BACKGROUND: Incidence of cardiac pathological changes is high under acute cerebral damage, which affects its prognosis. The massive increase of plasma monoamine transmitters might be one of the key reasons of the dependent effects of the heart after cerebral apoplexy.OBJECTIVE: To investigate the effects of monoamine transmitters on the cardiac injury in cerebral hemorrhage in rats for the exploration of the mechanism for the generation and development of cerebral-cardiac syndrome(CCS).DESIGN: A prospective, randomized and controlled study using experimental animals as subjects.MATERIALS: Study was conducted in the Laboratory of Neurology of Daping Hospital. Seventy healthy Wistar rats in either gender with a body mass between 250 g and 300 g were obtained from the animal center of Daping Hospital affiliated to Third Military Medical University of Chinese PLA.INTERVENTIONS: 1 μL of normal saline containing collagenase(l μ/μL) and heparin(7 μ/μL) was injected into the right caudate nucleus of the rats to establish cerebral hemorrhage animal model, while 1 μL of normal saline was injected into the rats of control group.MAIN OUTCOME MEASURES: Plasma noradrenaline (NE) content was analyzed by high-potent electrochemical method, changes of serous creatine kinase-MB (CK-MB) were analyzed by enzyme reaction rate method.RESULTS: Both plasma NE and serous CK-MB significantly elevated at 6 hours since the formation of hematoma in the brain of the rat, which were (2. 782 ± 0. 244) μg/L and(3. 504 ± 0. 742) μkat/L respectively. Plasma NE and serous CK-MB most significantly increased at 24 hours, the peak of hematoma after cerebral hemorrhage, which were(3. 517 ± 0. 200) μg/L and(5. 474 ± 1. 540) μkat/L respectively. NE and CK-MB gradually decreased afterwards, and plasma NE returned to preoperative level at 72 hours [ (1. 663 ±0. 094) μg/L] while serous CK-MB still higher than preoperative level [ (2. 713 ± 0. 526) μkat/L].CONCLUSION: Peripheral NE content elevation might participate the generation and development process of CCS.

Objective To observe the effect of Tongmai Capsules(TC) on high-sensitivity C-reactive protein(hs-CRP) in acute coronary syndrome(ACS) patients.Methods Fifty-four ACS patients were randomized into the treatment group(N=26) and the control group(N=28).The two groups were given routine treatment for coronary heart disease and the complications.Additionally,the control group was given the placebo,and the treatment group was given TC,the treatment lasting one month.The change of the symptom scores was observed before and after treatment,and the hs-CRP level at the admission day,on the 7th day of treatment and after treatment for one month was also detected.Results(1) The score of symptoms of ACS was improved to various degrees in both groups(P0.05) while was decreased in the treatment group(P

Objective: To establish a quantitative determination method for β-eudesmol in Cortex Magnoliae Officinalis by GC. Methods:β-Phenethanol was used as the internal standard substance;the column was a Zebron ZB-WAX capillary column ( 60 m × 320 μm,0. 5μm) with the column temperature of 200℃;the detector was FID and the vaporizer temperature was 250℃; the carrier gas was nitrogen with the flow rate of 1. 3 ml · min-1 and the split ratio was 4 ∶1. Results: The linear range of β-eudesmol was 0. 015 1-0. 271 2 mg·ml-1(r=0. 999 8);the average recovery was 99. 28%(RSD =1. 17%, n=6). Conclusion:The method is simple and accurate with good reproducibility, which can be used for the quality control of medicinal materials and decoction pieces of Cortex Magnoliae Officinalis.

BACKGROUND: In recent years, more and more scaffold materials have been used to repair soft tissue defects, and the clinical repair effect of soft tissue defects is strongly associated with the source and performance of materials. OBJECTIVE: To summarize the research progress of preparation and application of different biological scaffolds in the field of oral and maxillofacial soft tissue defect repair. METHODS: PubMed and Medline were searched for articles published from 1966 to 2019 with the English key words of “materials, scaffold, biological scaffold, soft tissue, coloboma, tissue engineering, review”. Chinese journal full-text database and Chinese science citation database were retrieved for articles published from 2003 to 2019 with key words of “material, scaffold, biological scaffold, soft tissue, defect, tissue engineering, review”. RESULTS AND CONCLUSION: Natural bio-scaffold materials are directly derived from organisms with pretty biocompatibility. Natural bio-scaffold materials can release cytoactive factors, promote cell adhesion, proliferation and differentiation, and can be combined with synthetic polymer materials with controllable properties to form composite scaffolds, which is an ideal scaffold material for repairing soft tissue defects of oral and maxillofacial regions. Nanomaterials have higher biological activity than other scaffold materials and can promote the adhesion and proliferation of seed cells, providing ideal three-dimensional space for cell growth, but their applications are currently mainly reflected in bone tissue repair, and the applications in soft tissue repair are very few. At present, there are many researches on natural biological scaffold materials in oral and maxillofacial soft tissue repair, mainly including small intestinal submucosa, acellular dermal matrix and acellular vascular scaffolds. The combination of natural biological scaffold materials and synthetic polymer materials will be a major research trend in materials for repairing oral and maxillofacial soft tissue defects.

Disseminated Intravascular Coagulation ; Hematologic Diseases ; Humans ; Immunotherapy, Adoptive

AIM: To observe the clinical efficacy of 8-0 polypropylene scleral-sutured fixed intraocular lens(IOL)suspension implantation with the double knots technique in aphakic eyes.METHODS: Retrospective case series study. The data of 30 aphakic cases(31 eyes, 22 males)that underwent IOL suspension in our hospital from January 2021 to November 2022 were collected. The suspension of IOL(AcrySof IQ or Tecnis ZCB00)was performed by 8-0 polypropylene scleral-sutured with the double knots technique. The visual acuity, intraocular pressure(IOP), IOL position and complications with at least 6 mo of follow-up were observed.RESULTS: The mean preoperative uncorrected visual acuity(UCVA, LogMAR)and best-corrected visual acuity(BCVA, LogMAR)were 2.53±0.78 and 0.35±0.26, respectively, which were 0.58±0.26 and 0.36±0.27 at 6 mo postoperatively, respectively. And the differences in UCVA were statistically significant(t=15.408, P<0.01), whereas the difference in BCVA was not(t=-1.677, P=0.104). There were no intraoperative complications, with IOL position all centered, but 3 eyes had IOL tilt, 2 eyes had intraocular hypertension, 5 eyes had corneal edema, and 1 eye had suture exposure postoperatively. There were no complications such as hyphema, vitreous hemorrhage, macular edema, corneal endothelial decompensation, hypotony, choroidal detachment, retinal detachment, fulminant superior choroidal hemorrhage, endophthalmitis, or others.CONCLUSION: The 8-0 polypropylene scleral-sutured fixed intraocular lens suspension implantation with the double knots technique can improve the postoperative visual acuity of aphakic patients, and fewer complications, which is an option for the treatment of aphakia, dislocation of the lens and ligament abnormalities.

AIM:To establish a nomogram model to predict the effect of serum ferritin on diabetic retinopathy and evaluate the model.METHODS:A total of 21 variables, including ferritin, were screened by univariate and multivariate regression analysis to determine the risk factors of diabetic retinopathy. A nomogram prediction model was established for evaluation and calibration.RESULTS:Ferritin, duration of diabetes, hemoglobin, urine microalbumin, regularity of medication and body mass index were included in the nomogram model. The consistency index of the prediction model with serum ferritin was 0.813(95%CI: 0.748-0.879). The calibration curves of internal and external verification showed good performance, and the probability of the threshold suggested by the decision curve was in the range 10% to 90%. The model had a high net profit value.CONCLUSIONS:Serum ferritin is an important risk factor for diabetic retinopathy. A new nomogram model, which includes body mass index, duration of diabetes, ferritin, hemoglobin, urine microalbumin and regularity of medication, has a high predictive accuracy and could provide early prediction for clinicians.

Interleukin-27 (IL-27), a heterodimeric cytokine, plays a protective role in diabetes. Ghrelin, a gastric hormone, provides a hunger signal to the central nervous system to stimulate food intake. The relationship between IL-27 and ghrelin is still unexplored. Here we investigated that signal transducer and activator of transcription 3 (STAT3)-mechanistic target of rapamycin (mTOR) signaling mediates the suppression of ghrelin induced by IL-27. Co-localization of interleukin 27 receptor subunit alpha (WSX-1) and ghrelin was observed in mouse and human gastric mucosa. Intracerebroventricular injection of IL-27 markedly suppressed ghrelin synthesis and secretion while stimulating STAT3-mTOR signaling in both C57BL/6J mice and high-fat diet-induced-obese mice. IL-27 inhibited the production of ghrelin in mHypoE-N42 cells. Inhibition of mTOR activity induced by siRNA or rapamycin blocked the suppression of ghrelin production induced by IL-27 in mHypoE-N42 cells. siRNA also abolished the inhibitory effect of IL-27 on ghrelin. IL-27 increased the interaction between STAT3 and mTOR in mHypoE-N42 cells. In conclusion, IL-27 suppresses ghrelin production through the STAT3-mTOR dependent mechanism.

Arrhythmogenic cardiomyopathy (ACM), a fatal heart disease characterized by fibroadipocytic replacement of cardiac myocytes, accounts for 20% of sudden cardiac death and lacks effective treatment. It is often caused by mutations in desmosome proteins, with Desmoglein-2 (DSG2) mutations as a common etiology. However, the mechanism underlying the accumulation of fibrofatty in ACM remains unknown, which impedes the development of curative treatment. Here we investigated the fat accumulation and the underlying mechanism in a mouse model of ACM induced by cardiac-specific knockout of Dsg2 (CS-Dsg2 ^-/-). Heart failure and cardiac lipid accumulation were observed in CS-Dsg2 ^-/- mice. We demonstrated that these phenotypes were caused by decline of fatty acid (FA) β-oxidation resulted from impaired mammalian target of rapamycin (mTOR) signaling. Rapamycin worsened while overexpression of mTOR and 4EBP1 rescued the FA β-oxidation pathway in CS-Dsg2 ^-/- mice. Reactivation of PPARα by fenofibrate or AAV9-Pparα significantly alleviated the lipid accumulation and restored cardiac function. Our results suggest that impaired mTOR-4EBP1-PPARα-dependent FA β-oxidation contributes to myocardial lipid accumulation in ACM and PPARα may be a potential target for curative treatment of ACM.