Epidermal growth factor (EGF) is a multifunctional growth factor,which is widely expressed in most ocular tissues including cornea,retina and sclera.It may regulate the growth and proliferation of various ocular cells with the interaction of epidermal growth factor receptor in vitro or in vivo.This article reviews the role of EGF in pathological and physiological processes of ocular diseases,including corneal injury,retinal proliferative diseases,myopia,pterygium,glaucoma,cataract,and eye tumors.

OBJECTIVE:To establish the HS-GC method for the content determination of menthol in Qiangli Pipa syrup,and the content of menthol in 56 batches of Qiangli Pipa syrup commercially available was determined. METHODS:HS-GC was per-formed on the column of Agilent HP-INNOWAX,column temperature was 130 ℃(maintaining 7 min),FID was used as detector, inlet temperature was 200 ℃,detector temperature was 250 ℃,carrier gas was high-purity nitrogen,flow rate was 3 ml/min,split ratio was 10∶1 and the injection volume was 1 000 μl. RESULTS:The linear range of menthol was 0.007 07-0.141 40 mg/ml(r=0.999 1);RSDs of precision,reproducibility and stability tests were no more than 3.37%;average recovery was 94.3%-99.6%(RSD=1.86%,n=6). There was significant difference in the contents of menthol in 56 batches Qiangli Pipa syrup. CONCLU-SIONS:The method is simple,sensitive,accurate and reliable,and can be used for content determination of menthol in Qiangli Pi-pa syrup. The sampling results show it is necessary to update the detecting items for the content of Qiangli pipa loquat and strength-en the quality control of it.

Objective To evaluate the effects of personality on the development of postoperative cognitive dysfunction (POCD) in elderly patients undergoing gastrointestinal surgery.Methods Fifty-eight elderly patients,aged 65-70 yr,of American Society of Anesthesiologists physical status Ⅱ or Ⅲ,with Eysenck Personality Questionnaire (EPQ) P rating scale score ＜50,scheduled for elective gastrointestinal surgery under general anesthesia,were enrolled in the study.Personality was assessed by using EPQ at 1 day before surgery.By using a 2 × 2 factorial design,2 empirical factors introversion or extroversion (factor A) and neuroticism (factor B) were used.A1 and A2 were introversion (EPQ E rating scale score＜50) and extroversion (EPQ E rating scale score ≥ 50),respectively.B1 and B2 were emotional stability (EPQ N rating scale score＜50) and emotional instability (EPQ N rating scale score ≥ 50),respectively.The patients were divided into 4 groups:A1B1 group (n=8),A1B2 group (n=10),A2B1 group (n=26),and A2B2 group (n =14).Cognitive function was assessed at 7 days after surgery,and the development of POCD was recorded.Results The incidence of POCD was 0,0,15％ and 57％ in A1B1,A1B2,A2B1and A2 B2 groups,respectively,the incidence of POCD was significantly higher in group A2B1 than in A1B1and A1 B2 groups,and the incidence of POCD was significantly higher in group A2B2 than in the other three groups (P＜0.05).Factor A and factor B produced effects on the development of POCD (P＜0.01),and an interaction between them was found (P＜0.01).Conclusion The incidence of POCD is higher for the non-mentation patients who are extroverted and emotionally unstable.

Objective To establish mice model that induces mucosal immunity by orally infected tachyzoites of Toxoplasma gondii. Methods Respectively, 5?103, 5?104, 5?105, 5?106 tachyzoites of RH strain were inoculated to BALB/c mice by stomach delivery. The control group was given PBS solution. Symptoms and pathological changes of mice were observed. Secretory im-munoglobulin A (SIgA) was assayed. The lymphocytes from Peyer's patches (PP) and intraepithe-lial lymphocyte (IEL) were observed and the changes of CD4+ and CD8+ T cells assayed by SABC immunohistochemistry. Results Inoculation of 5?104 tachyzoites of Toxoplasma gondii caused symptoms and pathological changes in mice. The titre of SIgA increased in intestine, and CD4+ T subset of the mucosal inductive sites and CD8+ T subset of the mucosal effectors' sites increased. Conclusion Mucosal immunity may be induced by oral infection of 5?104 tachyzoites of T. gondii RH strain in BALB/c mice.

Objective To culture preadipocytes in vitro and to study the cell compatibility of PLGA scaffolds,collagen scarfolds and hyaluronic acid-based scaffolds and tO choose the optimal seeding method.Methods The preadipocytes from human abdominal adipose tissue were isolated and cultured in enzyme-digesting method.The generation of human preadipocytes was planted on PLGA scaffolds,collagen scaffolds and hyaluronic acid-based scaffolds.and the cell compatibility was observed by MTT method.The seeding efficiency of human preadipocytes on scaffolds.human preadipocytes were seeded to hyaluronic acid-based scaffolds by static culture and stirred culture.Results Compared compatibility of preadipocyte with three different scaffolds,there was great difference between hyaluronic acid-based scaffolds and PLGA scaffolds.Difference also existed between hyaluronic acid-based scaffolds and collagen scaffolds that were different from PLGA scaffolds.Among them,hyaluronic acid-based scaffolds was the best.Conclusion Hyaluronic acid iS a better scaffolds material for adipose tissue engineering compared with PLGA and collagen.The seeding efficiency of stirred culture is higher than static culture,which is an optimal method for cell seeding tO 3-D scaffolds.

Objective To observe the near future curative effect and toxicity of the combination of weekly dose of cisplatin in the treatment of elderly patients with esophageal cancer intensity-modulated radiotherapy observa-tion.Methods 60 patients with locally advanced esophageal cancer patients aged by method randomly were divided into synchronous chemotherapy group( CRT group) and 30 cases of radiotherapy alone group( RT group) 30 cases,the two groups using 6MV-X ray intensity modulated radiotherapy,radiotherapy dose:DT 60 Gy/30/6 week.The CRT treated group was given cisplatin 20-25mg/m2 in the radiotherapy of second day,1 times per week,a total of 6 times. Results Radiotherapy and chemotherapy, radiotherapy group recent effective rate were 80.0% and 63.3%, the difference was statistically significant(χ2 =5.934,P<0.05),P< 0.05).The main toxicity was myelosuppres-sion,radiation esophagitis,basically I and II,no statistically significant difference between the two groups(P>0.05). Conclusion Intensity modulated radiotherapy combined with weekly dose of cisplatin in the treatment of elderly patients with esophageal cancer significant short-term effect,less adverse reactions,patient tolerance is good,which is worth the clinical promotion.

Objective To study the influence of CRT brightness on image reading. Methods Both physical testing and compared viewing were used in the study. (1) Setting the CRT brightness at high and low grade (the blank brightness of CRT was 112 cd/m 2 and 82 cd/m 2, respectively). (2) 21 films with the density ranging from 0.27 to 2.71 were digitized by a scanner and displayed on the CRT. The brightness of CRT that varied with film density was measured by a bright meter, then the relation chart between CRT brightness and the film density was drawn. (3) Thirteen films on which there were many small and low contrast signals were viewed on CRT by three doctors. The tested data were calculated by statistics (? 2 test, the difference was significantly marked when P 0.05), but the signals viewed in the lung were 87 and 29, and the difference was significantly marked (? 2=53.73, P

Objective To investigate the infection situation of intestinal nematodes and knowledge about the prevention and control of intestinal nematodiasis,so as to explore the effective control measures in Jingjiang City. Methods The towns where more floating people lived were randomly selected and the infection situation of intestinal nematodes was investigated with Kato-Katz method,and the residents'awareness of the prevention and control of nematodiasis was surveyed with questionnaires. Re-sults From 2013 to 2015,totally 4555 local residents and 2278 floating people were investigated in Jingjiang City. The infec-tion rate of intestinal nematodes was 0.29%(13 cases)in the local people,while the rate was 0.75%(17 cases)in the floating people,and the difference was significant(χ2=7.380,P<0.01). The differences of the intestinal nematode infection rates be-tween sexes in both local residents and floating people were not significant(χ2=0.010,0.048,both P>0.05). The awareness rate of intestinal nematodiasis prevention and control of the local residents was significantly higher than that of the floating peo-ple(χ2 =9.649-164.533,all P<0.01). Conclusion The floating people is the focus of intestinal nematodiasis control,and the health education of ancylostomiasis control should be strengthened in Jingjiang City.

Objective To study the relationship of cataract with lens epithelial cell apoptosis and autophagy by observing the ultrastructure of age-related and diabetic cataract lens epithelial cells.Methods Transmission electron microscope was used to observe the apoptotic and autophagic morphology of the lens epithelial cells in 5 cases with age-related and diabetic cataract respectively,and in 2 cases with normal lens.Results The apoptosis and autophagy of lens epithelial cells was found in cases with age-related and diabetic cataract,while no expression was observed in control lens epithelial cells.Conclusion The apoptosis and autophagy of lens epithelial cells are closely related to age-related and diabetic cataract formation.

Objective To understand the source of knowledge on schistosomiasis prevention and control and the acceptable degree on different propagation patterns of population in Jingjiang City after schistosomiasis transmission?interrupted,so as to provide the evidence for the production of suitable materials for health education. Methods The permanent residents,floating population as well as the primary school students in Grade 5 and 6 in riverside regions in Jingjiang City were selected as the in?vestigation objects,the information propagation condition and the knowledge source,and the expected propagation patterns were investigated by questionnaires. Results The popularizing rates on the television,computer and mobile phone of the villag?ers were 99.81%,84.16% and 87.78%,respectively. Both the two investigated schools had the independent TV network,broad?cast and multimedia,and all the computer classroom could connected to Internet. The main sources on schistosomiasis control of permanent residents and floating population were folders and the exhibition board(62.0% and 40.5% respectively),the substan?tial material(42.0% and 33.5% respectively)and TV(40.5% and 36.0% respectively),while those of the students were video CD(100%),substantial material(99.5%)and folders and the exhibition boards(84.2%). The propagation ways with high ac?ceptable degrees among the residents and floating population were substantial material,folders and the exhibition boards and short messages by mobile phone,while those among the students were video CD,substantial material,folders and the exhibition boards. Conclusion The material on health education should be targeted to the demands of the different populations and their condition of information propagation,in addition,the Internet material should be developed and the communicational channels should be broaden.