1.Experimental study of the protective effect of combined medication on acute cerebral ischemic reperfusion injury
Chun-hua PIAO ; Shu-rong JI ; Jian-peng XU
Chinese Journal of Rehabilitation Theory and Practice 2002;8(9):529-530
ObjectiveTo study neural protective effect of combined medication with nimodipine and mannitol on injury of cerebral ischemia-reperfusion for screening the better medication method in acute cerebral ischemia-reperfusion. MethodsA model of cerebral ischemia-reperfusion was performed by clipping bilateral common carotid artery of rats with vago- and releasing them 3 hours later. 40 Wistar female rats within 1 month were divided into 5 groups randomly with 8 rats each: model group (no use of medicine), nimodipine group(0.2mg/kg), mannitol group(0.5g/kg), nimodipine and mannitol group, sham-operated group (no use of medicine and no clipping process). The changes of SOD and MDA in brain tissue were measured 24 hours after cerebral ischmic reperfusion in all groups. At the same time pathologic study was performed to compare the different groups.ResultsThere were significant differences between nimodipine and mannitol group and other groups in changes of SOD, MDA and pathological changes(P<0.01). Conclusions Combined medication with nimodipine and mannitol is the better way to protect brain tissue from acute cerebral ischemia-reperfusion than other way in present experiment, by synergistic action.
2.Experimental observation of electrical stimulation on cultured nervous system cells
Zheng-mei WANG ; Jian YANG ; Rong-hua LU ; Yongqi XU
Chinese Journal of Rehabilitation Theory and Practice 2002;8(11):664-665
ObjectiveTo explore the influence of electrical stimulation on nervous cells.MethodsOn 5th day the brain primitive nervous cells of rat brain, in vitro culturing, were divided into 4 groups, in order of control group, electrical pole control group, low stimulation group, and high stimulation group. The cells were stimulated with electro-acupuncture apparatus for 7 days, 30 minutes once, twice a day. On 10th day, the cells were observed microscopically for 3 days and taken photos.ResultsElectrical stimulation is helpful to raise the survival rate of cell.Conclusions Electrical stimulation influences morphological change of nervous cells during culturing.
3.Clinical value of the ECT bone scan in diagnosis of prosthesis infection for revision.
Hong-Yao XU ; Jian-Ning ZHA ; Ni-Rong BAO
China Journal of Orthopaedics and Traumatology 2015;28(3):219-221
OBJECTIVETo detect the clinical value of the ECT bone scan in evaluating of the situation of infection control after hip knee arthroplasty.
METHODSThe clinical data were retrospectively analyzed in 62 patients, including 34 males and 28 females with an average age of 68.8 years old ranging from 65 to 74 years. The results of ECT bone scan, erythrocyte sedimentation rate, C-reactive protein were used to assess periprosthetic infection. The patients with positive ECT and ESR on CRP were considered to have periprosthetic infection; however, the patients with two or more negative, indexes were considered to have no infection.
RESULTSThe sensitivity, specificity, accurate rate of ECT were 75.0%, 88.9%, 87.1% respectively; ESR 50.0%, 72.2%, 69.4%; CRP 62.5%, 81.4%, 79.0%. The combination of the three methods were 87.5%, 96.3% and 95.2%,
CONCLUSIONCompared with ESR and CRP, ECT is a more effective way in the diagnosis of periprosthetic infection, which has great value and is worth popularizing.
Aged ; Arthroplasty, Replacement ; adverse effects ; Blood Sedimentation ; Bone and Bones ; diagnostic imaging ; C-Reactive Protein ; analysis ; Female ; Humans ; Male ; Prosthesis-Related Infections ; diagnostic imaging ; Retrospective Studies ; Tomography, Emission-Computed ; methods
4.Effect of lipid emulsion on cardiac arrest induced by Bupivacain intoxication
Jieyu FANG ; Nan JIANG ; Kangqing XU ; Jian RONG ; Tao ZHANG
Chinese Journal of Primary Medicine and Pharmacy 2009;16(z2):6-8
Objective To investigate effect of lipid emulsion on cardiac arrest induced by bupivacain intoxication.Methods Forty SD rats were randomly devided into 2 groups.Group A applied epinephrine (n=20);Group L applied lipid emulsion combinding epinephring(n=20).The rats were administered Lupivacaine 20 mg/kg.Cardiopulmonary resuscitation(CPR) was started. Epinephrine 10 μg/kg were repeated three times followed by epinephrine 10 μg/kg every 5 minutes.Normal saline 5 ml/kg and 1 ml·kg-1·min-1 were administered in group A.20% Lipid emulsion 5 ml/kg and 0.25 ml·kg-1·min-1 were administered in group L.Heart rate,blood pressure, temperature were monitored.Blood gas analysiswere checked at 5 min,30 min after restoration of spontaneous circulation(ROSC).To record the number of successful CPR,time of ROSC and epinephrine dose.Results 11 rats(55%) and 16 rats(80%) were successful resuscitated.The success rate was higher in group L than in group A.Epinephrine dose was higher in group A than in group L.There was no difference in heart rate between the two groups.Systolic blood pressure and PaO2 was higher in group L than in group A(P<0.05).Conclusion There was better effect in lipid emulsion combined epinephrine than soly epinephrine when applied in cardiac arrest induced by bupivacaine intoxication.
5.Effect of Bacillus Calmette-Guerin Intervention on Activation and Apoptosis of T Lymphocyte in Asthmatic Mouse
rong-ling, GE ; jian-hua, ZHANG ; lun, XU
Journal of Applied Clinical Pediatrics 1994;0(04):-
Objective To explore the effect of bacillus calmette-guerin (BCG)intervention on expression of costimulatory molecules of B7-CD28/CD152 and T lymphocyte apoptosis in asthmatic mouse.Methods Twenty-seven mice were randomly divided into:asthma model group,BCG treatment group and normal control group.The mice were sensitized by ovalbumin(OVA) and 100 g/L Al(OH)3 with intraperitoneal injection,challenged with atomization inhalation,then reproduced the asthmatic models.The BCG treatment group were treated with BCG(0.025 mg,merely) subcutaneous injection 3 times before sensitization(5,3,1 day).The normal control group were treated with 9 g/L saline water taking place of OVA and Al(OH)3.CD28,CD152,CD80 and CD86 were analyzed by flow cytometry in splenic lymphocyte suspension.T lymphocyte was cultivated from peripheral blood mononuclear cell.The apoptosis ratio of T lymphocyte was counted by Comet Assay.SPSS 10.0 software was applied to analyze data.Results Contrasting asthma model group with normal control group,CD28 was up regulated(t=3.94 P0.05),CD86 was up regulated(t=2.68 P0.05).Contrasting BCG treatment group with asthma model group,T lymphocyte apoptosis rate was higher(t=3.15 P
6.Observation on Effect of Huatan Quyu Tablets in Treating Carotid Atherosclerosis
Jian WANG ; Rong YU ; Jieting WANG ; Xiangguang XU
Journal of Guangzhou University of Traditional Chinese Medicine 2004;0(06):-
[ Objective ] To investigate the effect of Huatan Quyu Tablets in treating carotid atherosclerosis ( CA). [Methods] Color Doppler ultrasonographic technique was used to detect the mid-intima thickness (MIT) and carotid resistance (CR) in 40 cases of CA before and after treatment with Huatan Quyu Tablets . [Results] MIT and CR were both decreased after treatment (P
7.Effects of early rehabilitation on upper extremity function of patients with shoulder-hand syndrome after stroke
Shao-ling WU ; Li-rong HUANG ; Jian-xing XU
Chinese Journal of Rehabilitation Theory and Practice 2002;8(1):24-25
ObjectiveTo study the effects of early rehabilitation on symptom, range of motion (ROM) and motor function of upper extremity of patients with shoulder-hand syndrome after stroke.Methods47 patients were divided into two groups,early rehabilitation group and non-early rehabilitation group.Two groups were treated with comprehensive rehabilitation. The passive ROM, the pain and motor function of affected limb was assessed by Fugl-Meyer Assessment.The evalution was done in pre-treatment and post-treatment respectively. Results1.The passive ROM decreased and the pain of affected limb of the early rehabilitation group were less than the non-early rehabilitation gronp when the shoulder-hand syndrome occour.2.After comprehensive rehabilitation treatment,their clinical symptom ROM and motor function of upper extremity impoved significantly in patients with shoulder-hand syndrome after stroke( P<0.05).The early rehabilitation treatment has better effects. Conclusions The early rehabilitation treatment could decrease the degree of their passive ROM decrease and the pain of affected limb in patients with shoulder-hand syndrome after stroke,and the patients who accepted earlyrehabilitation seemed easly to improve by comprehensive treatment.
8.Overview of management system for conflict of interest of the European Pharmacopoeia Commission and the United States Pharmacopoeia Convention
XU Xinyi ; LIU Jian ; ZHANG Lin ; SHEN Xueyao ; ZHAO Jianfeng ; ZHANG Jun ; FU Jian ; SHU Rong
Drug Standards of China 2024;25(1):0103-0108
This article introduces an overview of management system for conflict of interest of the European Pharmacopoeia Commission (EPC) and the United States Pharmacopoeia Convention (USP). The EPC and USP have standardized the management system for conflict of interest in drug standard work in multiple management documents, such as the Guide for the Work, Code of Practice for the Work, Form for Declaration of Interests and Confidentiality Undertaking of the EPC, bylaws, Rules and Procedures of the Council of Experts, Code of Ethics, Standards of Conduct of the USP, in order to ensure the transparency and fairness of drug standard development, improve the credibility and rigor of drug standards. This article introduces the management system for conflict of interest of the EPC and USP, providing reference for the improvement of relevant management systems of the Chinese Pharmacopoeia Commission.
9.Investigation on a outbreak of hepatitis A.
Chinese Journal of Epidemiology 2006;27(12):1100-1100
10.Drug reservation of community residents in Shanghai
Zhen LI ; Zongjun FANG ; Mingjian GE ; Jian XU ; Rong YANG ; Mei YANG ; Hanyan SHI
Chinese Journal of General Practitioners 2008;7(12):837-838
Drug reservation wag investigated in 2077 community residents.We found that most drugs were obtained from the hospitals(83.78%),kept at a relatively lower place(69.23%),packed in box(75.25%),and did not meet the storage requirement(72.60%).Half of the overdue drugs(median time,12 months)were used for internal treatment.This study suggests that there might be unsafe drug storage in communities.