Proton pump inhibitor (PPI) is considered as the standard treatment for acid-related disorders. However, its long-term use, especially in patients with gastroesophageal reflux disease, would cause potential risks, such as hypergastrinemia along with reduced gastric acidity, hyperplasia of enterochromaffin cells (ECL), gastric neoplasms, rebound gastric acid hypersecretion when PPI treatment is stopped, increased oxyntic gastritis in patients with H. pylori infection, and the possible stimulation of growth of non-gastric tumours due to hypergastrinaemia. Each of these trends has led to numerous studies and evaluations on the potential risk-benefit ratio of the long-term use of PPIs, and countermeasures are being proposed for these problems.

Objective: To further study the effects of As 2 O 3 in various concentrations on the esophageal carcinoma cell line. Methods: The esophageal malignant transformed epithelial cells (SHEEC1) were induced by HPV18 in synergy with TPA in our laboratory. The cells cultured in flask and 24 wells plate were treated by As 2 O 3 with concentrations of 1, 3 and 5 ?mol/L for 2, 4, 8, 16, 24 h respectively. The morphologic changes of cells were observed under election microscopy. The mitotic index (MI) of living cells was calculated by phase contrast microscopy and the cells with TdR uptake were examined by autoradiography. The proliferative index (PI) and apoptotic index (AI) were assayed by flow cytometry. Results: A low dosage of As 2 O 3 (1.0 ?mol/L) enhancing the protiferative rate of SHEEC1 was demonstrated with TdR uptake, MI and PI increased. The high AI and low PI were found in the high concentrations (3 and 5 ?mol/L)of As 2 O 3 . The morphological changes of apoptosis and necrosis were found in 24 h after As 2 O 3 in high concentrations (3 and 5 ?mol/L) administrated. Conclusion: The effects of As 2 O 3 in various concentrations are different. Low concentration of As 2 O 3 promotes the proliferation of the esophageal carcinoma cells by increment of DNA synthesis. In high concentration of As 2 O 3 apoptosis and necrosis are induced.

Objective: To investigate the antitumor effects of arsenic trioxide (As 2 O 3 ) on solid tumor in vivo , the transplanted hepatic carcinoma of mice were treated with various dosages of As 2 O 3 . Methods: After 50 mice were injected intraperitoneally with 0,2, 4, 6, 8 ?mol/g As 2 O 3 respectively for observation of acute toxicity, 1, 2, 3 ?mol/g were selected for this experiment. Eighty mice were transplanted with hepatic carcinoma cells (1.5?10 6 cells/each side) into both subaxillae. As 2 O 3 in 0, 1, 2 and 3 ?mol/g were injected into right subaxilla respectively once every day for 10 days. At the 30th day after transplanted hepatic carcinoma cell, mice were killed and the size of solid tumors was measured. The chronic cytotoxicity of As 2 O 3 was observed in these mice.Results: In right subaxillae tumors, which were injected directly with As 2 O 3 , were apparent smaller than that in the left ( P

Objective:To screen differentially expressed proteins in serum proteomic patterns in patients with Rheumatoid Arthritis associated Interstitial Lung Disease by Surface enhanced laser desorption /ionization mass spectrometry with Protein Chip ,and evaluate the value of a preliminary study.Methods: WCX2 ( Weak cation exchange chip ) and Surface enhanced laser desorption/ionization mass spectrometry with ProteinChip were performed to test serum proteins in 19 Rheumatoid Arthritis associated Interstitial Lung Disease,15 Rheumatoid Arthritis patients and 13 health adults,Biomarker Wizard ,Biomarker Pattern and Spss13.0 software were used in combination to analyze the data.Results:The mass/charge ratio ( M/Z) value of 142 protein peaks were detected in serum , there were 4 proteins differentially expressed with statistical significance among them ( P<0.05;Student′s t-test ) , which were the proteins with M/Z 3382.59 ,3453.39 ,11886.0 ,5825.48;the proteins with M/Z 11689.4 ,2266.49 ,1020.22 ,4392.28 ,5074.38 and 2764.69 were selected to establish the optimal diagnostic model.The sensitivity was of was 90%(9/10),the specificity was of 90%(18/20).Conclusion: Surface enhanced laser desorption/ionization time of flight mass spectrum can screen out the significantly different proteins in serum of patients with Rheumatoid Arthritis associated Interstitial Lung Disease.As bomarkers , both of their sensitivity and specificity were high and determination have good prospects for clinical application .

Objective: The present study is to investigate the mechanism of electro-acupuncture in preventing and treating lumbar intervertebral disk displacement. Methods: Seventy-five Sprauge-Dawley rats were used to establish the models of lumbar intervertebral disk displacement by operation, and divided into five groups including electro-acupuncture group, western medicine group, acupuncture plus medicine group, model group and normal control group, then they were treated with corresponding therapeutic method. The expression of inducible nitric oxide synthase (iNOS) mRNA in the lumbar intervertebral disk tissues of rats was detected by RT-PCR. Results: The expression of iNOS mRNA in the lumbar intervertebral disk tissues of rats in model group was compared with that in normal control group, the significant difference was found (P<0.01); While the expressions of iNOS mRNA in the lumbar intervertebral disk tissues of rats in electro-acupuncture group, western drug group and acupuncture plus medicine group were all inhibited, and compared with the model group, the significant differences were also found (P<0.01). Conclusion: Electro-acupuncture could inhibit the expression of iNOS mRNA in the lumbar intervertebral disk tissue.

OBJECTIVE:To guarantee the reliability of cold chain drug storage device and the quality of the drugs in the hospi-tal. METHODS:The establishment and effects of drug cold chain monitoring platform in our hospital were introduced as well as re-lated improvement and measures. RESULTS:The cold chain monitoring platform was established in our hospital through equipping with related equipment, constructing platform network, determining monitoring point, setting early warning condition, etc. All-weather and real-time monitoring of temperature and humidity were realized for different districts of the hospital and different cold chain equipments. The platform could provide the information about operating condition of equipment,and complete drug re-frigeration temperature record;equipment failure could be dissolved in time based on early warning message. Through analyzing rea-sons of early warning message,the unqualified equipment was updated in time,which further improve the system and procedure of cold chain monitoring platform. Within 2 years after the implementation of the platform,53 sets of special refrigerator for drug were updated. Monthly early warning message and the rate of refrigeration equipment early warning decreased from 6869 and 100% in Sept. 2014 at the early stage of short message warning function to 1083 and 40% in Feb. 2016,respectively. CONCLU-SIONS:The establishment of drug cold chain monitoring platform guarantees the reliability of cold drug storage condition so as to ensure the quality of drug in the hospital.

OBJECTIVE:To improve the drug turnover rate and the work efficiency of drug storehouse,and to reduce the cost of drug storage. METHODS:Based on ABC dynamic classification,original drug procurement management module in HIS was im-proved. Scientific,accurate and reasonable drug procurement plan was generated by the system automatically through setting pro-curement proportion coefficient. Drug procurement by type was achieved through adding classification scanning function,based on distribution mode and drug property classification. Related index were compared before and after improvement to evaluate the ef-fects of the improvement. RESULTS:Compared with before improvement,average monthly inventory amount of drugs in our hos-pital reduced by 6.44%,turnover rate increased by 47.13%(increasing from 4.18% to 6.15%),and the time for developing pro-curement plan decreased by 75.00%(decreasing from 8 h to 2 h). CONCLUSIONS:The improvement of drug procurement man-agement module effectively reduce drug inventory and improve turnover rate of drugs and work efficiency.

OBJECTIVE:To assess the ability of suppliers and manufacturers in dealing with drug quality problems so as to provide reference for hospital in optimizing future suppliers and manufacturers.METHODS:The drug quality registration form in drug storehouse in 2007 in our hospital was analyzed in respect of the quality of drugs which were classified into type A and type B.RESULTS:The A-type drug quality problems,i.e drug quality problems presented in the production of drugs, accounted for 28.6%,and B-type drug quality problems,i.e drug quality problems involved in circulation and use of drugs, accounted for 71.4%.CONCLUSIONS:Drug quality data analysis report can objectively reflect the overall condition of hospital drug quality and the drug quality of the suppliers,thus it can be used as reference for hospital in optimizing future drug manufacturing enterprises and suppliers.

Three hundred and ninety-five administrative staffs were randomly selected and provided health management to evaluate its effects in risk factors control of chronic diseases. At 1 year, the rate of people health knowledge and attitude were increased from 14.2% to 39.2%, 7.1% to 25.1%, P≤0.01 ; risk behavior exposure, including incorrect dietary intake, sedentary habit, alcohol consumption or smoking, reduced to some extent (P<0.05).

Objective To investigate the role of interleukin-27 (IL-27) in proliferation and differentiation of CD4+ T cells in ankylosing spondylitis(AS).Methods CD4+ T cells were separated from peripheral blood which collected from AS patients and health controls(HCs).Cells proliferation was detected by CCK-8 kit,and cytokines were analyzed by ELISA.Real-time PCR was used to determine the mRNA expression of T-bet and GATA3.Results Serum IL-27 level in patients with AS was obviously higher than that in HCs(P ＜0.01).The proliferation rate of CD4+ T cells and the level of IFN-γin cultured medium in AS were higher than those in HCs group after IL-27 stimulation (P ＜ 0.01).IL-27 could induce T-bet mRNA expression in CD4+ T cells in AS (t =14.3,P ＜ 0.01),but no change was found in GATA3 mRNA expression.Conclusions IL-27 could induce the proliferation of CD4+ T cells and the activation of T-bet pathway in AS.Furthermore,Th1 immune response and related cytokines could be induced by IL-27 in AS.These implicate that IL-27 may play an important role in AS related inflammation.