extraction time.The extraction of total flavonoids in health food was the highest under the situations of ethanol concentration 60%,solid-to-liquid ratio 1:20 and ultrasonic time 20 min.The average value was 5.370 mg.The linear range of total flavonoids was between 1.0-40 ?g/ml while the regression equation is y=0.003 3x-0.010 9,correlation coefficient was 0.999 5 and detection limit was 1.0 ?g/ml.The standard addition recovery rates of this method were 95%-104%(n=3),RSD was 1.717%(n=5).Conclusion Under the optimum situation,this method has high precision and accuracy while the blank is low and stable as well.It is applicable to the detection of total flavonoids in health food and some other foods.

Because of spine structure and location importantance, spine artificial surgery is not only high-risk but also require proficiency of doctors. Image navigation system for spinal surgery could reduce risk and postoperative complications, and spinal image segmentation and registration methods are the key steps. This paper summarized spinal image segmentation and registration methods. Based on analysis of existing methods, we pointed out the shortcoming in the field, thus forecasts future development.

Currently praziquantel is one of the major drugs used in treatment of schistosomiasis and other trematode infections.Recent experimental studies indicate that a new anthelmintic,tribendimidine,is used in the treatment of intestinal nematodes,also possesses effect against several species of trematodes including Clonorchis sinensis,Opisthorchis viverrini and Echinostoma caproni.Tribendimidine is even more effective against C.sinensis in rats that a single 300 mg/kg oral dose cures almost all of the animals treated,a lower cure dose than praziquantel(375-500 mg/kg).The anti-malarial drugs artemether and artesunate are not only effective in the prevention of schistosomiasis,but also effective against several species of trematodes,especially C.sinensis.The single oral dose of both drugs to cure or achieve high efficacy in infected rats is 75 mg/kg.This review summarized research progress on tribendimidine,artesunate,and artemether in experimental animals infected with C.sinensis and other species of trematodes.

The measurement of pressure between tongue and the hard palate during swallowing is an evaluation method which is noninvasive and accurately quantitative. This article reviewed the measurement of tongue pressure, the changes of pressure in different poses,bolus types and people, as well as the prediction of dysphagia according to tongue pressure.

AIM: To explore the clinical effects of high frequency electrical capsulotomy in maturation period cataract surgery.
●METHODS: A total of 68 cases of maturation period cataract were selected and underwent the surgery of continuous circular capsulorhexis using the high frequency electrical capsulotomy.
●RESULTS: The success rate was 91% in 68 cases with the high frequency electrical capsulotomy.
● CONCLUSION: The high frequency electrical capsulotomy in maturation period cataract surgery has significant advantages and brilliant clinical values.

Objective To observe the changes of extracellular matrix(ECM) production and hypertrophy of glomerular mesangial cells(GMCs) induced by high glucose(HG),and the inhibitory role of myriocin(ISP-1).Methods GMC cultured with normal glucose(5.6 mmol/L D-Glucose,NG),HG(25.2 mmol/L D-Glucose) and HG plus ISP-1(100 mg/L) for different durations(0,24,48,(72 h)).The sizes of GMC were indicated by forward scatter intensity,measured by flow cytometery,and the levels of fibronection(FN),collagen Ⅳ(Col Ⅳ),laminin(LN),precollagen Ⅲ(Pcol Ⅲ) and hyaluronic acid(HA) in the supernatant of cultured GMC were detec-(ted) by ELISA.Results Compared with NG,HG could induce GMC hypertrophy(P

Objective To discuss the significance of the application of real-time quantitative PCR(RQ PCR)for diagnosis and guidance therapy of cytomegalovirus(CMV)infection after allo- hematopoietic stem cell transplantation(allo-HSCT).Methods Thirty-three patients were undergo- ing allo-HSCT.After hematopoietic reconstitution,patients'peripheral blood samples were detected for CMV DNA by RQ-PCR periodically.Anti-viral therapy was begun,attenuated and stopped ac- cording to the results of CMV DNA detection.The effects of anti-viral therapy and patients' clinical results were observed.Results CMV DNA was detected in blood samples from 13 of 33 patients. There were 21 episodes in these patients.Only 1 episode wasn't controlled hecause the patient gave up the therapy.CMV DNA copies were disappeared soon or decreased and then disappeared during anti-viral therapy in the others'.Patients which had symptoms and/or dysfunction of organs were cured too.Each course of anti-viral therapy was shorter than ordinary course.Conclusions CMV in- fection can he diagnosed as early as possible by RQ PCR.To begin,attenuate and stop anti-viral ther- apy according to the results of RQ-PCR is safe.The course is shortened and the side effects of anti vi- ral drugs were attenuated.

Background Corneal contact lens have been widely used in vitrectomy.The corneal ring accompanying with the contact lens therefore is also much concerned.We designed a sutureless silicone corneal ring to renew a suturing?ring,but its detection of physicochemical properties and hiocompatihility is necessary for clinical application. Objective This study was to investigate the physicochemical properties and biocompatibility of the newly designed sutureless silicone corneal ring. Methods The Shore hardness,tensile stress-strain tests,tear strength,transmittance and the haze of the novel silicone ring were tested.The bioeompatibility of sutureless silicone corneal ring to corneal epithelial cells was evaluated by determining cytotoxieity.The ring was implanted into the counjunctival soc of 6 Japanese white rabbits for 4 hours to determine its stimulation response by measuring the area of corneal fluorescence staining,and then the ring was placed on the skin surface of 6 SD rats for 4 hours to assess the presence of allergic reaction by calculating the swelling area of skin. Results The new sutureless silicone corneal ring showed good physiological features with a Shore hardness of 63.4 degree,tensile strength more than 5.86 MPa,elongation＞100%and tear strength 34 kN/m.The transmittance of 3 legs in the ring was more than 93%;however,the haze was less than 0.1%.Cytotoxicity of the ring was 3.1 1×10-6%.No obvious corneal fluorescence staining was seen at any time point in all the rabbit eyes in the stimulating test.Furthermore,there was not any skin swelling in various time points in all the SD rats in the allergy test. Conclusion The physicochemical properties of the silastic used in our newly designed sutureless silicone cornea ring can meet the demands of the ring.The biological evaluation complies with the ISO i0993-1 international standards and meets the demands of the organism.Thus,it is all ideal material to use in constructing the corneal ring.

Adult ; Antigens, CD20 ; metabolism ; Empyema, Pleural ; complications ; diagnostic imaging ; pathology ; virology ; Epstein-Barr Virus Infections ; Humans ; Ki-67 Antigen ; metabolism ; Lymphoma, Large B-Cell, Diffuse ; metabolism ; pathology ; surgery ; virology ; Male ; Pleural Neoplasms ; complications ; metabolism ; pathology ; surgery ; virology ; Radiography

Animals ; Brain ; metabolism ; Female ; Ginkgolides ; pharmacology ; Hypoxia ; metabolism ; Lactic Acid ; metabolism ; Male ; Malondialdehyde ; metabolism ; Rats ; Rats, Sprague-Dawley ; Sodium-Potassium-Exchanging ATPase ; metabolism