Objective To reduce information loss in the refining processing of photoplethysmographic (PPG) pulse waveform. Method A novel PPG pulse waveform refining processing technique, named truncation/extrapolation technique was proposed. The key point of the proposed technique was to preserve the shape pattern of the most significant parts of the waveform at the expense of the distortion of the insignificant part. Thus, as compared with the conventional time-normalization method, the proposed technique ensured the accumulation coherent of the signal energy could be. Result Waveform analysis and comparison of experimental data between two methods verified the effectiveness of the proposed method in maintaining the most significant time domain features of the pulse waveform over a relatively wide range of the heart rate change, especially the shape and width of the dominant peak and the position of the dicrotic notch of the first derivative of PPG(dPPG). Conclusion The proposed method reduces the information loss in PPG pulse waveform refining processing, thus helps further processing of the PPG signal using some advanced signal processing techniques, such as waveform modeling and pattern recognition.

Cryoprecipitation is one of the most important blood components.With the deeper study about cryoprecipitation,it has been used in clinics more and more extensively.This articles reviewed the new development of cryoprecipitation in clinical uses.

Objective To study the effects of phase change material (cooling agent) on the quality of blood after long-distant transportation.Methods A total of 12 bags, packed with pre-chilled phase change materials, were put into regular blood transportation kit, and the new blood transportation kit, which was developed by our hospital. In each kit there were 54 U of red blood cell suspensions. The temperatures in the kit were recorded during the transportation at different times.Results The temperature in the kit was kept between 1—7℃ during the 72h transportation. The temperature met the needs of red blood cell transportation.Conclusion Phase change material could be used as an effective cooling agent for long distance blood transportation.

Objective To investigate the relationship between serum 1 ,25‐hydroxy vitamin D3 concentration level and children with bronchial asthma .Methods 222 children with bronchial asthma treated in paediatrics of Zhongshan Boai hospital from 2012 Jan 2014 Dec were chosen as asthma group(A) ,while 223 cases of healthy children were selected as control group(C) .Electro‐chemical luminescence method was used to detect the serum level ,and its effect on asthma was disscussed .Results The concen‐tration of serum 1 ,25‐hydroxy vitamin D3 of asthma group (86 .30 ± 27 .70 nmol/L) was low than the control group ( 93 .39 ± 27 . 15nmol/)L .Serum levels of asthma group in different age groups (A1 ,A2 and A3) were 106 .87 ± 28 .28 nmol/L ,88 .98 ± 20 .65 nmol/L ,68 .38 ± 19 .23 nmol/L respectively ,While those of control groups were (110 .00 ± 22 .30) nmol/L ,(87 .36 ± 24 .42) nmol/L ,(21 .23 ± 16 .42) nmol/L respectively .Male and female serum level in asthma group were (88 .26 ± 27 .73)nmol/L and (81 .94 ± 26 .91)nmol/L respectively .While those of control group were(91 .91 ± 26 .57)nmol/L and (85 .69 ± 28 .04) nmol/L re‐spectively .Conclusion There is significant correlation between serum 1 ,25‐hydroxy vitamin D3 level and children with bronchial asthma .,yet no correlation between serum level and gender .1 ,25‐hydroxy vitamin D3 duficiency is one of the risk factors of chil‐dren asthma .

Objective To explore the effects of ultraviolet irradiation on platelet. Methods Three assays were been developed.These include platelet count,release of alpha granule membrane protein 140(GMP-140)and morphology of platelet by electron micrograph in those samples before and after ultraviolet irradiation.Results Compared with the nonirradiated controls,the irradiated units showed significant changes including decrease of platelet count,increase of release of GMP-140 and platelet lesion in morphology.Conclusion Ultraviolet irradiation may have activation and lesion effect in platelet.

Objective To test the performance of field blood transportation vehicle from the aspects of heat preservation,refrigeration and oil consumption.Methods Two vehicles without power supply were stowed with maximum load,one with red cell suspension,and the other with frozen plasma.With the environmental temperature higher than 35 ℃ or lower than 25 ℃,related time lengths were detected respectively.Results The holding time of the vehicles was from 1.5 h to 7 h,refrigerating time was from 15 min to 138 min,and a full tank of gasoline was exhausted around 5～7 hours.Conclusion During line-haul,field blood transportation vehicle has to be equipped with additional cooling agents,in case the refrigeration equipment out of work for the reason of gasoline exhaustion.

Objective Irregular antibody screening and identification are very important to reduce hemolytic reaction.The dis-tribution of irregular antibodies of blood group was analyzed in patients, to provide evidence for using Micro column gel test to detect and identify irregular antibodies transfusion safety. Methods Micro column gel anti-globulin technique was used to screen the irregular an-tibodies and cross-matching in 29770 patients.If the serum had irregular antibodies, the spectrum cells were used to identify the specific-ity of irregular antibodies. Results Among 29 770 patients, 120 cases had irregular antibodies of blood group.The positive rate was 0.4%.The irregular antibodies include Rh system(n=67),MNS system (n=7), Lewis system(n=5),cases of Kidd system(n=3) and undefined specified antibodies(n=38).Among the 120 antibody-positive patients, 70 were female patients and 50 were male patients. Further study showed that the pregnancy history was to the main factor causing irregular antibodies in women (46 positive cases among 70 female patients) . Conclusion The study showed that some patients had irregular antibodies of blood group, especially female patients with pregnancy history.These results suggested that irregular antibody screening and cross-matching by micro column gel method in pa-tients before transfusion had great significance for ensuring the blood transfusion safety.

Objective To evaluate the influence of blood transfusion or pregnancy on blood cross-matching results.Methods 1 000 specimens with blood transfusion or pregnancy history were collected and performed the blood group typing,blood cross matching,irregular antibody screening and identification by the micro-gel method with the specimens of first time transfuse blood and without pregnancy history as the control.The inconsistency rate of positive negative blood grouping,unqualified rate of blood cross matching and the positive rate of irregular antibody were compared between the two groups by the t-test.The causes influen-cing the blood cross-matching results were analyzed.Results The inconsistency rate of blood cross-matching in the blood transfu-sion or pregnancy group was significantly higher than that in the control group(P <0.05);the inconsistency rate of positive nega-tive blood grouping was similar in the two groups(P >0.05);the positive rate of irregular antibody screening in the blood transfu-sion or pregnancy group was also significantly higher than that in the control group(P <0.05),indicating that the main causes in-fluencing blood cross matching was the existence of irregular antibodies in the patients in vivo.Conclusion Blood transfusion or pregnancy may stimulate the body to produce the irregular antibodies.Therefore the irregular antibody screening should be per-formed before blood transfusion and blood without corresponding antigen will be selected for transfusion according to the antibody identification results for ensuring safe and effective blood transfusion.

Background It has not been reported that if the visual cortex M receptor changed during the development of myopia and how it changed if given acupuncture treatment.Objective The aim of this study was to observe the effect of electroacupuncture stimulation on the expression of acetylcholine receptors M1 (AchRM1) in visual cortex of guinea with lens-induced myopia (LIM).Methods Forty-eight three-week-old healthy guinea pigs were randomized into the normal control group,the LIM model group and the LIM electroacupuncture group.The right eyes of the guinea pigs were selected as the experimental eyes.LIM was created by monocularly wearing of-10 D lens for 4 weeks in the right eyes in the LIM model group and LIM electroacupuncture group,and then the acupuncture at the temple and hegu point was performed for 30 minutes per day for consequent 4 weeks,in the LIM electroacupuncture group.The fellow eyes of the guinea pigs were used as the self-control eyes.The refractive power and axial length were examined with retinoscopy and A-type sonography before and 4 weeks after modeling,respectively.The animals were sacrificed by excessive anesthesia at the fourth week after acupuncture and visual vertex tissue was obtained.The expression of M1 receptor mRNA in visual vertex was detected by fluorescence quantitative PCR,and the content of M1 receptor protein in visual vertex was assyed by ELISA.The study protocal was approved by Animal Ethic Committee of Shandong University of Traditional Chinese Medicine,and the use and care complied with Statement of the Association for Research in Vision and Ophthalmology.Results At the fourth week after modeling,the mean diopters were (-3.24±0.28) D and (-3.30±0.45) D in the LIM model group and the LIM eleetroacupuncture group,which were significantly higher than (0.83 ±0.86)D in the normal control group (both at P=0.000),and there was no significant difference in the diopter between the LIM model group and the LIM electroacupuncture group (t =0.200,P =0.659).The mean axial lengths were (8.67 ±0.14) mm and (8.60±0.06) mm in the LIM model group and the LIM electroacupuncture group,which were considerably increased in comparison with (8.33±0.08)mm in the normal control group (both at P＜0.05).The relative expression levels of AchRM1 mRNA in visual cortex were 0.79±0.18,1.36±0.23 and 1.13±0.13 in the normal control group,LIM model group and LIM electroacupuncture group,and the relative expression level of AchRM1 mRNA in the LIM electroacupuncture group was significantly higher than that of the normal control group and lower than that of the LIM model group (both at P＜0.05).In addition,the contents of AchRM1 receptor protein in the visual cortex were 248.00±33.31,455.17±42.40 and 396.17±47.57 in the normal control group,LIM model group and the LIM electroacupuncture group,with a similar pattern among the groups (both at P＜0.05).Conclusions A electroacupuncture stimmulation do not affect the myopic diopter and axial length in LIM model.The AchRM1 and AchRM1 receptor in the visual cortex up-regulate in LIM eyes,infering that electroacupuncture stimmulation can improve vision by decreasing the level of AchRM1 receptor in visual cortex in LIM eyes in guinea pigs.

OBJECTIVE To compare two different observed data from Nov 2003 to Apr 2004 and from Jan 2005 to Dec 2005 on hospital-acquired infection in our hospital and put forward the counterned measures.METHODS The bacteria samples from clinic were cultured.RESULTS The Pseudomonas aerugonosa,Acinetobacter baumannii and Escherichia coli were common strains of bacteria according to the two years data in our hospital.They were 30.35%,18.41% and 8.46% vs 19.74%,17.11% and 14.04%,respectively.The P.aerugonosa infective constituent ratio was decreased(30.35% vs 19.74%,P