1.Sequelae of hypergastrinemia due to long-term administration of proton pump inhibitors and its management
Jian SHEN ; Jianxin WU ; Dingguo LI
Chinese Journal of Clinical Pharmacology and Therapeutics 2004;0(12):-
Proton pump inhibitor (PPI) is considered as the standard treatment for acid-related disorders. However, its long-term use, especially in patients with gastroesophageal reflux disease, would cause potential risks, such as hypergastrinemia along with reduced gastric acidity, hyperplasia of enterochromaffin cells (ECL), gastric neoplasms, rebound gastric acid hypersecretion when PPI treatment is stopped, increased oxyntic gastritis in patients with H. pylori infection, and the possible stimulation of growth of non-gastric tumours due to hypergastrinaemia. Each of these trends has led to numerous studies and evaluations on the potential risk-benefit ratio of the long-term use of PPIs, and countermeasures are being proposed for these problems.
2.The Effects of Arsenite Trioxide in Various Concentrations on the Esophageal Carcinoma Cell Line
Jian SHEN ; Minhua WU ; Weijia CAI ; Zhongying SHEN ;
Chinese Journal of Cancer Biotherapy 1995;0(02):-
Objective: To further study the effects of As 2 O 3 in various concentrations on the esophageal carcinoma cell line. Methods: The esophageal malignant transformed epithelial cells (SHEEC1) were induced by HPV18 in synergy with TPA in our laboratory. The cells cultured in flask and 24 wells plate were treated by As 2 O 3 with concentrations of 1, 3 and 5 ?mol/L for 2, 4, 8, 16, 24 h respectively. The morphologic changes of cells were observed under election microscopy. The mitotic index (MI) of living cells was calculated by phase contrast microscopy and the cells with TdR uptake were examined by autoradiography. The proliferative index (PI) and apoptotic index (AI) were assayed by flow cytometry. Results: A low dosage of As 2 O 3 (1.0 ?mol/L) enhancing the protiferative rate of SHEEC1 was demonstrated with TdR uptake, MI and PI increased. The high AI and low PI were found in the high concentrations (3 and 5 ?mol/L)of As 2 O 3 . The morphological changes of apoptosis and necrosis were found in 24 h after As 2 O 3 in high concentrations (3 and 5 ?mol/L) administrated. Conclusion: The effects of As 2 O 3 in various concentrations are different. Low concentration of As 2 O 3 promotes the proliferation of the esophageal carcinoma cells by increment of DNA synthesis. In high concentration of As 2 O 3 apoptosis and necrosis are induced.
3.The Effects of Arsenite Trioxide on the Transplanted Hepatic Carcinoma in Mice
Jian SHEN ; Minhua WU ; Minghua CHEN ; Qiaoshan LI ; Zhongying SHEN ;
Chinese Journal of Cancer Biotherapy 1995;0(03):-
Objective: To investigate the antitumor effects of arsenic trioxide (As 2 O 3 ) on solid tumor in vivo , the transplanted hepatic carcinoma of mice were treated with various dosages of As 2 O 3 . Methods: After 50 mice were injected intraperitoneally with 0,2, 4, 6, 8 ?mol/g As 2 O 3 respectively for observation of acute toxicity, 1, 2, 3 ?mol/g were selected for this experiment. Eighty mice were transplanted with hepatic carcinoma cells (1.5?10 6 cells/each side) into both subaxillae. As 2 O 3 in 0, 1, 2 and 3 ?mol/g were injected into right subaxilla respectively once every day for 10 days. At the 30th day after transplanted hepatic carcinoma cell, mice were killed and the size of solid tumors was measured. The chronic cytotoxicity of As 2 O 3 was observed in these mice.Results: In right subaxillae tumors, which were injected directly with As 2 O 3 , were apparent smaller than that in the left ( P
4.Clinical value analysis of serum protein fingerprint in patients with rheumatoid arthritis associated interstitial lung disease
Yanmei WU ; Shouchun PENG ; Jian SHEN ; Jing WU
Chinese Journal of Immunology 2015;(2):236-239
Objective:To screen differentially expressed proteins in serum proteomic patterns in patients with Rheumatoid Arthritis associated Interstitial Lung Disease by Surface enhanced laser desorption /ionization mass spectrometry with Protein Chip ,and evaluate the value of a preliminary study.Methods: WCX2 ( Weak cation exchange chip ) and Surface enhanced laser desorption/ionization mass spectrometry with ProteinChip were performed to test serum proteins in 19 Rheumatoid Arthritis associated Interstitial Lung Disease,15 Rheumatoid Arthritis patients and 13 health adults,Biomarker Wizard ,Biomarker Pattern and Spss13.0 software were used in combination to analyze the data.Results:The mass/charge ratio ( M/Z) value of 142 protein peaks were detected in serum , there were 4 proteins differentially expressed with statistical significance among them ( P<0.05;Student′s t-test ) , which were the proteins with M/Z 3382.59 ,3453.39 ,11886.0 ,5825.48;the proteins with M/Z 11689.4 ,2266.49 ,1020.22 ,4392.28 ,5074.38 and 2764.69 were selected to establish the optimal diagnostic model.The sensitivity was of was 90%(9/10),the specificity was of 90%(18/20).Conclusion: Surface enhanced laser desorption/ionization time of flight mass spectrum can screen out the significantly different proteins in serum of patients with Rheumatoid Arthritis associated Interstitial Lung Disease.As bomarkers , both of their sensitivity and specificity were high and determination have good prospects for clinical application .
5.Electro-acupuncture on the iNOS of Lumbar Intervertebral Disk Tissues in Rats
Bimeng ZHANG ; Yaochi WU ; Huangan WU ; Jian SHEN
Journal of Acupuncture and Tuina Science 2006;4(2):71-74,封二
Objective: The present study is to investigate the mechanism of electro-acupuncture in preventing and treating lumbar intervertebral disk displacement. Methods: Seventy-five Sprauge-Dawley rats were used to establish the models of lumbar intervertebral disk displacement by operation, and divided into five groups including electro-acupuncture group, western medicine group, acupuncture plus medicine group, model group and normal control group, then they were treated with corresponding therapeutic method. The expression of inducible nitric oxide synthase (iNOS) mRNA in the lumbar intervertebral disk tissues of rats was detected by RT-PCR. Results: The expression of iNOS mRNA in the lumbar intervertebral disk tissues of rats in model group was compared with that in normal control group, the significant difference was found (P<0.01); While the expressions of iNOS mRNA in the lumbar intervertebral disk tissues of rats in electro-acupuncture group, western drug group and acupuncture plus medicine group were all inhibited, and compared with the model group, the significant differences were also found (P<0.01). Conclusion: Electro-acupuncture could inhibit the expression of iNOS mRNA in the lumbar intervertebral disk tissue.
6.Transplantation of marrow mesenchymal stem cells improves the ventricular remodeling and heart functions to acute MI rat models
Xuxian WU ; Zhixu HE ; Xiangchun SHEN ; Zhongjun ZHOU ; Jian LIU
Basic & Clinical Medicine 2006;0(01):-
Objective To study the effects of marrow mesenchymal stem cells on heart functions and ventricular remodeling after myocardial infarction in rats. Methods Myocardial infarction model was established by ligation of the left anterior descending coronary artery (LAD) in adult SD rats. 4 and 8 weeks after MMSCs implantation,hemodynamic evaluations,left ventricular weight/body weight ratio and heart weight/body weight ratio were determined. HE staining was performed for counting microvasculars and Van Gieson staining was used for measurements and calculation of the myocardial fibrillar collagen. Then we investigated the migration and evolution of MSCs in vivo by fluorescent microscope. Results HR was significantly decreased in transplantation MMSCs group. Eight weeks after transplantation,body weight in transplantation MMSCs group reached that of control group. At the same time,SBP,DBP and MBP were significantly increased in transplantation MMSCs group. HR was significantly decreased in transplantation MMSCs group. Secondly,left ventricular weight/body weight ratio and heart weight/bodyweight ratio were significantly decreased 4 weeks after transplantation MMSCs. Then the ratio was significantly decreased 8 weeks after transplantation MMSCs. Thirdly density of microvasculars was increased at the boundary of infarction site in the animals transplanted MMSCs. Finally,total volume of the myocardial fibrillar collagen was reduced in the MMSCs treated groups after MI. Conclusion Transplanting MMSCs can improve the ventricular remodeling and heart functions in acute MI rat models.
7.Effect of health management in risk behavior control of chronic diseases
Qiuhua WU ; Yumei SHEN ; Guoqin ZHANG ; Li YANG ; Jian LIU
Chinese Journal of General Practitioners 2008;7(9):628-629
Three hundred and ninety-five administrative staffs were randomly selected and provided health management to evaluate its effects in risk factors control of chronic diseases. At 1 year, the rate of people health knowledge and attitude were increased from 14.2% to 39.2%, 7.1% to 25.1%, P≤0.01 ; risk behavior exposure, including incorrect dietary intake, sedentary habit, alcohol consumption or smoking, reduced to some extent (P<0.05).
8.Construction of monitoring system on chemical contaminant in Chinese export plant food and it's application.
Guang-jiang TANG ; Yong-ning WU ; Jian-zhong SHEN
Chinese Journal of Preventive Medicine 2010;44(7):584-586
China
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Food
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Food Contamination
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prevention & control
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Food Inspection
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methods
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Plants
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chemistry
9.Improvement and Application of Drug Procurement Management Module in Our Hospital
Yingkun WU ; Jian ZHANG ; Feng SHEN ; Yan LIU
China Pharmacy 2016;27(22):3099-3101
OBJECTIVE:To improve the drug turnover rate and the work efficiency of drug storehouse,and to reduce the cost of drug storage. METHODS:Based on ABC dynamic classification,original drug procurement management module in HIS was im-proved. Scientific,accurate and reasonable drug procurement plan was generated by the system automatically through setting pro-curement proportion coefficient. Drug procurement by type was achieved through adding classification scanning function,based on distribution mode and drug property classification. Related index were compared before and after improvement to evaluate the ef-fects of the improvement. RESULTS:Compared with before improvement,average monthly inventory amount of drugs in our hos-pital reduced by 6.44%,turnover rate increased by 47.13%(increasing from 4.18% to 6.15%),and the time for developing pro-curement plan decreased by 75.00%(decreasing from 8 h to 2 h). CONCLUSIONS:The improvement of drug procurement man-agement module effectively reduce drug inventory and improve turnover rate of drugs and work efficiency.
10.Establishment and Application of Drug Cold Chain Monitoring Platform in Our Hospital
Feng SHEN ; Jian ZHANG ; Yingkun WU ; Yizong MA ; Rong LIU
China Pharmacy 2017;28(1):91-94
OBJECTIVE:To guarantee the reliability of cold chain drug storage device and the quality of the drugs in the hospi-tal. METHODS:The establishment and effects of drug cold chain monitoring platform in our hospital were introduced as well as re-lated improvement and measures. RESULTS:The cold chain monitoring platform was established in our hospital through equipping with related equipment, constructing platform network, determining monitoring point, setting early warning condition, etc. All-weather and real-time monitoring of temperature and humidity were realized for different districts of the hospital and different cold chain equipments. The platform could provide the information about operating condition of equipment,and complete drug re-frigeration temperature record;equipment failure could be dissolved in time based on early warning message. Through analyzing rea-sons of early warning message,the unqualified equipment was updated in time,which further improve the system and procedure of cold chain monitoring platform. Within 2 years after the implementation of the platform,53 sets of special refrigerator for drug were updated. Monthly early warning message and the rate of refrigeration equipment early warning decreased from 6869 and 100% in Sept. 2014 at the early stage of short message warning function to 1083 and 40% in Feb. 2016,respectively. CONCLU-SIONS:The establishment of drug cold chain monitoring platform guarantees the reliability of cold drug storage condition so as to ensure the quality of drug in the hospital.