1. Anti-Hepatoma activity of wogonin on HepG2 cell in vitro
Chinese Pharmaceutical Journal 2013;48(12):968-971
OBJECTIVE: To study the growth inhibitory and apoptosis-inducing effect of wogonin on hepatocarcinoma cell line HepG2. METHODS: HepG2 cell line was treated with various concentrations of wogonin. Proliferation was determined by MTT test and morphology observation. Flow cytometry was applied to detect the wogonin-induced apoptosis and to analyse the cell cycle. Western blot was used to detect HDAC1 and HDAC2 expression. RESULTS: Wogonin had a growth inhibitory and apoptosis-inducing effect on HepG2 cell. The cell showed G2/M arrest by cell cycle analysis. A characteristic apoptosis change including nuclear disintergration and chromatin agglomerate was displayed. Western blot results show increase of cleave PARP, but not change in HDAC1, HDAC2 expression. CONCLUSION: Wogonin has a growth inhibitory and apoptosis-inducing effect on hepatocarcinoma cell line HepG2.
2.The value of transcutaneous oxygen saturation in early diagnosis of newborns with critical congenital heart disease
Zhao YU ; Jian LUO ; Hongtao ZHONG
Chinese Journal of Neonatology 2016;11(5):361-363
Objective To study the value and significance of transcutaneous oxygen saturation monitoring from different parts in early diagnosis of neonatal critical congenital heart disease. Methods The transcutaneous oxygen saturation in right upper extremities and right lower extremities of neonates born in our hospital from July 2010 to July 2015 were detected at 2 hours to 24 hours after birth. If the transcutaneous oxygen saturation ≤ 0. 95 or difference between upper and lower extremities≥0. 03, color doppler ultrasound echocardiography were done. Results There were 24 385 cases of newborns in our study. 1 680 newborns with abnormal transcutaneous oxygen saturation ( ≤0. 95 or difference between upper and lower extremities ≥0. 03) were examined with color doppler ultrasound echocardiography. 204 cases of congenital heart disease (12. 1% ) contain 9 cases of critical congenital heart disease ( 0. 5% ) were detected. The critical congenital heart disease included complete transposition of great artery ( n = 3), tetralogy of Fallot ( n = 4), artery malformation ( n = 1), and pulmonary atresia with right ventricular dysplasia ( n = 1 ) . Conclusions Transcutaneous oxygen saturation monitoring from different parts has a high value for early detection of critical congenital heart disease in the newborn. It is recommended for clinical use in grass-roots hospitals.
4.Porcine surfactant in treatment of LPS-induced early-stage acute lung injury in rats
Yiqun WANG ; Yu SUN ; Rui YANG ; Jigen ZHONG ; Jian LU
Chinese Journal of Pathophysiology 2000;0(08):-
AIM: To evaluate the therapeutic efficacy of intratracheal instillation of porcine pulmonary surfactant(PPS) in rats with lipopolysaccharides(LPS)-induced early-stage ALI in this study.METHODS: SD rats weighing 200 g-300 g were randomly divided into 4 groups: LPS(1.5 mg?kg-1)+saline,LPS+PPS 100 mg?kg-1,LPS+PPS 150 mg?kg-1,LPS+PPS 200 mg?kg-1.The PaO2 and PaCO2,as well as survival rate of rats were examined for 6 h after the start of PPS-instillation.Then,rats were killed and lungs were immediately removed for lung index(LI) and histological analysis.The bronchoalveolar lavage fluid(BALF) was collected for measurement of total protein(TP) contents,TNF-? level and white blood cell(WBC) numbers.RESULTS: Significantly increased PaO2,reduced mortality rate,decreased total protein and TNF-? contents in BAL,as well as lung index and meliorated histological appearance were observed in three PPS-treated groups compared with group given saline after LPS(P
5.Release of arachidonic acid metabolites from blood by cultivation of human amniotic fluid with oneself blood
Jian YANG ; Yanghong YU ; Fengqing ZHOU ; Mei ZHONG
Chinese Journal of Pathophysiology 2000;0(12):-
AIM: To investigate the effect of human amniotic fluid on the release of thromboxane A 2 (TXA 2), prostaglandin I 2 (PGI 2) and Leukotriene C 4(LTC 4) from blood cells. METHODS: 1 mL human amniotic fluid and 10 mL oneself blood collected from 38-41 weeks with cesarean section were cultured at 37℃ for 30 min, and then centrifuged. The supernatants were taken and stored at -70℃. TXB 2 and 6-Keto-PGF 1? of the superntants were determined by radioimmunoassay and LTC 4 by enzyme immunoassay. RESULTS: It was found that the levels of TXB 2 and LTC 4 in blood were elevated from (63.5?52.0) ng/L and (40.1?39.2) ng/L to (189.1?102.0) ng/L and (293.5?206.1) ng/L respectively (P0.05).CONCLUSION: Amniotic fluid might stimulate the release of TXA 2 and LTC 4 from blood, it might affect the balance of TXA 2 and PGI 2 in blood, which might play an important role in the pathogenesis of amniotic fluid embolism.
6.Influencing factors of infantile spasms relapse after remission
Zhaoshi YI ; Jian ZHA ; Huaping WU ; Xiongying YU ; Jianmin ZHONG
Chinese Journal of Applied Clinical Pediatrics 2016;31(12):899-902
Objective To analyze the potential risk factors of infantile spasms (IS) relapse through following up the respondents with IS after different treatment protocols.Methods Sixty-nine cases were collected in the Department of Pediatric Neurology,Jiangxi Children's Hospital from May 2011 to September 2013,who had complete cessation of spasms for at least 28 days or more after the different treatment protocols.The follow-up was performed on these patients until spasms seizure relapse or at least 1 year for those without recurrence.According to the literature review,8 possible risk factors of IS recurrence (gender,age of onset,course of diseases,etiology,high irregular types of electroencephalogram,development quotient,onset time,treatment protocols) were selected,and then Logistic multiple regression was used to analyze the relationship of various potential risk factors with the relapse of spasms.Results (1) The recurrence rate at 6 months and 12 months were 40.6% (28/69 cases)and 43.5 % (30/69 cases),respectively.(2) Among the various potential factors,the age at onset and the time to response were closely related to the IS recurrence.Namely,the non-classic onset(early-onset and late-onset) of IS were more likely to relapse than the classic onset[66.7% (14/21 cases) vs 33.3% (16/48 cases),x2 =6.605,P =0.010];the responders beyond 1 week were more likely to relapse than those within 1 week[63.6% (14/22 cases) vs 34.0% (16/47 cases),x2 =5.341,P =0.021].There were significant differences between the 2 groups (all P < 0.05).(3) Logistic multiple regression analysis demonstrated that age at onset (Wald =3.603) was most closely related to the relapse of spasms.Conclusions (1) The relapse rate of IS in children was high,and the majority of them relapsed within 6 months.So a long-term,rational and effective clinical management solution should be explored.(2) The age at onset and the time to response are very important risk factors of the IS recurrence,and the former was more significant.So,early diagnosis and early treatment are more likely to improve the efficacy of IS,and reduce the risks of recurrence and improve the prognosis.
7.Great attention should be paid to the adverse drug reactions associated with the use of molecular targeted anticancer drugs.
Zheng-tang CHEN ; Yu-zhong DUAN ; Jian-cheng XU
Chinese Journal of Oncology 2009;31(12):881-884
Antineoplastic Agents
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adverse effects
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therapeutic use
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Diarrhea
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chemically induced
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Drug Delivery Systems
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methods
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Exanthema
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chemically induced
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Humans
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Leukopenia
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chemically induced
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Lung Diseases, Interstitial
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chemically induced
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Myocardial Infarction
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chemically induced
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Neoplasms
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drug therapy
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Tumor Lysis Syndrome
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etiology
8.Effects of cripto siRNA on liver metastasis of colorectal cancer cell in nude mice
Ximing ZHONG ; Yu FAN ; Yongjing ZHOU ; Jian CHEN ; Gengjin LIN
Fudan University Journal of Medical Sciences 2010;37(2):202-206
Objective To study the effects of cripto on migration, invasion, and liver metastasis of colorectal cancer cell. Methods After human colorectal cancer cell line SW480 was transfected by cripto small interfering RNA (siRNA), the mRNA and protein level were determined by Real-time RT-PCR and Western blot, respectively. The migration and invasion ability were evaluated by wound-healing assay and boyden chamber model, respectively. Thirty nude mice model of liver metastasis from colorectal cancer was established by splenectomy. Results The siRNA could down-regulate the level of mRNA and protein of cripto in a dose- and time-dependent manner. Suppression of cripto expression could inhibit migration and invasion ability of human colorectal cancer cell in vitro. The metastastic rate and tumor nodules were lower in transfection with cripto siRNA than in two control groups in vivo. Conclusions Cripto gene might play an important role in regulation of liver metastasis from colorectal carcinoma cell, and suppression of cripto gene by siRNA can inhibit liver metastasis of colorectal cancer.
9.Dimethylformamide induced impairment of function of liver and kidney in exposed workers and its effect on lipid metabolism.
Ju-xiang XIANG ; Jian-zhong YU ; Xin-hui ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(7):404-406
Adolescent
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Adult
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Dimethylformamide
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adverse effects
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Female
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Humans
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Kidney
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drug effects
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physiopathology
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Lipid Metabolism
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drug effects
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Liver
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drug effects
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physiopathology
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Male
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Middle Aged
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Occupational Exposure
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adverse effects
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Young Adult