Objective To study the effect of ? interferon in the treatment of renal cell carcinoma after radical nephrectomy. Methods From Jan 1988 to Jan 1998 ,46 cases of renal cell carcinoma were randomized into two groups: the interferon group treated by radical nephrectomy and ? interferon and the control group with radical nephrectomy alone. Results The T lymphocyte subsets CD 3,CD 4,CD 8,the ratio of CD 4/CD 8 were significantly different berfore and after treatment in the interferon group( P

Objective To study the volume of residual urine as related to the surgical indication for BPH. Methods Urodynamic study was carried out in 103 BPH patients who were assigned to group A (no residual urine),group B (residual urine0.05) but significantly different between group C and A or between B and A (P60 ml should be strictly followed.Early surgical intervention should be undertaken if the bladder ontlet obstruction or bladder function deteriorated by BPH causing residual uine.Other causes such as neurogenic should be ruled out before surgery.

OBJECTIVE:To study the effects of exogenous abscisic acid(ABA)on salt-alkali tolerance of Scrophularia ning-poensis seedlings,and to provide theoretic evidence for cultivating S. ningpoensis in salt-alkali soil. METHODS:50 S. ningpoensis seedlings were divided into control group,salt-alkali group(75 mmol/L),salt-alkali(75 mmol/L)+ABA low-concentration,medi-um-concentration and high-concentration groups(10,50,100 μmol/L),with 10 plants in each group. 20 days after transplanting, the plants were sprayed with drugs every 4 days. Growth indexes of the plants(length of stem,fresh weight,dry weight,survival rate),physiological indexes [the contents of chlorophyll,soluble sugar (SS),soluble protein (SP) and free proline (Pro)],the contents of MDA and H2O2,the activities of antioxidase [the activities of SOD,POD,CAT,GR] and the contents of Na+ and K+in stem and root were determined in each group 2 weeks later. RESULTS:Compared with control group,stem height and survival rate of S. ningpoensis seedlings,SP,the contents of Na+ and K+ in the stem,the contents of Na+,CAT and GR in the roots were all decreased significantly in salt-alkali group;while chlorophyll,Pro,MDA and H2O2 contents,the content of K+ in the roots and the activities of SOD and POD were all increased significantly(P<0.05 or P<0.01). Compared with salt-alkali group,stem height of S. ningpoensis seedlings,fresh weight and chlorophyll content were increased significantly in salt-alkali+ABA medium-concentra-tion group,while MDA and the content of Na+ in the stem were decreased significantly;dry weight,the contents of SS and SP, the activities of SOD and CAT were increased significantly in salt-alkali+ABA low-concentration and medium-concentration groups, while the SS content was increased in salt-alkali+ABA high-concentration group;stem height and dry weight were decreased signifi-cantly in salt-alkali+ABA high-concentration group;Pro content of salt-alkali+ABA low-concentration and high-concentration groups were decreased significantly;H2O2,Na+ content in the roots were decreased significantly in salt-alkali+ABA low-concentra-tion,medium-concentration and high-concentration groups;while GR activity,K+ content in the stem and roots were increased sig-nificantly;POD activity were decreased significantly in salt-alkali+ABA medium-concentration and high-concentration groups. CON-CLUSIONS:The addition of a certain concentration of exogenous ABA can effectively increase salt-alkali tolerance of S. ningpoen-sis seedlings and strengthen the ability of the plant adapting to salt-alkali environment.

OBJECTIVE:To study the resistance physiological responses of seedling of Scrophularia ningpoensis to the stress caused by saline and alkali at different concentrations. METHODS:The seedling of S. ningpoensis was placed in the illumination in-cubator,in which the temperature was maintained at(20±2)℃,light-dark cycle at 13 h/11 h and illumination intensity at 2 000 lx. The alkali (NaHCO3) of 50,100,150,200 and 250 mmol/L and the saline (NaCl) of 50,100,150,200 and 250 mmol/L were poured once every 4 days for 16 consecutive days. The growth indexes(plant height,fresh weight,dry weight),chlorophyll (Chl) content,antioxidative indexes [superoxide dismutase (SOD),catalase (CAT),peroxidase (POD),hydrogen peroxide (H2O2),malonaldehyde (MDA),membrane penetration (RMP)],and osmotic regulation physiological indexes [soluble sugar (SS),soluble protein(SP)and proline(Pro)] of the seedling were determined. There set a blank control group(distilled water). RESULTS:For saline concentration higher than 50 mmol/L and alkali concentration higher than 150 mmol/L,the seedling height, fresh weight and Chl content were lower;for saline concentration higher than 100 mmol/L and alkali concentration higher than 150 mmol/L,the dry weight of the seedling was lower. For saline concentration of 50 mmol/L and alkali concentration of 150 mmol/L, the activities of SOD and POD were the strongest;for saline concentration of 150 mmol/L and alkali concentration of 50 mmol/L, the activity of CAT was the strongest. The contents of H2O2 and MDA increased and the content of SP decreased with the increase in saline-alkali concentration. The level of RMP increased therewith,that is to say,membrane stability decreased. For saline concen-tration higher than 100 mmol/L and alkali concentration higher than 150 mmol/L,the accumulation of SS terminated;for saline and alkali concentrations higher than 100 mmol/L,the accumulation of Pro terminated,however,the content of Pro was markedly high-er in the above-said alkali concentration than in that saline concentration. CONCLUSIONS:Saline-alkali stress causes the tissues of S. ningpoensis to produce active oxygen and its cytoplasmic membrane system to be damaged. Chl,enzymatic antioxidants such as SOD,CAT and POD,and osmoprotectants such as Pro and SS can buffer saline-alkali stress to some degree. The maximum concen-trations of NaCl and NaHCO3 that seedling of S. ningpoensis can tolerate are 50 mmol/L and 150 mmol/L respectively.

AIM: To study the cell apoptosis and the change of microstructure and ultrastructure in epididymis with experimental varicocele (EVC) in rats. METHODS: Experimental varicocele model was induced by partial ligation of the left renal vein in adolescent Sprague-Dawley Wistar rats. Apoptotic cells were detected by in situ terminal deoxynucleotityl transferase-mediated dTUP nick end labeling (TUNEL) technique. The corpus epididymis of the rats was prepared for light and electron microscopic observation. The microstructure and ultrastructure of the epididymis were studied. RESULTS: There was certain proportion of apoptosis cells in epididymis cells in control rats. The incidence of apoptosis increased remarkably in experimental group than that in control group (P

Objective:To explore the current situation of demoralization syndrome among young and middle-aged female breast cancer patients with radiochemotherapy after radical resection and to analyze its influencing factors.Methods:From January 2019 to September 2019, this study selected 204 young and middle-aged female breast cancer patients of Breast Surgery at a ClassⅢ Grade A hospital in Jilin Province as subjects by convenience sampling. All patients were investigated with the General Information Scale, Demoralization Scale-Mandarin Version (DS-MV) , Medical Coping Modes Questionnaire (MCMQ) and the Social Support Rate Scale (SSRS) . A total of 204 questionnaires were sent out and 192 valid questionnaires were collected.Results:Among 192 young and middle-aged female breast cancer patients, the score of DS-MV was (33.72±14.23) ; the total score of MCMQ was (42.49±8.83) , scores of confronce, avoidance and resignation dimension were (19.12±4.10) , (14.28±3.26) and (9.39±3.48) respectively; the total score of SSRS was (35.39±6.23) . Multiple linear regression analysis showed that the influencing factors of demoralization of breast cancer patients included the age, education level, time after surgery, confronce coping, resignation coping and social support.Conclusions:Demoralization of young and middle-aged female breast cancer patients with radiochemotherapy after radical resection has a high level. Medical staff should provide targeted intervention for patients with young age, low level of education, short time after surgery, facing disease with the resignation coping style and low level of social support so as to improve their physical and mental health.

Objective To assess the efficacy and safety of ultrasound-guided percutaneous puncturing gastric wall implantation of 125 I seeds in advanced pancreatic carcinoma . Methods Sixty-five cases ( 65 tumors ) of advanced pancreatic carcinoma implanted 125 I seeds by ultrasound-guided percutaneous puncturing gastric wall were retrospectively analyzed . The change of length diameter of tumor and pain relief were evaluated . Results The total 65 cases were performed successfully according to the preoperative plans . In the following 3 months after the operation ,the results showed that complete remission (CR) was achieved in 19 cases ,partial remission( PR) was 33 ,stable disease( SD) was 10 and the progressive disease ( PD) was 3 . The overall response rate was 80％ . About the relief of pain ,complete relief patients were 36 , partial response patients were 11 and the invalid patients were 5 . The pain relief rate was 90 .38％ . There were no bleeding ,pancreatic fistula ,biliary fistula ,gastric perforation which need to do the surgery therapy . Two cases of pancreatic pseudocyst occurred when reviewed in the third month after the operation . Conclusions It is effective and safe to adopt ultrasound-guided percutaneous puncturing gastric wall implantation of 125 I seeds in advanced pancreatic carcinoma .

Purperse:To study the effect of α interferon in the treatment of renal cell carcinoma after radical nephrectomy.Methods:From Jan 1988 to Jan 1998,46 cases of renal cell carcinoma were randomized into two groups: the study group were treated by radical nephrectomy and α-interferon while the control group with radical nephrectomy alone.Results:The T lymphocyte subsets CD3,CD4,CD8,the ratio of CD4/CD8 are significantly different berfore and after treatment in the study group (P＜0.05); the 3 year(90.5%) and 5 year (89.5%) survival rate in patients of the study group are higher than that of the control group (P＜0.05).Conclusions:α-interferon therapy after radical nephrectomy can restore celluar immunity so as to enhance the treatment efficacy.

Objective:To construct the index system for nurses' job competency in children's dental general anesthesia treatment, so as to provide a reference for formulating job admission assessment standards and nurses training strategies.Methods:Research was carried out from July to November 2021. Taking the "job competency model for specialist nurses-iceberg model" as the theoretical framework, the first draft of the indicators was initially prepared by the method of literature review and behavioral event interview. Through the Delphi method, 26 stomatology experts at home and abroad were inquired by letters. Combined with the analytic hierarchy process, the evaluation indicators and weights of the index system for nurses' job competency in children's dental general anesthesia treatment were determined.Results:In the first round, 26 experts completed the letter inquiries, and in the second round, 24 experts completed the letter inquiries, the effective recovery rates of the questionnaires were 100.00% and 92.31%, respectively, the expert authority coefficients were 0.844 and 0.856, and the Kendall harmony coefficients were 0.242 and 0.289, respectively. Finally, the index system for nurses' job competency in children's dental general anesthesia treatment was formed, including 4 first-level indicators, 11 second-level indicators, and 58 third-level indicators.Conclusions:The index system for nurses' job competency in children's dental general anesthesia treatment is scientific, reliable, and in line with clinical practice. It can provide quantitative standards for the comprehensive training of children's dental general anesthesia treatment with nurses and job admission assessment, so as to promote the effectiveness of quality of nursing and safety management.

Di-(2-ethylhexyl) phthalate (DEHP) is an ubiquitous environmental contaminant because of its extensive use in plastics and its persistence. As an environmental endocrine disruptor, it is suspected to interfere with neurodevelopment in people. However, evidence of the effects of maternal DEHP exposure on cerebellar development in offspring is scarce. The objective of this study was to investigate maternal exposure to DEHP and its effect on apoptosis of cerebellar granule cells (CGCs) and related mechanisms. Pregnant Wistar rats were administrated DEHP (0, 30, 300 and 750 mg/kg/d) by gavage from gestational day (GD) 0 to postnatal day (PN) 21. Primary CGCs were also exposed to mono-(2-ethylhexyl) phthalate (MEHP), the main metabolite of DEHP, for 24 h with concentrations of 0, 25, 100 and 250 µM. The CGCs of male offspring from 300 and 750 mg/kg/d DEHP exposure groups showed significantly increased apoptosis. In addition, the PI3K/AKT signaling pathway was inhibited in the male offspring of the 300 and 750 mg/kg/d DEHP exposure groups. However, effects on female pups were not obvious. Apoptosis was also elevated and the PI3K/AKT signaling pathway was inhibited after primary CGCs were exposed to MEHP. Furthermore, apoptosis was reduced after treatment with the PI3K/AKT signaling pathway activator, insulin-like growth factor (IGF) 1, and increased after treatment with LY294002, an inhibitor of the PI3K/AKT signaling pathway. These results suggested that maternal DEHP exposure induced apoptosis in the CGCs of male pups via the PI3K/AKT signaling pathway, and the apoptosis could be rescued by IGF1 and aggravated by LY294002.
Apoptosis* ; Diethylhexyl Phthalate ; Female ; Humans ; Male ; Maternal Exposure ; Plastics ; Rats, Wistar