Objective To compare the analgesia effect of lidocaine carbonate combined with intravenous flurbiprofen axetil with intravenous flurbiprofen axetil on intrapelvic irrigation and incision infiltration after gynecological laparoscopy.Methods Seventy-five patients scheduled for gynecological laparoscopy under general anesthesia were divided into 3 groups by random number table method with 25 cases each.Patients in control group 1 receivcd intravenous flurbiprofen axetil 100 mg after surgery;patients in control group 2 received intrapelvic irrigation with 0.35％ lidocaine carbonate 100 ml,and incision infiltration with 0.87％ lidocaine carbonate 10 ml respectively after surgery;patients in observation group received the combination of control group 1 and control group 2.The visual analogue scores (VAS) at 1,4,8,12 and 24 h after surgery,time of passage of gas by anus and untoward reaction were recorded.Results One case in control group 1 and 1 case in observation group withdrew from the study.The VAS at 1,4,8,12 and 24 h in observation group were (9.5 ± 7.9),(14.9 ± 8.7),(17.2 ± 10.3),(12.2 ± 7.7),(5.3 ± 3.8) mm,in control group 1 were (39.2 ± 15.0),(33.4 ± 13.0),(36.2 ± 12.8),(35.8 ± 12.7),(10.6 ± 4.2) mm,and in control group 2 were (26.6 ± 13.0),(30.2 ± 12.0),(33.3 ± 13.1),(30.4 ±9.8),(9.8 ±4.7) mm.And there were statistical differences between observation group and control group 1,2 (P ＜ 0.05).There were no statistical differences in time of passage of gas by anus and untoward reaction incidence in the 3 groups (P ＞ 0.05).Conclusion Intrapelvic irrigation and incision infiltration with lidocaine carbonate and intravenous flurbiprofen axetil compared with intravenous flurbiprofen axetil alone after gynecological laparoscopy can significantly reduce the pain intensity and analgesia requirement,without increasing the untoward reaction incidence.

Oyster complete nutritional tablet was used in treating 40 patients with yin -deficiency syndrome due to metaphase -advanced stage carcinnoma. The results showed that the general symptoms of patients were improved obviously, the hemogram was gone up and some of the immunologic indices was ameliorated. The tablet contributed to relaxing the chest,regulating vital energy,clearing away lung -heat and eliminating sputum,showing the efficacy for moisturizeing dryness by nourishing yin.

Objective To evaluate the diagnostic value of MSCT in peripheral pulmonary neuroendocrine carcinoma.Methods CT findings of 42 peripheral pulmonary neuroendocrine carcinoma confirmed pathologically were compared among different pathological types.Results As for lymphatic metastases,small cell carcinoma was more commom than other pathological types(P＜0.05).Most of peripheral pulmonary neuroendocrine carcinoma had homogeneous density(29 cases) and was lobulated(30 cases).The specula sign,vessel convergence sign,pleural indentation sign and obstructive pneumonia in this group were rare(8 cases,4 cases,7 cases,8 cases respectively).Most lesions had enhancement lightly.CT values of the lesions and the enhanced degree were similar in different pathological types and there was no difference among them(P＞0.05).Conclusion msct is of definitive value in diagnosis or differentitating diagnosis in the different pathological types of peripheral pulmonary neuroendocrine carcinoma.

Objective To identify a detailed biological characterization of mesenchymal stem cells (MSCs) isolated from hu‐man umbilical cord(UC) tissue regarding their morphology ,immunophenotype ,purity and proliferative capacity and establish a rea‐sonably cultured and amplified system .Methods After stripping off arteries and veins ,the remaining parts of umbilical cord were cut into 1 mm3 small sections and cultured with DMEM/F12 containing 10% fetal bovine serum .Adhere cells were obtained and the morphology of the cells was observed under inverted phase contrast microscope .The growth curves of them were drawn by CCK‐8 and the cell cycle and surface antigens (CD29 ,CD73 ,CD90 ,CD105 ,CD31 ,CD14 ,CD34 ,CD45 ,CD11b ,HLA‐DR) were detected by flow cytometry .Results Seven to ten days after primary culture ,adhere cells came out of fragments .The MSCs harvested were a high purity and mainly presented as a fibroblast‐like morphology .UC‐MSCs had a strong ability of proliferation through the cell growth curve .The special surface antigens CD29 ,CD73 ,CD90 ,CD105 were positive expression ,while CD31 ,CD14 ,CD34 ,CD45 , CD11b ,HLA‐DR were negative .More than 80% cells of MSCs were found at G0/G1 phase .Conclusion Human UC‐MSCs could be cultured and proliferated in vitro .

Objective To determine the aberrant methylation status of TFPI-2,FOXE1 and NPTX2 gene promoter region in bile from cholangiocarcinoma patients and the diagnostic value for cholangiocarcinoma. Methods Methylation—specific PCR(MSP)was used to detect the promoter methylation status of TFPI-2, FOXE1 and NPTX2 genes in bile from 29 patients with cholangiocarcinoma and 27 patients with choledocholi-thiasis.The sensitivity and specificity of combined methylation of 3 promotors and brush cytology were analyzed. Results There was significant difference between the methylation rate of NPTX2,FOXE1 in choledocholithiasis group and cholangiocarcinoma group(75. 86% VS 25. 93%,χ2 = 13. 964,P<0. 05;44. 82%VS 14. 81%,χ2 = 4. 622,P<0. 05).There was no significant difference between the methylation rate of TFPI-2 in choledocholithiasis group and cholangiocarcinoma group(17. 24%VS 18. 52%,χ2 = 0. 016,P>0. 05).Positive rate was 34. 48% with endoscopic retrograde brush cytolog,and 86. 20% with promoter methylation of FOXE1 combined with NPTX2 genes,and there was significance difference in the positive rates between the two methods(χ2 = 14. 122,P< 0. 05). Conclusion The methylation rates of NPTX2,FOXE1 were significantly higher in choledocholithiasis group than those in the cholangiocarcinoma group. Detection of comethylation rate of the FOXE1 and NPTX2 genes could improve diagnostic sensitivity of cholangiocarcinoma.

Objective To investigate the protein expressions of E-cadherin and ?-,?-catenin in breast cancers and their lymph-node metastatic tumors to evaluate their possible role in breast cancer invasion and metastasis. Methods High sensitive S-P immunohistochemical method was used to detect the protein expressions of E-cadherin and ?-,?-catenin in 60 breast cancers(31cases with lymphatic metastasis) and their lymph-node metastatic tumors. Results The abnormal expression rates of the E-cadherin and ?-,?-catenin were 51 7%, 61 7%, 70% respectively in the 60 cases of breast cancers. The expression of E-cadherin was significantly associated with lymphatic metastasis(P

Objective To observe the clinical manifestations and the effect factors of thyroid-associated ophthalmopath (TAO) with optic neuropathy. Methods The clinical data of 62 cases (120 eyes) of TAO with optic neuropathy diagnosed in 1994-2001 were retrospectively analyzed. Results The incidence of TAO with optic neuropathy was 18.3% in all the simultaneous TAO inpatients, and was more frequently found in the male than in the female ones. The incidence of TAO with optic neuropathy was correlated with orbitono-increase, ocular myopathy, hypertension, hyperlipemia, diabetes, and cardiopathy (P0.05). In 62 patients with the methylprednisolone pulse therapy, the visual acuity improved in 33 (29 0%), improved while the treatment but decreased after stop treating in 29 (24.2%), and no changes in 58 (46.8%). In 63 eyes undergone orbital decompression with methylprednisolone pulse therapy, 59 (93.7%) had better visual acuity. Conclusion The incidence of TAO with optic neuropathy was correlated with orbitono-increase, ocular myopathy, hypertension, hyperlipemia, diabetes, and cardiopathy. Methylprednisolone pulse therapy and orbital decompression are the effective measures for TAO with optic neuropathy.

Objective To investigate the prevalence of H.Pylori infection and antibiotic resistance in patients with peptic ulcer.Methods A retrospective analysis of 429 endoscopy and H.Pylori culture results in the First People's Hospital of Hangzhou from 2012 to 2013 was conducted.The rate of H.Pylori infection in patients with peptic ulcer, and the resistance rates of H.Pylori to metronidazole, levofloxacin, clarithromycin,amoxicillin, gentamicin, furazolidone were analysed.Results The positive rate of H.Pylori in people with peptic ulcer was 53.1％ (228/429).The resistance rates to metronidazole, levofloxacin, clarithromycin,amoxicillin, gentamicin and furazolidone were 94.7％ (216/228), 18.9％ (43/228), 18.9％ (43/228), 0, 0 and 0.Double antibacterial resistant rates of metronidazole and clarithromycin, metronidazole and levofloxacin were 18.0％ (41/228) and 17.1％ (39/228).Triple antibacterial resistant percentage of levofloxacin + metronidazole + clarithromycin was 8.3％ (19/228).The resistance rate of clarithromycin in 43 patients who were also resistant to levofloxacin was 46.5％ (20/43), while the resistance rate of clarithromycin in 185 patients who were also sensitive to levofloxacin was 12.4％ (23/185)with significant difference(x2 =26.480,P =0.000).A positive association between the resistance to levofloxacin and to clarithromycin was found.Conclusion The H.Pylori infection is closely related to the occurrence of peptic ulcer.Without the basis of culture results in patients with H.Pylori positive, amoxicillin, gentamicin and furazolidone could be the first choices in clinical,because of their low resistance rates.Clarithromycin can be used as an alternative.

Objective To observe the intervention effect of Wuxing-Jiangu exercise for different syndrome types of elderly patients with chronic low back pain.Methods A total of 199 patients who met the criteria of chronic low back pain (CLBP) were divided into a Wuxing-Jiangu exercise group (86 cases), a control group (85 cases) and a health education group (28 cases). On the basis of health education, theWuxing-Jiangu exercise group was given Wuxing-Jiangu exercise; the control group was treated with the warm type frequency therapeutic instrument; health education group was only given health education guide. The changes of the score of CLBP patients before and after treatment with different syndrome types were observed.Results After 3 months of treatment, the score of the kidney deficiency syndrome and spleen deficiency syndrome in the Wuxing-Jiangu exercise group, the control group and the health education group had significant differences (H were12.554, 8.014, respectively;all P<0.01); After 3 months of treatment, the score of kidney deficiency syndrome and spleen deficiency syndrome in the Wuxing-Jiangu exercise group reduced than before the treatment (Z were -5.975,-5.025, respectively)and 1 month after the treatment(Z were -5.976,-5.026, respectively) (all P<0.01).Conclusion Wuxing-Jiangu exercise can reduce the score of the kidney deficiency syndrome and spleen deficiency syndrome and improve clinical syndrome.

Objective To compare the characteristics in initial sandplay of college students with high and low gregarious personality,to validate the psychological assessment function of the initial sandplay.Methods Used the 16-PF as the tool to selected the 56 students as the high gregarious group,and 36 students as the low gregarious group.Results On selection of toys,the proportions of high gregarious group were significantly higher than those in low gregarious group in total numbers,plants and daily necessities ((27.77 ± 11.01)vs (20.94 ± 8.76),(7.30 ±6.43)vs(3.25 ±2.85),(3.66 ±2.86)vs(2.25 ±2.57)),and significantly lower in religion toys (0.30 ± 0.47) vs (0.86 ± 1.09).On operation process,the proportion of starting with toys in high gregarious group was significantly higher than that in low gregarious group (94.6％ vs 25％).Their attitudes towards sand were random touch(28.6％ vs 5.6％) and indifference(62.5％ vs 41.7％).They used more grass(55.4％ vs 27.8％),more real characters(83.9％ vs 50％),fewer virtual characters(55.4％ vs 83.3％) and more dynamic changes in creation(14.3％ vs 0％),more applied by the social scene (39.3％ vs 0％).On the theme of sandplay,the high gregarious group was fewer appear by the empty,neglect,injury ((3.6％ vs 19.4％),(0％ vs 8.3％),(0％ vs 8.3％) respectively,P ＜ 0.05).Conclusion The results show that the high and low gregarious personality have significantly characteristics in initial sandplay.Sandplay is valuable to be a personality assessment tool.