Objective:To study the chemical constituents in the seed of Cassia leschenaultiana.Methods: The compounds were separated and purified by column chromatography , thin-layer chromatography and recrystallization .The structures were identified by the physicoche mical identification and spectral analysis .Results:Seven compounds were isolated from the seeds of Cassia leschenaultiana and identified as 1-desmethylchryso-obtusin (Ⅰ) , aurantio-obtusin (Ⅱ) , ale-emodin (Ⅲ) , obtusin (Ⅳ) , chryso-obtusin (Ⅴ) , ob-tusifoline(Ⅵ)and aurantio-obtusin(Ⅶ).Conclusion:All of the compounds are isolated from the seeds of Cassia leschenaultiana for the first time.

Objective To determine the clinical value of myocardial performance index (MPI) in the assessment of right ventricular function in the patients with atrial septal defect (ASD). Methods The study included 23 patients with ASD and 81 normal subjects. MPI, defined as the sum of isovolumic relaxation time and isovolumic contraction time derived by ejection time, was measured from tricupid inflow and right ventricular outflow Doppler velocity profiles recorded during routine echocardiography. Results ①The isovolumic relaxation and isovolumic contraction times [respectively ( 74.78 ? 13.86 )ms vs ( 60.93 ? 12.94 )ms, P

The infection of chronic refractory wound is related with bacterial biofilm .How to prevent and treat bacterial bio-film and its related damage has been a hot topic in current studies .This article provides a review on chronic wound biofilm on clinical diagnosis techniques , diagnostic criteria , bacteria identification outcomes and influencing factors .

Objective:To establish the HPLC fingerprint of Cassia leschenaultiana from different regions. Methods: The column was SinoChrom ODS-BP (250 mm × 4. 6 mm, 5 μm). The mobile phase consisted of acetonitrile-0. 1% phosphoric acid with gradient elution. The flow rate was 1. 0 ml·min-1 , the detection wavelength was 285 nm, the column temperature was 25℃, and the injection volume was 10 μl. Results: The fingerprint consisted of 13 common peaks. The range of similarity for ten batches of Cassia le-schenaultiana was 0. 839-0. 998. And the reference fingerprint of Cassia leschenaultiana was established by HPLC. Conclusion: The fingerprint method is simple and reproducible, which can provide basis for the quality control and the medicinal resources exploration.

Objective To evaluate the formation of coronary collateral circulation with intracoronary Doppler ultrasound in pigs after acute occlusion and the influence of nitroglycerin and phentolamine on it.Methods The blood flow spectrun was detected in 14 pigs using intracoronary Doppler ultrasound before and after acute occlusion of the left circumflex artery (LCX) and its response to nitroglycerin and phentolamine.The average peak velocity (APV) and the diastolic systolic velocity ratio (DSVR) were recorded. Results The APV of the LCX decreased significantly at 10 min after acute occlusion (0.97?1.05 vs 25.78?4.36 cm/s,P0.05). Conclusion Intracoronary Doppler ultrasound can be used to evaluate coronary collateral circulation. The formation collateral circulation was insufficient after acute occlusion of coronary artery in pigs and nitroglycerin or phentolamine can improve the blood flow.

Objective To investigate the cause of non-union and delayed union of femoral and tibia] fractures and assess the clinical outcome after treatment with intramedullary compression interlocking nail (ICIN). Methods From February 1998 to December 2006, 21 patients with non-union and delayed union of femoral and tibial fractures (13 patients with femoral fractures and 8 with tibial fractures) were treated by ICIN. Bone grafting was performed in 18 patients, and the other three patients only rocoived reamed compression nailing. Five patients received bone grafting combined with knee adhesion release operation. Results All patients were followed up for 11.4-36 months (mean 13.6 months). Solid bone union was observed in all patients, with mean bone union time of 8.7 months and without malunion, infection or refracture. According to the Klemm grading, the clinical outcome was graded excellent in 19 patients and good in 2. Conclusions The main causes for non-union and delayed union of femoral and tibial fractures are improper indication selection and incorrect use of implants, which may result in bieenvironment disruption. ICIN shortens the time of functional recovery of knee and ankle joint. ICIN has advantages of stable fixation, early exercise of the knee and ankle as well as early weight loading and hence is one of effective alternatives in treatment of non-union and delayed union of femoral and tibial fractures. ICIN can accelerate the healing of bone and improves the function of knee joint when combined with bone grafting, reamed compression nailing and knee adhesion release.

OBJECTIVE:To investigate the occurrence of ADR induced by over-the-counter(OTC)drug in order to guide the rational use of drugs. METHODS:45 cases of ADR induced by OTC drug were analyzed retrospectively in terms of patient's gen-eral information,allergic history,allergenic drugs,reason for drug use,route of administration,incubation period,days of hospi-talization,clinical manifestation,treatment,outcome,etc. RESULTS:Among 45 patients,there were 14 male and 31 female. The patients were mainly 20-30 year-old(31.1%). Three patients had previous history of allergy(6.7%). Most ADR were induced by oral drugs(82.2%). Reasons for drug use were mainly disease of respiratory system(23 cases,51.1%). The results of causality as-sessment were positive in 3 patients(6.7%),probable in 35(n=77.8%),and possible in 7 patients(15.6%). ADR were mainly induced by non-steroidal anti-inflammatory drugs (NSAIDs,57.8%),Chinese patent medicine (20.0%),drugs for external use (17.8%). The clinical manifestations mainly included exanthematous drug eruption (33.3%),erythama multiforme drug eruption (24.4%),urticarial drug eruption(13.3%). CONCLUSIONS:NSAIDs are the main allergenic drugs,and ADR induced by OTC traditional Chinese medicine can not be ignored. To improve medical knowledge and skills of the public and the scientific use of OTC drug are beneficial to improve the clinical treatment level.

This thesis systematically summarizes the experience of Professor YAN Xiao-ping in treating palindromic rheumatism, the following conclusions we have got: ① Palindromic rheumatism is very much like panodynia in Yellow Emperor's Internal Classic, and the treatment of this disease should according to that of panodynia; ②The primary pathological mechanism is the deficiency of spleen and kidney, and the secondary is the invading of wind evil factor accompanying with some other evil factors; ③The treatment principals including: nourishing kidney and spleen for the radical treatment, expelling the evil factor such as wind, cold, dampness and hot, assisting with pungent and warm herbs to through channels when expelling hot and clearing away dampness, moving the blood stasis all the way. Because it is shortage of literatures about this disease, the thesis has some practical significance.

Objective To study the influence of sampling,handling,shipping and storage on low-molecular-weight serum proteome profiling.Methods Serum samples instantly separated and aliquoted,some stored at-80 ℃ for up to 6 months,others stored at 4 ℃ or room temperature(25 ℃) for 2 to 72 hrs.The variations of protein profiling under these conditions on WCX magnetic beads were studied.Profiling influenced by hemolysis,multi freeze-thaw cycles,storage conditions.Results Different handling procedures and storage conditions have different effects on serum profiling.Serum stored at-80 ℃ up to 6 months,stored at 4 ℃ or 25 ℃ for 2 h or one freeze-thaw cycle,had little effects on serum proteomic analysis.If serum diluted into 9 mol/L urea buffer at room temperature,the result is stable for 24 hours.Statistics analysis shows that 35.9% peaks have significant changes(P

Objective To investigate the clinical value of color Doppler ultrasound in the early diagnosis of acute cholecystitis infection ,in order to improve the diagnosis of acute cholecystitis infection .Methods 36 cases with acute cholecystitis infection were selected as the observation group , and during the same period the healthy people were randomly selected as the control group .The color Doppler performance of ultrasound was observed .The hepatic artery blood flow velocity and the maximum peak of the cystic artery (Vmax) and resistance index(RI) were meas-ured.ROC curve was observed to evaluate the specificity of color Doppler ultrasound for the disease .Results The incidence rates of weak bile sound transmission ,gallbladder wall thickness ,gallbladder enlargement ,ultrasound Mur-phy positive in the control group were 25.0%,16.7%,22.2%,8.3%,which in the observation group were 72.2%, 47.2%,100.0%,94.4%,the differences were statistically significant (χ2 =4.571,4.571,4.589,5.689,all P<0.05).The differences in hepatic artery and the cystic artery Vmax and RI were statistically significant between the two groups(P<0.05).ROC curve comparison showed that the correct rate of gallbladder enlargement in the observa -tion group was the highest (90.7%),the specificity was 90.7%,sensitivity was 90.6%,and other indicators of ultra-sound had no significant difference (χ2 =0.893,P>0.05).Conclusion Color Doppler ultrasound can show the form of acute infectious cholecystitis ,gallbladder wall degree of bile through sound etc .,it can improve the diagnostic value of the disease.