Objective To study the method for determination of nipacide PX and triclosan in disinfectant by high performance liquid chromatography. Methods High performance liquid chromatography, based on the mobile phase (CH3CN:H2O=60:40, pH=3.0) and diode array detector(DAD, ?= 280 nm), was chosed to determine nipacide PX and triclosan in disinfectant. Results The detection limit of nipacide PX was 2.48 ng and triclosan was 2.08 ng. The rate of recovery was 95.4%-103.3%,the relative standard deviations(RSD) was 0.6%-3.4%. Conclusion The method is simple, precise and suitable for the determination of nipacide PX and triclosan in disinfectant.

BACKGROUND: As indicated by previous researches, aluminium (Al) could affect learning and memory of animals through many approaches in cluding affecting the stable status of intracellular calcium, decreasing protein kinase C(PKC) activity, and affecting the release of glutamic acid(Glu) . The formation of long-term potentiation(LTP) weakens in hip pocampal CA3 region of rats fed by forage containing Al. It could be found that Al would weaken evoked potential(EP) in hippocampal CA3 region and inhibit LTP formation, which might be related with the damaging effect of Al on L-Arg-NO approach through further application of acute Al administration, i.e., AlCl3 is directly injected into hippocampal CA3 region by microinjection.OBJECTIVE: To investigate the damaging effect of Al on learning and memory, and its correlation with cholinergic system and gamma-aminobutyric acid (GABA) system.DESIGN: A completelyrandomized controlled verifying study based on the experimental animals.SETTING: Department of psychology in a university and the medical college of an occupational technology college.MATERIALS: The study was conducted in the Laboratory of Neuro-Electrophysiology, the Faculty of Physiology, Tongji Medical College,Huazhong University of Science and Technology between September 2000 and April 2001. Totally 68 SD rats of ordinary grade in either gender with a body mass between 150 g and 250 g were obtained from the Department of Experimental Animals of Tongji Medical College, Huazhong University of Science and echnology.INTERVENTIONS: SD rats were randomly divided into 9 groups including normal NS ( NS ) control group ( n = 6): 1 μL of NS was injected twice ( 1 minute interval) into hippocampal CA3 region by microinjection; NS + AlCl3 group( n = 6): 1 μL of NS and 0.5 mol/L of AlCl3 were injected(1 minute interval) into hippocampal CA3 region by microinjection;NS + Tac group( n = 6): 1 μLof NS and 1 × 10-9 mol/L of Tacrine were injected in turn into hippocampal CA3 region by microinjection; NS + Bic group(n=6): 1 μL of NS and 1 × 10-3 mol/L of Bicuculline were injected in turn into hippocampal CA3 region by microinjection; Tac +AlCl3 group: 1 μL of 1 × 10-9 mol/L( n =8),1 × 10-10 mol/L ( n = 6) and 1 × 10-8 mol/L of Tacrine were firsdy injected into hippocampal CA3 region by microinjection, and 1 μL of 0. 5 mol/L AlCl3was injected 1 minute later; Bic + AlCl3 group: 1 μL of 1 × 10-3 mol/L( n = 9) and 1 × 10 -4 mol/L( n = 7) of Bicuculline were firstly injected into hippocampal CA3 region by microinjection, and 1 μL of 0.5 mol/L AlCl3 was injected 1 minute later. Population spike(PS) in hippocampal CA3 region was recorded after using single pulse to stimulate perforating fiber(PF). When PS became stable, medication was injected into hippocampal CA3 region to observe the impacts of Al on EP in hippocampal CA3 region and the impacts of some central transmitters on the effect of Al in in hibiting PS.MAIN OUTCOME MEASURES: PS evoked in hippocampal CA3 region;the impacts of Al on EP in CA3 region and the impacts of some central transmitters on the effect of Al in inhibiting PS. RESULTS: ① After the application of 0.5 mol/L of AlCl3 in hippocampalCA3 region by microinjection, the recorded amplitude of PS reduced to peakat 1 minute, which accounted for(33.8 ± 11. 0) % of the level beforemedication( n = 6). The inhibitive effect of AlCl3 lasted for 120 minutes. ② After the pre-application of 1 × 10-9 mol/L of Tacrine(cholinesterase in hibitor) into CA3 region by microinjection and the application of AlCl3 at oneminute later, it was found that Tacrine antagonized the inhibitive effects ofAlCl3 on PS within 1 to 30 minutes( n = 8) . Its antagonism would extend to60 minutes if 1 × 10-8 mol/L of Tacrine was administrated( n = 6) . How ever, the antagonism of 1 × 10-10 mol/L of Tacrine was weaker than that of 1×10-9 mol/L group within 3-5 minutes(n=6) ③ After thepre-application of 1 × 10-3 mol/L of Bicuculline into CA3 region by mi croinjection and the application of AlCl3 at one minute later, Bicucullinecould partially weaken the effects of AlCl3 within 1 to 20 minutes( n = 9). CONCLUSION: Al of certain concentration can inhibit the evoked PS am plitude in hippocampal CA3 region; Tacrine can antagonize Al' s effects andits antagonism might be related with dose. Hence, the inhibitive effects of Almight be related with the damage in Ach transmitter system. The applicationof Bicuculline, a GABAA inhibitor, also can weaken the PS inhibitive effectsof Al, which indicates that the inhibitive effect of Al also might be effective through GABA approach.

Objective To investigate the effects of 860MHz microwave on the formation and extinction of context conditioned fear in mice. Methods The mice were exposed to 860MHz continuous microwave ( power density were 380 μW/cm2 or 550 μW/cm2, respectively) for 30 min or 2 h, which then were divided in to 5 groups.Each group consisted of 15 animals. Footshocks were used to induce context conditioned fear by 75 voltages. The frequency and time of freezing after irradiation were investigated. Results When 24 h after foot shocking, the values of freezing time: control group was 2.31 ±4. 17 , two groups of the microwave irradiation 2 h were 3.93 ±6.99 and 2.47 ± 3.34, the Nemenyi test results (P = 0.004): control group was 32.63333, while two groups of the microwave irradiation 2 h were 52.46667 and 39.76667; and the values of freezing frequency: control group was 0.73 ± 1.16 , two groups of the microwave irradiation 2 h were 0.86 ± 1.41 and 1.07 ± 1.16, the Nemenyi test results of (P=0. 014): control group was 33. 26667, while two groups of the microwave irradiation 2 h were 50. 76667 and 40.90000. Conclusion The mice receiving relatively longer period of microwave irradiation showed more stable memory of the context conditioned fear.

Objective: To study the effects of static tensile strain on the cytoskeleton of human periodontal ligament fibroblasts (HPLFs). Methods: Tensile strain with the strain variate of 8%,12%,16% and 20% was applied in the culture of HPLFs for 1,2 and 3 d respectively with a self-devised loading apparatus. With the aid of laser scanning confocal microscope, the projected areas of each observed cell and its nuclear were calculated and the cell cytosketeton was observed. The data analysis was carried out with SPSS using Dunnett test. Results:The projected areas of the cells and nucleus incereased with the increace of loading time and tensile strain value in trial groups of 8%,12% and 16%(P

Objective This research is one of the sub-researches of"The comparative study of the standards of interventional therapies and the evaluation of the long-term and middle-term effects for common malignant tumors",which is one of the National Key Technologies R&D Program in the eleventh five-year plan. Based on the project,the authors need to establish an international standard in order to set up the national tumor interventional therapy database and registration system.Methods By using the computing programs of downloading software,self-management and automatic integration,the program was written by the JAVA words.Results The database and registration system for the national tumor interventional therapy Wag successfully set up,and it could complete both the simple and complex inquiries.The software worked well through the initial debugging.Conclusion The national tumor interventional therapy database and registration system can not only precisely teU the popularizing rate of the interventional therapy nationwide,compare the results of different methods,provide the latest news concerning the interventional therapy,subsequently promote the academic exchanges between hospitals,but also help as get the information about the distribution of the interventional physicians,the consuming quantity and variety of the interventional materials,so the medical costs can be reduced.

Objective: To explore the effect of low-level laser irradiation (LLLI) preconditioning on milieu of infarcted myocardium in experimental rats.
Methods: The myocardial infarction (MI) model was established by left anterior descending (LAD) artery ligation in female rats. 3 weeks later, the qualified MI rats were randomly divided for 3 groups: ① LLLI preconditioning group, the rats received thoracotomy for LLLI by a 635nm, 5mW diode laser with the energy density of 0.96 J/cm2 for 150 seconds, n=26. ② Control group, the rats received thoracotomy for daylight irradiation, n=27. ③ Sham operation group, the rats received thoracotomy without LAD ligation, n=24. The Expressions of myocardial vascular endothelial growth factor (VEGF), glucose-regulated protein 78 (GRP78), superoxide dismutase (SOD) and malondialdehyde (MDA) were evaluate by real time-PCR, Western blot analysis and other relevant laboratory test at 1 hour, 1 day and 1 week after treatment. The myocardial cell apoptosis was examined by TUNEL staining, and left ventricular function was detected by echocardiography.
Results: LLLI preconditioning obviously increased the myocardial VEGF, GRP78 expression and SOD activity, decreased MDA production; while it could not really improve the myocardial cell apoptosis at peri-infarcted area and left ventricular function in experimental rats.
Conclusion: LLLI preconditioning may improve the milieu of infarcted myocardium via decreasing the oxidative stress in experimental rats.

Objective: To explore the effect of omentopexy combining autologous atrial tissue patch cardiomyoplasty for treating the chronic myocardial infarction (MI) in experimental rats.
Methods:The MI model of SD rats was established by left coronary artery ligation. 3 weeks later, the qualiifed animals were randomized into 4 groups for the 2nd operation. ① Control group, the rats only received re-thoracotomy, ② Atrial appendage group, the autologous atrial tissue patch was harvested from left atrial appendage of rats and transplanted to infarcted zone, ③ Omentum group, the omentum of rats was transplanted to infarcted zone through diaphragm and ④ Combination group, the left atrial appendage tissue and omentum were transplanted to infarcted zone together. 4 weeks after the 2nd operation, the cardiac function was evaluated by echocardiography, the size and scar thickness of the infarction were examined by Masson staining, the survival of transplanted atrial tissue and angiogenesis were measured by immunohistochemistry, the protein expressions of VEGF, MMPs and TIMP-3 were examined by Western blot analysis, and the activities of MMPs were
identiifed by gelatin zymography.
Results:4 week after the 2nd operation, transplanted left atrial appendage tissue only survived in Combination group. The areas of infarction were similar among different groups, P>0.05. Compared with Control group, Combination group had the increased scar thickness (329 ± 33)μm vs (391±31)μm, improved LVEF (47.5 ± 4.5)%vs (57.9 ± 5.8)%, improved LVFS (20.7 ± 2.0)%vs (25.2 ± 3.6)%, all P<0.05. Compared with Control group, both Omentum group and Combination group demonstrated higher density of angiogenesis at infracted area (33/0.2) mm2 vs (49/0.2) mm2 and (33/0.2) mm2 vs (48/0.2) mm2, all P<0.01. Combination group had decreased protein expressions of MMPs, while the expressions of TIMP-3 were similar among different groups, the activities of MMP-2 and MMP-9 were decreased by 68%, P=0.002 and 64%, P=0.016 respectively.
Conclusion:Omentopexy could improve the angiogenesis and support the survival of transplanted autologous atrial tissue patch, therefore improve the cardiac function in experimental rats with chronic MI.

Objective: To conifrm the omentopexy decreasing the susceptibility of ventricular arrhythmia in experimental rats with chronic myocardial infarction (MI) by nerve remodeling.
Methods: The MI model of SD rats was established by left coronary artery ligation. 3 weeks later, the qualiifed animals were randomized into 3 groups for the 2nd operation. ① Sham operation group, the rats received the 2nd operation without ligation, ② Isolated MI group, the rats received the 2nd operation without omentopexy, ③ Omentopexy group, the rats received the 2nd operation with omentopexy. n=20 in each group. 4 weeks after the 2nd operation, the electrophysiological characteristics were assessed by relevant techniques, the new and sympathetic nerves in MI border zone were examined by immunohistochemistry, the protein expressions of connexin43 and nerve growth factor (NGF) were measured by western blot analysis and the cardiac endothelin-1 (ET-1) level was evaluated by ELISA.
Results: Compared with Isolated MI group, Omentopexy group showed decreased susceptibility of arrhythmia (3.5 ± 1.2) vs (0.9 ± 0.2), improved electrical transduction (1.5 ± 0.2) mV vs (3.4 ± 0.3) mV and decreased capture threshold (5.5 ± 0.3) mV vs (2.2 ± 0.2) mV, all P<0.05. Compared with Isolated MI group, immunihistochemisty indicated that Omentopexy group had decreased new and sympathetic nerves in MI border zone, as for GAP43:(1388.4 ± 244.9)μm2/mm2 vs (768.6 ± 144.1)μm2/mm2, for TH:(1552.4 ± 270.3)μm2/mm2 vs (1018.5 ± 124.7)μm2/mm2, all P<0.05. Western blot analysis showed that Omentopexy group had the lower NGF expression and higher connexin43 expression;ELISA demonstrated that Omentopexy group had the lower ET-1 expression, P<0.05.
Conclusion: Omentopexy may decrease the susceptibility of ventricular arrhythmia after MI in experimental rats, which might be related to the cardiac nerve remodeling.

Objective To investigate the role of cerebellar dentate-interpositus (D-I) nuclei during acquisition of classical eyeblink conditioning in guinea pigs. Methods A 500 ms tone conditioned stimulus (CS) was paired with a 100 ms corneal oxygen-puff unconditioned stimulus (US) in a delay paradigm. Guinea pigs were trained daily to acquire the classically conditioned eyeblink responses. Micorinjections of Muscimol into bilateral cerebellar D-I nuclei were performed during the 4-6 day of training session. A high-resolution potentiometer was used to detect the eyeblink responses. Results The conditioned response (CR) rates of Muscimol-injected group on session 4-6 were significantly lower than their rates on session 3 (P1=0.005,P2=0.004,P3=0.010); Their rates on session 7 and 8 were as the same level as those without microinjection on session 4 and 5 (P1=0.061,P2=0.669). However, there was no significant difference on their amplitude and peak latency of unconditioned responses (URs) during the entire course of training (P1=0.926,P2=0.939). Conclusion The cerebellar D-I nucleus is actively involved in the neural circuitry of motor learning, which is essential for both acquisition and performance of the classically conditioned eyeblink responses.

Objectives: To investigate the status of chlamydia pneumonia infection in blood donors from Nanjing Command of PLA. Methods: Use the technique of nested polymerase chain reaction to detect the DNA of chlamydia pneumonia in peripheral blood mononuclear cells. Results: 32 of 100 blood donors were positive (32%). Conclusions: Our study reveals that the infection rate of chlamydia pneumonia in blood donors from Nanjing Command of PLA is considerable high and the clinical values need further research.