Objective To explore the feasibility of applying laser scanning confocal microscopy to investigate the metastatic characteristic of tumor cells in vitro. Methods The confocal microscope combined with transwell assay was employed to observe the invasion and migration of the metastatic colon cancer ]ovo cells when stimulated by IL-8. Results The results show that the number of invasive lovo cells in the stimulated group was much higher than the unstimulated group (P<0.05). The migration way of lovo cells observed under the confocal microscopy was ameba like movement. These findings suggest that the metastatic ability of lovo cells stimulated by IL-8 was enhanced. Conclusion The metastatic characteristic of colon adenocarcinoma lovo cells is similar to the chemotactic movement of leukocytes.

Cholesterol is an essential nutrient for human body.Dietary cholesterol comes from animal sources,including eggs,pork and others.A number of epidemiological and experimental studies showed that too much intake of cholesterol or consumption of more than 1 egg per day would slightly elevate blood cholesterol level.Results of studies in different areas and populations suggested that there is a lack of sufficient evidence to support the association between cholesterol intakes or egg consumption and cardiovascular disease incidence or deaths.

OBJECTIVE:To observe the application of sevoflurane combined with different dose of fentanyl in general anesthesia-inducing endotracheal intubation in adults. METHODS:60 selective patients were randomly divided into three groups(n=20). In haled 8% sevoflurane inductal anesthesia with tidal volume method. When the patients breathing and his own,and loss of eyelvaisah i .rve.f lTehxe, SgBroPu,p DⅠBwP earne dg iHvRen in2 e?agc?hk ggr-o1 ufpe nwtaenrye l dveitae cit.evd., agt rtoimupe Ⅱof 3lo s?sg-? okf-g -ey1 efleanstha nryelf lvexia ain.vd. baenfdo rger oinudpu Ⅲcti o4n ?agn?esktgh-e1s ifae(ntTan0)y,l before intubation(T1),at the moment of intubation(T2),at 1 min after intubation(T3),3 min after intubation(T4),5 min after intubation(T5)and 10 min after intubation(T6). RESULTS:Compared with at T0,SBP,DBP and HR in three groups were significantly decreased at T1(P0.05). SBP, fDeBntPa naynld i sH eRff einc tigvreo utop iⅢnd uwceer ea ndeescthreeassiae di nm aadrukletd elyn daot trTa(2chPe

Acute Coronary Syndrome ; metabolism ; pathology ; physiopathology ; Cholesterol ; metabolism ; Erythrocyte Membrane ; metabolism ; Humans

In this study,we observed that PGE2 3?10-7~3?10-6 mol/L & nifedipine 1 ?10-5mol/L inhibited lymphocyte proliferation markedly.The effect of nifedipine was completely reversible, however the effects of PGE2(3 ?10-6mol/L)was only partly reversible .In combination of PGE2(3?10-8mol/L) with nifedipine (1 ?10-6mol/L) ,the effect was additive,no significant antagonism and synergism were observed .PGE26? 10-6mol/L and nifedipine 1?10-3 mol/L directly inhibited maerbphage migration but no significant effect on PHA-induced MIF .Preincuba-tation with nefidipine did not influonce macrophage phagocytosis, but PGE2 slightly enhanced it. The results stated above strongly suggests that PGE2 may regulate cells interaction and Ca2+ is required during lymphocyte proliferation.

Objective:To explore the correlation of stress, coping style and the concentration of dehydroepiandrosterone sulfate in umbilical blood between preterm birth and normal birth.Methods:46 with preterm birth and 42 normal birth controls were assessed with Life Event Scale (LES) and Defense Style Questionnaire (DSQ) at 28th-week of pregnancy. The concentrations of DHEA-s in umbilical blood were determined by ELISA for the two groups. Results:Of the scales of malignant life events (frequency: 1.24?0.74 vs 1.04?0.03; strength: 56.21?4.03 vs 44.35?1.06)、immature and middle type defense style scales (4.24?0.13 vs 3.55?0.11; 3.86?0.08 vs 3.64?0.06), and DHEA-s (0.72?0.02 vs 0.33?0.03) there were significant difference between preterm birth and normal birth controls (P

Objective To investigate the predictive value of symmetrical ambulatory arterial stiffness index (S-AASI) in detecting early renal impairment of patients with essential hypertension.Methods Totally 245 consecutive out-patients were confirmed with essential hypertension,and were divided into group A (56 cases),group B (64 cases),group C (72 cases),and group D (53 cases) according to the quartile of S-AASI.The combination testing of serum cystatin C,serum β2-microglobulin as well as urine microalbumin to creatinine ratio were implemented as laboratory diagnosis index of renal impairment in early stage and 109 essential hypertension patients were diagnosed with early renal impairment.The parameters were compared among 4 groups.Pearson correlation analysis and partial correlation analysis were performed to confirm the relationship between the markers of early renal impairment and S-AASI.The predictive value of S-AASI to detect early renal injury was evaluated by analyzing Receiver Operating Characteristic (ROC) curve.Results With the rising of S-AASI,age as well as 24 hours mean systolic blood pressure (24 h SBP),serum cystatin C,serum β2-microglobulin,urine microalbumin to creatinine ratio and the incidence rate of early renal injury went notably higher while estimated glomerular filtration rate (eGFR) decreased significantly.After controlling for age,correlation test showed S-AASI was positively correlated with24hSBP,serum cystatin C,serumβ2-microglobulin,urine microalbumin to creatinine ratio(r =0.392,0.627,0.514 and 0.643 respectively,P ＜ 0.05) and negatively correlated with eGFR(r =-0.312,P ＜ 0.05).The 24 hours mean diastolic blood pressure (24 h DBP) was uncorrelated with S-AASI.Area under ROC curve of S-AASI for diagnosis of hypertensive renal impairment was 0.885.The critical value of S-AA-SI was 0.17,the sensitivity,specificity,positive predictive value,and negative predictive value were 92.7％,65.2％,68.5％,and 91.7％,respectively.Conclusions When S-AASI was detected above 0.17,patients with hypertension had a higher risk of renal impairment.Higher S-AASI was correlated with worse early renal impairment laboratory indexes.The predictive accuracy of S-AASI for early hypertensive renal impairment was medium.

OBJECTIVE:To screen the matrix formulation of Indomethacin hydrophilic gel patch. METHODS:Using appear-ance,adhesion,softness,moisture retention as indexes,mixed level of uniform design was used to screen matrix formulation of patch,verification test and in vitro transdermal test were used to evaluate the patch quality. RESULTS:Optimized matrix formula-tion was as follow as PVA of 7.686 g,PVP of 9.662 g,glyceryl of 19.992 g,gelatin of 9.999 g,CMC-Na of 13.997 g,carbomer of 3.000 g,HPMC of 1.500 g. The prepared patch had smooth surface,uniform matrix,good color,strong adhesion,good soft-ness(water loss rate of 11.9%). It can be slowly released for 48 h. The in vitro transdermal release fitted the Higuchi model(R2=0.9869). CONCLUSIONS:Indomethacin hydrophilic gel patch is successfully prepared,which can slowly release drug with over-all good quality.

0.05).CONCLUSION:Neuromuscular blockade induced by single i.v.of Cisatracurium showed no gender difference.

Autophagy ; Liver Cirrhosis ; physiopathology