Miao medicine has a relatively complete system and the methods of Miao medical therapy have distinctive ethnic characteristics.Through extensive research we summed up the three methods Condotherapy,external treatment,and Qi therapy;therapy and the seven features of Miao medical therapy,many of which are worth learning and lucubrating.

Whether low dose of endocrine disruptors(EDs) have adverse effects on human health is a hot spot in recent ten years.Many researchers consider that EDs lower than non-observed adverse-effects level can produce biological effects.In this paper the author expatiated the concept and mechanism of low dose effect of EDs,summarized some noso-biological-effects of low dose EDs to children.At the same time,some arguments of this field were introduced.

Background and purpose:Actin related protein 2/3 complex subunit 5 (ARPC5) is involved in the packaging of actin, and then affects the mobility of cells. It plays an important role in the formation of invadopodia of cancer cells. This study aimed to investigate the effect of ARPC5 gene-silence on cell proliferation, migration and invasion of lung squamous carcinoma cell line SK-MES-1. Methods:Under the induction of LipofectamineTM RNAiMAX, the recombinant of si-ARPC5 was transfected into SK-MES-1. The experiment set up 3 groups:blank (mock) group, negative control (NC) group and si-ARPC5 transfected group. Cell proliferation, migration and invasion assays were investigated by cell counting kit(CCK-8) method, scratch assay and Transwell Chambers respectively. Results:The result of qRT-PCR and Western blot displayed downregulation of ARPC5 in mRNA and protein level of SK-MES-1 cell. Proliferation rate of SK-MES-1 cells in 72 h after si-ARPC5 transfection was lower than those in mock and NC groups (t=7.993, t=8.681, P<0.05). Compared to the mock and NC groups, the scratch repairing rate of the si-ARPC5 group was lower [(43.32±0.23)%vs (73.11±0.43)%and (76.58±0.88)%] (t=7.348, t=10.614, P<0.05);And the number of penetrating the membrane was signiifcantly reduced [(27±6) vs (101±11) and (92±9)] (t=10.229, t=8.391,P<0.05). Conclusion:Downregulation of ARPC5 gene can inhibit the proliferation, migration, and invasion of SK-MES-1 cell.

The establishment and perfection of the assessment system for chronic liver diseases have a guiding significance for clinical diag-nosis and treatment.Hepatic venous pressure gradient (HVPG)is of great significance in the progression of chronic liver diseases,and it is the only pathophysiological index for treatment independent of etiology.The current methods for evaluation of chronic liver diseases are sim-ply described,and the application of HVPG in evaluation of chronic liver diseases is systematically summarized,including predicting the se-verity of liver cirrhosis,variceal bleeding risk and outcome of portal hypertension,development of cirrhotic ascites,efficacy of drugs for re-ducing portal hypertension and antiviral drugs,and postoperative outcome of liver cancer.It is thought that HVPG measurement plays an im-portant role in the assessment of the progression and prognosis of chronic liver diseases and is an effective predictor of outcome.

With development of molecular biology,breast cancer has entered an era of molecular classifi-cation,thus making biomarker based personalized medicine is the trend of breast cancer treatment.HER2-posi-tive breast cancer of high invasiveness and bad prognosis accounts for 20%~30%.Targeting HER2,trastuzumab is the first humanized monoclonal antibody which can improve the prognosis of HER2-positive patients and it is recommended by guidelines and expert consensus at home and abroad for anti-HER2 therapy in any stage.How-ever,the cardiotoxicity,de novo resistance and acquired resistance of trastuzumab make the clinician to explore the second line anti-HER2 therapy.Lapatinib is the first FDA approved and HER1,HER2 double-targeting tyrosine kinase inhibitor which can be a better choice after failure with trastuzumab.This article reviews the appli-cation,some clinical and mechanism of drug resistance researches of Lapatinib.

Objective To study the impact of mindfulness therapy on the surgical stressors of patients undergoing elective surgery. Methods One hundred and twenty patients undergoing elective surgery were randomly divided into the observation group and the control group according to the random number table with 60 in each group. The control group was treated with conventional care intervention and the former with nursing intervention based on mindfulness therapy, body scanning, mindful breathing, mindfulness meditation, walking meditation, mindfulness yoga and mindfulness aspect introspection, besides the conventional one. The intervention effect was compared between the groups by way of the Chinese version perceived stress scale (CPSS), self-rating anxiety scale (SAS) and self-rating depression scale (SDS). Result After the intervention, the scores of the observation group by CPSS, SAS and SDS were all significantly lower as compared to those of the control group (all P<0.05). Conclusion Mindfulness therapy can effectively reduce the level of perceived stress in patients undergoing elective surgery and relieve their anxiety and depression , which is conducive to the smooth operation and improvement of the operative quality.

Quality consciousness is the guidelines of the graduate cultivation.The perfection of organization and management as well as the strength of innovation and reform also immediately influence the training quality of graduates.Today the graduate scale expands,so we should use scientific development concept to guide the graduate cultivation and to develop postgraduate education quickly and soundly.

Wegener's granulomatosis (WG) is a multisystem disease characterized by necrotizing granulomatous inflammation and vasculitis. WG classically involves the upper airway, lung and kidneys. Otolaryngologic manifestations are often the presenting symptoms in WG, Subglottic stenosis is a less common, but a life-threatening manifestation of WG, This paper studies about the laryngeal and tracheal stenosis diagnosis and surgical treatment of Wegener's granulomatosis in the past 20 years.
Granulomatosis with Polyangiitis ; complications ; physiopathology ; Humans ; Laryngostenosis ; etiology ; physiopathology ; Larynx ; physiopathology ; Tracheal Stenosis ; etiology ; physiopathology

Keloid refers to the damaged skin due to excessive fibroblast proliferation. Ear is one predilection site. The pathogenesis of ear keloid is not very clear, and the treatment is also varied. Surgery, postoperative radiotherapy and laser treatment, steroid hormones, pressure therapy are the basic treatment methods. Integrated application of a variety of treatments, classification research and new materials using revealed the prospect for the treatment of the disease. This thesis reviews literature about ear keloid in recent 10 years, and introduces this disease and clinical research progress.
Cell Proliferation ; Ear ; pathology ; Ear Diseases ; pathology ; therapy ; Fibroblasts ; cytology ; Humans ; Keloid ; pathology

Objective:This study aimed to investigate the role of mitochondrial fusion protein 2 (Mfn2) in chronic renal injury caused by cyclosporine A (CSA) and the possible mechanism.Methods:The model of chronic CSA nephropathy was established in rats. The rats were divided into solvent control group, CSA model group and normal control group. Serum creatinine and urinary protein levels were detected. The tubulointerstitial injury was observed by light microscopy. The changes of mitochondrial structure were observed by electron microscope, and the expression of Mfn2 was detected by Western blot.Results:The levels of serum creatinine and urinary protein in CSA model group at 2 and 4 weeks were significantly higher than those in control group and solvent group ( P<0.05). In CSA model group, tubulointerstitial injury was obvious, mitochondria swelling and deformation were found in tubular epithelial cells, and ridge disappeared. Compared with normal control group and solvent control group, the expression of Mfn2 in CSA model group was significantly decreased at 2 and 4 weeks ( P<0.05), and the expression of Mfn2 in CSA model group at 4 weeks was further decreased compared with 2 weeks ( P<0.05). Conclusions:Mfn2 may play an important role in the injury of tubular epithelial cells caused by cyclosporine.