Objective To study the protective mechanism of Buyang Huanwu decoction(补阳还五汤) on brain damage after focal cerebral ischemia/reperfusion(I/R)in aged rats from the expression changes of nuclear factor-?B(NF-?B),heat shock protein 70(HSP70)and nitric oxide synthase(NOS).Methods A focal cerebral ischemia model was established by middle cerebral artery occlusion(MCAO),and the models were duplicated.Ninety-six aged rats were randomly divided into four groups:sham-operated group,model group,nimodipine group(6 mg?kg~(-1)?d~(-1)),Buyang Huanwu decoction group(0.9g?kg~(-1)?d~(-1)).The ischemia(I)was performed for 3 hours and reperfusion(R),for 1,3,6 and 12 days.The effects of Buyang Huanwu decoction on the nervous dysfunction score,the water content of cerebral constitution and the expressions of NF-?B,HSP70 and NOS were observed at different time points of reperfusion.Results The nervous dysfunction score,the water content of cerebral constitution,the expressions of NF-?B,HSP70, endothelial nitric oxide synthase(eNOS),neuronal nitric oxide synthase(nNOS),and inducible nitric oxide synthase(iNOS)in the model group were higher than those of the sham-operated group(P

Objective To investigate the clinical efficacy of target fire red-hot needling in treating articular deformity due to rheumatoid arthritis.Methods Ninety-six rheumatoid arthritis patients with articular swelling and deformity were randomly allocated to treatment and control groups, 48 cases each. The treatment group received target scattered fire red-hot needling and the control group, conventional acupuncture. After two courses of treatment, pre-/post-treatment changes in the self-reported pain score, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and immunoglobulins (IgA, IgG and IgM) were observed in the two groups and the clinical therapeutic effects were compared between the two groups. Results The total efficacy rate was 89.6% in the treatment group and 50.0% in the control group; there was a statistically significant difference between the two groups (P<0.01). There were statistically significant pre-/post-treatment differences in ESR, CRP, IgA, IgG and IgM in the treatment groups (P<0.05). There were statistically significant pre-/post-treatment differences in ESR, CRP, and IgG in the treatment groups (P<0.05). There were statistically significant post-treatment differences in ESR, CRP, IgA, IgG and IgM in the treatment and control groups (P<0.05).Conclusion Target fire red-hot needling is an effective way to treat articular deformity due to rheumatoid arthritis.

Intensity-modulated radiation therapy is widely used in the treatment of tumor,and the dose distribution highly conforms to tumor target area in three-dimension.However,the factors such as complex beam,data error,algorithm error and machine error may cause large dose deviation in intensity-modulated radiation therapy,which may lead to unnecessary radiation accident.Therefore,standing on the patients' safety point of view,dose verification is usually performed before executing the treatment plan in order to ensure the safe implementation of the treatment plan and to avoid un-planned irradiation dose.Currently,there are many tools and methods of dose verification in clinic,including point dose verification tools like finger-shaped ionization chamber and thermoluminescence dosimeter;two-dimensional dose verification tools like Mapcheck,MatriXX and films;three-dimensional dose verification tools like ArcCHECK,Delta4 and the third-party software.These common clinical dose verification methods are reviewed in this paper.

Objective To establish the quality standard of Fengsui Pills. Methods The qualitative analysis of Fructus Amomi and Radix Glycyrrhizae in Fengsui Pills was carried out by TLC,and the content of berberine hydrochloride,an effective component of Cortex Phellodendri in Fengsui Pills was determined by HPLC. Results The chromatographic sports of Fructus Amomi and Radix Glycyrrhizae were identified without the interference of negative control. A linearity of berberine hydrochloride was obtained from 0.272?g to 1.360 ?g with a good correlation (r=0.999 9,n=5).The average recovery was 99.01 %and the RSD was 0.98%. Conclusion The qualitative and quantitative methods established in this study are simple and feasible,accurate and reproducible,and can be used as the quality control standard for Fengsui Pills.

Objective:To study the protective effect of electro-acupuncture on glutamate(GLU),glycine(GLY),aspartate(ASP)and NF-?B expression in rat with cerebral ischemia reperfusion.Methods:The middle artery occlusion(MCAO)model was established and high pressure liquid chromatography(HPLC)fluorescence assay was used to detect contents of glutamate(GLU),glycine(GLY)and aspartate(ASP)in rat brain.The effect of electro-acupuncture on the nervous dysfunction score,the water content of cerebral constitution and the expression of NF-?B were observed.The group with non-acupoint group was as control.Results:The nervous dysfunction score,the water content of cerebral constitution,NF-?B,GLUand ASP in model group were higher than those of the sham-operated group.GLY levels had no obvious changes,there was no significant diference between the model group and the non-acupoint group.The nervous dysfunction score,the water content of cerebral constitution,the expression of NF-?B,GLU and ASP in electro-acupuncture group were lighter than that of the model group,GLY levels had no obvious changes.Conclusion:Electro-acupuncture could protect brain cell from damage after focal cerebral ischemia reperfusion injury by inhibiting the cerebral expression of NF-?B and excitatory amino acid.

Objective:To study the protective effect of electroacupuncture on stem cell proliferation and differentiation in dentate gyrus (DG) in aged rats with focal cerebral ischemia reperfusion injury. Methods:We duplicated focal cerebral ischemia model with MCAO,with ischemia 3h,I/R1,3,7,14,21d points. The effect of electroacupuncture on the nervous dysfunction score,the water content of cerebral constitution and the stem cell proliferation and differentiation in dentate gyrus were observed,and the non-acupoint group was as control. Results:The nervous dysfunction score (all the time points),the water content of cerebral tissue (I/R1,3,7d),BrdU positive cells (all the time points),BrdU/NeuN positive cells (all the time points),BrdU/GFAP positive cells (I/R7,14,21d),in model group were higher than those of the sham-operated group (P

An on-site method for the determination of sulfur mustard ( SM) was developed based on pinhole shell-isolated nanoparticle-enhanced Raman spectroscopy. By using 0. 1 mol/L MgSO4 as effective agglomeration reagent, more Raman “hot spots” were induced, and thus a limit of detection for SM at 10 μg/L was achieved with a linearity of 10-1000 μg/L and an analytical enhanced factor of 1. 1×106. This method can be directly applied in the measurement of SM in environmental water samples with good sensitivity and reproducibility, and the standard addition recovery was between 88%-114%. Good differentiation of four SM related compounds, 2-chloroethyl ethylsulfide, thiodiglycol, bis-β-chloroethyl sulphoxide and bis-β-chloroethyl sulphone, was also obtained.

Objective To compare the lipoprotein(a) (Lp-a) and lipid metabolism level in rheumatoid arthritis(RA) patients and control group (healthy subjects),and evaluate the correlation between Lp-a levels and systemic inflammation in patients with RA.Methods Blood samples from 30 RA patients with positive rheumatoid factor and 30 healthy controls were collected,with same distribution of gender and age in both groups.Lipid metabolism level such as Lp-a,triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol (LDL-C),very low-density lipoprotein cholesterol (VLDL-C) and inflammation markers such as tumor necrosis factor α (TNF-α),interleukin 6 (IL-6) and C reactive protein (CRP) were detected and analyzed statistically.Results LP-a levels were higher in RA patients than that of control group(P＜0.001),HDL-C levels were lower in RA group (P＜0.05),while TC,TG,LDL-C and VLDL-C levels showed no difference (P＞0.05).The TNF-α、IL-6 and CRP levels of RA patients were higher than that of control group(P＜0.05),and the association between higher Lp-a level and TNF-α were confirmed (r=0.753,P＜0.001).Conclusions High levels of Lp-a are often observed in RA patients,and a rising Lp-a level are associated with systematic inflammation reaction.Lp-a may be a risk factor for RA progress.

Objective To explore the effects of different courses of electro-acupuncture at Baihui,Fengfu,and Shenshu of both sides on the behavior of mice exposed to radiation.Methods Fifty C57BL/6Jmice 30 days old were randomly and equally divided into control group,model group (exposure to radiation of 8 Gy),electro-acupuncture group 1 (1 week of treatment),electro-acupuncture group 2 (2 weeks of treatment),and electro-acupuncture group 3 (3 weeks of treatment).The mouse model of radiation-induced brain injury was established by exposure to radiation of 8 Gy and treated with electro-acupuncture.The open field test and Morris water maze test were used to evaluate the cognitive function and spatial learning and memory,respectively.Group t-test difference.Results In the open field test,the model group had significantly fewer vertical or horizontal movements than the control group (P=0.032,0.029,0.028),while the electro-acupuncture groups 2 and 3 had significantly more vertical or horizontal movements than the model group (P=0.001,0.017,0.000).In the Morris water maze test,compared with the control group,the model group had significantly longer latency,a significantly lower frequency of entry into the goal box,and significantly shortened residence time,indicating substantially worse performance of spatial learning and memory (P=0.023,0.001,0.001).Compared with the model group,the electro-acupuncture groups 2 and 3 had significantly shortened latency,a significantly higher frequency of entry into the goal box,and significantly longer residence time (P=0.046,0.017,0.049,0.007,0.031,0.009).Conclusions Different courses of electro-acupuncture treatment at Baihui,Fengfu,and Shenshu of both sides can substantially improve the cognitive function and spatial learning and memory in mice exposed to radiation.Particularly,2 and 3 weeks of electro-acupuncture treatment give the best outcomes.

Objective To investigate the effects of electroacupunctures at Baihui,Fengfu,and Shenshu on the cognitive function and learning memory of mice exposure with ionizing radiation.Methods C57BL/6J (30-day old) mice were randomly divided into l0 groups consisting of the control group,X-ray exposure groups (4,8 and 16 Gy),electroacupuncture-nonacupiont (E-nonA) groups (4,8 and 16 Gy) and electroacupuncture-acupiont (EA) groups (4,8 Gy and 16 Gy),with 10 mice assigned to each group.The mice were irradiated (IR) on the left cerebral hemisphere with doses of 4,8 and 16 Gy.Some mice were electroacupunctured for 3 courses of treatment.Using weight as a measurable parameter,the open-field test and Morris water maze test were used to evaluate the curative effects.Results Compared with the control group,the weights of mice in the exposure groups (8 and 16 Gy) significantly decreased (t =2.917,9.650,3.043,2.882,P ＜ 0.05) at 7 and 14 d,while the weights of the E-nonA (8 and 16 Gy) and EA groups (8 and 16 Gy) were not significantly altered.Compared to the model groups (8 and 16 Gy),the weights of EA groups (8 and 16 Gy) significantly increased (t =6.602,5.409,19.660,2.975,P ＜ 0.05).In the open-field test,the crossing times and the rearing time of exposure model groups (8 and 16 Gy) significantly decreased compared to the control group (t =2.183,2.119,2.369,2.231,P ＜ 0.05).Compared with model groups (8 and 16 Gy) and E-nonA groups (8 and 16 Gy),the crossing times and rearing time of the EA groups were significantly increased (compared with model:t =8.914,2.727,4.325,4.178,P ＜ 0.05;compared with E-nonA:t =4.030,3.014,2.150,4.771,P ＜0.05).In the Morris water maze,compared with the control,the latent period of model group (4 Gy) was significantly increased and the crossing times significantly decreased (t =4.544,3.422,P ＜ 0.05);compared to model and E-nonA groups (4 Gy),the latent period of EA group significantly increased (compared with model:t =2.877,P ＜ 0.05;compared with E-nonA:t =3.001,P ＜ 0.05).At day 2 and 3,the latent period of model groups (8 and 16 Gy) significantly increased (t =2.544,3.706,3.796,7.934,P ＜ 0.05) and the crossing times (t =2.120,2.393,P ＜ 0.05) significantly decreased,resident time in target area of model group (8 Gy) was significantly decreased (t =2.543,P ＜ 0.05) compared to the control group.Compared with model and E-nonA groups (8 and 16 Gy),the latent period of EA gourps significantly decreased (compared with model:t =5.404,7.869,4.104,15.590,P ＜ 0.05;compared with E-nonA:t =2.938,7.955,6.566,10.350,P ＜ 0.05) while the through times and resident time in target area significantly increased in the EA group (8 Gy) (compared with model:t =2.673,2.613,P ＜ 0.05;compared with E-nonA:t =3.345,2.179,P ＜ 0.05).Conclusions Irradiation with a dose higher than 8 Gy could induce mice brain injury.Electroacupuncture can alleviate the radiation damage of cognitive function as well as learning and memory functions in mice.