Adult ; Angiolymphoid Hyperplasia with Eosinophilia ; Female ; Humans ; Male ; Middle Aged

Objective: To compare the effects of needle pricking therapy plus rotating-pulling manipulation and rotating-pulling manipulation on blood velocity,angiokinesis,and blood viscosity in patients with vertebral artery type of cervical spondylosis(CSA),and discuss its mechanisms.Methods: 216 patients were randomly divided into 2 groups according to numeric representation,108cases in the treatment group were treated by needle pricking therapy plus rotating pulling manipulation,and 108 cases in the control group were treated by rotating pulling manipulation.The peak systolic blood velocity(Vpeak),end diastolic blood velocity(Vmin),pulsatile index(PI)and resistant index(RI)of the bilateral vertebral arteries(VA)and basilar arteries(BA)were examined by transcranial doppler sonography(TCD).The values of whole blood apparent viscosity and plasma viscosity of both groups were recorded.The indexes before and after treatment of both groups were compared.Results: The therapeutic effects of the above-mentioned indexes of the treatment group were significantly superior to those of the control group(P<0.05).In comparison with pre-treatment,the whole blood apparent viscosity(high,medium and low shear rates)and plasma viscosity of the treatment group post-treatment were obviously reduced(P<0.01,P<0.05).Conclusion: Needle pricking therapy combined with pulling-rotating manipulation can significantly improve VATCS patients' clinical symptoms,which may be closely related to its effects in lowering vascular blood resistance and blood viscosity and increasing cerebral blood supply.

AIM:To determine chlorogenic acid and hesperidin in Qingrehuoxue Mixture (Herba Artemisiae Scopariae, Pericarpium Citri Reticulatae, etc.) at the same time by HPLC. METHODS: A gradient method was set up with eluent of acetonitrile-water (0.4% phosphric acid). The detection wavelength of hesperidin was set at 283 nm, the detection wavelength of chlorogenic acid at 325 nm. RESULTS: The average recovery of two components were all above 95%, RSD was no more than 1.6%. The calibration curve was linear. CONCLUSION:The method is accurate and reliable with good reproducibility.

Mycelial morphology are exploited a great influnence to the metabolites of fungi. The effect of the mycelial morphology on producing lycopene by Blakeslea tripora was investigated. The results indicated that pellets has little role on fermentation when adding kerosene and triton-x100, However, The content of lycopene in the medium reached to 98.6mg/L by adding Ig/L span-20, forming dispersed mycelia, which is 3 times of the control experiments.

Objective To explore the relationship of post-transplant major histocompatibility complex class I chain-related gene A(MICA)antibody status and renal allograft function in clinical stable phase.Methods Fifty-seven patients accepted renal allografts followed up for at least 6 months were detected with the levels and specialties of MICA antibodies by Flow PRATM beads.Simultaneously,their serum ereatinine levels were tested as well.The impact of MICA antibody status on renal allograft function was assessed.Results Among the 57 patients,38 cases showed no HLA and MICA antibody.11 cases had HLA antibodies but not MICA antibody,8 cases had MICA antibodies and 3 cases had both MICA and HLA antibodies.There were 5 patients with MICA019 antibodies.3 patients with MICA027 antibodies,2 patients with MICA018 antibodies,while 1 patient with MICA004 and MICA017 antibodies,respectively.There were 9 patients with antibody positive score higher than 6,accounting 75%(9/12).Except age,there was no significant difference between patients with positive and negative MICA antibodies in the aspects of blood transfusion history,CDC,and cold ischemia time(P＞0.05).The average ages were(32.5±7.9)years for MICA antibodypositive patients and were(43.0±1 0.4)years for MICA antibody-negative patients(P=0.008).MICA antibody-positive patients without HLA antibody had higher serum creatinine level[(117.20±12.30)μmol/L]than MICA and HLA antibody-negative patients[(89.40±28.95)μmol/L,P＜0.05].Conclusions The measurement of MICA antibodies has prognostic value in the assessment of patients without HLA antibodies after renal transplantation.MICA antibody positive has clear association with chronic renal allograft function decline.

Objective To measure and define the distribution of left gastric lymph nodes.Meth- ods From Jan.2004 to Apr.2005,silver clips were set around the root of the left gastric artery in 124 pa- tients with esophageal and gastric cardia carcinoma,X-ray films at 0?and 90?simulator gantry in the radio- therapeutic position were taken.Then,the data of the superior,lower,left,right,anterior and posterior bor- der in each patient was recorded.With SAS 8.02 software,data of minimum area which covered the left gas- tric lymph node in different incidences were obtained.Results According to the analysis of Shapiro- Wilk,Kolmogorov-Smimov,Cramer-von Mises and Anderson-Darling tests,each border was of normal distri- bution,with equal frequency in the male and female,despite the actual results in different genders.Pearson Correlation Coefficients analysis did not suggest a significant relationship between the border and height, weight and size of vertebrae,which formed the minimum area covering the left gastric area at frequency of 100%,95%,90% and 85%,which were drawn out through the calculation.Conclusions Aiming at completely identifying the normal distribution of the left gastric lymph node,more patients are required to be in the pool.For the time being,location in the left gastric area can be obtained from details of the results in the present study.

The chemical constituents of Safflower injection were isolated and purified by polyamide, silica gel, Sephadex LH-20, ODS column chromatographies and preparative HPLC. As a result, sixteen compounds have been isolated. Based on the spectral data analysis, their structures were elucidated as scutellarin (1), kaempferol-3-O-β-rutinoside(2), hydroxysafflor yellow A(3), rutin (4), coumalic acid(5), adenosine(6), syringoside(7), (3E)-4-(4'-hydroxyphenyl)-3-buten-2-one(8), (8Z)-decaene-4, 6-diyne-1-Oβ-D-glucopyranoside(9), 4-hydroxybenzaldehyde (10), (2E, 8E) -tetradecadiene-4, 6-diyne-1, 12, 14-triol-1-O-β-D-glucopyranoside (11), kaem-pferol-3-O-β-sophorose (12), uridine (13), roseoside (14), cinnamic acid (15), and kaempferol (16). Compounds 1,2,7,9,11 and 12 were isolated from the Safflower injection for the first time. The anti-platelet aggregation activities of the isolated compounds were assayed. The results indicated all tested compounds exhibited potent activity except for 5, while 2, 3, 9 and 12 showed strong activity against platelet aggregation.
Animals ; Blood Platelets ; drug effects ; physiology ; Carthamus tinctorius ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; pharmacology ; Fibrinolytic Agents ; chemistry ; isolation & purification ; pharmacology ; Molecular Structure ; Platelet Aggregation ; drug effects ; Rabbits ; Spectrometry, Mass, Electrospray Ionization

This study was designed to investigate whether adenosine A1 receptors could modulate primary rhythmical respiration in mammals. Experiments were performed in in vitro brainstem slice preparations from neonatal rats. These preparations included the medial region of the nucleus retrofacialis (mNRF) with the hypoglossal nerve rootlets retained. The activity of the inspiration-related neurons (I neurons) in mNRF and respiratory rhythmical discharge activity (RRDA) of the hypoglossal nerve rootlets were simultaneously recorded by using microelectrodes and suction electrodes, respectively. Possible roles of adenosine A1 receptors in rhythmical respiration were investigated by administration of adenosine A1 receptor agonist R-phenylisopropyl-adenosine (R-PIA) and its specific antagonist 8-cyclopentyl-1,3- dipropylxanthine (DPCPX) into a modified Kreb's perfusion solution (MKS). DPCPX induced a significant decrease in the expiratory time and the respiratory cycles, and an increase in the discharge frequency and peak frequency of I neurons in the middle phase of inspiration. However, R-PIA significantly decreased the inspiratory time and integral amplitude as well as prolonged respiratory cycle. Moreover, the discharge frequency and the peak frequency of I neurons were decreased in the middle phase of inspiration, but not in the initial and terminal phases. The effect of R-PIA on rhythmical discharges could be partially reversed by additional application of DPCPX. These results indicate that adenosine A1-receptors are possibly involved in the modulation of rhythmical respiration through the inhibitory synaptic input from I neurons.
Animals ; Animals, Newborn ; Brain Stem ; physiology ; In Vitro Techniques ; Periodicity ; Rats ; Rats, Sprague-Dawley ; Receptor, Adenosine A1 ; physiology ; Respiration ; Respiratory Center ; physiology

Objective To investigate the effect of glutamine (Gln) on the content of reduced glutathione hormone (GSH) and aminoglutaminic acid (Glu) of spinal cord following ischemia-reperfusion injury. Methods Totally 40 healthy adult male rabbits were randomly divided into five groups: sham-operation group (S group), ischemia-reperfusion injury group (I/R group), low-dose glutamine group (L Gln group), median-dose glutamine group (M Gln group) and high-dose glutamine group (H Gln group). After glutamine preconditioning, the model of spinal cord ischemia-reporfasion injury was established according to Zivin's method. The general status of animals was observed and the changes of Jacobs scoring were recorded in each group. Malondialdehydes (MDA), GSH, Glu and superoxide dismutase (SOD) activity in lumbar spinal cord tissues were determined using chemical colorimetry. The neuron number and deviation rate in spinal cord anterior horn were observed histopathologically. Results There was no significant difference between L Gin group and I/R group in behavior scoring, SOD activity, content of MDA and Glu, neuron number and deviation rate of spinal cord (P>0.05); however, there was a significant difference in GSH content of spinal cord (P<0.05). M Gln group and I/R group differed significantly (P<0.05) in behavior scoring, SOD activity, content of MDA, Glu, GSH, neuron number and deviation rate of spinal cord. Between H Gln group and M Gln group, there was no significant difference in behavior scoring, content of MDA and Glu, SOD activity, neuron number and aberration rate in spinal cord (P>0.05), whereas there was a significant difference in SOD activity and Giu content (P<0.05). Conclusion Pretreatment with medium-dose glutamine has a protective effect on spinal cord ischemia-reporfasion injury in rabbits, which may be related to the maintenance of GSH content, increase of SOD activity and reduction of MDA.

Objective To study the influence of human leucocyte antigen(HLA) and major his-tocompatibility complex class Ⅰ chain-related gene A (MICA) specific antibodies on renal allograft function and graft rejective reaction by monitoring their changes from preoperative to postoperative pe-riods. Methods Twenty-seven patients with renal aliografts were tested with the specificity of anti-HLA antibodies (anti-HLA class Ⅰ and anti-HLA class Ⅱ) and anti-MICA antibodies and their posi-tive value changes by flow PRATM beads. The HLA genotype was integrated to distinguish donor specific antibody(DSA) and non-donor specific antibody(NDSA). Their serum creatinine levels and clinical data were analyzed simultaneously. Results Of the 27 patients, 22 cases accepted renal transplantation from dead bodies and 5 eases accepted from live donors. Except 1 failed patient, the other 26 patients had good functional renal allografts. Twenty-four survival patients were followed up on month 1, 3, 6 and 12 after transplantation. Seven out of 27 patients had pre-exist antibody before transplantation. Among them, 2 patients had anti-HLA antibody; 3 patients had anti-MICA antibody; 2 patients had both anti-HLA and anti-MICA antibody. Three patients with no anti-HLA and anti-MICA antibodies before transplantation created antibodies after transplantation from 3 to 6 months. One patient created NDSA after transplantation and appeared chronic rejection. There were 3 patients who had anti-MICA antibodies before transplantation. The expression levels of antibodies had changed from high to low, but the specific anti-MICA antibody had not changed during the follow-up on month 1, 3, 6 and 12 after transplantation. The patient with pre-transplantation low level of anti-HLA class Ⅱ antibody appeared acute rejection with fever and his CMV was positive as well. The patient's SCr levels changed from 171 μmol/L to 236 μmol/L after I to 3 months post-transplantation. Twenty-four patients were divided into positive and negative groups according to the specific antibody. There was significant difference of SCr levels between the 2 groups 1 month and 1 year after transplantation(P= 0.03, 0.05). Conclusions It is important to detect the specificity and positive value of anti-HLA antibodies and anti-MICA antibody regularly during the post transplantation follow-up. This will make an effective therapy for decreasing the occurrenee and development of acute or chronic rejection and hy-pofunction on renal allograft.