Based on Belphi7.0, this paper designs a software of HIS for medicine warehouse management, which can provide timely dynamic information of the medicine. With the application of this software, required medicine can be supplied in time and the overstocking can be diminished, thus not only accelerates medicine circulation, but greatly promotes work and management efficiency. The combination of this software and HIS shows such advantages as quick response, comprehensive information, convenient installment, practical and safe in application, etc.

AIM： To estabish a HPLC assay for the determination of pirarubicin（Pir） in plasma.METHODS： Daunomycin（DM） was used as the internal standard.Plasma samples were extracted with CHCl3∶ CH3OH（2∶ 1） .0.4M NH4Cl buffer（pH=9.0） solution： CH2OH（1∶ 9） and the internal standard were added.Separation was carried out on a 250mm× 4.6mm（5μ m） phenomenex column with 0.04M KH2PO4（pH=3.0） ∶ CH3CN（68∶ 32） as mobile phase.Fluorescent detector was set at an excitation wavelength of 480nm and an emission wavelength of 550nm.RESULTS： The calibration curves for serum Pir was linear over the range of 5～1 000ng/ml（r=0.9 997） .The recovery of Pir was 95.3％ .The within-day and between-day variations were less than 5％ .T1/2β ， CLs， Vd and AUC of Pir were（12.8± 5.9） h， （128.3± 52.6） L/（m2· h） ， （1 754.3± 478.2） L/m2 and （428.7± 137.2） ng/（h· ml） ， respectively.CONCLUSION： The method is suited for monitoring blood concentration and pharmacokinetic study of Pir.

The situation of the ongoing fifth epidemic (beginning October,2016) of human infection with avian influenza A (H7N9) virus are more serious than the first four ones.As of March 8,2017,the fifth epidemic reported 40.00％ of the cumulative cases.Recently,the pathogenic study reported two new human infections in Guangdong with an influenza A(H7N9)virus strain for which gene sequencing analysis revealed mutations in the haemagglutinin (HA) gene that resulted in the insertion of basic amino acids at the cleavage site of this protein,known to confer increased pathogenicity in chickens.However,most strains show no obvious difference in pathogenic characteristics compared with those detected during the first four waves.Although epidemiological studies showed that 3 clusters with limited human-to-human transmission have been investigated during the current wave,there are no indications of sustained person-to-person spread.Based on epidemic analysis and risk assessment results,it's likely that human infections with H7N9 virus will continue to occur in China,but epidemiological and pathogenic analysis suggested that it's unlikely to have a continued transmission of this virus.Profound public health significance would be presented by strengthening etiology and epidemiology studies of avian influenza A(H7N9) virus.

BACKGROUND:Thyroid-stimulating hormone receptor is an autoantigen shared by thyroid follicuar cells and ocular orbital tissues,and cytokines plays an important role on the pathogenesis of Graves ophthalmopathy(GO) . It is still a puzzle that the relation of cytokines and thyroid-stimulating hormone receptor in the pathogenesis of GO. OBJECTIVE:To study the influence of various cytokines on the expression of thyroid-stimulating hormone receptor in the process of adipogenesis of in vitro cultured orbital adipose cells in GO patients. DESIGN,TIME AND SETTING:A single sample observation was carried out in the Central Laboratory,Department of Ophthalmology,Sun Yat-sen University(Guangzhou,Guangdong,Province) from September 2003 to March 2004. MATERIALS:Orbital adipose cells were obtained from eight serious GO patients undergoing orbital decompression. Recombinant human interleukin 2(IL-2) ,IL-4,IL-6,IL-10,and interferon ?(IFN-?) were purchased from PEORO TECH EC Company. METHODS:Orbital adipose cells were cultured and treated by groups. IL-2,IL-4,IL-6,IL-10 and IFN-? was added to the culture media in the experimental group,while no any cytokine was given in the untreated group,and orbital adipose cells were primarily cultured in the control group. The cells were all collected twelve days later. MAIN OUTCOME MEASURES:The expression of thyroid-stimulating hormone receptor was examined with reverse transcriptase-polymerase chain reaction,and the content of cyclic adenosine monophosphate was measured with simultaneous radioimmunoassay. The quantitation of adipose in cells was detected by staining intracytoplasmic lipids with oil red O. RESULTS:The expression of thyroid-stimulating hormone receptor was significantly higher in cells treated with IL-2,IL-4,IL-6 and IL-10 than that in the untreated cells and in the cells treated with IFN-?. Treatment of GO orbital adipose cells with IL-2,IL-4,IL-6 and IL-10 during differentiation resulted in significantly greater cyclic adenosine monophosphate production by different degrees. The highest production achieved in the treatment with IL-6,which was two times as many as control group. The cyclic adenosine monophosphate production was significantly lower in the cells treated with IFN-? than in the untreated cells. CONCLUSION:IL-6,IL-4 and IL-10 can upregulate the expression of thyroid-stimulating hormone receptor in the orbital adipose cells of GO patients,and stimulate adipose differentiation. Conversely,IFN-? can restrain this process.

Objective To evaluate a new model of anterior spinal cord injury (SCI) syndrome and to explore the pathophysiology of SCI. Methods Fifty-five Wistar rats were randomly divided into a sham-operated group ( 10 rats) and an experimental group (45 rats). A metal hook was fixed in front of the rats'abdomens to compress the ventral part of the spinal cord in the experimental group. According to the degree and time of compression,the rats in the experimental group were divided into light, moderate, heavy injury subgroups. The tilt board test and the Bosso-Beattie- Bresnahan (BBB) locomotor rating scale were used to assess the rats' behavior at the 1st , 7th andl4th days and after 4 and 8 weeks. The latency of somatosensory evoked potential (SEP) and motor evoked potential (MEP) were measured before and 8 weeks after the operation. Results After the operation the gradients in the tilt board test and BBB scores in the experimental subgroups were all significantly lower than in the sham-operated group. There were also significant differences among the 3 severity subgroups. Eight weeks after the operation the average MEP latencies in the experimental subgroups were significantly longer than in the sham-operated group, and there were also significant differences among the 3 severity sub-groups. MEP in the heavy injury subgroup was significantly longer compared with the sham-operated group. Conclusions Obvious behavioral and neuroelectrophysiological changes were observed in the injured rats. Models of different severity could be prepared and reproduced to simulate clinical SCI.

In order to understand the alcohol's toxicity to the quantitative alternations of synapses in mouse visual cortex, the expression of synaptophysin after prenatal alcohol exposure was investigated. In present study, the experimental mice at P0, P7, P14 and P30 were grouped, as control, 2 g x kg(-1) alcohol treatment and 4 g x kg(-1) alcohol treatment. The pre-synaptic elements which were used to represent synapses were marked with synaptophysin (a synaptic vesicle associated protein) by immunocytochemistry technique. The synaptophysin positive boutons in layer VI of visual cortex were imaged under laser confocal microscope. With stereological methods, the number cal density of synapse in visual cortex was calculated in different groups at various ages. Moreover, Western blotting was carried out to detect the expression of synaptophysin in visual cortex. The results showed that prenatal alcohol exposure could cause synaptic loss with long-term effect and in a dose dependent manner. For instance, there were significant difference among the different treatment groups of P0, P14 and P30 as well (P < 0.05). Western blotting supported the results of immunofluorescent labeling. In conclusion, prenatal alcohol exposure can induce the synaptic loss dose dependently and with long-term effect. Our findings implicate that the synaptic loss with long-term effect in CNS probably contributes to the lifelong mental retardation and memorial lowliness associated with childhood FAS.

Objective To observe the effect of electroacupuncture and body-weight supported treadmill training in the treatment of spinal cord injury (SCI) after fractures of the thoracolumbar segment of the spine.Methods Forty-three patients with SCI after fractures of the thoracolumbar segment of the spine were randomly divided into a treatment group (22 cases) and a control group (21 cases). The patients in the treatment group were treated with electroacupuncture and body-weight supported treadmill training, while those in the control group were treated mainly with drugs. Patients were scored with the American Spinal Injury Association (ASIA) assessment and the modified Barthel index (MBI) initially and after 6 months. Results The ASIA and MBI scores of all patients were significantly higher after 6 months than before treatment, but those in the treatment group were significantly higher than those in the control group. Excellent and good ratings were applied to 77% of the treatment group, while only 48% of the control group, a statistically significant difference. Conclusions Electroacupuncture and body-weight supported treadmill training can effectively improve ability in the activities of daily living, sensation and locomotor abilities after thoracolumbar vertebral fractures.

Magnetic resonance (MR) images can be used to detect lesions in the brains of patients with multiple sclerosis (MS). An automatic method is presented for segmentation of MS lesions using multispectral MR images in this paper. Firstly, a Pd-w image is subtracted from its corresponding T1-w images to get an image in which the cerebral spinal fluid (CSF) is enhanced. Secondly, based on kernel fuzzy c-means clustering (KFCM) algorithm, the enhanced image and the corresponding T2-w image are segmented respectively to extract the CSF region and the CSF-MS lesions combinatoin region. A raw MS lesions image is obtained by subtracting the CSF region from CSF-MS region. Thirdly, based on applying median filter and thresholding to the raw image, the MS lesions were detected finally. Results were tested on BrainWeb images and evaluated with Dice similarity coefficient (DSC), sensitivity (Sens), specificity (Spec) and accuracy (Acc). The testing results were satisfactory.
Algorithms ; Brain ; pathology ; Humans ; Image Enhancement ; Magnetic Resonance Imaging ; Multiple Sclerosis ; diagnosis ; Sensitivity and Specificity

Objective To observe the effectiveness of comprehensive rehabilitation for neurogenic bladder of patients with spinal cord injury and prevent urinary tract infection (UTI). Methods Fifty-seven patients with spinal cord injury were randomly divided into two groups: treatment group (29 cases) and control group (28 cases). The patients in treatment group were treated with comprehensive rehabilitation,while those in control group were treated mainly with catheterization and TDP equipment. Scored with Barthel index and the rate of UTI. Results It was shown that the improvement of Barthel index after treatment [(8.93 ± 6.27) scores] and the rate of UTI [13.79%(4/29)] were significantly better than those in control group[(5.16 ± 6.82) scores,67.86%(19/28)], which had significant differences (P＜ 0.05). Conclusion Comprehensive rehabilitation can reconstruct bladder function and prevent UTI of patients with spinal cord injury effectively.

Objective To assess the renal protective effects of mycophenolate mofetil(MMF) in diabetic model rats and to explore its mechanism. Methods SD rats were divided randomly into there groups: control group,diabetic model group and diabetic group treated with MMF. In 12 weeks, blood glucose(BG), blood urea nitrogen(BUN), serum creatinine(Scr), index number of kidney hypertrophy(kidey weight to body weight, KW/BW),creatinine clearance(Ccr) and 24- hour urinary protein (24Upro) were detected. Kidney tissure were examined by microscopy. The protein exprssion of intercellular adhesion molecule-1 (ICAM-1) and proliferating cell nuclear antigen(PCNA) in renal tissure were determined by immunohistochemical technique. The mRNA expression of ICAM-1 in kidney tissure were semi-quantitatively determined with reverse transcription-polymerase chain reaction(RT-PCR). Results Compared with control group, BG、KW/BW、24Upro、BUN、Scr and Ccr were significantly increased in diabetic model rats(P