3.Relationship of cell membrane microparticles CD31 and CD54 with alcohol-induced avascular necrosis of the femoral head:study protocol for a randomized controlled trial
Yun YANG ; Haiyan FAN ; Jian HUANG ; Zhongping MA ; Zhifeng ZHANG
Chinese Journal of Tissue Engineering Research 2016;20(44):6667-6672
BACKGROUND:Cel membrane microparticles CD31 and CD54 lead to microvascular injury in the femoral head by mediating vascular inflammatory response, promoting blood clotting, affecting vasomotion and promoting vascular endothelial injury. Studies have verified that membrane particles play an important role in steroid-induced necrosis of the femoral head, but there is no studies concerning relationship between microparticles and alcohol-induced avascular necrosis of femoral head. METHODS/DESIGN:This is a randomized control ed animal study. Healthy male Wistar rats wil be randomly assigned to two groups. In the model group, rats wil be intragastrical y administered hard liquor for 6 consecutive months to prepare models of alcohol-induced avascular necrosis of the femoral head. Blank controls wil be intragastrical y given an equal volume of physiological saline. In 1-6 months of intervention, six rats wil be randomly selected from each group every month. Blood wil be col ected separately. Flow cytometry wil be used to detect serum cel membrane particles CD31, CD54 levels. Bilateral femoral head wil be fixed, decalcified, embedded in wax, and then sections. After hematoxylin-eosin staining, empty bone lacuna wil be quantified under a light microscope to identify femoral head necrosis. Verhoff’s staining and MSB microthrombosis staining wil be used to observe microvascular injury and microvascular thrombosis in the femoral head, and to analyze the correlation of CD31 and CD54 levels with femoral head necrosis, vascular endothelial injury and microvascular thrombosis. DISCUSSION:This study wil investigate the effects of CD31 and CD54 on alcohol-induced avascular necrosis of the femoral head, explore the pathogenesis of alcohol-induced avascular necrosis of the femoral head, provide a new theoretical basis for early diagnosis and early treatment, and may provide a new target for its treatment. ETHICS APPROVAL:The protocol has been approved by the Animal Experimental Ethics Committee of Inner Mongolia Medical University (approval number YKD2016154). Experimental procedures and materials of rats wil be in accordance with the Guide for the Care and Use of Laboratory Animals, which is consistent with the guide of National Institutes of Health. Subject headings:Femur Head Necrosis;Membrane Proteins;Tissue Engineering
4.Establishment of a rat model ofalcohol-induced avascular necrosis of the femoral head
Yun YANG ; Haiyan FAN ; Jian HUANG ; Zhongping MA ; Zhifeng ZHANG
Chinese Journal of Tissue Engineering Research 2016;20(27):3977-3983
BACKGROUND:The relationship between long-term heavy drinking and alcohol-induced necrosis of the femoral head has long been clear, but thepathogenesis of alcohol-induced necrosis of the femoral head is currently not fuly understood.
OBJECTIVE:To establish a rat model of alcohol-induced avascular necrosis of femoral head and to study its pathogenesis.
METHODS: Eighty Wistar rats were randomly divided into experimental and control groups (40 rats per group). Rats in the experimental group were intragastricaly administered strong wine 10 mL/kg, once a day, for 6 consecutive days. Rats in the control group were given physiological saline 10mL/kg, once a day, for 6 consecutive days. Bilateral femoral heads were randomly colected from six rats every month for histomorphological observation.
RESULTS AND CONCLUSION:(1) Osteonecrosis: in the experimental group, at 3 months, trabecular bone became thin, arranged disorderly, and the number of empty lacuna began to increase. At 6 months, typical osteonecrosis appeared, and vacant lacunaes increased significantly. In the control group, trabecular bone was complete and neatly arranged. Osteocytes were visible in bone lacuna, and normal morphology of cels was seen. (2) Injury of blood vessels: in the experimental group, at 3 months, micro-intimal hyperplasia was observed. Elastic fibers of partial vascular endothelium were reduced. Elastic fiber andmiddle-layer smooth muscle breakage and proliferation were found. At 6 months, above manifestations were more remarkable. In the control group, arteriole film was not thickened, and vessel wal was normal. (3) Formation of microthrombus, in the experimental group, the number of microthrombus was increased at 3 months, and became significant at 6 months. In the control group, the number of microthrombus was not altered. (4) Results indicated that chronic alcohol intake can lead to microvascular endothelial injury in the rat femoral head. Abnormal blood microcirculation was detected in local region, and resulted in avascular necrosis of the femoral head. The degree of necrosis was associated with alcohol intake.
5.DICE regimen for patients with relapsed or chemo-resistant invasive non-Hodgkin lymphoma
Lin TAN ; Yun ZENG ; Yu XIE ; Jian YANG
Journal of Leukemia & Lymphoma 2009;18(1):32-34
Objective To evaluate the efficacy of DICE regimen on relapsed or refractory NHL,and observe its toxicitv.Methods Records of 50 patients with relapsed or refractory invasive NHL were treated with DICE regimen.All patients had received at least 1 type of chemotherapy regimen with a median of 6 cycles.The patients received a median of 4 cycles of DICE regimen.Resuits The treatment outcome and adverse events of all patients were analyzed.The overall response rate was 48.0%.with a complete response (CR)rate of 16.0%.The response rates were 53.8%in the 26 patients with B-cell lymphoma and 29.2% in the 24 patients with T-cell lymphoma.The 1-and 2-year survival rates were 34.0%and 8.0%,respectively.The major adverse event was myelosuppression:the prevalence of grad eⅢ-Ⅳ neutropenia Was 38.0%,and that of grade Ⅲ-Ⅳ was 14.0%.One patient suffered grade Ⅲ liver toxicity.Conclusion DICE regimen Was effective for patients with relapsed or refractory invasive NHL, and its toxicity is well tolerated,but the response term is relatively short.Further clinical study on the application of DICE regimen iS needed.
6.Study on Optimization of Soybean Meal Solid Fermentation Process by Response Surface Analysis
Jian-Feng LIU ; Xiang-Yang GE ; Yun-Xiang LIANG ;
China Biotechnology 2006;0(06):-
Response surface analysis (uniform precision of central composite design, SAS 9.1.3 software) was applied to optimize the four major factors (ratio of soybean meal to water, enzyme quantity, fermentation time and inoculation quantity) for soybean meal solid fermentation. According to the change of the hydrolyzation degree of soybean protein, the equation of polynomial regression was established between those factors and the response. The result showed that the optimum condition included as follows: ratio of soybean meal to water 1∶1.00,enzyme quantity 2.55%, fermentation time 65h and inoculation quantity 1.00%. Under the optimum level, the degree of hydrolyzation reached 13.3%, which increased 56% over pre-optimization.
7.Synthesis of glycylproline p-nitroanilide tosylate and its clinical application
li-yun, YAO ; jian-hua, ZHANG ; rong, WANG ; yang, LU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(04):-
Objective To synthesize glycylproline p-nitroanilide tosylate as a substrate for glycylprolyl dipeptidyl-aminopeptidase(GPDA),and to study its clinical application.Methods The title compound was prepared by DCC-HOBt synthesis.GPDA in human serum was detected by continuous monitoring method using substrate. Results The substrate with purity of 98.5% was obtained.The linearity of the method was up to 358.1 U/L.Intraassay CV and interassay CV of GPDA in diverse serum samples were 3.01% and 5.04%,respectively.It was shown that GPDA level in patients with hepatic cancer was significantly increased,while those in patients with gastric carcinoma and chronic gastritis were significantly decreased compared with that in the control group in clinical detecting(P
8.Correlation of acute ischemic cerebral infarction with carotid atherosclerosis plaque
Min WU ; Xiaoyan XIN ; Jian YANG ; Yun XU
Journal of Medical Postgraduates 2003;0(08):-
Objective:Atherosclerosis of the carotid artery is the main cause and risk factor of ischemic cerebral infarction.We aim to evaluate the relation between acute ischemic cerebral infarction and the carotid atherosclerosis plaque.Methods: The distribution,shape,number and echoic features of the carotid atherosclerosis plaques confirmed by MRI diffusion-weighted imaging(DWI) were analyzed by color Doppler ultrasonography in 90 patients with acute ischemic cerebral infarction(Group A) and 82 controls(Group B).Results: The positive rate of acute cerebral ischemic infarction was significantly higher in Group A(76.7%) than in Group B(32.9%,P
9.A robust statistical procedure to discover expression biomarkers using microarray genomic expression data.
Yang-yun ZOU ; Jian YANG ; Jun ZHU
Journal of Zhejiang University. Science. B 2006;7(8):603-607
Microarray has become increasingly popular biotechnology in biological and medical researches, and has been widely applied in classification of treatment subtypes using expression patterns of biomarkers. We developed a statistical procedure to identify expression biomarkers for treatment subtype classification by constructing an F-statistic based on Henderson method III. Monte Carlo simulations were conducted to examine the robustness and efficiency of the proposed method. Simulation results showed that our method could provide satisfying power of identifying differentially expressed genes (DEGs) with false discovery rate (FDR) lower than the given type I error rate. In addition, we analyzed a leukemia dataset collected from 38 leukemia patients with 27 samples diagnosed as acute lymphoblastic leukemia (ALL) and 11 samples as acute myeloid leukemia (AML). We compared our results with those from the methods of significance analysis of microarray (SAM) and microarray analysis of variance (MAANOVA). Among these three methods, only expression biomarkers identified by our method can precisely identify the three human acute leukemia subtypes.
Biomarkers
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Gene Expression Profiling
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Humans
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Leukemia, Myeloid, Acute
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classification
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diagnosis
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Monte Carlo Method
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Oligonucleotide Array Sequence Analysis
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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classification
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diagnosis
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Statistics as Topic
10.Study on inhibitory effect of calycosin on hepatic stellate cell activation in rats by up-regulating peroxisome proliferator-activated receptor γ.
Jian PING ; Hong-yun CHEN ; Yang ZHOU ; Gao-feng CHEN ; Lie-ming XU ; Yang CHENG
China Journal of Chinese Materia Medica 2015;40(12):2383-2388
To observe the effect of calycosin on the proliferation and activation of primary hepatic stellate cells (HSCs) in rats, and prove calycosin shows the effects through peroxisome proliferator-activated receptor γ(PPARγ) and farnesoid X receptor (FXR). The results indicated that calycosin could inhibit HSC proliferation and expressions of activation marker smooth muscle actin-α and type I collagen. With the increase in HSC activation time, FXR expression reduced, but with no notable impact from calycosin. Calycosin could up-regulate PPARγ expression and its nuclear transition in a concentration-dependent manner. Its prohibitory effect on HSC activation could be blocked by PPARγ antagonist. In conclusion, calycosin could inhibit HSC activation and proliferation, which may be related with the up-regulation of PPARγ signal pathway.
Animals
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Cell Proliferation
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drug effects
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Cells, Cultured
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Drugs, Chinese Herbal
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pharmacology
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Hepatic Stellate Cells
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cytology
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drug effects
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metabolism
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Isoflavones
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pharmacology
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Male
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PPAR gamma
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genetics
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metabolism
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Rats
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Rats, Sprague-Dawley
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Receptors, Cytoplasmic and Nuclear
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genetics
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metabolism
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Up-Regulation
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drug effects