Objective To evaluate the validity and reliability of social avoidance and distress scale (SAD) for conforming it' s applicability in Mongolian college students.Methods Social avoidance and distress scale (SAD)security scale (SQ),self-esteem scale (SES) and life satisfaction scale (AULC) was used to measure 399 undergraduates whose can understand Mongolian and Chinese language.Results The total scale reliability was 0.872,avoidance scale was 0.726,distress scale was 0.742,total scale heavy reliability (r) was 0.86,avoidance scale (r) was 0.789,distress scale (r) was 0.814.(2)the confirmatory factor analysis verified the effectiveness of the structure,the revised model fitting index x2/df=4.864,NFI =0.92,TLI =0.90,GFI =0.90,IFI =0.87,GFI =0.92,AGFI =0.93,RMSEA =0.06,model fitting was better.(3) There was a significant positive correlation between avoidance scale and distress scale (r=0.634,P＜0.01),and there was a negative correlation (r=0.202-0.499,P＜0.01) between avoidance scale and SES,SQ and AULC.There was a negative correlation between distress scale and SES,SQ and AULC (r=0.192-0.556,P＜0.01).Conclusion SAD (Chinese version) has a good reliability and validity in Mongolian college students.

BACKGROUND:Studies have shown that bisphosphonates can act on osteosarcoma cels, but the comparative analysis of bisphosphonates and cisplatin, a traditional first-line chemotherapy drug, is rarely reported. OBJECTIVE:To investigate the differences between bisphosphonates and cisplatin to inhibitin vitroproliferation of osteosarcoma cels and induce cel apoptosis. METHODS: Subcultured MG-63 cel lines were intervened with different concentrations of bisphosphonates and cisplatin, respectively, and cels with no treatment served as negative controls (blank group). Cel inhibition rate was detected using MTT method, and cel morphology was observed using fluorescent staining. RESULTS AND CONCLUSION:After intervention with different concentrations of bisphosphonates and cisplatin for different time, the growth inhibition rate of MG-63 cels was significantly higher as compared with the blank group (P < 0.05). But there was no difference in the growth inhibition rate between the bisphosphonates and cisplatin groups at 24, 48 and 72 hours (P > 0.05). These findings indicate that bisphosphonates has obvious inhibitory effect on the growth of osteosarcoma celsin vitro, and its inhibitory effect is similar to that of cisplatin.

Objective To explore the role of uncoupling protein 2 ( UCP2) in the development of hepatic fibrosis and its molecular mechanism .Methods The CCl4-induced liver fibrosis rat model in vivo was established to observe the pathological changes of rat livers .The expression levels of UCP2 and p38 mitogen activated protein kinase (p38 MAPK) were detected by using the techniques of Western blotting , Real-time PCR and immunohistochemistry .The hepatic stellate cells ( HSC) were stimulated by CCl 4 and UCP2-specific inhibitor Genipin to mimic liver fibrosis in vitro.The expression levels of UCP2 and p38MAPK were determined by using Western blotting .Results We found that UCP2 and α-SMA expression levels increased significantly (P <0.05, n =10) in the liver of rats with CCl4-induced liver fibrosis when compared with that of the normal control rats in vivo.Similarly, the expression levels of UCP2 and p38 MAPK were up regulated (P <0.05, n=6) in CCl4-treated HSC cells in vitro.However, the expressions of UCP2 and p38 MAPK were down regulated (P <0.05, n=6) in genipin-treated HSC cells in vitro.Conclusion UCP2 is involved in liver fibrosis, and probably contributed to the activation and proliferation of hepatic stellate cells .

Objective To research the expression of mitofusin-2 (mfn2) and tumor metastasis suppressor genes (nm23)in bladder cancer cells and its correlation with clinical pathological feature. Methods Immunohistochemistry was used to measure the expression of mfn2 and nm23.Sixty-five cases of bladder cancer were sampled,which include fif-ty cases of male and fifteen cases of female. TNM stage:Forty-seven cases were in stage I;Ten cases were in stage II; Five cases were in stageⅢ; Three cases were in stageⅣ. Other fifteen cases were sampled from normal bladder or benign tumor of bladder as control . All cases were collected from department of pathology,affiliated Hospital of hebei union university. Results The positive expression rate of mfn2 in bladder cancer tissues was significantly higher than those in normal blad-der tissues and benign tumor of bladder(χ2=32.528,P<0.05);The positive expression rate of nm23 in bladder cancer tis-sues was significantly lower than those in normal bladder tissues and benign tumor of bladder (χ2=19.719,P<0.05);the high expression level of mfn2 in bladder cancer was associated with tumor differentiation and TNM stage(P<0.05),but not corre-lated with age,sex,lymph node metastasis and clinical grade. The low level of nm23 was associated with TNM stage,clinical grade and LN metastasis. Conclusion The positive expression rate of mfn2 was increased in bladder cancer. It indicated that there was a close relationship between mfn2 and the occurrence and development of bladder cancer;The expression rate of nm23 was decreased in bladder cancer,it may be a predictor for metastasis and prognosis of bladder cancer.

Objective To study the effect of automobile exhaust pollution saturation of carboxyhemoglobin (COHb) in traffic policemen. Methods 169 traffic policemen working outdoors were selected as a exposure group and 112 traffic policemen working indoors as control group in a traffic policemen detachment in Tangshan. Questionnaires and health examination were conducted in two groups. The carbon monoxide levels in the exhalation were measured in 281 traffic policemen by a micro_smokerlyzer and the corresponding saturation of carboxyhemoglobin (COHb) were read. Results The higher saturation of carboxyhemoglobin were observed in exposure group (2.30%?1.49%) compared with those (1.71%?0.99%) in control group (P

Objective The purpose of this study was to evaluate the effect of prostaglandin E 1 (PGE 1) on blocking the development of acute respiratory distress syndrome (ARDS) induced by acid aspiration. Methods Twenty new Zealand rabbits were used. Dilute HCl was instilled into right bronchus of the rabbits. The rabbits were then divided randomly into two groups: injury group and treatment group. In injury group (n=10) rabbits received no treatment except mechanical ventilation. In treatment group (n=10) immediately after acid instillation the rabbits received an intravenous bolus of PGE 1 followed by a continuous infusion. Blood gas, airway pressure and dynamic and static compliance were measured before and after acid instillation. Blood samples were taken from artery for determination of 6-k-PGF 1?, TXB 2,NO 2 -/NO 3 - and ET-1. The animals were killed and the wet/dry lung weight (W/D) ratio and total protein of bronchoalveolar lavage fluid(BALF) of right lung were measured. Microscopic examination of the lung was done. Results In treatment group PaO 2 was significantly higher than that in injury group after acid instillation. Plasma 6-k-PGF 1? and NO - 2/NO - 3 levels were significantly higher in treatment group while plasma TXB 2 and ET-1 levels were significantly lower. W/D ratio and TP of BALF of right lung were significantly lower in treatment group. The inflammatory changes were diffuse in injury group while in treatment group they were localized and less severe. Conclusions PGE1 can lessen severity of ARDS induced by acid aspiration. It may protect pulmonary vascular endothelial cells through maintaining the balance between PGI 2 and TXA 2 and that between NO and ET-1 .

MicroRNAs are endogenously expressed non-coding RNAs, which are composed of approximately 18 nucleotides to 25 nucleotides. Mature microRNAs regulate gene expression by base pairing with the 3'-untranslated region of target mRNAs. These mature microRNAs can degrade target mRNAs or inhibit translation. This process is a type of post-transcriptional regulation of gene ex-pression. Studies have shown that microRNAs are important in physiological and pathological processes, such as cell proliferation, cell differentiation, and cell death. This article provides an overview of the function of microRNAs in the regulation of macrophages.

ObjectiveThe purpose of this study was to evaluate the effect of prostaglandin E1 (PGE1) on blocking the development of acute respiratory distress syndrome (ARDS) induced by acid aspiration. MethodsTwenty new Zealand rabbits were used. Dilute HCl was instilled into right bronchus of the rabbits. The rabbits were then divided randomly into two groups: injury group and treatment group. In injury group ( n = 10) rabbits received no treatment except mechanical ventilation. In treatment group ( n = 10) immediately after acid instillation the rabbits received an intravenous bolus of PGE1 followed by a continuous infusion. Blood gas, airway pressure and dynamic and static compliance were measured before and after acid instillation. Blood samples were taken from artery for determination of 6-k-PGF1α, TXB2, NO2-/NO3- and ET-1. The animals were killed and the wet/dry lung weight (W/D) ratio and total protein of bronchoalveolar lavage fluid(BALF) of right lung were measured. Microscopic examination of the lung was done. ResultsIn treatment group PaO2 was significantly higher than that in injury group after acid instillation. Plasma 6-k-PGF1α and NO2-/NO3- levels were significantly higher in treatment group while plasma TXB2 and ET-1 levels were significantly lower. W/D ratio and TP of BALF of right lung were significantly lower in treatment group. The inflammatory changes were diffuse in injury group while in treatment group they were localized and less severe. Conclusions PGE1 can lessen severity of ARDS induced by acid aspiration. It may protect pulmonary vascular endothelial cells through maintaining the balance between PGI2 and TXA2 and that between NO and ET-1 .

Asthma, Occupational ; etiology ; pathology ; Humans ; Inflammation ; pathology ; Neutrophil Infiltration ; Neutrophils ; pathology

Asthma, Occupational ; therapy ; Drainage ; methods ; Humans ; Sputum