Objective A retrospective study was carried out for the analysis of correlation of traditional Chinese medical syndrome patterns of asthma patients at period of onset with five-element motions and six climatic factors. Methods All of the subjects were the inpatients of the First Affiliated Hospital of Guangzhou University of Chinese Medicine admitted from January 20, 2011 to January 19, 2014, matching the year of Xinmao, Renchen, Guisi correspondingly in ancient Chinese calendar. The patients were confirmed as asthma at period of onset and were differentiated into various traditional Chinese medical syndrome patterns. The time of onset was classified into various Yunqi periods according to the theory of five-element motions and six climatic factors, and then the correlation of syndrome patterns with the Yunqi periods was analyzed. Results A total of 308 inpatient case files were collected. In the year of Xinmao (2011) , 31 cases were differentiated as cold pattern asthma, 48 as heat pattern asthma, 6 as turbidity pattern asthma, 5 as wind pattern asthma. In the year of Renchen (2012) , 37 cases were differentiated as cold pattern asthma, 58 as heat pattern asthma, 13 as turbidity pattern asthma, 7 as wind pattern asthma. In the year of Guisi (2013) , 32 cases were differentiated as cold pattern asthma, 47 as heat pattern asthma, 14 as turbidity pattern asthma, 10 as wind pattern asthma. The results of statistical analysis showed that the differences of the distribution of syndrome patterns were insignificant among the year of Xinmao, Renchen, Guisi (P>0.05) . During the 3 years, heat pattern asthma had the highest incidence, cold pattern asthma came next, and turbidity pattern asthma and wind pattern asthma came last. Distribution of syndrome patterns of asthma in the year of Xinmao was consistent with the law of five-element motions and six climatic factors of the year of Xinmao. On the aspects of the theory of six Jianqi ( climatic periods) , the distribution of patterns of asthma in the climatic periods of Chuzhiqi, Erzhiqi, Sizhiqi was consistent with the law of climatic periods, while that in the climatic periods of Sanzhiqi, Wuzhiqi, Liuzhiqi was not consistent with the law of climatic periods. In the year of Renchen, syndrome pattern distribution was not consistent with the law of five-element motions and six climatic factors of the year of Renchen. In the climatic periods of Chuzhiqi, Erzhiqi, Sanzhiqi, the distribution of patterns of asthma was not consistent with the law of climatic periods, while that in the climatic periods of Sizhiqi, Wuzhiqi, Liuzhiqi was consistent with the law of climatic periods. In Guisi year, the distribution of syndrome pattern was not consistent with the law of five-element motions and six climatic factors of Guisi year either. On the aspects of the theory of six Jianqi, except for Erzhiqi, the distribution of patterns of asthma in the other five climatic periods was consistent with the law of climatic periods. Conclusion The theory of five-element motions and six climatic factors has some significance in predicting the pathogenic characteristics of asthma and in guiding the prevention and diag nosis of asthma, but convinced conclusion needs more proof from large-sample clinical trial.

Objective To investigate the application of prenatal ultrasonograpy in diagnosis of fetal heart disease during the medium-term and terminal pregnancy,and to avoid misdiagnosis.Methods The prenatal ultrasonographic screening was conducted in 7020 pregnant women with medium-term and terminal pregnancy.All confirmed and suspicious cases of fetal heart disease were observed by echocardiography once 4 weeks until postnatal sixth month to 1 year while normal cases was traced at the intervals of 8 weeks.All the cases were confirmed by autopsy or surgery while part of them were confirmed by postnatal angiocardiography before surgery.The reasons of misdiagnosis were analyzed.Results In fifty-three cases of fetal cardiovascular abnormality,43 cases were found,2 cases were misdiagnosis while 8 cases were missed diagnosis.2 cases of misdiagnosis included 1 case of pulmonary valve stenosis and 1 case of tetralogy of Fallot.8 cases of miss diagnosis included 2 cases of inferior vena cava in ectopic drainage and 6 cases of ventricular septal defect (defect diameter ＜ 5 mm).The accuracy rate of diagnosis of fetal heart disease was 81.1 ％ by echocardiography.Conclusions Using high frequency probe,image zoom,enhancing the detection of vein-atrium connection and the structure of right ventricular outflow with pulmonary artery are important factors to avoid missed diagnosis and incomplete diagnosis.

Objective To investigate the application of transthoracic echocardiography (TTE) and 320-row CT in diagnosis of complex congenital heart disease(CCHD).Methods A retrospective analysis was performed in 68 patients with CCHD,which contained the echocardiographic apprarance,320-row CT and surgical outcoming.All of malformations were divided into three groups:intracardiac group,heart-large vascular connecting group and extracardiac group.Results One hundred and eighty-four malformations were comfirmed by surgery in the 68 cases.The misdiagnosis and missed diagnosis rate of CT and TTE:for the intracardiac anomalies were 12％ (11/93),1％ (1/93) respectively (P ＜ 0.05),for the heart-large vascular connecting malformation were 4％ (2/49),6％ (3/49) respectively (P ＞ 0.05),and for the extracardiac anomalies were 2％(1/42),19％(8/42) respectively (P ＜0.05),for the total anomalies were 8％(14/184),7％ (12/184) respectively (P ＞0.05).While it was 1％ (2/184) in diagnosis of total anomalies by both CT and TTE.Conclusions Both 320-row CT and TTE have their own advantages and shortcomings in the diagnosis of CCHD.Joint application of two methods could reduce the rate of misdiagnosis and missed diagnosis of CCHD.

Objective To explore the current status of humanistic care among medical students in clinical practice in obstetrics and gynecology department and its related influencing factors and to propose the corresponding countermeasures.Methods Scale of humanistic care quality was used in the survey for clinical practice students in obstetrics and gynecology department.ResultsThe total score rate of human care quality of medical students before clinical teaching were (84.12 ± 9.24)with a scoring rate of 72％.Significant differences were observed in the medical students with different medical professional(P ＜0.05).The total score of humanistic care quality of medical students after clinical teaching were (96.41 ± 1 1.53 ),significant higher than that of before training ( P ＜ 0.05 ).ConclusionHumanistic care quality in medical students needs to be strengthen.Clinical training in obstetrics and gynecology department combined with humanities education has important practical significance to improve the quality of medical students.

OBJECTIVE:To study the therapeutic efficacy of Yixinkangtai capsules(YXKT)on climacteric syndrome in males.METHODS:360cases of male climacteric syndrome randomly divided into2groups:trial group(220cases)was treated with YXKT for3wks and control group(140cases)received placebo.RESULTS:In the trial group,the rate of symptomatic improvement was74.3%.Before and after3-week treatment,blood testosterone levels were(131.51?19.12)mg/L and(253.78?21.45)mg/L;SOD levels were(1068?121.4)IU/(g?Hb)and(1178.1?132.6)IU/(g?Hb),MDA levels were(7.6?0.8)?mol/L and(5.8?0.6)?mol/L respectively,and depression score was improved as well.There were significant dif?ferences in all above-mentioned parameters between2groups.CONCLUSION:YXKT is effective and safe for treating cli?macteric syndrome in males.

AIM: To evaluate the expression of iNOS mRNA in different invasion ability colon carcinoma cell strains. METHODS: MTT was used to detect the growth and reproduction of colon cancer cell strain CW-2 and LS174T. RT-PCR and Northern blot were used to detect expression of iNOS mRNA in colon cancer. RESULTS: MTT growth curve displayed that colon cancer cell strain LS174T grew and reproduced faster than cell strain CW-2. RT-PCR showed that iNOS mRNA expressed strongly in CW-2 cell strain, while iNOS mRNA expressed weakly in LS174T cell strain. Northern blot detected that iNOS mRNA expressed obviously in CW-2 cell strain, but cell strain LS174T have no obvious iNOS mRNA expression. All-trans retinoic acid (ATRA) had no obvious effect on iNOS mRNA expression in CW-2 cell strain of colon cancer. CONCLUSIONS: ATRA has no obvious effect on iNOS mRNA expression. iNOS has a dual effect on tumor growth. In low-metastatic colon carcinoma CW-2, iNOS may exert a anti-tumor influence by cytotoxicity or inducing cell apoptosis. In high-metastatic colon cancer LS174T, iNOS produced low concentration of NO, which may be an important signal-transduction molecule for increasing blood supply and angiogenesis, which improve the growth, invasion and metastasis of tumor.

Objective: The inhibition on the proliferation of colorectal carcinoma cell strain with different proliferative potential by ATRA was investigated in present study, which would benefit for the therapy of ATRA on colorectal carcinoma. Methods: The proliferation of LS174T and CW-2 colorectal carcinoma cell strain inhibited by ATRA was determined using cell observation, FACS and MTT methods. Results: The growth speed of LS174T cell strain was faster than that of CW-2. ATRA played a significant role on inhibiting the proliferation of LS174T and CW-2 cell strain and inducting the cell differetiation in vitro. Conclusions: ATRA could inhibit the growth of LS174T and CW-2 cell strain. ATRA could inhibit the proliferation of colorectal carcinoma cell and induce cell differentiation to some extent, which was correlated with the concentration of ATRA.

Objective To determine the diagnostic significance of detecting the specific epithelial keratin CK 20 mRNA in peripheral venous blood from patients with bladder carcinomas. Methods Reverse transcription coupled with two step polymerase chain reaction (nested RT PCR) was used to detect CK 20 mRNA expression in the peripheral blood from patients with bladder carcinomas. Results Detection of CK 20 mRNA expression was positive in 37 of 91 patients with bladder carcinoma (41%).Among 20 patients with distant metastasis,17 were positive (85%).CK 20 mRNA was not detectable in the blood samples from 25 normal individuals.The frequency of positive CK 20 mRNA expression was signficantly higher in those with distant metastasis. Conclusions The presence of CK 20 mRNA expression in peripheral blood may be used as an early indicator of hematogenous metastasis of bladder carcinoma cells.

Objective To explore clinical and radiographic outcomes of unstable distal clavicle fractures (Neer ⅡB) fixed with lateral clavicle anatomic locking compression plate (LCP).Methods Between January 2009 and October 2010,eleven consecutive patients with unstable fractures of the distal clavicle (Neer ⅡB) were treated using lateral clavicle anatomic LCP.There were 9 men and 2 women,with the mean age of 37.2 years (range,23-43 years).The right shoulder was involved in 6 patients and the left in 5 patients.The interval between injuries to operation was 24-72 h (mean,48 h).After fracture reduction,the plate was place on superior of the distal clavicle.According to the distal fragment length,3 to 6 locking screws were carefully inserted,3 locking screws were used to fix proximal fractures.Coracoclavicular ligament was not repaired.Functional recovery of the shoulder joint was assessed using the American Shoulder and Elbow Surgeons (ASES) rating scale score.Plain radiographs of clavicles were used to assess bony union.Results All the patients were followed up for 9 to 12 months (mean,10.3 months).Solid bony union was eventually achieved in all patients.The mean ASES scores were 89.1 (range,84-91) on the injured side versus 96.2 (range,94-100) on the contralateral side.No implant-related fracture,fixation failure and rotator cuff injury occurred.Conclusion Lateral clavicle anatomic LCP fixation in the treatment of distal clavicular fractures is a reliable and simple technique.

Objective To investigate gene expression profile in peripheral leucocytes of patients with severe preeclampsia (SPE) during 16-20 gestational weeks to see if there are different expression between normal pregnancy and SPE, and to provide the evidence for predicting the pathogenesis of preeclampsia in the future. Methods Eight hundred primipara who accepted pregnancy examination at the First Affiliated of Hospital SUN YAT-SEN University from August 2008 to December 2008 were selected into this study. The gestational age of all objects were confirmed as 16-20 weeks by ultrasonography. And they were followed up until delivered. Six patients developed severe preeclampsia (SPE group); and 40 pregnant women without any complications were chosen as the control. Human genome complementary DNA (cDNA) single-fluorescent chip were used to detect the different gene expression in peripheral leucocytes between normal pregnancy and SPE at 16-20 gestation weeks. Results There were different expressions in 983 genes between SPE group and control group, among which 719 genes were up-regulated and 264 genes were down-regulated in the SPE group. Up-regulating genes mainly involved in immunity, coagulation and fibrinolysis, signal transduction, cell adhesion, transcription and protein synthesis; and the expression of platelet and T cell activation antigen 1 (PTA1/CD226), bactericidal/permeability increasing protein (BPI), interleukin-8 (IL-8), protein kinase C (PKC), lymphocyte antigen 75 (LY-75), mucoprotein and EGFR pathway substrate 8 (EPS8) were significantly increased in SPE patients. Down-regulating genes mainly involved in apoptosis, calcium metabolism, lipid metabolism and cell transformation; and the expression of adrenomedullin (ADM), killer cell immunoglobulin-like receptors (KIR), vitamin D receptor (VDR), adipose differentiation-related protein (ADRP), parathyroid hormone (PTH) and mitogen-activated protein kinase kinase 4 (MKK4) were significantly decreased in SPE patients. Conclusions The gene expressions of peripheral leucocytes in pre-eclampsia patients were different from those of normal pregnant women during 16-20 gestational weeks. Gene CD226, BPI, IL-8, PKC, ADM, KIR and VDR might participate in the pathogenesis of SPE which should be further investigated.