Objective To investigate the effects of temperature and time on the quality of cryoprecipitate.Methods Fresh frozen plasmas were divided into the three groups,and the cryoprecipitate were prepared from fresh frozen plasma by siphonage method thawing at 2℃,4℃ or 6℃ water bat.Thawing time,FⅧ and fibfinogen (Fg) were compared among the three groups.Results The thawing time at 2℃,4℃ or 6℃ was (96.3 ± 5.9) min,(89.6 ± 4.3) min,(81.2 ± 2.7) min.The activity of F Ⅷ was (95.36 ± 4.8) IU,(91.21 ± 3.3) IU,(89.8 ±3.1)IU,respectively.The activity of FⅧ was the highest at 2℃ condition and there was significantly different compared with other two groups (t =6.94,P =0.009,t =8.96,P =0.004).The activity of FⅧ in 4℃ group and 6℃ group were comparable (t =0.669,P =0.19).The concentration of Fg at three groups were similar (258.6 ±12.6) mg,(253.3 ±8.2)mg,(255.9 ±9.7) mg (t =0.321,P =0.37;t =0.270,P =0.56;t =0.424,P =0.29).Conclusion The activity of FⅧ at 2℃ condition were the highest but the thawing time was the longest.The activity of FⅧ at 6℃ condition were lower but met quality requirements and the thawing time was very short.There was no significantly different at Fg concentration among three groups.Thus 6℃ is more suitable for blood center to prepare cryoprecipitate from fresh frozen plasmas.

Objective To review the concepts of three common scales assessing upper limbs function of children with cerebral palsy, namely Fine Motor Function Measure (FMFM), Melbourne Assessment 2 of Unilateral Upperlimb Function (MA2) and Quality of Upper Extremity Skills Test (QUEST), from the framework of Chinese version of International Classification of Functioning, Disability and Health-Child and Youth Version (ICF-CY). Methods Concepts of each item from FMFM, MA2 and QUEST were extracted independently by a rehabilitation doctor and a therapist with professional experience more than three years, and then linked with ICF-CY category accord-ing to the linking rules. The distribution of concepts of each scale linking to ICF-CY categories was investigated. Results A total of 62 con-cepts were extracted from these three scales, and 403 linkages to various ICF-CY categories. Concepts from FMFM were the most linked with ICF-CY category among them, in which nearly two-thirds (134/204) concepts linked with the categories of Activity And Participation. Concepts from QUEST principally linked with the category of joint and arm-supporting in Body Function. Concepts from MA2 linked to category of cognition function which reflected motor accuracy 7 times and motor fluency 9 times (more than 50%of the total amount of link-ages). Conclusion FMFM mainly evaluates the activity and participation associated with upper limb function in children with cerebral palsy, and may be more applicable for younger children. MA2 mainly evaluates the accuracy and fluency of upperlimb movement. QUEST mainly evaluates body function especially the joint and supporting function of upper limbs.

Objective To observe the clinical efficacy of JIN’s three needle therapy plus rehabilitation in treating mental retardation (MR).Method Seventy-two MR patients were randomized into a treatment group of 37 cases and a control group of 35 cases. The control group was intervened by conventional rehabilitation training, while the treatment group was treated with JIN’s three needle therapy in addition to the rehabilitation training. The modified Chinese version of Gesell Developmental Schedules (GDS) was adopted to observe the improvements in gross and fine motor skills, language, social function and adaptability, and the clinical efficacies were compared between the two groups. Result In the treatment group, the adaptability, gross motor skills and fine motor skills scores were significantly changed after the intervention (P<0.05). In the control group, the gross and fine motor skills scores were significantly changed after the intervention (P<0.05). After the treatment, there were significant differences in comparing the gross and fine motor skills scores between the two groups (P<0.05). In the treatment group, the total effective rates were respectively 93.1% and 62.5% in the mild-moderate and severe-extremely severe patients, versus 76.9% and 44.4% in the control group. There was a significant difference in comparing the total effective rate in the mild-moderate patients between the two groups (P<0.05).Conclusion JIN’s three needle therapy plus rehabilitation is an effective approach, and it can significantly improve the gross and fine motor skills and adaptability in mild-moderate MR patients.