Objective To explore the protective effect of hydrogen-rich saline on liver ischemiareperfusion (IR) in mice and the possible mechanisms. Methods Twenty-four C57BL/6 mice were randomly divided into 3 groups: sham-operated group, control group (mice were injected with 5 ml/kg saline by tail vein just before ischemia induction) and hydrogen-rich saline group (mice were injected with 5 ml/kg hydrogen-rich saline). Six hour after reperfusion, the mice were sacrificed and the serum and liver samples undergoing IR injury were collected. The ALT and AST levels in serum were determined and liver histiological damage was also evaluated with Suziki's criteria. Malondialdehyde (MDA) contents in liver samples were measured using specific kits. The infiltration of F4/80 positive macrophage cells was detected by using immunohistochemistry and that of neutrophils with myeloperoxidase (MPO) kits. The mRNA expression of TNF-α, IL-6, ICAM-1 and IP-10 was assayed by using real-time reverse transcription PCR. The activation of transcription factor NF-κB was measured by using Western botting analysis. Results As compared with control group, at the 6th h following reperfusion, mice in hydrogen-rich saline group exhibited lower levels of ALT and AST (P＜0. 05) in serum, milder histological damage (P＜0. 01) and less MDA contents in liver samples (P＜0. 01). The infiltration of macrophages, neutrophils and the mRNA expression of TNF-α, IL-6,ICAM-1 and IP-10 in the liver tissue in hydrogen-rich saline group were reduced as compared with IR group (P＜0. 05 or P＜0. 01). The activation of NF-κB in hydrogen-rich saline group was significantly down-regulated as compared with control group. Conclusion Injection of hydrogen-rich saline via the tail vein can alleviate liver IR injury probably by inhibiting oxidant stress and inflammatory response induced by reperfusion.

Objective:To study the therapeutic value of Xueshuantong injection combined atorvastatin in patients with coronary heart disease (CHD)complicated diabetes mellitus (DM).Methods:A total of 100 CHD+DM patients, who were treated in our hospital from Jan 2012 to Jan 2014,were selected.According to random number method, they were randomly and equally divided into routine treatment group (including atorvastatin treatment)and Xue-shuantong combined atorvastatin group (combined treatment group).Clinical therapeutic effect,change of blood lipid level and safety were observed in two groups.Results:Total effective rate of combined treatment group was significantly higher than that of routine treatment group (96.0% vs.68.0%,P =0.032).Compared with routine treatment group,after treatment,there were significant reductions in levels of total cholesterol [(4.38 ± 0.50) mmol/L vs.(4.09±0.47)mmol/L],triglyceride [(2.23±0.35)mmol/L vs.(1.79±0.28)mmol/L]and low density lipoprotein cholesterol [(2.45±0.29)mmol/L vs.(2.23±0.28)mmol/L],and significant rise in level of high density lipoprotein cholesterol [(1.11 ±0.12)mmol/L vs.(1.37± 0.25)mmol/L]in combined treatment group,P <0.05 all.Incidence rate of adverse reaction was 6.0% and 4.0% in combined treatment group and rou-tine treatment group respectively,but there was no significant difference between two groups,P =0.436. Conclu-sion:Xueshuantong combined atorvastatin treatment can significantly improve blood lipid levels and raise clinical therapeutic effect in CHD+DM patients.

0.05) in tail flick test.MPE% in group MK was always higher than group M and descended more slowly than group M,especially from the d4 to d8(P0.05). Conclusion Ketamine could block the development of morphine tolerance partly due to its inhibition effect on pCREB protein.

Objective To evaluate the efficiency of dexmedetomidine combined with flurbipro-fen axetil preventing agitation and reducing extubation reaction after general anesthesia. Methods Eighty patients,ASA Ⅰ or Ⅱ,scheduled for selective oral and maxillofacial surgery were randomly divided into four groups,20 patients in each group.30 mins before end of the operation, patients intravenously received flurbiprofen axetil 50 mg (group F),dexmedetomidine 0.5 μg/kg (group D),dexmedetomidine 0.25 μg/kg plus flurbiprofen axetil 50 mg (group DF),normal saline (group C),respectively.MAP,HR were recorded before extubation (T0 ),extubation (T1 ),5 mins after extubation (T2 ).The recovery time,extubation time,Riker sedation-agitation score(RSAS)be-fore extubation and Ramsay sedation score 5 min after extubation were observed.Results Compared with T0 ,MAP,HR at T1 ,T2 in group C and group F were significantly increased (P <0.05 or P <0.01),MAP,HR at T1 ,T2 in group D and group DF were significantly lower than those in group C (P <0.01 ).The recovery time,extubation time in group D were significantly longer than those in group C,group F and group DF(P <0.05).Ramsay scores in group D was significantly higher than other groups(P <0.05).The incidence of agitation in group D and group DF were significantly lower than those in group C(P <0.05 or P <0.01).Conclusion Dexmedetomidine 0.25 μg/kg plus flurbi-profen axetil 50 mg can effectively prevent agitation and reduce extubation cardiovascular reaction dur-ing recovery period,without the disadvantage of prolonging the recovery and extubation time.

Objective To explore the relationship between the expression of pS2 protein and the clinicopathological parameters in breast cancer and to evaluate the value of pS2 as a prognostic factor for breast carcinoma and a predictive factor for response to endocrine therapy. Methods Expression levels of pS2 protein, estrogen receptor (ER) and progestogen receptor (PR) in tissues from 75 cases of breast cancer were detected by immunohistochemical assay. The relationship of pS2 protein expression with patient age, menopausal status, tumor size, metastasis to the axillary lymph nodes, pathological types, ER and PR was analyzed. Results pS2 protein, expressed in 33.3% of breast carcinomas, was correlated with patient age and pathological types, but was not correlated with tumor size. The positive rate of pS2 protein before menopause was higher than that after menopause, but no significant difference was found. Similarly, higher positive rate of pS2 protein was found in patients with metastasis to axillary lymph nodes than those without, but no significant difference was found. pS2 expression was correlated with ER and PR, but they were not perfectly consistent. Conclusion In breast cancer, pS2 protein expression, associated with some clinical factors and pathological features, is a good prognostic factor. pS2 expression may be a reasonable index for endocrinotherapy of breast carcinoma, but the detection of pS2 protein can not be employed in place of the detection of ER and PR.

Objective To investigate the effect of music therapy on anxiety and depression in patients with spinal cord injury. Methods Forty anxious and depressive patients with spinal cord injury were selected as the control group from December 2012 to October 2013, and another 40 anxious and depressive patients with spinal cord injury as the observation group from November 2013 to September 2014. Patients in the observation group were given the following metheds after admiting for 3 days besides psychological nursing; music therapy once a day, 1 hours one time for 4 weeks. The two groups were compared in terms of the scores by Hamilton anxiety scale (HAMA) scores and Hamilton depression scale (HAMD) before and after treatment. Results There were insignificant differences in HAMA scores and HAMD scores between the two groups before music therapy (P>0.05), but the scores by HAMA and HAMD in the observation group were both significantly lower than those in the control group. Conclusion Music therapy can alleviate anxiety and depression of patients with spinal cord injury, and promote the functional recovery.

Adolescent ; Adult ; Aged ; Child ; Endoscopy ; Female ; Humans ; Intraoperative Period ; Middle Aged ; Otorhinolaryngologic Surgical Procedures ; methods ; Recovery of Function ; Smell ; Young Adult

Objective To calculate the incidence of chromosomal abnormalities at second trimester in women who were 35 or older at their expected date of birth.Methods The amniocentesis and karyotyping results in Peking Union Medical College Hospital from January 1st,2001 to June 30th,2011 were retrospectively analyzed.The only indication for amniocentesis in these group of woman was advanced maternal age.A total of 6584 cases Were included in this study and were divided into two groups according to maternal age,ie.35-39 and ≥40 year old group.The incidences of fetal 47,+ 21,47,+ 18 and sex aneuploidies were calculated and compared between two groups by Chi-square test.Results Altogether,121 cases were diagnosed to be abnormal chromosome,and the overall incidence was 18.38‰ (121/6584).The abnormal karyotypes included 111 cases of aneuploidies (mosaicism included) and 10 cases of structural abnormalities.The aneuploidies included 59 cases of 47,+21 (8.96‰,59/6584),25 cases of 47,+18 (3.80‰,25/6584),2 cases of 47,+13 (0.30‰,2/6584) and 25 cases of sex aneuploidies (3.80‰,25/6584).Fetal 47,+21 was the most frequent chromosomal abnormality,accounting for 53.15％ (59/111) of all aneuploidies.The incidence of fetal 47,+21 was significantly higher in ≥40 year-old group than that of 35-39 year old group[13.99‰(16/1144) vs 7.90‰(43/5440),x2=3.937,P=0.047].There were no statistical differences of the incidences of fetal 47,+ 18 and sex aneuploidies between the two groups.Conclusions The main fetal chromosomal abnormalities in women aged 35 and older are the aneuploidies of chromosome 21,18,13 and sex chromosomes.The incidence of fetal 47,+21 is significantly increased in the women aged 40 years and older.So prenatal screening should be provided first to women at 35-39 years of age and amniocentesis should be the first choice of prenatal diagnosis for women over 40 years old.

Objective To evaluate the clinical significance of thyroid stimulating hormone (TSH) and thyroid autoantibodies (anti-TGAb and anti-TPOAb) in cord blood of infants of mothers complicated with hypothyroidism and the influencing factors of neonatal thyroid function. Methods Clinical data of 67 singleton pregnant women complicated with hypothyroidism in Peking Union Medical College Hospital were analyzed retrospectively. Thyroid function and its autoantibody levels in maternal, cord blood and neonatal serum at 5-7 d after birth were compared. Umbilical TSH level and its affecting factors were also investigated. The results of TSH was expressed as median (25th-75th percentile). Results (1) Umbilical TSH levels were elevated in 9. 0% (6/67) of all infants born to mothers complicated with hypothyroidism. (2) No correlation was found in TSH levels between cord blood and venous blood in neonates 5-7 d after birth. Umbilical TSH levels were significantly higher in infants born vaginally than in those born abdominally [10. 20(6. 10-12. 80) mU/L vs 5. 86(4.02-7.74) mU/L,P=0.001]. Higher umbilical TSH levels were also detected in those complicated with fetal distress and preterm birth compared with those withoutere [fetal distress: (10. 36(6. 61-13. 37) mU/L and 6. 89(4. 18-9. 70) mU/L, P = 0. 046; preterm birth: 8. 90(7. 60-10. 33) mU/L and 6.84(4.17-9. 80) mU/L,P=0. 046,0. 049)]. (3) The anti-TGAb levels in cord blood were positively correlated with that in neonatal serum at 5-7 d after birth (r=0. 960, P = 0. 000), and the same was true for anti-TGPOAb levels (r= 0. 975, P = 0. 000). Maternal thyroid autoantibody levels (anti-TGAb and anti-TPOAb) had significant effect on umbilical antibody levels (P = 0. 003 and 0. 000, respectively), but not on the neonatal TSH levels (P>0. 05). Conclusions Umbilical TSH levels are affected by many delivery factors which may limit its prediction role on congenital hypothyroidism. However, there is an increased risk of elevated umbilical TSH, anti-TGAb and anti-TPOAb levels among these patients which may increase the risk of congenital hypothyroidism. Further follow up of these infants is warranted.

Objective To investigate the effects of the new drug Nordy on malignant glioma cells induced tubule-like structure formation of endothelial cells in a three dimensional culture system with collagen coating.Methods The endothelial cells were divided into three groups.Group A were cultured on the DMEM media coated with collagen;group B were cultured in 2:1(V/V) DMEM to the supernatant of malignant glioma cell culture;group C,based on the group B,were added with Nordy of different concentrations.The cell cycle and apoptosis of ECV304 cells were determined and the process of tubular formation were observed.Moreover,the parameters such as the forming curves,outer and inner diameter,wall thickness and length of the tubules were measured and compared between groups B and C.Results The cell cycle of endothelial cells were restrained,and some cells were induced to apoptosis.The tubule-like structure formation was inhibited,and the tubules in group B appeared earlier,larger in diameter and longer in length than group C.Conclusion The tubular formation induced by malignant glioma cells could be restrained by the new drug Nordy.