Objective The aim of this investigation is to explore the effects of hypoxia on the biological activity of endothelial progenitor cells(EPC)and to improve the efficacy of EPC transplantation.Methods Rat bone marrow derived EPC were isolated and cultured either under normoxic or hypoxic conditions.The proliferation,migration and angiogenic ability of EPC were observed.Results In hypoxic group,the number of attached cells per high power field(hpf)was significantly more than that in normoxic group (91.0?8.0)vs(42.5?5.3),P

Bone marrow mesenchymal stem cells (BMSCs) are a kind of pluripotent stem cells derived from bone marrows, which can not only support hematopoiesis, but also have capabilities of multidifferentiation, high-proliferation and self-renewing. They have become one of hotspots in stem cell studies. Studies on in vitro intervention with BMSCs with TCMs have made remarkable progress in recent years. According to the findings, some traditional Chinese medicines can promote proliferation of BMSCs, some can inhibit the apoptosis of BMSCs, while others can induce BMSCs to differentiate into multiple cell types, such as osteoblast. Furthermore, some studies also involved relevant action mechanisms. The authors summarized the advance in relevant studies by reference to relevant literatures of this field.
Animals ; Apoptosis ; drug effects ; Bone Marrow Cells ; cytology ; Cell Differentiation ; drug effects ; Cell Proliferation ; drug effects ; Humans ; Medicine, Chinese Traditional ; methods ; Mesenchymal Stromal Cells ; cytology ; drug effects

Animals ; Feeding Behavior ; Male ; Rats ; Rats, Sprague-Dawley ; Taste ; Viscera ; pathology

OBJECTIVETo investigate the clinical effects of anterior cervical intervertebral space decompression under microscope in treating cervical spondylotic myelopathy in elderly patients.

METHODSFrom June 2009 to March 2012, 43 patients with cervical spondylotic myelopathy were treated with anterior cervical intervertebral space decompression and intervertebral fusion under microscope. There were 26 males and 17 females, aged from 60 to 72 years old with an average of (64.9±3.7) years. Japanese Orthopaedic Association System (JOA) score was from 7 to 12 points with an average of (9.5±1.8) points before operation. The function of nerves was assessed before and after operation according to JOA.

RESULTSAll patients were followed up from 10 to 18 months with an average of (14.7±1.6) months. Postoperative JOA score was (13.81±1.44) points (ranged, 10 to 16), had significantly higher than preoperative (P<0.01). According to the rate of the improved JOA score, 9 cases got excellent results, 26 good, 7 fair, 1 poor.

CONCLUSIONAnterior cervical intervertebral space decompression under microscope for cervical spondylotic myelopathy in elderly patients is safe and effective.

Aged ; Cervical Vertebrae ; surgery ; Decompression, Surgical ; methods ; Female ; Humans ; Male ; Microscopy ; Middle Aged ; Spondylosis ; surgery

OBJECTIVETo seek an effective drug for treatment of human hyperplastic scar through studying the effects of curcumin on fibroblast growth and collagen synthesis.

METHODSFibroblasts derived from scar tissue and from normal epidermal tissue were isolated and cultured separately with tissue-block method, their morphology were observed under invert phase contrast microscope, their growth curve was drawn respectively to determine the speed of growth. Then, fibroblasts from scar were stimulated with curcumin in different concentrations (0, 12.5, 25, 50 and 100 micromol/L) for detecting the inhibitory effect of curcumin on growth of fibroblasts using MTT methods and that on activity of procollagen alpha-1 gene transcription in fibroblast was detected by RT-PCR.

RESULTSThe cell growth curve showed that double-multiplying time was 5 days in fibroblasts from scar and 4 days in those from normal dermis, showing significant difference between them (P < 0.05). MTT showed that curcumin in 12.5 micromol/L showed a cell proliferation enhancing trend, and its absorbance value was significantly higher than that in the normal group, but the effect turned to inhibition when concentration increased to over 25-100 micromol/L, and became significant inhibition at concentration of 50 and 100 micromol/L. Besides, curcumin also showed markedly inhibition on collagen type I synthesis in fibroblasts (P < 0.01).

CONCLUSIONHigh concentration curcumin can inhibit effectively the fibroblast proliferation and collagen I synthesis in hyperplastic scar, therefore, may has therapeutic effect on the disease in human being.

Cell Proliferation ; drug effects ; Cells, Cultured ; Cicatrix, Hypertrophic ; metabolism ; pathology ; Collagen Type I ; biosynthesis ; Curcumin ; pharmacology ; Fibroblasts ; drug effects ; metabolism ; pathology ; Humans

Objective To explore the efficacy and safety of the resection of prostate-tangerine techniquewith 980 nm diode laser benign prostatic chyperplasia(BPH).Methods Data of 60 patients with highvloum BPH treated with the resection of prostate-tangerine technique with 980 nm diode laser were reviewed.The age of the patients were 65-89 years old,and mean prostate volume was 74.5± 13 ml.The mean operative time,blood loss were oberseved and recored,the international prostate symptom score(IPSS),quality of life score (QOL),maximum urinary flow rate(Qmax) and residual urine volume (PVR) were collected and analyzed.Results The 60 cases were safe during the perioperative period.The average operation time was (70.2± 16.9) min,and there was no obvious bleeding during the operation and no blood transfusion cases.The time of remove the transurethral catheter was 2-5 d,and the mean time was (2.4±0.3) d.Follow up 1-3 months after operation,a significant reduction in IPPS was reported (P =0.000),significant durable improvements in Qmax,PVR before operation(P=0.000).Conclusion Transurethral vaporization of 980 nm diode laser could be a safe and effective treatment modality for BPH.

An oxygen-tolerant denitrifying strain designated as H1 was screened by the procedures of shallow shaking and continuous aeration cultures.With the aid of an nnrS-gfp fusion responsive to nitric oxide (NO)and acetylene inhibition-GC procedure,it was shown that strain H1 was able to produce NO and N_2O but not N_2 under denitrifying conditions.Denitrifying processes were thus determined as NO_3~-→NO_2~-→NO→N_2O,with N_2O as the end product.Strain H1 could denitrify under shallow shaking conditions as well as in the initial atmospheric oxygen concentration ranging from 0～21%.Denitrification processed normally under continuous aeration at the rate of 2 L air per min in a 150 mL medium,but stopped under high aeration rate as 5 L air per min.16S rRNA gene sequence revealed that strain H1 shared 98% similarity to its closet relative Ralstonia taiwanensis,the genus where denitrifying bacteria are frequently found.

Objective To explore the operative methods and clinical outcomes in emergency or sube- mergency repair for the complicated tissue defects in hand in first stage applying microsurgical technique. Methods From Jan.,2000 to Aug.,2005,49 emergency cases of complicated hand tissue defects were re- paired in the first stage with replantation,reconstruction,free flaps,combined finger reconstruction and flap transplantation,including 21 cases in mini tissue mass replantation or reconstruction,15 cases in replantation combined with free flap transplantation,8 cases in replantation and reconstruction combined with free flap transplantation,5 cases in combined multiple digits reconstruction with free flap transplantation.The free flap transplantation included the anterolateral femoral flap,the latissimus dorsi myocutaneous flap,the dorsalis pe- dis flap,the media pedis flap and the instep flap.Results All the flaps,the replanted and reconstruceted finger survived uneventfully except for one replanted finger necrosis.45 cases healed in the first stage and the other 4 cases healed in the second stage.During a follow-up from 6 months to 3 years postoperatively,a satis- factory appearance and function of the reconstructed hand was achieved.The excellent and good rate was 85.7% assessed with provisional functional assessment criterion for upper limbs issued by Chinese Society of Hand Surgery.Conclusion The emergency or subemergency repair for the complicated tissue defects in hand has the advantage of short-term treatment and desirable functional outcome.The emergency replantation and reconstruction combined with various flaps or tissue mass can be applied to repair tissue defect in hand in the first stage according to the position and area of the defect along with the technique level of the surgeon, having been proved to achieve desirable clinical outcomes.And the key points leading to a successful operation is the correct treatment for the raw surface of the defects,suitable choice for various flaps,logical design of combination pattern and prevention and timely treatment for vessel crisis.

AIMTo investigate the effects of central amygdaloid nucleus (CeA) on the evaluation of taste in rats, and explore the mechanisms of the CeA in modulating the feeding behavior.

METHODSBy using two-bottle choice test, we measured the consumption of serials concentrations of NaCl, citric acid (CA), quinine HCl (QHCl) and sucrose in bilateral CeA lesioned rats, and compared the results to those in sham lesioned rats.

RESULTSThe CeA-lesioned rats exhibited a lower preference for NaCl at concentrations of 0.03; 0.1 and 0.3 mol/L, for CA at concentrations of 0.01; 0.1 and 1.0 mmol/L, and for QHCl at 10; 20 and 50 micromol/L, but the preference for serials of concentrations of sucrose are similar between two groups. By comparing the intake of adjacent concentrations of sapid solutions, it indicated that CeA-lesioned rats showed a lower distinction between adjacent concentrations of NaCl, CA and QHCl. However, the total consumption (water and tastants) during all the test sessions was not significantly different between two groups of rats.

CONCLUSIONLesion of CeA decreases the intake of tastants solution, but the effects on different concentrations of solution are different. It suggests that the CeA plays an important role in the normal response to exteroceptive food stimuli through impacting on the assessment of taste and altering the preference threshold of gustatory stimuli.

Amygdala ; physiopathology ; Animals ; Drinking ; Electric Stimulation ; Feeding Behavior ; Male ; Pons ; Rats ; Rats, Sprague-Dawley ; Taste ; physiology

With the development of cerebral interventional medical devices, Nitinol alloy has been widely used in clinical fields as a good biomaterial. This essay is to make brief comments on Nitinol alloy's present development, its material characteristics, medical basic researches, and applications in cerebral interventional devices.
Alloys ; Biocompatible Materials ; Cerebral Revascularization ; instrumentation ; Humans ; Stents