ObjectiveTo investigate the clinical efficacy of nape eight needles plus cephalic ashi point acupuncture in treating cervicogenic headache.MethodA randomized controlled trial was carried out. Eighty-nine patients with cervicogenic headache were randomly allocated to two groups, the treatment group of 45 cases and the control group of 44 cases. Nape eight points [bilateral Fengchi(GB20), Fengfu(GV16), Dazhui(GV14) and “nape Sihuaxue”] and cephalic ashi points were selected inthe treatment group and Huatuo jiaji(Ex-B2) points at the affected cervical vertebrae and points Tianzhu(BL10), Fengchi, Yuzhen(BL9), Lieque(LU7), Taichong(LR3)and Zulinqi(GB41), in the control group. Every group was treated three times a week for two weeks. A follow-up was performed at one month after the completion of treatment. Headache was scored using the VAS, and continuous headache time, headache frequency and range of motion (ROM) of cervical spine were scored before treatment, at the end ofone week’s treatment, at the completion of treatment and at one month after the completion of treatment. The therapeutic effect at the completion of treatment and the follow-up was evaluated according to criteria for assessing the therapeutic effect.ResultAt the completion of treatment and the follow-up, the headache score and the range of motion of cervical spine score were lower in the treatment group than in the control group (P<0.05). In the treatment group, the total efficacy rate was 91.1% at the completion of treatment and 86.7% at the follow-up; the short-term and the long-term therapeutic effects were superior to those in the control group (P<0.05). After one week of treatment, the total efficacy rate was 57.8%, which was higher than 11.4% in thecontrol group (P<0.05).ConclusionTreatment with nape eight needles plus cephalic ashi point needle retention has a marked improving effect on headache symptoms and range of motion of cervical spine in cervicogenic headache. Its short-term and long-termtherapeutic effects are superior to those of conventional acupuncture. It has a higher efficacy rate and effect-producing speed.

Objective To investigate and analyze the constitution and health condition of senior high school graduate students,and to find methods to improve and promote teenagers' health.Methods Seventy-four thousand six hundred and twenty-four senior high school students graduated from the vear 2003 to 2007 were taken the health examination according to common university and college recruitment examination standard by special medical personnel.The whole data were statistically analyzed.Results The data showed that there were 30 cases of acute viral hepatitis,41 cases of infiltrative pulmonary tuberculosis,5 cases of congenital heart diseases,the rate of hepatitis B virus(HBV)infection was 9.0%(6959/74 624),nearsightedness 82.0%(61 082/74 624);and that the rate of purely qualification,unqualified quantity and specialty limit rate(contain Single disease accumulated) for registering was 14.0%,72 cases and 93.0%(69 593/74 624) respectively.Conclusion The constitution of senior high school graduate students was unsatisfactory.

OBJECTIVETo investigate the relationship between alterations of p16(INK4a) and p14(ARF) genes and gastric carcinogenesis.

METHODSThe tumors and neighboring gastric tissues from 48 patients with gastric cancer were studied. The homozygous deletion, mutation, methylation of the CpG islands, and mRNA expression of p16(INK4a) and p14(ARF) genes were assessed by PCR, PCR-SSCP, PCR based methylation assay, and RT-PCR.

RESULTS(1) The homozygous deletion rate of p16(INK4a) and p14(ARF) was 35.4% (17/48), and no homozygous deletion was examined in any gastric tissue neighboring the tumor. (2) There was no point mutation of p16(INK4a) and p14(ARF) in 31 gastric cancers without homozygous deletion or in the matched gastric tissues adjacent to the tumor. (3) Methylation of the CpG islands of p16(INK4a) and p14(ARF) was detected in 47.9% (23/48) of gastric cancers, while methylation was observed only in 2 of 48 gastric tissues neighboring the cancer with a significant difference (P < 0.01). (4) The loss rate of p16(INK4a) mRNA was 47.9% (23/48) in gastric cancer, and the patients of the combined methylation of exons 1alpha and 2 had a higher loss rate (100%, 6/6) of p16(INK4a) mRNA than those of the methylation of the other exons (11.8%, 2/17, P < 0.01); the loss rate of p14(ARF) mRNA was 45.8% (22/48) in gastric cancer, and patients with the combined methylation of exons 1beta and 2 had a higher loss rate (100%, 3/3) of p14(ARF) mRNA than those of the methylation of the other exons (15%, 3/20, P < 0.05). (5) The combined loss of p16(INK4a) and p14(ARF) mRNAs was examined in 1 (5.6%) of 18 patients of well and moderately-differentiated carcinomas, and 11 (36.7%) of 30 patients of poorly and not-differentiated carcinomas with a significant difference (P < 0.05).

CONCLUSIONp16(INK4a) and p14(ARF) genes are frequently inactivated by homozygous deletion and methylation of the 5'CpG islands in gastric cancer, which may play an important role in the carcinogenesis of gastric cancer.

Adolescent ; Adult ; Aged ; Cyclin-Dependent Kinase Inhibitor p16 ; genetics ; DNA Methylation ; Gene Deletion ; Genes, p16 ; Humans ; Middle Aged ; RNA, Messenger ; analysis ; Stomach Neoplasms ; genetics ; Tumor Suppressor Protein p14ARF ; genetics

Objective To discuss the clinical values of two-dimensional and three-dimensional diagnoses of nontraumatic esophageal hiatal hernia (HH) in middle-aged and elderly people. Methods Thirty-two cases underwent two-dimensional and three-dimensional ultrasonic examination with fasting and water iflling methods in half-sitting, standing, supine and supine right anterior oblique positions. The ifndings were compared with X-ray barium meal examination, endoscopy and operation. Results There were 27 cases sliding HH, 3 cases paraesophageal HH and 2 cases short HH. Traditional two-dimensional manifestations were as follows:widening of hiatal diaphragmatic muscles (1.6±0.4 cm) and echoes of gastric mucosa in superior hernial sac and inside hernial sac. Real-time three-dimensional surface image formations were as follows: along the long axis of diaphragmatic hiatus, superior phrenic sac was showed as mushroom-shaped or pond-shaped and the neck of hernial sac was showed as irregular tunnel-shaped or ditch-shaped;looking upward from the fundus of stomach, superior phrenic hiatus was showed as caved-shaped or louver-shaped;looking downward from mediastinal surface of lung, uperior phrenic hiatus was showed as piercer-shaped. Conclusions Two-dimensional abdominal ultrasound can diagnose nontraumatic HH in middle-aged and elderly people conveniently and accurately. Real-time three-dimensional abdominal ultrasound can display the range, size, and shape clearly. Compared with the former, the latter can provide more information for diagnosis and help ultrsound doctors and clinical doctors to understand and master the anatomical and morphopathological features of this disease.