Objective:To explore the expression and significance of IKK, I?B and NF-?B P65 of NF-? B signal transduction pathway in laryngeal carcinoma patients.Methods:The expression of IKK?, I?B?,NF-?B P65 was examined by immunohistochemical S-P method in laryngeal carcinoma tissues of 40 cases and normal laryngeal tissues of 20 cases.Results:The expression of IKK? and NF-?B P65 in laryngeal carcinoma tissue was higher compared with normal laryngeal tissue,while the expression of I?B? was lower( P

Objective:To investigate the effects of siRNA-mediated Plk1 depletion on COS-7 cells and the therapeutic potential of Plk1 in cancer treatment.Methods:Small interfering RNA(siRNA) were used to specifically deplete Plk1 in COS-7 cells.RT-PCR and immol/Lunoblotting analysis were used to verify the inhibitory effect of siRNA against Plk1.Trypan blue exclusion assay and FACS analysis were conducted to determine the effects of Plk1 depletion on the growth and cell cycle profile of COS-7 cells.Results:Synthetic siRNA duplexes against Plk1 were introduced into COS-7 cells,which subsequently resulted in a significant inhibition in Plk1 expression in the cells.Cell growth assay indicated that siRNA-mediated Plk1 depletion significantly inhibited the proliferation of COS-7 cells.FACS analysis revealed that Plk1 depletion caused cell cycle arrest,resulting into large population of sub-G1 cells.Conclusion:siRNA-mediated Plk1 depletion significantly inhibited the proliferation of COS-7 cells suggesting the therapeutic potential of Plk1 in cancer treatment.

Objective:To investigate the expression and significance of NF-?B,and ICAM-1 in laryngeal carcinoma patients.Methods:Two groups of laryngeal carcinoma tissue (one group derived from the laryngeal carcinoma patients;the other from the normal persons) were detected for NF-kappa B P65 protein and ICAM-1′s expressions by immunohistochemistry staining.Results:(1)The expressions of NF-kappa B P65 protein and ICAM-1 of the laryngeal carcinoma patients were much more strengthened than those of the normal persons(P

Objective To evaluate the association between genetic polymorphisms in promoter regions of matrix metalloprotein‐ase‐1 (MMP‐1)‐1607bp1G/2G ,matrix metalloproteinase‐7 (MM P‐7)‐181‐bpA /G and susceptibility of cervical cancer using Meta‐analysis .Methods Relevant studies that had relationships of the polymorphism in promoter regions of matrix metalloproteinase‐1 (MM P‐1)‐1607bp1G/2G ,matrix metalloproteinase‐7 (MMP‐7)‐181‐bpA /G and susceptibility of cervical cancer were collected as case‐control groups .RevMan5 .0 software was applied to analyze the odds ratios (OR) and 95% confidence intervals(CI) of the as‐sociation between polymorphisms and susceptibility of cervical cancer .Results Finally ,6 studies were selected ,containing 4 studies of MMP‐1 and 2 studies of MMP‐7 .For MMP‐1‐1607bp1G/2G ,OR was 0 .76 (95% CI = 0 .51 - 1 .13 ,P= 0 .17) under 1G/1G +1G/2G ∶ 2G/2G model ,OR was 0 .72 (95% CI = 0 .42 - 1 .23 ,P = 0 .42) under 1G/1G ∶ 2G/2G model ,OR was 0 .79(95% CI =0 .57 - 1 .09 ,P= 0 .15) under 1G ∶ 2G model ,it was concluded that there was no statistical significance between them .For MM P‐7‐181bpA /G ,OR was 0 .47 (95% CI = 0 .30 - 0 .74 ,P= 0 .001) under AA + AG ∶ GG model ,OR was 0 .43 (95% CI = 0 .26 - 0 .74 , P= 0 .002) under AA ∶ GG model ,OR was 0 .77(95% CI = 0 .62 - 0 .94 ,P= 0 .02) under A ∶ G model ,there were statistical signifi‐cance differences under all models .Conclusion Polymorphisms in promoter of MMP‐7‐118bpA/G are associated with the suscepti‐bility of cervical cancer and an allele is a protective factor .It is still not confirmed that significant association between the MM P‐1‐1607bp1G/2G and susceptibility to cervical cancer exists in current literature .

Objective To investigate the application value of detection of serum miRNA-210,miRNA-23b in the patients with the femoral head necrosis.Methods The serum and clinical data of 135 patients with the internal fixation operation for the femoral neck fracture were collected.The patients were divided into the necrosis group (27 cases) and non-necrosis group (108 cases),used the real-time fluorescent quantitative PCR (Real time-PCR,RT-PCR) technology to detect serum miRNA-210,miRNA-23b expression in comparatively analysizing the relationship between the changes of serum miRNA 210,23b and occurrence of the femoral head necrosis.Results The serum microRNA-210,-23b expression in the necrosis group were 0.19±0.03,0.21±0.05,which in the non-necrosis group were 0.92±0.18 and 0.87±0.17.The serum microRNA-210,-23b expression in the necrosis group were significantly lower than those in the non-necrosis group,the difference had statistical significance (P＜0.01).The analysis results from the receiver-operating characteristic curve (ROC curve) showed that AUC of serum miRNA-210,miRNA-23b expression were 0.790 (95％CI:0.687～0.892,P=0.000) and 0.743 (95％CI:0.633～0.854,P=0.000),respectively.The sensitivity and specificity of miRNA-210 and miRNA-23b at the best threshold in diagnosis of the femoral head necrosis were 95.7％ and 93.9％,91.5％ and 92.3％,respectively.The analysis results showed that the serum miRNA-210,-23b expression had clinical significance in diagnosis of the femoral head necrosis.Conclusion Detection of serum miRNA-210,-23b expression can predict occurrence of the femoral head necrosis,estimate development condition of the desease.

Calcific aortic valve disease(CAVD) is the most common valvular disorder.There is no medical treatment to prevent and/or reverse the progression of CAVD and the etiology still requires further study.CAVD has long been described as a degenerative disease related to aging.In the past few years,our comprehension of the etiology and mechanisms leading to CAVD has progressed at a fast pace.In this article,we review the latest discoveries of CAVD,from aspects of lipid retention,inflammation,calcification,osteogenic transition and their basic molecular processes.

Objective To compare the mortality,valve-related morbidity and reoperation rate between mechanical prostheses and bioprostheses in patients aged 60 to 70 years.Methods The pre-operative characteristics of 119 patients who received mechanical prostheses and 181 those with bioprosthesis implantation in our department between January 2006 and December 2014 were analyzed,and their mortality,valve-related morbidity and reoperation rate were compared.Results Followup revealed mechanical prostheses had a lower all-cause mortality after mitral valve replacement than bioprostheses at 6 years after the operation (P=0.033),but there was no statistical difference in valve-related mortality (P=0.277).The complications after mechanical prosthesis replacement had embolism (8.4％) and bleeding (5.0％),bioprosthesis' those consisted of structural valvular deterioration (2.2％),nonstructural dysfunction (1.1％),thrombosis (2.8％),embolism (5.0％),bleeding (0.6％) and operated valvular endocarditis (1.1％).There was no statistically significant difference in total complication rate between the two groups (P=0.318).Biological group had 3 patients receiving reoperation in 8 years,while there was no reoperation in the mechanical group,but without statistical group difference.Conclusion Patients aged 60-70 years old could have a similar valve-related mortality,morbidity and reoperation rate after mitral valve replacement between mechanical and biological valves.

Epilepsy is a family of neurological disorders that are common and harmful to human's health. The etiologies of epilepsy and the clinical manifestations of seizures are complex and various. To elucidate the mechanisms underlying seizure generation, lots of researches in the anatomy, physiology, biochemistry, genetic and molecular biology of epilepsy have been done over many years. However, no single hypothesis provides a unifying framework within which all kinds of epilepsy can be included so far. In fact, the electrophysiologic essence of epileptic seizure is abnormal excess discharges of neurons. On the standpoint of electrophysiology, the reasons of abnormal discharges of neurons are of common character: depolarizing currents with long durations and high amplitudes on cell levels, which is closely related to slow waves with high amplitudes on EEG levels. This can make us understand the mechanism of epileptic seizures better, and is helpful for well control and therapy of seizures.

In order to improve the effect of teaching and teachers' ability,make our teaching methods adapt to the social development,the department of Endocrinology used the method of preparing lessons collectively in our teaching.We found that the method not only made the the supply of teachers reasonably but also made good contribution to the students' culture.Practice testified that the method of preparing lessons collectively could adapt to the new model of cultivation and create substantial results.

Objective:To make a synthetic comment on treatment of children cerebral palsy with acupuncture in recent 10 years in China.Methods:Related papers were gotten from the journals between 1999-2008 in Chinese journal full-test database and VIP information-Chinese technology journal database by computer retrieval.The collected 143 papers were analyzed from the terms of clinical acupoints selection,treatment methods,time,and mechanism,and then summarized the clinical effects of acupuncture in treating children cerebral palsy.Results:The acupoints selection was focus on scalp acupuncture functional area and body points(including scalp points).Beside acupuncture treatment,the combination of acupoint embedding therapy,acupoint injection,and massage were also used.The therapy time was different from 20min to 4h,and the treatment course was usually be counted by month.The effects of acupuncture treatment were considered to be significant through improving brain circulation and recovering brain cells' function. Conclusion:Acupuncture treatment had advantages in improving the child'limbs function,nevertheless further studies on the research methods,study design and mechanism were all needed.