AIM:To establish an assay of oxymatrine and matrine in Compound Shiwei Tablets(Folium Pyrrosiae, Radix Sophorae Flavescentis, Radix Astragali, Herba Polygoni Avicularis, etc.) and Radix Sophorae Flavescentis by HPLC. METHODS: Diamonsil C 18 (10 ?m,250 mm?4.6 mm) analytical column was used.The mobile phase consisted of methanol-0.2% phosphoric acid solution (45∶55). The detection wavelength was 220nm. RESULTS : The average recoveries of oxymatrine and matrine in Compound Shiwei Tablets were 100.3% and 100.6% , respectively and in Radix Sophorae Flavescentis were 101.0% and 101.2%, respectlively. CONCLUSION: This method could be used for the quality evaluation of Compound Shiwei Tablets and Radix Sophorae Flavescentis.

OBJECTIVETo search for an optimal protocol and freezing conditions for the cryopreservation of microamount round spermatids of the mouse.

METHODSWe compared the survival rates of frozen-thawed microamount round spermatids of the mouse achieved by vitrification or standard slow freezing with different concentrations of glycerol (5, 7, or 9%) and different lengths of equilibrium time (0, 15, 30, 45, or 60 min).

RESULTSUnder the conditions of 7% glycerol and 30 min equilibrium, both vitrification and standard slow freezing achieved high survival rates of spermatids, and the former obtained an even higher rate than the latter ([72.9 ± 15.4]% vs [58.2 ± 17.7]%, P < 0.05).

CONCLUSIONA high rate of frozen-thawed microamount round spermatids of the mouse can be achieved by vitrification under the conditions of 7% glycerol and 30 min equilibrium.

Animals ; Cell Survival ; Cryopreservation ; methods ; Cryoprotective Agents ; administration & dosage ; pharmacology ; Glycerol ; administration & dosage ; pharmacology ; Male ; Mice ; Spermatids ; Time Factors ; Vitrification ; drug effects

Objective To evaluate the value of CT in the diagnoses and treatment of the liquid in the renal sub capsule.Methods The spiral CT images and clinic material of the liquid in the renal sub capsule were concluded in 25 cases.Results The total accuracy of CT showed 100% for the liquid in the renal sub capsule and reliable for the injury of liver and spleen,fracture of lumbar vertebra and abdominal accumulation of water.Conclusion CT diagnoses is the evidance to choose treatment for the liquid in the renal sub capsule with conservation,intervention,or operation.

Concurrent chemoradiotherapy is the optimal regimen for patients with inoperable locally advanced non-small cell lung cancer (LA-NSCLC).The application of new technologies such as positron emission tomography (PET)/CT and four dimensional CT (4D-CT) enhances the accuracy of radiotherapy and decreases adverse reaction.Induction chemotherapy and consolidation chemotherapy do not show benefit to survival.The targeted therapy and immunotherapy have the potential of improving the outcomes of inoperable LA-NSCLC.

Objective To investigate the influence of nebulized Pulmieort respules inhalation after endoscopic sinus surgery (ESS)on the expression of epithelial Na~+ channel(ENaC)protein in nasal mucosa. Methods Forty-four patients with nasal polyps undergoing ESS were randomly divided into Pulmieort respules treatment group(n=21,nebulized Pulmieort respules inhalation for 10 d after ESS)and Rhinocort control group(n=23,Rhinoeort aqueous nasal spray for 10 d after ESS).All the patients were performed biopsy of membrane on the residual middle turbinate 14 d after ESS,eosinophils (Eos)and neutrophils(Neu)per hundred inflammation cells were counted under microscope during ESS and after ESS,and the expression of ENaC protein was detected by immunofluorescence assay. Results The percentages of Eos and Neu decreased in two groups after treatment,and the percentage of Neu in Pulmieort respules treatment group was significantly lower than that in Rhinoeort control group(P<0.05).The expression of ENaC protein after treatment in Pulmieort respules treatment group was significantly lower than that in Rhinoeort control group(P<0.01). Conclusion Application of pulmieort respules after ESS can decrease Neu infiltration and inhibit expression of ENaC protein,which can relieve acute inflammation and edema of nasal mucosa.

Objective: To study the effect of Tang Ke Decoction on tumor necrosis factor ?(TNF?) and glucose transporter 4(GLUT4) expression in tissues of model rats of insulin resistance of type two diabetes mellitus,and to investigate the mechanism of the function of Tang Ke Decoction to insulin resistance.Methods: Rat model of insulin resistance of type two diabetes mellitus was duplicated by using strepozotocin(STZ) and high caloric diet,and using Tang Ke Decoction of compound Chinese herbs and rosiglitazone acted on model rats individually.The degree of insulin resistance was measured by insulin sensitivity index(ISI).The expression of TNF? mRNA was assayed in adipose tissue by RT-PCR.GLUT4 protein expression was assayed by immunohistochemical method in skeletal muscule tissue,and its distribution in skeletal muscle cell was observed with electron microscope.Results: ISI of model group was significantly lower than that of control group(P

AIM: To set up population pharmacokinetics/pharmacodynamics (PPK/PD) model of valproate (VPA) in children with epilepsy in China, and promote reasonable use of antiepileptic drugs(AEDs)in clinical practice. METHODS: Sparse data of VPA serum concentrations from 246 pediatric children were collected. These patients were divided into three groups: PPK-Model group ([WTBX]n=146), to calculate PPK parameter values of VPA and set up a PPK model; PPK-Valid group ([WTBX]n=100), to valid the PPK model; and PPD group ([WTBX]n=69), to set up PPK/PD model. Based on the data of PPK-Model group and PPK-Valid group, a PPK model of VPA in children with epilepsy in China was successfully set up by using NONMEM software by ourselves. Now, using the data of 69 patients in PPD group who were on VPA monotherapy and this PPK model, we set up PPK/PD model by NONMEM software. Efficacy of epilepsy treatment was divided into 5 grades according to the percentage of seizure frequency decreased (PSFD%): grade 1: PSFD% was 100%; grade 2: PSFD% was 75%-100%; grade 3: PSFD% was 50%-75%; grade 4: PSFD% was 25%-50%; grade 5: PSFD% was less than 25%. The quantitive relationship between the VPA serum concentrations and the probability for its efficacy score was characterized by Logistic regression analysis with NONMEM. RESULTS: Logistic regression analysis showed that, VPA serum concentrations and the probability for its efficacy grades 5, 4, 3, 2, and 1 were (23 ?g?ml -1, 5, 50%), (30 ?g?ml -1, 4, 32.3%), (50 ?g?ml -1, 3, 26.3%), (65 ?g?ml -1, 2, 36.5%), (78 ?g?ml -1, 1, 50%), and (100 ?g?ml -1, 1, 84.2%)respectively. CONCLUSION: A PPK/PD model of VPA in children with epilepsy in China is successfully established by using NONMEM software, and the probability of efficacy grade for any concentration can be calculated. It will be valuable to facilitate individualized dosage regimen.

Objective To design a handy labor monitor based on ARM hardware structure so that the condition of pregnant women can be reflected more accurately and completely.Methods The host-slave mode was adopted,which was composed of host machine based on ARM and embedded WinCE operating system.The slave machine used the 51 single chip to acquire data.Results This system was convenient and stable,and the interface is friendly.Conclusion The study of this system can be used for reference for the development of related data acquisition machine.[Chinese Medical Equipment Journal,2008,29(2):31-33]

Objective To assess the value of intraoperative ultrasonography during the uterus septum resection under a hysteroscope.MethodsThe uterus septum resection was performed under the guidance of transabdominal ultrasonography by using a hysteroscope in 30 patients.The operative procedure and the position of the hysteroscope were monitored,and the depth of the dissection and thickness of the myometrium in the fundus uteri were measured with transabdominal ultrasound during the operation.ResultsThe thickness between the incisal surface and serosal layer in the fundus uteri was within the normal limits in 28 cases.In 2 cases,the tissue was over-ectomized.The position of the urethral catheter balloon was correct in all of the 30 cases.Three months after the procedure,reexamination by transvaginal ultrasonography showed that the uterine cavity was normal in the patients.ConclusionsIntraoperative ultrasonography is quick,safe and precise for monitoring uterus septum resection.It is valuable for establishing surgical strategy and reducing operative complications.

Objective: Various quantitative assessments of coronary heart disease(CHD) risk factors was performed to evaluate severity and predicting its occurrence rate.Methods:282 cases,were selected to be scored by Framingham,PROCAM(The Prospective Cardiovascular Munster Study) and Chinese risk scores respectively.We compared the relationship between three risk scores and severity,extent of coronary artery through correlation analysis,and subsequently analyzed their predictive value.Results: Chinese risk score,PROCAM risk score,Framingham risk score were significantly correlated with extent and severity of coronary artery atherosclerosis(CAAS),with PROCAM risk score,the highest correlation of it and Framingham risk score,the smallest.Risk scores increased with coronary artery pathological change advancing.The areas under resident operation character(ROC) of Framingham risk score was the least,whereas that of PROCAM was the largest.Conclusion: Among the three risk scores described above used to evaluate the severity of CAAS and predict incidence of CHD,PROCAM risk scores was the superior.