Vascular endothelial growth factor (VEGF) was proved to be closely associated with neovascularizational ocular diseases and a new therapeutic target.Anti-VEGF agents can inhibit the neovascularization and decrease the permeability of blood vessels.The application of anti-VEGF agents has revolutionized the treatment of these diseases.Ranibizumab has been approved to apply for the treatment of wAMD in China.In USA,it has been also approved to apply for the treatment of macular edema induced by diabetes or retinal vein occlusion.As off-label drug use,anti-VEGF agents also are used for other ocular neovascular diseases,such as proliferative diabetic retinopathy,other retinal vascular diseases and neovascular glaucoma.The clinical application of anti-VEGF agents as an adjunctive treatment modality to surgery has shown the obvious therapeutic effect,including minimizing the risk of complication and recrudescence in intraoperation and posteoperation.The perioperative research and application of anti-VEGF drugs including bevacizumab and ranibizumab in retinal vascular diseases were reviewed.

Objective: To quantitative analyze the expression of osteopontin(OPN) in oral squamous cell carcinoma(OSCC) tissues and its clinical significance.Methods: OPN expression was examined in 59 OSCC(with different clinical and pathological characteristics) and 12 normal oral mucosa(NOM) samples by using immunohistochemical method.The staining results were quantitatively studied by image analyzer.Results: OPN expression was much higher in OSCC sample than that in NOM sample(P

Ageing of population is now a phenomenon of the world and has considerable consequences for public services.Ageing is always accompanied with dysfunction of multiple organs,including hypothalamic-pituitaryadrenal axis.Here,we summarized alterations of hormone secretion of adrenal glands in the elderly and their relationships with Alzheimer' s disease.Moreover,we discussed characteristics of pheochromocytoma,primary aldosteronism,and Cushing's syndrome in the elderly.Lack of typical manifestations usually makes difficulty in diagnosis.

Objective To investigate the injury effects and mechanisms of circulating histones on the hepatocytes in patients with hepatitis B virus (HBV )-related liver failure (HBV-LF) .Methods Serum samples from patients with HBV-LF were collected . The levels of serum histone H3 , histone H4 , prothrombin activity (PTA ) ,total bilirubin (TBil) ,creatinine (Cr) and international normalized ratio (INR) of the patients were measured .Model for end-stage liver disease (MELD) score was calculated in the patients .The serum levels of histones were compared between patients and the healthy volunteers . The correlation of histones with the MELD score and PTA was analyzed .The human liver cell line L-02 cells were cultured and treated with the serum of patients or L-02 cellular lysate supernatant preincubated with or without anti-histone H3 and H4 antibodies .The cellular morphology and rate of apoptosis were observed .Intracellular calcium ion concentration and Caspase-3 activity were detected in the cultured L-02 cells treated with histones .Mean of two independent samples was compared using t tests .Relationship between histones and the MELD score or PTA was conducted using Spearman correlation analysis .Results The levels of serum histones in the patients with HBV-LF were much higher than those in the healthy volunteers (H3 :[5 390 .3 ± 1 032 .0] μg/mL vs [42 .7 ± 12 .8] μg/mL , t = 32 .76 , P < 0 .01 ; H4 :[4 205 .1 ± 662 .3] μg/mL vs [40 .3 ± 14 .6] μg/mL ,t = 39 .74 , P< 0 .01) .In addition ,serum histones (H3/H4) levels in patients were negatively correlated with serum PTA (r= - 0 .325 ,P= 0 .038 and r =- 0 .572 ,P= 0 .028 ,respectively) ,but positively correlated with the MELD score (r= 0 .359 ,P= 0 .021 and r = 0 .568 , P = 0 .007 , respectively ) . Both serum of patients with HBV-LF and L-02 lysate supernatant were toxic to cultured L-02 cells .The injury effect was inhibited by anti-histone antibodies ([9 .3 ± 1 .5]% vs [14 .3 ± 0 .6]% , t = 4 .259 , P= 0 .02) .L-02 cells treated with calf thymus histone were cultured for 4 h . Cellular toxicity of histones resulted in Caspase-3 activation . The effect was inhibited by anti-histone antibodies ([3 .5 ± 0 .5]% vs [5 .2 ± 0 .6]% ,t= 4 .243 ,P= 0 .02) .Conclusion The elevated circulating histones in the patients with HBV-LF may aggravate the liver damage .

Objective_To identify the anti-HBV efficiency of AAV-IFNαby infecting HepG2.2.15 cell line, and to develop a new strategy of curing hepatitis B.Methods_Firstly,recombinant vector was packaged into AAV by three plasmids co-transfection method;then AAV-IFNαinfected HepG2.2.15 cell line, and samples were collected on the 1st, 3rd,6th and 9th day;thirdly, The time-course of HBV DNA,HBsAg and HBeAg were detected by qPCR and ELISA.The proliferation of HepG2.2.15 cells detected by MTT.Results_Recombinant AAV-IFNαcan significantly inhibit HBV replication and expression in vitro because of the proliferation of HepG2.2.15 cells inhibited by AAV6-IFNα( P<0.05) .Conclusions_AAV-IFNαis a potential antiviral product for hepatitis B.

Objective To analyze the short term prognosis of very low birth weight (VLBW) infants. Methods The clinical data of VLBW infants who were hospitalized from December 2013 to December 2014 and followed up to 6 months of corrected age were retrospectively analyzed. Results A total of 230 VLBW infants were enrolled. Thirty infants (13.0%) died during hospitalization, 40 infants (17.4%) were given up the treatment, 60 infants (26.1%) were improved at discharge, and 100 infants (43.4%) were cured at discharge. In the 30 cases of death, causes of death were mainly neonatal respiratory distress syndrome (18 cases), pulmonary hemorrhage (5 cases), and sepsis (3 cases). In the 200 survived infants at discharge, at 6 months of corrected age of follow-up, 13 infants (6.5%) were lost to follow-up, and 54 infants (27.0%) died, among whom 40 infants died because of giving up therapeutic intervention due to economic factor or discharges without permission. At 1, 3, 6 month of follow-up, the length and weight of VLBW infants were gradually approaching normal, and, however, at 6 months of corrected age, the length and weight of VLBW infants were still obviously below the children’s growth standard. The length of VLBW infants was more deeply below the growth standard than the weight. In 109 infants who had fundus ophthalmoscope, 21 cases had retinopathy of prematurity (ROP) I, and 7 cases had ROP II. In 98 infants who had the hearing screening test, 5 cases failed in one ear and 11 cases failed in both ears. In 95 infants who had the head magnetic resonance imaging (MRI) examination, 10 cases had intracranial hemorrhage and 9 cases had premature brain injury. In 49 infants who completed the neonatal behavioral neurological assessment (NBNA) at corrected age of 42 weeks, the scores were all lower than 35. In 36 infants who were assessed by Gesell developmental scale at the 3 months of corrected age, 11 cases had mild developmental delay and 2 cases had moderate developmental delay. In 24 infants who were assessed at the 6 months corrected age, only 2 cases had mild developmental delay. VLBW infants had a rapid progress in social contact, social behavior, and gross motor movement. Conclusions Economic, neonatal respiratory distress syndrome, pulmonary hemorrhage, and sepsis are pivotal factors for the survival and quality of life of VLBW infants. The surviving VLBW infants have catch-up growth and development.

Objective To explore the clinical efficacy and safety of mouth three oxide inorganic polymer under a microscope to repair the pulp cavity perforation.Methods 120 patients with medullary cavity perforation, caries origin were selected as the research subjects,they were randomly divided into oral microscope inorganic oxide polymer repair the pulp cavity perforation of the experimental group and the resin inlay repair the pulp cavity perfora-tion of the control group,each group had 60 cases.The postoperative occlusion pain,swollen gums and loose,fistula formation,the incidence of chewing function not completed and X -ray curtate alveolar bone density decrease the shadow changes were compared between the two groups.Results The incidence rates of bite pain,swollen gums, loosening of the observation group were 5.00%,3.33%,6.67%,which of the control group were 11.60%,13.33%, 18.33%,the differences between two groups were significant (χ2 =5.49,P <0.05;χ2 =4.76,P <0.05;χ2 =4.23, P <0.05).The fistula formation,chewing function not completed,X -ray curtate alveolar bone density to reduce shadow expand of the observation group were 3.33%,5.00%,5.00%,which of the control group were 10.00%, 30.00%,40.00%,the differences between the two groups were significant (χ2 =5.34,P <0.05;χ2 =4.12,P <0.05;χ2 =4.56,P <0.05).Conclusion The clinical curative effect of oral three oxide inorganic polymer under a microscope to repair the pulp cavity perforation is outstanding,compared with the resin inlay repair the pulp cavity perforation has many advantages,it can be used as a kind of ideal method used in the clinical work.

Objective To investigate the activity of gastrocnemius muscle after acellular nerve transplantation with microencapsulated peripheral nerve tissue to repair sciatic nerve gap in dogs. Methods Twelve dogs were selected and divided randomly into four groups with three in each. Both sciatic nerves of the dogs in group A were cut and chemically extracted to make acellular nerves. Acellular nerves were transplanted into the dogs of groups B and C to bridge 30 mm sciatic nerve gaps, and additionally microencapsulated peripheral nerve were added in group B. Autograft was used for repairing the defects in group D. The motor function of operated limbs were observed regularly after operation. Six months after operation, motor conduction velocity of the specimens of sciatic grafting nerve segment were observed, and the gastrocnemius muscle of both sides were obtained and performed succinic dehydrogenase (SDH), synaptophysin (SYP) and motor end plate histochemical staining, and sciatic nerve 1 cm from distal end stoma were obtained and underwent Masson, Luxol fast blue and NF200 staining. Results All the dogs in groups B, C and D were involved in the results analysis without loss. Image analysis indicated the optical density and the cross section area of gastrocnemius muscle, area and optical density of synaptophysin, area and optical density of motor end plate in group B showed statistically significant difference compared with those in group C, but no significant difference with those in group D. The results of motor function recovery of operated-limb, electrophysiological examination and morphologic observations of sciatic nerve were all consistent with the detections of gastrocnemius muscle. Conclusion The gastrocnemius muscle may be again innerved by nerve, and the muscle atrophy is significantly alleviated after acellular nerve transplantation with microencapsulated peripheral nerve tissue for repairing sciatic nerve gap. The regeneration of nerve was better after the combined transplantation than only acellular nerve transplantation, so the combination of acellular nerve and microencapsulated peripheral nerve tissue may be a useful substitute of autograft for nerve repair.

Objective To propose the orientation,goals and some key issues for future research in the field of war wound/trauma through reviewing the updated literature.Methods The latest literature in the field of war wound/trauma were reviewed.Results The notion "medicine always be with soldiers" had been raised by America and the Europe military circles,with special emphasis on emergency techniques and equipments,which were used in the front of battle field.This forwarded the medical aids as quick as possible and the formation of medical rescue system without crack.Meanwhile,much attention had been paid to the pathogenesis and prevention of post-traumatic complications,wounding characteristics and mechanisms of high-tech new weapons,as well as repair and regeneration of injured tissues.In China,a great advances had been made in the filed of emergency rescue,shock resuscitation,complication treatment and wound repair.Conclusions During the coming "twelfth five-year program",the research standpoint is to meet the needs of Chinese troops to perform better in modern wars and non-military actions,and the aim is to significantly reduce the rate of mortality and disability of trauma as well as to improve the whole army's health.To fulfill this aim,a lot of work should be done to enhance the trauma emergency ability at the battle front,the organization and remedy ability at conditions of combined operations of armed forces,and the translational medical research for rescue of trauma,so as to unceasingly increase the medical service ability of our army to cope with secure threaten and to accomplish multiple military missions.

OBJECTIVE: To investigate the tagging condition in medication of special population in package inserts in order to give reference for rational drug use of special population. METHODS: 389 pieces of common Chinese and western medicine package inserts of our inpatient and out-patient dispensaries were randomly collected and analyzed statistically in terms of the content of package inserts for special population, including pregnant and lactant women, children and the aged. RESULTS: A part of medication items of special population in package inserts had deficiency. According to the classification of dosage form, the tagging rate of injection exceeded other dosage forms reached to more than 90%, except of medication items for children. The tagging rate of oral formulation arrived at more than 90% only in terms of contraindications and cautions. According to the classification of manufacturer, the tagging rate of domestic drugs was kept at the low level while that of imported drugs exceeded 90%. CONCLUSIONS: Package inserts need to be improved with the effort of drug supervision management department, drug production department and population of using drugs to guarantee the safety of drug use in special population.