To investigate the expression of TNF ?, IL 1?, IL 6 and IL 8 mRNA in the lung,and the levels of TNF ?, IL 1, IL 6 and IL 8 in serum of rats with sepsis induced acute lung injury(ALI), and to examine the effect of pentoxifylline on the production of these cytokines. The expressions of TNF ?, IL 1?, IL 6 and IL 8 mRNA were detected with dot blot.The levels of TNF ?, IL 1?, IL 6 and IL 8 were examined with ELISA. Compared to the sham operation group, TNF ?, IL 1?, IL 6 and IL 8 mRNA expression in sepsis group were increased evidently( P

Objective To investigate the efficient methods used for tracing endothelial progenitor cells(EPCs)after transplantation in injured lung tissue.Methods Thirty clean grade healthy syngeneic SD female rats were divided into three experimental groups(n =10):(1)sham group,rats treated with intravenous phosphate-buffered saline(PBS)instead of lipopolysaccharide(LPS)followed by EPCs graft;(2)PBS-treated group,rats treated with intravenous PBS after intravenous injection of LPS to produce acute lung injury;(3)EPC-treated group,rats treated with EPCs after intravenous injection of LPS to produce acute lung injury.The transplanted EPCs were got from the same genetic species of the SD male rats.Rats of each group were sacrificed 7 days after EPCs transplantation.Their whole lung tissues were harvested to detect the expression of Y-chromosome by using hybridization in situ and RT-PCR assay.Statistic package of SPSS16.0 was used for the data analysis and significant differences between means were evaluated by ANOVA analysis.Results Compared with the other two groups,positive signals of sex-determining region y were found in lung endothelium from the EPC-treated group.Conclusions Y chromosme specific probe can be one of efficient methods for tracing stem cells after transplantation.

Objective To discuss the application effects of CBI teaching method in medical English Teaching of respiration medicine. Methods Dividing 120 students of Grade 2009 in Jining Medical College into two groups including control group (60) and experimental group (60). The tradi-tional teaching method was adopted in the control group and CBI teaching method was adopted in the experimental group. After finishing the courses, medical professional English level test and standard-ized patients test were applied in the two groups. Students' English studying interests and autonomous English learning ability were also investigated by questionnalres. SPSS 17.0 software was used for sta-tistical analysis, and the data were processed by t test and 字2 test, with the level of a test equal to 0.05. Results The medical professional English level test scores, standardized patients test scores and the total average scores of autonomous English learning ability in experimental group were respectively [(81.97±4.91), (80.33±5.85), (3.88±0.38)], and they were significantly higher than those in the control group [(70.40±4.78), (70.48± 4.79), (2.98±0.26), with P values below 0.01]. Satisfaction degrees in the improvement of learning motivation, learning time, learning method, learning attitude and dealing with study difficulties in experimental group were better than those of control group (χ2=13.084, 8.000, 13.297, 11.627, 13.374, P=0.000). Conclusion CBI teaching method is better than traditional teaching method and it transfers English learning into the learning of professional knowledge and skills of respiratory medicine, stirs the interests of students, improves their autonomous English learning ability and strengthens their abilities to learn and apply English language.

Objective:To explore the role of matrix metalloproteinase 12 (MMP12) in airway macrophages and pulmonary neu-rogenic substance P ( SP ) in the pathogenesis of asthma by analyzing their relationship in different categories of asthmatic patients.Methods:Twenty patients of asthma remission phase ( remission asthma group ) , twenty ones of mild acute exacerbation asthma (mild asthma group) and twenty healthy adults (normal control group) were included,respectively.After lung function was measured,the numbers of macrophage in induced sputum were counted.The expression levels of MMP12 mRNA and protein in sputum macrophages were detected by quantitative reverse transcription polymerase chain reaction and Western blot.The concentration of sputum SP was assayed by enzyme immunometric assay.Results: ( 1 ) Compared with the subjects in normal control group, forced expiratory volume in 1 second%predicted ( FEV1 ) and forced expiratory flow rates at 50% of the forced vital capacity % predicted ( FEF50 ) were much lower and the numbers of sputum macrophages were much higher in the patients in different asthmatic groups.Compared with the patients in remission asthma group,FEV1 and FEF50 were much lower in the ones in mild asthma group.(2) MMP12 expressions in the macrophages and the concentrations of SP in sputum were significantly increased in the patients in different asthmatic groups compared with those in normal control group;Furthermore,MMP12 and SP in mild asthma group were much higher than in remission asthma.(3) In all patients from different asthmatic groups,mRNA expressions of MMP12 in the macrophages were positively correlated with the levels of sputum SP or the numbers of sputum macrophages,whereas negative correlations between mRNA expressions of MMP 12 and FEV 1 or FEF50 were observed.Conclusion: The regulatory imbalance of macrophages′MMP12 and pulmonary neurogic SP may participate in the pathogenesis of asthma and become the potential targets for asthma therapy.

Objective:To investigate thyroid function, physical growth, and psychological and behavioral development in children with congenital hypothyroidism.Methods:Thirty-two children with congenital hypothyroidism who were born in Yuyao People's Hospital from January 2014 to December 2018 were included in the observation group. Thirty healthy neonates who were born in the same period were included in the control group. Thyroid function index changes at the age of 1 year relative to at birth, physical, intellectual, and neuropsychological development and bone age at the age of 1 year were compared between the observation and control groups.Results:Thyroid-stimulating hormone level at birth was significantly higher in the observation group than in the control group [(18.23 ± 2.71) mU/L vs. (2.85 ± 0.34) mU/L, t = 30.84, P < 0.001]. Free thyroxine level at birth was significantly lower in the observation group than in the control group [(6.76 ± 1.54) pmol/L vs. (17.91 ± 2.04) pmol/L, t = 24.39, P < 0.001]. In the observation group, thyroid-stimulating hormone and free thyroxine levels at the age of 1 year were (2.68 ± 0.78) mU/L and (17.26 ± 2.11) pmol/L, respectively, which were not significantly different from those in the control group [(2.77 ± 0.63) mU/L and (17.54 ± 2.20) pmol/L, t = 0.50, 0.51, both P > 0.05]. Body weight, body length, head circumference, and bone age at the age of 1 year were (9.21 ± 1.20) kg, (79.84 ± 3.05) cm, (43.73 ± 1.42) cm, (1.01 ± 0.15) years old, respectively in the observation group, which were significantly lower than those in the control group [(10.12 ± 1.32) kg, (84.54 ± 3.41) cm, (45.85 ± 2.04) cm, (1.14 ± 0.28) years old, t = 2.84, 5.73, 4.77, 2.30, P < 0.05]. The proportion of children patients with bone age lag was significantly higher in the observation group than in the control group [21.88% (7/32) vs. 3.33% (1/30), χ2 = 4.74, P < 0.05]. There was a significant difference in intellectual development at the age of 1 year between the two groups ( χ2 = 7.05, P < 0.05). Gross movement, fine movement, adaptability, language ability, and social ability in the observation group were scored (90.43 ± 6.96) points, (92.03 ± 6.03) points, (88.45 ± 4.85) points, (84.04 ± 5.71) points, and (85.05 ± 6.17) points, respectively, which were significantly lower than those in the control group [(99.47 ± 5.40) points, (104.12 ± 5.71) points, (98.47 ± 5.22) points, (94.16 ± 4.98) points, and (104.34 ± 5.70) points ( t = 5.69, 8.09, 7.84, 7.42, 12.76, all P < 0.001]. Conclusion:Neonate patients with congenital hypothyroidism have obvious physical growth and psychological and behavioral development disorders. Early screening and treatment of neonatal congenital hypothyroidism should be strengthened to improve the prognosis.

Objective To investigate the etiology of acute recurrent pancreatitis (ACP) and de-termine how to further enhance its level of treatment.Methods The clinical data of 33 patients with ACP treated in Ruijin Hospital from 2003 to 2007 were retrospectively analyzed.Results Of the 33 patients with an average age of 55 (22-86), 18 (55%) were male and 15 (45%) female.ACP occurred once in 26 patients, twice in 4 and 3 times in 3.The disease appeared whithin 1 year in 29 patients, 1-2 years in 2, 2-3 years in 1 and 3 years in 1 after being dischared from hospital.For its etiology, it was of biliary origin in 29 patients, hyperlipidemia in 1, pancreatic tumor in 1 and unknow reasons in 2.Twenty-four patients were treated with operation or endoscopy.Two patients died and the mortali-ty was 9.1%.Conclusion ACP is mainly due to biliary origin in China.Operative intervention at an appropriate opportunity can effectively reduce the recurrence of biliary-origin pancreatitis.

Objective To establish a gene sequencing method for ABO blood group,to analyze the mu-tation sites at the DNA level in order to accurately identify ABO blood group.Methods Twenty blood sam-ples were selected,in which 18 samples were ABO normal blood group and 2 samples were the ABO subtype. Exons 6 and 7 of ABO blood group gene were amplified by sequence-specific primer PCR (PCR-SSP),and then the gene sequence was directly sequenced and analyzed by PCR,and the ABO blood group was identified by comparing with the ABO reference sequence.Results The gene sequencing results of 20 blood samples were consistent with the serological results.In 2 subtype samples,the genotype in 1 sample was BA.02/O.01 and its phenotype was B(A) subtype.C>G mutation occurred at position 700 of the 7th exon,which resulted in proline changing to alanine at position 234 during amino acid translation.The genotype of the other sample was AW.37/B.01 and the phenotype was AxB subtype.The position 940 of the 7th exon mutates from ade-nine to guanine,resulting in the mutation of lysine changing to glutamic acid at position 314 during protein translation.Conclusion A method of ABO blood group gene sequencing suitable for laboratory is estab-lished,which could accurately identify ABO blood group.

Objective To analyze the dynamic changes in serum adiponectin(APN)levels after acute myocardial infarction(AMI)and its relationship with heart failure(HF)and major adverse cardiovascular events(MACE).Methods A total of 124 AMI patients who underwent percutaneous coronary intervention(PCI)in our hospital from January 2021 to December 2023 were enrolled as AMI group.Additionally,30 patients with non-AMI coronary artery disease and 30 healthy individu-als were included in non-AMI coronary artery disease group and healthy group,respectively.General information such as age,gender,smoking,alcohol consumption,hypertension,diabetes,and blood lipids were recorded for all subjects.Serum APN levels were measured using the enzyme-linked im-munosorbent assay(ELISA)method;plasma brain natriuretic peptide(BNP)levels were determined by immunofluorescence;and left ventricular ejection fraction(LVEF)was assessed using echocardio-graphy.Changes in serum APN levels and its relationship with BNP,LVEF,and MACE were recor-ded during a one-year follow-up period.Results AMI patients had lower serum APN levels than those in the non-AMI coronary artery disease group and the healthy group,while levels of total choles-terol(TC),low-density lipoprotein cholesterol(LDL-C);Lipoprotein-associated phospholipase A2(Lp-PLA2),and the proportions of smokers and diabetics were higher(P＜0.05).A one-year follow-up of AMI patients revealed an upward trend in serum APN and BNP levels and a downward trend in LVEF levels at one week,one month,three months,six months,and one year postoperatively(P＜0.05).APN and BNP levels peaked one month after PCI,while LVEF reached its lowest level at the same time point.However,there were no statistically significant changes in these indicators in the subsequent 11 months(P＞0.05).The incidence of MACE in AMI patients at one,three,six,and twelve months postoperatively was 2.8％,3.2％,4.6％,and 8.8％,respectively.Both preoper-ative and one-month postoperative serum APN levels had high predictive value for the occurrence of HF and MACE within one year in AMI patients,and the area under the curve for predicting HF and MACE one month postoperatively was larger than that preoperatively(P＜0.05).Conclusion Ser-um APN levels in AMI patients show an upward trend after PCI,peaking one month postoperatively.At this time point,serum APN levels have the highest predictive value for the occurrence of HF and MACE.

Objective To analyze the dynamic changes in serum adiponectin(APN)levels after acute myocardial infarction(AMI)and its relationship with heart failure(HF)and major adverse cardiovascular events(MACE).Methods A total of 124 AMI patients who underwent percutaneous coronary intervention(PCI)in our hospital from January 2021 to December 2023 were enrolled as AMI group.Additionally,30 patients with non-AMI coronary artery disease and 30 healthy individu-als were included in non-AMI coronary artery disease group and healthy group,respectively.General information such as age,gender,smoking,alcohol consumption,hypertension,diabetes,and blood lipids were recorded for all subjects.Serum APN levels were measured using the enzyme-linked im-munosorbent assay(ELISA)method;plasma brain natriuretic peptide(BNP)levels were determined by immunofluorescence;and left ventricular ejection fraction(LVEF)was assessed using echocardio-graphy.Changes in serum APN levels and its relationship with BNP,LVEF,and MACE were recor-ded during a one-year follow-up period.Results AMI patients had lower serum APN levels than those in the non-AMI coronary artery disease group and the healthy group,while levels of total choles-terol(TC),low-density lipoprotein cholesterol(LDL-C);Lipoprotein-associated phospholipase A2(Lp-PLA2),and the proportions of smokers and diabetics were higher(P＜0.05).A one-year follow-up of AMI patients revealed an upward trend in serum APN and BNP levels and a downward trend in LVEF levels at one week,one month,three months,six months,and one year postoperatively(P＜0.05).APN and BNP levels peaked one month after PCI,while LVEF reached its lowest level at the same time point.However,there were no statistically significant changes in these indicators in the subsequent 11 months(P＞0.05).The incidence of MACE in AMI patients at one,three,six,and twelve months postoperatively was 2.8％,3.2％,4.6％,and 8.8％,respectively.Both preoper-ative and one-month postoperative serum APN levels had high predictive value for the occurrence of HF and MACE within one year in AMI patients,and the area under the curve for predicting HF and MACE one month postoperatively was larger than that preoperatively(P＜0.05).Conclusion Ser-um APN levels in AMI patients show an upward trend after PCI,peaking one month postoperatively.At this time point,serum APN levels have the highest predictive value for the occurrence of HF and MACE.