2.Etiological analysis of infection after eye surgery and the investigation of drug sensitivity
Jian, WANG ; Pei-tao, YU ; Wei-hua, DANG ; Rui, WEN
Chinese Journal of Experimental Ophthalmology 2013;(5):421-423
Background Clinical researches showed that antimicrobial-resistance of bacterium is gradually serious.It is very important to master pathogen distribution and antimicrobial susceptibility after eye surgery.Objective This study was to investigate the distribution and drug sensitivity of pathogenic bacteria from ocular operative infection in 3-year duration to provide a reference for reasonable selection of antibiotics.Methods Sixtyfive positive specimens were obtained from 65 patients due to postoperative infection in Department of Ophthalmology of Shenzhou Hospital and Shengjing Hospital from 2009 January through 2011 December.The pathogenic bacteria were isolated and then the drug sensitivity test of conventional antibiotics was performed.Results Among the 65 ophthalmic specimens,35 strains of Gram-positive bacteria were isolated with the constituent ratio 53.8%,and 15 strains of Gram-negative bacteria accounted for 23.1%.Fungus was found in 15 cases with the constituent ratio 23.1%.Staphylococcus epidermidis accounted for 42.9% in Gram-positive bacteria,and Pseudomonas aeruginosa accounted for 33.3% in Gram-negative bacteria.Aspergillus was the main component of fungus,which accounted for 60.0% of the proportion 3 strains of methicillin-resistant Staphylococcus aureus (MRSA) in 8 strains of Staphylococcus aureus,but no vancomycin-resistant Enterococcus (VRE) and vancomycin-resistant Staphylococcus (VRS) were seen.The in vitro drug sensitivity test showed that the main Gram-positive bacteria,Staphylococcus epidermidis and Staphylococcus aureus,were sensitive to linezolid,vancomycin,levofloxacin and ciprofloxacin with the sensitive rates >50.0%,and these bacteria were lowly sensitive to penicillin and erythromycin with the sensitive rates ≤30.0%.Pseudomonas aeruginosa appeared to be the main Gram-negative bacteria,showing a sensitive rate ≥ 80.0% to tobramycin,amikacin,ceftazidime,cefepime,cefoperazone/sulbactam,piperacillin/tazobactam,imipenem and meropenem.Conclusions Staphylococcus epidermidis,Staphylococcus aureus,Pseudomonas aeruginosa and Aspergillus are primary pathogens in ocular infection after operation.Antibiotics such as penicillin,erythromycin,levofloxacin,cefotaxime seem to be relatively high resistant to eye infection.To choose sensitive antibiotics timely and reasonably is a key to the prevention and control of ocular infection after operation.
3.Gene mutation and enzymatic activity analysis of α-galactosidase A in a patient with Fabry disease
Wen WANG ; Jian JIANG ; Xiaoli CHEN ; Tao YANG ; Hongwen ZHOU
Chinese Journal of Endocrinology and Metabolism 2010;26(1):27-31
Objective To analyse the clinical characteristics,gene mutation and enzymatic activity of αgalactosidase A(α-GalA)in a 15-year-old male patient with typical Fabry disease,whose mother was without any clinical manifestations.Methods Clinical features and laboratory data were collected from the patient and his mother.Genomie DNA was extracted from peripheral blood of the patient.his mother,and a healthy control subject.Seven exons of the GLA gene were amplified by PCR.PCR products were purified.cloned into T vector,and then sequenced.The enzymatic activity of α-GalA Was measured by fluorimetrie substrate assay. Results DNA sequencing results showed that a missense mutation of 10036-10038delAAG in exon 7 WaS identified in the patient,resulting in the replacement of 374 lysine and 375 glyeine by arginine,which Was not previously reported.The patient Was a hemizygote with gene mutation,his mother WaS a heterozygote carrying gene mutation,and the healthy control without mutation.α-GalA enzymatic activity assay showed that the enzymatic activity of the patient with GLA gene mutation was only 50%of the healthy control subject,while the enzymatic activity of the patient's mother Was about 70%of the heahhy control SObject.Conclusiolls Detecting GLA gene mutation and α-GalA enzymatic activity in patients with Fabry disease who have been clinically diagnosed seelns to be helpful in finding other patients in the family and in further understanding the molecular pathogenesis of that disease.
4.1H-MRS study on the metabolites of first dorsal interossei.
Yi-Hui WU ; Bo YANG ; Tao WANG ; Jian-Zhang JIA ; Min JI ; Chun-Tao YE ; Yi-Wen SHEN
Journal of Forensic Medicine 2014;30(5):329-331
OBJECTIVE:
To estimate the application of prognosis evaluation of ulnar nerve injury by 1H-magnetic resonance spectroscopy (1H-MRS).
METHODS:
The metabolites of first dorsal interossei (FDI) of two hands from 12 healthy volunteers and 1 volunteer with complete ulnar nerve injury were detected by 1H-MRS and the data were statistically analyzed.
RESULTS:
For the FDI of healthy adults, the female peaks area of extra-myocellular lipids (EMCL) was higher than the male (P < 0.05); There was no significant difference in Cho, Cr and intra-myocellular lipids (IMCL) between male and female (P > 0.05); There was no significant difference in all the peaks area between the left and right hand (P > 0.05). The EMCL peak of the injury side was higher than that of the healthy side, and the area of FDI was reduced in the volunteer with ulnar nerve injury.
CONCLUSION
Noninvasive and quantitative detection of 1H-MRS may be valuable for prognosis evaluation of peripheral nerve injury.
Adult
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Female
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Humans
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Male
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Peripheral Nerve Injuries/diagnosis*
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Prognosis
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Proton Magnetic Resonance Spectroscopy/methods*
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Sex Distribution
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Ulnar Nerve/metabolism*
5.Steroids and aromatic derivatives from Euphorbia micractina.
Yao-wu TAO ; Wen-dong XU ; Ye TIAN ; Jian-gong SHI
China Journal of Chinese Materia Medica 2015;40(23):4639-4644
From an ethanol extract of Euphorbia micractina roots, seven steroids fifteen aromatic derivatives were isolated by a combination of various chromatographic techniques, including column chromatography over macroporous resin, silica gel, and Sephadex LH-20 and reversed-phase HPLC. Their structures were elucidated by spectroscopic data analysis as stigamast-5-ene-3beta, 7alpha-diol(1), stigamast-5-ene-3beta,7beta-diol(2), stigmast-5-en-3beta-ol-7-one(3), stigmast-4-en-6beta-ol-3-one(4), stigmast-1, 4-dien-3-one(5), stigmast-3,6-dione(6), beta-sitosterol(7), scopoletin(8), aesculetin(9), 6-hydroxy-5,7-dimethoxycoumarin(10), quercetin(11), 3,3', 4'-tri-O-methylellagic acid(12), p-hydroxyphenylethyl anisate(13), m-hydroxyphenylethyl alcohol(14), (E)-cinnamic acid(15), (E)-ferulic acid(16), 3,4-dihydroxybenzoic acid(17), vanillic acid(18), p-hydroxybenzoic acid(19), ethyl 3,4-dihydroxybenzoate (20), ethyl gallate(21), and methyl gallate(22). These compounds were obtained from this plant for the first time.
Drugs, Chinese Herbal
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chemistry
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Euphorbia
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chemistry
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Molecular Structure
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Spectrometry, Mass, Electrospray Ionization
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Steroids
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chemistry
6.Recent advances in natural product induced DNA damage response in cancer cells.
Guo-wen REN ; Ya-nan NIU ; Jin-jian LU ; Yi-tao WANG ; Xiu-ping CHEN
China Journal of Chinese Materia Medica 2015;40(24):4797-4804
The DNA structures could be altered or even damaged by exogeous or endogenous factors during cell proliferation. Failure of effective and timely repair will lead to cell cycle arrest or apoptosis. By taking the advantage of the quick proliferation of cancer cells, DNA damage induction, cell cycle arrest and apoptosis promotion have become important strategies for ant-cancer chemotherapy. Previous reports showed that an array of natural compounds inhibit cancer cell proliferation by inducing DNA damage, which have therapeutic potentials for anti-cancer drug research and development.
Animals
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Biological Products
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pharmacology
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therapeutic use
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DNA Damage
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Drugs, Chinese Herbal
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therapeutic use
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Humans
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Neoplasms
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drug therapy
7.High carbohydrate and high fat diet induces an increase in carbohydrate response element binding protein in liver of rats.
Jian-hong LIU ; Sen HUANG ; Wen-tao LING
Chinese Journal of Applied Physiology 2009;25(3):294-343
Animals
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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genetics
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metabolism
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Diet, High-Fat
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Dietary Carbohydrates
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administration & dosage
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Dietary Fats
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administration & dosage
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Lipoproteins, IDL
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blood
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Liver
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metabolism
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Male
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RNA, Messenger
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genetics
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metabolism
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Random Allocation
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Rats
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Rats, Sprague-Dawley
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Triglycerides
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blood
8.Observation of curative effect of methylprednisolone with octreotide retrobulbar injection therapy on thyroid associated ophthalmopathy
Xi-Feng, PENG ; Wen-Hao, JIANG ; Jian, YAN ; Jiang-Tao, DENG
International Eye Science 2014;(10):1906-1907
AIM: To explore the curative effect of methylprednisolone with octreotide retrobulbar injection therapy on thyroid associated ophthalmopathy.
METHODS:Fifty- one cases ( 96 eyes ) with thyroid associated ophthalmopathy were treated with methylprednisolone ( 500mg/d ) for 3d, and then retrobulbar injection of octreotide (0. 1mg/mo) for 3mo in one course. Symptoms and signs of the patients were observed before and after the treatment course.
RESULTS: Symptoms of 49 cases were improved significantly. In all cases, the mean value of exophthalmus of the patients was significantly decreased after the treatment by 3. 1 ± 1. 4mm. The height of palpebral fissure was significantly decreased after the treatment by 2. 4 ± 0. 9mm, there were statistically significant difference before and after treatment (P<0. 05).
COCLUSION: Methylprednisolone with octreotide retrobulbar injection therapy can improve symptoms and signs of the patients with thyroid associated ophthalmopathy with high safety and no obvious side effect.
9.Feasibility Research of Determined Oxygen Permeability of Finished Soft Contact Lens.
Yan WEN ; Jian ZHENG ; Honqping FANG ; Xiaohang JIA ; Tao HE ; Weiming QI
Chinese Journal of Medical Instrumentation 2015;39(2):136-138
OBJECTIVEA method for determined oxygen permeability (Dk) of finished soft contact lens.
METHODSOxygen permeability(Dk) of 10 group finished soft contact lens was determined by polarographic method, and the values were linearly calibrated using the measured and established Dk values of the specified reference materials.
RESULTSOnly one Dk value fell outside of the requirement of GB 11417.3-2012 tolerance for Dk (± 20%).
CONCLUSIONIt is great practicability when determined the oxygen permeability (Dk) of finished soft contact lens by polarographic method which were linearly calibrated using the measured and established Dk values of the specified equal thickness reference materials without the measured lens customized 4 different thickness.
Calibration ; Contact Lenses, Hydrophilic ; Oxygen ; metabolism ; Permeability ; Polarography
10.Expression analyses of BcUGT3 and BcUGT6, and their in vitro expression in Escherichia coli.
Yun-Wen TAO ; Jie-Sen XU ; Jing SUN ; Jian-He WEI ; Juan LIU ; Chun SUI
China Journal of Chinese Materia Medica 2014;39(2):185-191
The tissue-specific and MeJA-induced transcriptional levels of BcUGT3 and BcUGT6 in Bupleurum chinense were analyzed in the present study. The transcriptional levels of BcUGT3 in root, leaf, flower and fruit were similar and they all were higher than those in stem. The transcriptional level of BcUGT6 was the highest in leaf and the lowest in flower among in all tested tissues. With non-treated adventitious roots as control, BcUGT6's transcriptional levels were elevated to nearly 2 folds for 2 h, 8 h, 24 h, 2 d and 4 d in MeJA-treated adventitious roots of B. chinense. It showed that the transcriptional level of BcUGT6 was slightly affected by MeJA. While, BcUGT3's transcriptional levels were gradually elevated, and till 4 d after MeJA treatment, the expression level was about 7 folds than that of non-treated control. Using pET-28a (+), the expressions of two genes was investigated. Induced by IPTG, the target proteins were expressed in E. coli and then purified. All the results obtained in the present study will be helpful for follow-up bio-function analysis of BcUGT3 and BcUGT6.
Acetates
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pharmacology
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Bupleurum
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cytology
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enzymology
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genetics
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Cell Membrane
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metabolism
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Cyclopentanes
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pharmacology
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Escherichia coli
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genetics
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Gene Expression
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Gene Expression Regulation, Plant
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drug effects
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Hexosyltransferases
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chemistry
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genetics
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isolation & purification
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metabolism
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Intracellular Space
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metabolism
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Oxylipins
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pharmacology
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Protein Sorting Signals
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Protein Structure, Secondary
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Protein Transport
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Sequence Analysis
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Transcription, Genetic
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drug effects