Objective This study was designed to investigate the effect of matrine on high glucose‐induced tumor necrosis factor‐α(TNF‐α)and transforming growth factor‐β1 (TGF‐β1 ) overexpression in rat peritoneal mesothelial cells(PMCs) ,and to ex‐plore the possible mechanism in the peritoneal fibrosis .Methods ology The rat PMCs were incubated in vitro ,and then assigned in‐to 5 groups on the basis of the added drug concentrations :2 .5% glucose group (n=15 ,A);Glucose + 2 .5% matrine group (n=15 ,B);4 .25% glucose group (n=15 ,C);Glucose + 4 .25% matrine group (n=15 ,D);Blank control group (n=15 ,control group , added the DMEM/F12 medium) .The levels of TNF‐αand TGF‐β1 in the supernatants were measured by ELISA method at the dif‐ferent time (24h ,48h ,72h) ,and observed the changes of PMCs under the microscope .Results Compared with the groups of A ,C and D ,the PMCs in the control group and B group were small ,round ,spindle ,irregular or intensive .The expression of TNF‐αand TGF‐β1 in the A group was lightly higher than that of the control group ,but the level in the C group was significantly higher than that of the control group (P<0 .05) .As compared with the A group ,The level of TNF‐α and TGF‐β1 expressed in the B group were decreased (P> 0 .05 ) ,but the lever in the D group was significantly lower than that of the C group ( P< 0 .05 ) . Conclusion Matrine might be helpful to resist the overexpression of TNF‐αand TGF‐β1 induced by high glucose ,and then protect the PMCs .

Purpose:To study if tamoxifen (TAM) can induce growth arrest and apoptosis of ER negative MA782 mouse breast cancer cell line and to explore the molecular mechanisims. Methods:MA782 cells were cultured in RPMI 1640 medium with TAM.The proliferative activity of cells was detected by MTT methods, and cells apoptosis by flowcytometric methods. The expression of cyclin D1, CDK4 and TGF ?1 proteins was detected by immunohistochemical methods, the semi quantification of protein expression was analyzed by pathological image analysis software.Results:TAM can induce growth arrest and apoptosis of cells. ICC results showed that MA782 cells were ER negative. There was no change of cell cycle regulators in cells with 2 ?mol/L TAM. After 48、72h with 6 ?mol/L or 10 ?mol/L TAM, the level of cyclin D1 proteins decreased from 132.5?0.02 to 129.67?0.03、126.18?0.03(6 ?mol/L) and 109.1?0.01、73.56?0.02(10 ?mol/L),CDK4 proteins decreased from 107.2?0.01 to 91.23?0.02、76.21?0.03(6 ?mol/L)and 83.52?0.02、72.03?0.01(10 ?mol/L), while TGF ?1 proteins increased from 59.72?0.02 to 83.2?0.04、121.75?0.03(6 ?mol/L)and 96.83?0.02、139.01?0.05(10 ?mol/L),The difference was significant( P

Purpose To summarize the clinical and echocardiographic characteristics of congenital quadricuspid aortic valves (QAV) so as to improve the understanding of QAV and the accuracy of preoperative diagnosis.Materials and Methods The clinical and echocardiographic data of 7 patients with QAV at the First Affiliated Hospital of Nanchang University were retrospectively studied,and the features such as the aortic valve (AV) leaf number,echo,shape,opening and closing movements,and its hemodynamics were observed and compared with surgical follow-ups.Results Five out of the 7 patients had chest tightness,chest pain,fluster,shortness of breath,and 2 others had no discomfort.The echocardiography presented that all the cases had moderate or severe aortic valve regurgitation.4 patients were diagnosed as QAV,2 patients were not diagnosed definitely,and the rest 1 was misdiagnosed.6 patients underwent aortic valve replacement and were all confirmed with QAV,among whom 2 patients were combined with infective endocarditis,and 1 patient was with aortic dilatation.All the surgical operations were successful and the patient's physical conditions were good after surgery.Conclusion Echocardiography plays an important role in diagnosis of aortic valve,but it is possible to be missed or misdiagnosed.Most QAV patients have good prognosis,but close follow-ups are needed when QAV is combined with other complex deformity or has induced secondary damages.

Objective To summarize the echocardiographic characteristics and clinical signiifcance of prenatal diagnosis of coronary artery ifstula (CAF). Methods Images and follow-up results of ifve fetuses with CAF diagnosed by fetal echocardiography between January, 2011 and December, 2012 in our department were reviewed. Results Echocardiographic characteristics of CAF were a dilated coronary artery in the four chamber view and the left ventricular outlfow tract view. Track the course of the dilated coronary artery can conifrm the oriifce of the ifstula. Among the ifve cases, the oriifce of the ifstula included the aortic root of left ventricular outlfow tract, right atrium side of interatrial septum, the entrance of superior vena cava to right atrium, right ventricular apex and right ventricle cone. The colour Doppler lfow imaging showed turbulence in the dilated coronary artery. The spectral Doppler with the sampling gate in coronary artery showed the characteristic bidirectional lfow pattern. One case was associated with other complex intracardiac abnormalities and one case with persistent left superior vena cava. Among the ifve cases of CAF diagnosed by fetal echocardiography, one case was missed and four cases were conifrmed by postnatal echocardiography. Conclusion Coronary artery ifstula has special fetal echocardiographic characteristics. The fetal echocardiography plays an important role in early detection, diagnosis and treatment of CAF.

Objective To investigate the expression and significance of cellular inhibitor of protein phosphatase 2A (CIP2A), bcl-2 and p63 in papillary thyroid cancer (PTC). Methods Using immunohistochemistry to detect the expression of CIP2A, bcl-2 and p63 in 30 cases of nodular goiter (NG), 30 cases of thyroid adenoma (TA) and 57 cases of PTC [including classical PTC (cPTC) 20 cases, papillary microcarcinoma (PMC) 20 cases, follicular thyroid papillary carcinoma (FPTC) 7 cases]. Results In NG group, TA group and PTC group, positive rates of CIP2A were 0, 0 and 94.74 % (54/57), respectively. The differences were statistically significant. In NG group, TA group and PTC group, positive rates of bcl-2 were 16.67 % (5/30), 13.33 % (4/30) and 85.96 % (49/57), respectively. The differences were statistically significant. In each group, positive rates of p63 were 6.67% (2/30), 3.33% (1/30) and 5.26% (3/57), respectively, no significant difference among them. In PTC, expression of CIP2A and bcl-2 were significantly higher than in NG and TA (χ2 = 105.56, P= 0.00; χ2 = 58.95, P= 0.00). Furthermore, the expression of CIP2A and bcl-2 had correlation in PTC (r=0.94, P=0.00). The expression of CIP2A, bcl-2 and p63 had no significantly difference among all the PTC subtype (χ2 values were 2.02, 2.64, 1.85; all P> 0.05). The expression of CIP2A, bcl-2 and p63 was not associated with patients'age, sex, site, lymph node metastasis (all P>0.05). Conclusions High expression of CIP2A and bcl-2 is associated with PTC, and the expression of CIP2A and bcl-2 has correlation in PTC. The expression of p63 has no correlation with PTC.

Objective To study the status of prevalence of hyperlipidaemia among rural residents aged from 30 to 59 in Changzhou and provide evidence for taking prevention measures.Methods By the means of the cluster random sampling,a questionnaire survey and medical examination were conducted among 10 018 permanent residents in rural areas of Changzhou.The fasting plasma cholesterin(TC),triglycerides(TG),high density lipoprotein-C(HDL-C) were measured.Age-standardized prevalence rates were calculated by using the direct standardization method according to 2000 China population census.Results The prevalence of hyperlipidaemia of this population was 32.3%,the age-standardized prevalence was 31.3%,and the prevalence among men was significantly higher than women(P

Purpose To detect the expression of Snai2 mRNA in gastric carcinoma and normal gastric mucosa and analyze its significance.Methods Total RNA was extracted with TRIzol from 30 cases of gastric carcinoma and mRNA was transcribed reversely into cDNA.The expression of Snai2 mRNA was examined by real-time PCR; then mRNA probes were prepared, the expression of Snai2 mRNA was detected by in situ hybridization on a tissue array,including normal gastric mucosa (20 cases) and gastric carcinoma (100 cases).Results Real-time PCR data showed they had different expression in different degrees of differentiation of the cancer: the poorer differentiation, the more expression (P<0.05). In situ hybridization results showed the positive rate in the carcinoma (54.2%) was higher than normal tissue (27.8%), with statistically significant difference (P<0.05).There were different positive rate in different degrees of differentiation: the poorer differentiation,the higher positive rate, but it had no statistical significance (P>0.05).Conclusions Snai2 could be a biomarker of malignant degree and disease progression,and used as a useful tool for evaluation of prognosis.

Objective To identify the gene locus and the mutation of DSRAD (double-stranded RNA adenosine deaminase) in a Chinese dyschromatosis symmetrica hereditaria(DSH) family. Methods After confirming the diagnosis of the DSH proband, the genomic DNA was extracted from the whole blood samples of every members of the pedigree. The DSRAD gene intervals were localized by linkage analysis and haplotype reconstruction. The mutation of DSRAD was detected by direct sequencing. Results The candidate gene was localized at the 1q region, consistent with the reported region. The direct sequencing results showed that there was a CAA→TAA transition at exon 2 of DSRAD in all affected family members, which consequently led to a nonsense mutation of Gln517Ter. Conclusion A nonsense mutation is found in the Chinese DSH family.

Objective To determine the best extraction process of ginkgo leaves with the total transfer rate of total flavonol glycosides and terpene lactones as the index.Methods The effects of ethanol concentration, solid-liquid ratio and extraction time on extraction process were investigated by orthogonal design method, and the contents of total flavonol glycosides and terpene lactones were detected by HPLC to calculate transfer rate.Results The optimum extraction conditions were as follows:85% ethanol refluxing and extracting for three times;the first time extracting with five-fold amount of solvent (V/W) for 3 hours;the last two times with three-fold solvent (V/W) for 2 hours.Conclusion This extraction process has the advantages of simplicity of operator, reason, energy conservation, high efficiency, and is suitable for industrial production.