ACE2 mRNA expressions were detec-ted by RT-PCR ,respectively .Results Left ventricular pressure amplitude and HW/BW index were increased by both liquor and al-cohol .The myocardial cell turbidity ,degeneration ,hyperplasia of fibrous tissue change were gradually worsened ,and the AngⅡ con-tents and the ACEmRNA expressions of the myocardial tissue were also increased by both liquor and alcohol .The ACE2 mRNA ex-pression of the myocardial tissue were higher by low-dose liquor and alcohol ,while the ACE2 mRNA expression of the myocardial tissue were lower by middle-and high-dose liquor and alcohol .Conclusion Long-term alcohol consumption can damage the balance of cardiac ACE and ACE2 ,causing the increase of ACE ,AngⅡ and the decrease of ACE2 ,resulting in myocardial fibrosis ,whose degree is related to the amount of alcohol consumption and drinking history .

By using Person's separating blood ghost method basically, Na~+-K~+ ATP ase and Ca~(2+)-Mg~(2+) ATPase activties of human erythrocyte membrane were studied. The comparison was made between 11 G6PD deficient subjects and 11 healthy control persons. The results showed that both enzyme activities as well as ouabain inhibition rate were decreased in G6PD deficient individuals (P

AIM: The multiple effective components in Yiqing Capsule(Radix et Rhizoma Rhei,Rhizoma Coptidis,Radix Scutellariae) were analyzed by RP-HPLC. METHODS: A column of Kromasil C_(18)(250 mm?4.6 mm,5 ?m)was used.The mobile phase was composed of A 0.5% triethylamine(pH3.0,containing 5 mmol/L SDS),B methanol and acetonitrile(50∶50) with gradient elution.The flow rate was 1.0 mL/min with the detection wavelength at 254 nm. RESULTS: In the linear range the correlation coefficients ranged from 0.999 4 to(0.999 9).The average recoveries were 95.2%-102.2% with corresponding RSD of 0.8%-2.0%,respectively. CONCLUSION: The method is rapid,accurate and reliable.It can be used to control the quality of Yiqing Capsule.

Background Fractalkine(FKN) is a novel chemokine that mediate the adhesion of monocyte to vascular endothelial cell,involving in the development of atherosclerosis(AS).Objective To investigate the effect of interleukin-18(IL-18) on the fractalkine expression in human umbilical vascular endothelial cells(HUVEC),and the role of fractalkine in the monocytes THP-1 adhesion on HUVEC.Methods Fractalkine expression in HUVEC was determined by reverse transcription-polymerase chain reaction(RT-PCR),and the adhesion of THP-1 to HUVEC was assessed by Flow Chamber.Results Incubation of HUVEC with IL-18 upregulated FKN mRNA expression in dose-dependent manner.In the concentration of IL-18 ranged 0,25,50,100 ?g/L,the FKN mRNA was increased from(0.10?0.01),(0.49?0.04),(0.63?0.09) to(0.85?0.07)(P trend

Axial symptom,also called axial pain or axial neck pain,is a common complication after posterior cervical spine surgery.The symptom can be neck pain,as well as rigid,tension and dullness.The incidence of axial syndrome is estimated between 5.2％ and 61.5％.Often,the symptom does not disappear spontaneously.The quality of patients' life as well as the effectiveness of cervical laminoplasty is affected.It can be divided into early and late axial pain based on the onset time.The mechanism and reason are different,but both are difficult to subside.The incident of axial symptom is relatively high in conventional laminoplasty.However the modified one which preserve posterior ligament-muscular structure can reduce the incidence significantly.C2 spinal process as the attachment of cervical semispinalis,could reduce the incidence of kyphosis after operation.And C7 spinal process is an important structure in posterior ligament-muscular structure.Both should be preserved as much as possible in order to maintain intact attachment of muscles,which facilitates the patient to restore soft tissue tension by rehabilitation.Rigid fixation is another method of modification,it could avoid re-close of the open door,and reduce soft tissue injury.However,attention must be paid not to destroy facet joint to cause new axial symptom.Risk factors of axial symptom include past shoulder and neck pain and stiffness,others remain controversial.There is no correlation between loss of range of motion after surgery as well as change of cervical alignment and axial symptom.It is more likely to be a different result due to muscle atrophy.Although there are no golden standard of laminoplasty,doctors should follow these principles:preserve C2 and C7 spinal process when possible,rigid fixation to avoid reclose,reduce immobilization after operation,and encourage patient to start rehabilitation as early as possible to reduce incident of axial symptom.

Objective]Collecting and studying Wang Fu and his book YiLing CuanYao TanYuan systematically. [Method]Literature research on Wang Fu's life and the versions ofYiLing CuanYao TanYuan, reviewing his concept of study and academic thoughts. [Result]Confucian Wang Fu wrote more than 30 manuscripts, his medical masterpiece“YiLing CuanYao TanYuan, existing versions of two Qing printed editions and one Qing transcript, guided by medical source, centered on traditional Chinese medicine and prescription, accounted for human disease, made a comprehensive and profound elaboration on the basic theory of TCM.The recipes and traditional Chinese medicine of this book have clinical significance . [Conclusion]The collecting and studying have important literature value. These are worthy of our future generations to inherit and carry forward.

Erectile dysfunction (ED) results from the interaction of many pathological factors. Studies show that a high incidence of ED is associated with chronic diseases of various systems, and its pathogenesis is not fully understood. This article outlines the progress in recent studies on the impact of chronic diseases on erectile function.
Chronic Disease ; Erectile Dysfunction ; etiology ; Humans ; Male ; Penile Erection ; physiology

Objective To detect expression of PRKACB protein in non-small cell lung cancer ,thus to study its clinical significance and to analyze the relationship between PRKACB expression and prognosis .Methods We detected the PRKACB protein using immunohistochemistry in 109 lung cancer tissues and corresponding adjacent 20 normal tissues of non-small cell lung cancer patients .Results Compared with normal tissues , the level of PRKACB significantly decreased in lung cancers (χ2 =25.217,P<0.05),and was associated with differentiation (χ2 =9.664,P<0.05),lymph node metastasis(χ2 =4.430,P<0.05),and TNM staging(χ2 =4.430,P<0.05). Furthermore,the low expression of PRKACB protein was correlated with poor prognosis ( overall survival and disease-free survival ) of the patients .Conclusion PRKACB was lowly expressed in non-small cell lung cancer ,and associat-ed with clinical features ,which indicated its important role in clinical diagnosis of non-small cell lung cancer .

Chronic pain is a common clinical disease,which brings great burden to the patients.However,the pathogenesis underlying of chronic pain is complicated,which is affected by many factors,such as physiology,psychology and society.Therefore,the treatment of chronic pain has been a problem in clinical practice.Considering its complexity,a single way of treatment usually could not reach satisfactory results,so combination therapy is often used to treat chronic pain at present.The combination therapy includes pharmacological treatment,psychological approaches,interventional treatment,self management and so on.The treatment plans are distinct for different types of chronic pain,even the individual patients with the same kind of pain.The emergence of interdisciplinary rehabilitation programs shed light upon the treatment of chronic pain recent years.This paper reviewed the research on chronic pain treatment,in order to provide theoretical basis for clinical practice.

Objective To study the protective role against cisplatin-induced ototoxicity by using radix astra-gali in rats. Methods Forty rats were randomly divided into four groups. Group A received saline as controls. Group B received radix astragali. Group C received injection of cisplatin(4 mg/kg) as experiments for 6 days. Group D received both radix astragali (5 g/kg) and cisplatin (4 mg/kg). Distortion product acoustic emission (DPOAE) was applied to each rat before and 7 days after cisplatin injection. All the animals were sacrificed on the 7th day. Half of the cochleas were observed by frozen section and the apoptosis of hair cells was detected by TUNEL meth-od. Scanning electron microscopy was utilized to evaluate the cochlear morphology of the other rats. Results The DPOAE amplitudes of Group C decreased significantly compared to the group A and group B(P<0.01). The dam-age and apoptosis of hair cells were noted in the group C and group D, while the hair cells of group A and group B showed no sign of apoptosis or damage. Compare to experimental group , the DPOAE amplitudes of group D were higher, and the damage and apoptosis of hair cells were significantly lower(P<0.05). Conclusion This study sug-gests that radix astragali can effectively reduce cisp[atin ototoxicity.