2.Determination of γ-aminobutyric acid in human plasma by LC-MS/MS and its preliminary application to a human pharmacokinetic study.
Yao CHEN ; Xiao-Jian DAI ; Jiang-Bo DU ; Kan ZHONG ; Xiao-Yan CHEN ; Da-Fang ZHONG
Acta Pharmaceutica Sinica 2014;49(11):1593-1599
A rapid, sensitive and convenient LC-MS/MS method was developed for the determination of γ-aminobutyric acid (GABA) in human plasma. d2-γ-Aminobutyric acid (d2-GABA) was synthesized as internal standard (IS). After extraction from human plasma by protein precipitation with acetonitrile, all analytes were separated on a Luna HILIC column (100 mm x 3.0 mm, 3 μm) using an isocratic mobile phase of water: acetonitrile: formic acid (20 : 80 : 0.12) with a flow rate of 0.5 mL x min(-1). Acquisition of mass spectrometric data was performed in multiple reaction monitoring mode (MRM) in positive electrospray ionization using the transitions of m/z 104 --> 69 for GABA and m/z 106 --> 71 for d2-GABA. The method was linear in the concentration range of 5.00 to 1 000 ng x mL(-1). The intra- and inter-day precisions were within 9.9%, and accuracy ranged from 99.1% to 104%, within the acceptable limit across all concentrations. The method was successfully applied to a pharmacokinetic study of GABA tablets in healthy Chinese volunteers.
Chromatography, Liquid
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Humans
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Tandem Mass Spectrometry
;
gamma-Aminobutyric Acid
;
blood
4.Significance of C-Reactive Protein Monitoring to Guide the Course of Treatment with Antibiotic in Neonatal Bactenal Infection
xiao-jian, ZHOU ; xian-wei, CHEN ; zhong-quan, LU
Journal of Applied Clinical Pediatrics 2004;0(08):-
0.05). There was only 2/109 cases (5.8%) need a second course of antibiotics because of likely infection and 102/109 cases (93.5%)need not any moor antibiotics. The mean period of antibiotic treatment in group Ⅰ, group Ⅱa and group Ⅱb were (1.2?0.5) days,(4.8?0.8) days and (9.3?1.8) days,respectively.There were significant differences(all P
5.Alagille syndrome in 5 Chinese children.
Jian-She WANG ; Xiao-Hong WANG ; Zhong-Lin WANG
Chinese Journal of Pediatrics 2007;45(4):308-309
Alagille Syndrome
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pathology
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Child, Preschool
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China
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Female
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Humans
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Infant
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Male
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