Anaplasmosis ; complications ; Child, Preschool ; Female ; Humans ; Lyme Disease ; complications

Child ; Guidelines as Topic ; Humans ; Pneumonia, Pneumocystis ; diagnosis ; therapy

Objective To observe the change of gastric mucosal-arterial partial pressure of carbon dioxide gap [p(g-a)(CO2)] in septic shock rabbit.Methods Sixteen anesthetized and mechanically ventilated rabbit were randomly assigned to 2 groups:shock group(n=8) and control group(n=8).The rabbit in shock group were challenged with intravenous injection of 2 mg/kg Lipopolysaccharides from Escherichia coli.The rabbit in control group were intravenous injection of normal saline solution.Mean arterial pressure(MAP) and heart rate were continuously recorded by multichannel physiologic recorder.Cardiac index(CI) and superior mesenteric blood flow index(SMBFI) were continuously monitored by doppler flowmeter.Gastric mucosal partial pressure of carbon dioxide [pg(CO2)] was evaluated by gas tonometry every 10 min.Arterial and venous blood gas analysis,hemoglobin,and lactate levels were measured every 1 hour.Results The parameters remained stable in control group,but the parameters changed significantly in shock group.Compared with baseline levels,2 hours after Lipopolysaccharides infusion in shock group,MAP decreased from(78?5) mmHg to(50?2) mmHg(1 mmHg=0.133 kPa)(F=145.3 P

OBJECTIVETo compare the difference in strength degradation and morphology damage of two dental ceramic materials after Hertzian contact cyclic fatigue.

METHODSHertzian contact technique was used to investigate the response of Empress II glass ceramic and GI- II glass-infiltrated alumina ceramic to cyclic fatigue. Critical loads of specimens after different fatigue cycles were recorded.

RESULTSFor Empress II glass ceramic, critical load had significantly difference between specimens after 10(5) cycles loading. No significant difference of critical load was found in GI- II glass-infiltrated alumina ceramic after cycles loading.

CONCLUSIONGI- II glass-infiltrated alumina ceramic has better capability in resistance to cyclic loading. It may attribute to microstructure of material. Empress II glass ceramic shows a brittle damage model.

Aluminum Oxide ; Aluminum Silicates ; Ceramics ; Dental Porcelain ; Glass ; Humans ; Materials Testing

OBJECTIVEThe aim of the study was to detect the expression of Ki67 and P53 proteins in the gingival tissue before and after wearing three kinds of dental casting alloy crowns.

METHODSThe three kinds of dental casting alloys (58% golden alloy, NiCr alloy, NiCr alloy excluding Be) were used to make full crowns for 3 dogs in each group. Another 3 dogs were taken as control. The immunohistochemistry Envision method was used to measure the expression of Ki67 and P53 proteins in the gingival tissue before wearing the crowns, 2 weeks, 1 month, 2 months, 3 months after wearing the crowns.

RESULTSThe expression of Ki67 and P53 proteins in gingival tissue increased 1 month after wearing NiCr alloy and NiCr alloy excluding Be, then decreased gradually. And the expression of Ki67 and P53 proteins in 58% golden alloy group was similar to that in control. No pathological change was observed in all samples.

CONCLUSIONTwo kinds of NiCr alloy crowns both can increase the expression of Ki67 and P53 proteins in gingival tissue, but 58% golden alloy crowns can not. So compared with NiCr alloy crowns, 58% golden alloy crowns have better biological properties. And none of three kinds of dental alloy crowns may induce long-term abnormal proliferation in gingival tissue.

Alloys ; Animals ; Chromium Alloys ; Crowns ; Dental Alloys ; Dogs ; Gingiva

OBJECTIVETo determine whether the roughness of titanium implant can influence its osseointegration by affecting the growth, alkaline phosphatase (ALP) production and expression of core binding factor alpha 1 subunit (Cbfα1) of osteoblasts.

METHODSTotally 48 titanium disc specimens, 15 mm in diameter and 2 mm in thickness, were included in the study and divided equally into 4 groups with 12 specimens in each group. Specimens were coarsened by sandblasting with carborundum granula and acid etching with mixing liquid of hydrochloric acid and sulphuric acid under diverse conditions. In the four groups, three were treated with different surface roughness: micro-roughness [(1.00 ± 0.20) µm], midrange roughness [(1.67 ± 0.08) µm] and severe roughness [(2.40 ± 0.20) µm], while the group untreated with surface roughness [(0.12 ± 0.03) µm] was set as control. Scanning electron microscope, acridine orange fluorescence staining and coomassie brilliant blue staining were used to observe morphology and growth of osteoblasts incubating on these specimens. Enzyme linked immunosorbent assay (ELISA) and fluorescence quantitative real-time polymerase chain reaction were used to evaluate ALP production and gene expression of Cbfα1 of osteoblasts among different groups.

RESULTSGrowth, ALP production and Cbfα1 mRNA expression of osteoblasts in experimental groups were higher than those in control group (P < 0.05). Significant differences of these data were also found among three experimental groups (P < 0.05). Midrange roughness group showed the highest level of gene expression of Cbfα1 mRNA, which was 0.93 ± 0.03. While that in the micro-roughness group (0.50 ± 0.03) came second, and the severe roughness group had the lowest data, which was 0.37 ± 0.07.

CONCLUSIONSResults indicated that rough surface was more suitable for the adherence and propagation of the osteoblasts than smooth one did. Surface with roughness of 1-2 µm may be a better choice for osseointegration between osteoblasts and dental implants than others are.

Alkaline Phosphatase ; Cell Differentiation ; Core Binding Factor Alpha 1 Subunit ; metabolism ; Dental Implants ; Humans ; Microscopy, Electron, Scanning ; Osseointegration ; Osteoblasts ; metabolism ; Prostheses and Implants ; Surface Properties ; Titanium

OBJECTIVETo observe the effects of Bufei Jianpi Recipe (BJR) on the diaphragmatic neural discharge and the diaphragmatic muscle function in rats with chronic obstructive pulmonary disease (COPD).

METHODSRats were randomly divided into the normal control group, the model group, the high dose BJR group (9.68 g/kg x d(-1)), the medium dose BJR group (4.84 g/kg x d(-1)), the low dose BJR group (2.42 g/kg x d(-1)), and the aminophylline group (2.3 mg/kg x d(-1)). The stable phase COPD rat model was prepared using repeated smoke inhalations and bacterial infections. The high, medium, and low dose BJR and aminophylline was respectively administered to rats from the ninth week to the twentieth week. The sampling was taken. The lung function, diaphragmatic neural discharge time (Td), and diaphragmatic neural discharge interval (Tdi), diaphragmatic neural discharge range (Rd), diaphragmatic neural discharge area (Ad), expiratory time (Tex), inspiratory time (Tin), respiratory rate (RR), respiratory excursion (RE), respiratory area (RA), and diaphragmatic muscular tension and endurance were detected.

RESULTSCompared with the normal control group, the tidal volume (TV), peak expiratory flow (PEF), and 50% tidal volume expiratory flow (EF50) significantly decreased in the model group (P < 0.01). Td, Tdi, Tex, and Tin were significantly prolonged (P < 0. 05, P < 0.01). Ad, Rd, RR, RE, RA, diaphragmatic muscular tension and endurance significantly decreased (P < 0.05, P < 0.01). The ratio of type I and IIA diaphragmatic fibers significantly increased and type IIB significantly decreased (P < 0.01). The activity of ATP decreased and the activity of SDH increased (P < 0.01). The aforesaid indices were improved to different degrees in BJR groups, especially in the high dose BJR group and the medium dose BJR group (P < 0.05, P < 0.01).

CONCLUSIONSBJR could significantly improve the diaphragmatic neural discharge and the diaphragmatic muscle function. Its efficacy was better than that of aminophylline.

Aminophylline ; pharmacology ; Animals ; Diaphragm ; drug effects ; physiopathology ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Male ; Phrenic Nerve ; drug effects ; physiopathology ; Phytotherapy ; Pulmonary Disease, Chronic Obstructive ; drug therapy ; physiopathology ; Rats

From an aqueous extract of Lonicera japonica flower buds, sixteen compounds were isolated by a combination of various chromatographic techniques including column chromatography over macroporous resin, MCI gel, silica gel, and sephadex LH-20 and reversed-phase HPLC. Their structures were elucidated by spectroscopic data analysis as 6'-O-acetylvogeloside (1), 6'-O-acetylsecoxyloganin (2), dichlorogelignate (3), guanosinyl-(3' --> 5')-adenosine monophosphate(GpA,4) , 5'-O-methyladenosine (5), 2'-O-methyladenosine (6), adenosine (7), syringin (8), methyl 4-O-β-D-glucopyranosyl caffeate (9), (-)-dihydrophaseic acid 4'-O-β-D-glucopyranoside (10), ketologanin (11), 7α-morroniside (12), 7β-morroniside (13), kingiside (14), cryptochlorogenic acid methyl ester (15), and 6-hydroxymethyl-3-pyridinol (16). All the compounds were obtained from this plant for the first time, compounds 1 and 2 are new compounds, 3 and 5 are new natural products, and 4 is the first example of dinucleoside monophosphate isolated from a plant extract.
Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Flowers ; chemistry ; Lonicera ; chemistry ; Mass Spectrometry ; Molecular Structure

Objective To confirm the distribution of high arsenic drinking water and the situation of endemic arsenism in Hubei Province, to provide reference basis for prevention and control of endemic arsenic disease. Methods Using typical investigation and sample investigation in 2006 and 2007, the arsenic content of water was detected sampled from 19 counties(cities or communities). And those water samples which were close to or exceeded the stipulated standard were rechecked by the national standard method. Furthermore, the situation of endemic arsenism was investigated in the cities having high arsenic contents of water. Results In 2006,10 028 water samples of 446 villages in 6 counties (cities or communities) were tested, the wells of high arsenic (> 0.05 mg/L) were found in 5 counties (cities or communities) and the proportion of the well that exceeded stipulated standard was 5.29%(530/10 028); In 2007,19 086 water samples of 1282 villages in 17 counties(cities or communities) were tested, the wells of high arsenic were found in 11 counties(cities or communities), and the proportion of the well that exceeded stipulated standard was 1.74%(333/19 086). In these two years, 29 114 water samples were tested, in which 863 water samples were exceeding the stipulated standard. The 2.96% of total wells exceeded stipulated standard and mainly distributed in 179 villages of 12 counties(cities or communities). And the highest arsenic content of water sample was 2.012 mg/L. In the endemic arsenism area, 2 critical, 1 moderate and 1 mild arsenism patients had been found. Conclusions The water of high arsenic content are scattered in Hubei Province and the situation of endemic arsenism disease is mild. Improving water aiming at decreasing arsenic and establishing patient files should be carried out immediately.

Objective To analyze the characteristic of endoscopic submucosal dissection (ESD) in elderly patients aged more than or equal to 80 years. Methods We retrospectively reviewed 41 patients aged more than or equal to 80 years with 47 lesions treated by ESD from September 2010 to January 2017. The diseased region, pathology and complications were evaluated. Results Of the 47 lesions, there have 3 esophageal lesions, 21 gastric lesions, 10 colonic lesions and 13 rectal lesions. For the pathology, there have 34 early cancer or precancerous lesion, 2 GIST, 1 neuroendocrine tumor and 10 other types of pathology. 1 cardia1 early cancer underwent additional laparoscopic lymphadenectomy. There have 7 complications, 4 post-ESD bleeding, 2 delirium and 1 pneumonia. Conclusion ESD for colorectal lesion is more frequently in elderly patients aged more than or equal to 80 years. Post-ESD bleeding is the main complication.