Professor WU Lianzhong's experience of neck acupoints application is introduced. The characteristics,locations, acupuncture manipulations ,efficiency and main functions of neck acupoints including Tiandixue (Extra), Jingbixue (Extra), and cervical Jiaji (EX-B 2) are stated. According to the TCM thought of treatment based on syndrome differentiation,WU Lianzhong's special theory of neck acupoints application is explained so as to provide experience for improving acupuncture effects.
Acupuncture Points ; Acupuncture Therapy ; Aged ; Dizziness ; therapy ; Female ; Humans ; Neck ; anatomy & histology ; Qi

APOBEC3 is a class of cytidine deaminase, which is considered as a new member of the innate immune system, and APOBEC3G belongs to this family. The research about APOBEC3G is a new direction of innate immune defense mechanism against virus. APOBEC3G has the restrictive activity on many viral replications, which deaminates dC to dU in the viral genome and then induces extensive hypermutation. APOBEC3G can also interrupt viral replication at several phases such as reverse transcription, replication, nucleocapsid and so on by non-deamination mechanisms. However, virus can encode viral proteins to counteract the restriction activity of APOBEC3G. Elucidation of the antagonistic interaction between APOBEC3G and the virus will be contributed to development of new antiviral drugs in the future.

For obtaining new structural compounds with unique resistance profiles or novel mechanisms of action on HIV-1 from natural products, anti-HIV-1 drug screening models were used in vitro. Norcantharidin (NCTD), a derivative from cantharidin, was found to have inhibitory activities on HIV-1(IIIB) p24 antigen in lymphocyte lines MT-4, CEM and H9. It inhibited HIV-1 strain 018a (sensitive to zidovudine) from replicating with EC50 (50% effective concentration) of 14.9 micromol L(-1) and also inhibited HIV-1 strain 018c (resistant to zidovudine) from replicating with EC50 of 20.2 micromol L(-1) in primary lymphocytes peripheral blood mononuclear cells (PBMC). Norcantharidin showed synergistic activity with zidovudine on HIV-1(IIIB) in MT-4 cells, the combination index was less than 0.3. But, it was not active on HIV-1 integrase, reverse transcriptase or protease in vitro. As the structure of norcantharidin is unique and different from that of all clinic drugs approved, it would be possible to obtain new and effective compounds against HIV-1 with low toxicities after modification of norcantharidin.

Objective To study the effects about rhizoma drynariae poly (lactic-co-glycolic acid) (DR-PLGA) /Calcium phosphate cement (CPC) composite scaffold on treating rabbit femoral bone defect.Methods Eight Newzealand rabbits were randomly divided into experiment group(n =4) and control group (n =4).The preparation of rabbit femoral bone defect model,separately implanted DR-PLGA/CPC scaffold and PLGA/CPC scaffold.After 4,8 weeks,we took out the materials,observed with X-ray,gross anatomy,histology observation to evaluate the osteogenetic activity,the effect of accelerating the healing of bone defect.Resuits At the 4th week and 8th week after implantation,the effect of promoting fracture healing and osteogenic activity of the experiment group were greater than those in the control group.Conclusions DR-PLGA combined with CPC could induce new bone formation,promote the healing of rabbit femoral defect.

Objective To explore the value of afterdischarges monitoring for intraoperative electrical stimulation for brain mapping. Methods 34 patients received cerebral cortex electrical stimulation for brain mapping during operation of brain function area , afterdischarges were monitored simultaneously to determine the upper limit of stimulus intensity. Results 34 cases underwent electrical stimulation successfully , and received surgery without neurologic decline except 2 cases of hypokinesia. Conclusion After discharges monitoring improve the accuracy, reduce the risk of intraoperative cerebral cortex electrical stimulation.

Objective Pressure Ulcer Scale for Healing ( PUSH) was published in 1998 by National Pressure Ulcer Advisory Panel ( NPUAP) as a tool to evaluate the effects of pressure ulcer care.This study aimed to verify the reliability and validity of the Chi-nese version of PUSH in order to provide an efficient and reliable tool for evaluating the effects of pressure ulcer care. Methods Using the Brislin translation model, we translated the English version of PUSH into Chinese and finalized the Chinese version through modifica-tion by an advisory panel, pretests, and verification of its reliability and validity in the care of 126 cases of stage-Ⅱ-Ⅳpressure ulcers. We analyzed the reliability and validity of the scale based on its item scores, content validity, construct validity, Cronbach′s αcoeffi-cient, and test-retest reliability. Results The correlation coefficient value of the total and individual item scores was 0.616-0.963 (P<0.01).Each individual item score was significantly higher in the 27%high-score group (n=35) than in the 27%low-score group (n=36).The total content validity coefficient was 0.965, the correlation coefficient of construct validity between the total and individual item scores was 0.750-0.954 (P<0.01), and that between individual items was 0.666-0.826 (P<0.01), with statistically signifi-cant differences between the total and individual item scores at 7 and 21 days after treatment (P<0.01).The Cronbach′s αreliability coefficient value of the total score was 0.823 and those of individual item scores were 0.770, 0.791, and 0.868, respectively.The inter-rater reliability coefficients were all >0.85 and the test-retest reliabili-ty coefficients of individual items were 0.826, 0.885, and 0.958, re-spectively ( P<0.01) . Conclusion The Chinese version of PUSH, with its high validity and reliability, can be used to evaluate interven-tion effectiveness of Chinese patients with pressure ulcers.

Background and purpose: The protein tyrosine kinase-7 (PTK7) gene may be related to the occurrence and progression of many tumors. This study was aimed to explore the expression of PTK7 in ovarian serous tumors and its relationship with clinical stage, histological grade, metastasis and prognosis indicators linkages, and analyze the diagnostic and prognostic value of PTK7 in ovarian serous tumors. Methods:Expressions of PTK7 in 3 ovarian cell lines (HO8910, SKOV3, A2780), 14 cases of normal fallopian tube epithelium, 6 cases of benign serous ovarian tumors, 51 cases of borderline serous ovarian tumors and in 97 cases of ovarian serous carcinoma were detected by immunohistochemical EliVision two-step method. Statistical analysis of the relationship between the expression of PTK7 and the pathological indicators was performed byχ2 test, Fisher exact test and Kaplan-Meier method. Results:PTK7 was negatively expressed in HO8910 and A2780, but weakly positively expressed in SKOV3. The positive rates of PTK7 in normal fallopian tube epithelium, benign serous ovarian tumors, borderline serous ovarian tumors and serous ovarian cancer were 92.86%(13/14), 83.33%(5/6), 45.10%(23/51), and 28.87%(28/97), respectively. The expression of PTK7 had no difference between normal fallopian tube epithelium and benign serous tumors, benign serous tumors and serous borderline tumors (P=0.521, P=0.102). The PTK7 expression showed signiifcant differences in serous ovarian carcinoma compared with those in normal epithelium, benign serous tumors and borderline serous tumors (P=0.000, P=0.012, P=0.048). Expression of PTK7 in borderline serous ovarian tumors was signiifcantly with clinical stage, metastasis (lymph node and/or peritoneum metastasis) (P=0.038, P=0.038), rather than its location, age (P=0.088, P=0.896). Expression of PTK7 in ovarian serous carcinoma had a signiifcant relation with its clinical stage, WHO grade, MDACC grade (P=0.011, P=0.004, P=0.000), rather than its location, metastasis, tumor diameter and age (P=0.326, P=0.524, P=0.588, P=0.584). The survival rate of PTK7 positive group in ovarian serous carcinoma was signiifcantly higher than that in the negative control group (P=0.017). Conclusion:The expressions of PTK7 in normal ovarian epithelium, benign serous ovarian tumors, borderline serous ovarian tumors and epithelial serous carcinoma show a gradual downward trend. The expression of PTK7 in ovarian serous tumors has a positive correlation with late clinical stage, high histological grade and poor prognosis. PTK7 can be a new indicator of clinical diagnosis and prognosis in ovarian serous tumors.

Objective To observe the effectiveness of transplantation of neuron-like cells induced by GDNF from BMSCs on treatment of spinal cord injury in rats.Methods Transfected BMSCs by recombinant adenovirus containing the gene of rGDNF and GFP.36 SD rats with spinal cord injury were randomly divided into three groups.All the 36 SD rats with spinal cord injury were scored by the Basso,Beattle,Bresnahan locomotoRrating scale at different time points (1、2、3、4 weeks afteRoperation).In the 4th week afteRoperation,tissue from the injury section was prepared to observe repaiRof spinal cord injury.Results BMSCs was able to secrete GDNF continuously afteRtransfected by recombinant adenovirus containing the gene of rGDNF and GFP.AfteRoperation,BBB scores in intralesional treatment group significantly increased when compared with that of intravenous treatment group and injury control group.In the 4th week afteRoperation,HEmatoxylin-eosin staing results showed that cavitation in intravenous treatment group was largeRthan that in intralesional treatment group with the one of injury control group being the biggest.Immunohistochemical method showed that there were more NF200+cells and GFAP+cells in intralesional treatment group than in the otheRtwo groups,and injury control group had the least NF200+cells and GFAP+ cells.Conclusion AfteRinduced into nuron-like cells,BMSCs can treat spinal cord injury effectively.

Objective To provide the evidence for prevention and treatment of aspirin resistance (AR) in coronary artery disease (CAD) patients by analyzing the prevalence, related risk factors of AR and the distribution rules of TCM patterns.Methods One hundred CAD patients were eligible, who were confirmed by angiography and took aspirin at dose of 100 mg per night for at least four weeks, at the average age of(68.95±8.91) years.All the patients were divided into three groups according to the platelet aggregation assay induced by arachidonic acid (AA,1 mmol/L).The platelet aggregation rate of ≥80%, ≥50% and <80%, or ≤50% was used to define AR, aspirin semiresistance (ASR) or aspirin sensitive (AS) respectively.Meanwhile, based on the experts advice and literature retrieving, TCM pattern factors of patients were identified as qi deficiency, sputum turbid, blood stasis, qi stagnation, yang deficiency, yin deficiency and cold stagnation.Then the risk factors and distribution of TCM patterns were compared among the three groups.Results There were 12 CAD patients of AR found, whose fasting blood glucose levels, serum creatinine, quantity of lesion and severe lesion in the coronary artery were higher than those of other two groups(P<0.05),but insulin levels two hours after meal was lower(P<0.05).There were no significant difference of TCM pattern distribution among the three groups (P>0.05).And the multivariate logistic regression analysis showed that serum fasting glucose level was a significant risk factor of aspirin resistance.Conclusion AR has a relative high prevelance.Fasting blood glucose level is closely associated with AR in CAD patients.In AR group, the deficiency pattern factors included qi deficiency and yin deficiency, while the main excessive pattern factors were blood stasis and sputum turbid.Aspirin sensibility has no effects on distribution of TCM pattern factors.

Objective To observe the effectiveness of transplantation of neuron-like cells induced by GDNF from BMSCs on treatment of spinal cord injury in rats.Methods Transfected BMSCs by recombinant adenovirus containing the gene of rGDNF and GFP.36 SD rats with spinal cord injury were randomly divided into three groups.All the 36 SD rats with spinal cord injury were scored by the Basso,Beattle,Bresnahan locomotoRrating scale at different time points (1、2、3、4 weeks afteRoperation).In the 4th week afteRoperation,tissue from the injury section was prepared to observe repaiRof spinal cord injury.Results BMSCs was able to secrete GDNF continuously afteRtransfected by recombinant adenovirus containing the gene of rGDNF and GFP.AfteRoperation,BBB scores in intralesional treatment group significantly increased when compared with that of intravenous treatment group and injury control group.In the 4th week afteRoperation,HEmatoxylin-eosin staing results showed that cavitation in intravenous treatment group was largeRthan that in intralesional treatment group with the one of injury control group being the biggest.Immunohistochemical method showed that there were more NF200+cells and GFAP+cells in intralesional treatment group than in the otheRtwo groups,and injury control group had the least NF200+cells and GFAP+ cells.Conclusion AfteRinduced into nuron-like cells,BMSCs can treat spinal cord injury effectively.