Objective: To observe the morphological changes and analyze the differential protein spectrum of human malignant glioma cells SHG-44 after treated with Nordy (Chinese patent number: ZL02133700.4), an analog of Nordihydroguaiaretic acid. Methods: The differentiation of SHG-44 cells was induced by 100 μmol/L or 200 μmol/L Nordy; the morphological changes of cells were observed 24, 48 and 72 h after Nordy treatment and the findings were compared with those of the control group (received no treatment). The total proteins were extracted from SHG-44 cells treated with 200 μmol/L Nordy for 72 h and cells in control group, then were subjected to two-dimensional gel electrophoresis. PDquest 7.1 software was employed to compare the protein expression differences. The highly expressed differential proteins were identified by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Results: The morphological changes of SHG-44 cells treated with 200 μmol/L Nordy were more obvious than those treated with 100 μmol/L Nordy, and the most obvious differentiation was found in the cells treated for 72 h. Compared with those of control group, 23 differential protein spots were identified by the two-dimensional electrophoresis, including 21 down-regulated ones and 2 up-regulated ones. MALDI-TOF-MS showed that the highly expressed proteins were: an unknown protein, proliferation-associated gene A, Up1, alternative splicing factor ASF-3, cofilin1(non-muscle), eukaryotic translation initiation factor 5A, beta galactoside binding lectin, and glutathione-S-transferase Pi. Conclusion: Nordy can induce differentiation of human malignant glioma cells SHG-44 in a time-effect and dose-effect dependent manner. The Nordy-induced differential proteins may function in multiple aspects such as cell proliferation, apoptosis and gene transcription.

With the development of computer techniques and medical imaging examining methods , precise radiotherapy is becoming the major direction of radiotherapy for tuomors. Both of tumor control probability and normal tissue complication probability are improved with precise radiotherapy. This paper critically review the value of PET-CT and breathing control in precise radiotherapy for non-small-cell lung cancer (NSCLC).

Objective To study the combined effect of chlorotoluron and atrazine on the testis of mice. Methods Kunming mice were divided into groups and treated with chlorotoluron and atrazine alone or in combination by gavage at different doses for 25 consecutive days. Microscope and electron microscope were used to observe the morphological changes. Results The herbicides used alone or in combination, at all the test doses, caused the morphological changes in degrees in the testis. Under the light microscope, seminiferous epithelium arrayed loosely and disorderedly, spermatogenic cell shed, and layers lessened compared with the control group. Under the electron microscope, mitochondria in the seminiferous epithelium appeared vacuolated, karyotheca swelled, bent and the function of sustentacular cell declined. Compared with alone use groups, the pathological changes were more serious in combination use groups. Conclusion Chlorotoluron and atrazine can produce a combined toxic effect on the testis of mice.

This paper aimed to introduce and review the antioxidant and material base of selaginella medicinal plants.On the basis of the systematic identification of selaginella medicinal plants,we found that the ethyl acetate extract and flavonoids could inhibit the activity of XOD,LOX or/and COX-2 to some extent.Fourteen biflavonoids,one flavonoid and five flavonoid glycosides have been isolated from three resource plants,among them five compounds were firstly reported and fifteen compounds were firstly isolated in selaginella plants.Amentoflavone (AME),robustaflavone and robustaflavone-4''-dimethylether are three common characteristic constituents of selaginella plants,which can act as marks of common active ingredients and be applied to evaluate the quality of selaginella.Further studies found that some plants had stronger anti-Coxsackie virus and anti-tumor effects,and their mechanisms were associated with inhibiting multiple oxidase and mitigating harm of free radicals due to whole function.

Autophagy ; Liver Cirrhosis ; physiopathology

Objective To study the relation between the effect of Curcumin on the proliferation and activation of hepatic stellate cells(HSC)and the expression of the peroxisome proliferator-activated receptor?(PPAR?).Methods The rat HSC was isolated from SD rats through in situ perfusion of liver with Pronase E and density-gradient centrifugation with Nycodenz.The subcultured cells were treated with corresponding compound.The inhibition effect on HSC proliferation was determined by MTT colorimetry.The total RNA was extracted by TRizol reagent,and the gene expression level of PPAR? and smooth muscle actin (?-SMA)were determined by semi-quantitative RT-PCR.The total cellular proteins were extracted and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis,and the protein level of ?-SMA was determined by Western blotting.Results The MTT analysis results showed that Curcumin inhibited HSC proliferation between 10 and 50 ?mol/L in a dose-dependent manner.In the Day 1,4,7 of primary HSC and passaged HSC,the expression of PPAR? mRNA decreased with HSC activation extent;Curcumin could up-regulate the expression level of PPAR?.Curcumin suppressed the expression of ?-SMA not only at the gene level but also at the translation level.But all these effects of Curcumin on HSC could be blocked by the PPAR? specific antagonist GW9662.Conclusion The effect of Curcumin on HSC cell proliferation and activation is through the up-regulation of PPAR? signal.

Aim To study the effect of Curcumin on the apoptosis of hepatic stellate cells (HSC), and the correlation between the effect and peroxisome proliferator-activated receptor ?(PPAR?) signal.Methods The HSC was isolated from normal SD rats through in situ perfusion of liver with protease E and density-gradient centrifugation with Nycodenz.The subcultured cells were treated with corresponding compounds. Cell apoptosis was detected by Hoechst 33258 staining. PPAR? subcellular distribution was detected by immunofluorescent staining. Total RNA, total protein and nuclear protein were extracted respectively, target gene and protein levels were determined by semi-quantitative RT-PCR or Western blot.Results There was nearly no apoptosis in activated HSC. Curcumin treatment induced the apoptosis of HSC, enhancing PPAR? nuclear translocation/redistribution.At the transcription and translation level,curcumin upregulated nuclear PPAR? expression, inhibited anti-apoptotic Bcl-2 expression, and promoted pro-apoptotic Bax expression; but all these effects could be reversed by PPAR? antagonist GW9662.Conclusions Curcumin induces HSC apoptosis by enhancing PPAR? expression and nuclear translocation/redistribution.

To explore the effect of tripterygium glycosides on the level of peripheral blood cell factors of Graves ophthalmopathy (GO). In the study, 64 patients of GO in moderate-severe acute stage were selected, and randomly divided into the treatment group (32 cases) and the control group (32 cases). Both of the two groups were provided with basic treatment. The control group was added with prednisone(0. 75 mg kg-1 d-1 ), which gradually reduced (by 5-10 mg week-1 )to the minimum dose of 5 mg d-1. The treatment group was treated with 20 mg tripterygium glycosides, three times a day. One therapy course is three months. The levels of peripheral blood cells(TNF-alpha , IL-2, IL-10, IFN-gamma)of the two groups before and after the treatment and the clinical efficacy were observed. The study indicated that, before the treatment, TNF-alpha, IL-2, IFN-gamma in both groups were significantly higher than that in the health group, but with IL-10 notably lower than the healthy group. After the treatment, TNF-a, IL-2, IFN-gamma in the treatment group significantly decreased, but with IL-10 significantly increasing (P <0. 01). After the treatment, the two groups showed significant difference (P <0. 01). The total clinical efficacy in the treatment group was 88. 10% , and that in the control group was 57. 14% (P <0. 01). After the treatment, the two groups showed significant changes in the exophthalmos degree (P < 0. 01). The results showed that the level of peripheral blood cells (TNF-alpha,IL-2, IL-10, IFN-gamma)of GO patients was positively correlated with the severity of ocular disease. The combined therapy of tripterygium glycosides and methimazole show such advantages as low side effect and high clin-
Adult ; Cytokines ; blood ; Female ; Glycosides ; pharmacology ; therapeutic use ; Graves Ophthalmopathy ; blood ; drug therapy ; Humans ; Male ; Tripterygium ; chemistry

Objective To explore the effect of circadian rhythm disorder on cognitive decline and neurologic impairment after traumatic brain injury (TBI) in rats.Methods Rats were subjected to a weight-drop model of TBI,then the rat models were divided into 2 groups ac-cording to the environmental length of day and night ,namely,the whole day group (12 h light/12 h light) and the control group (12 h light/12 h dark).After 14 days,the Morris water maze test and step-down test were carried out to evaluate the memory of the rats in each group . HE staining and immunohistochemistry ( BrdU, Ki-67) were carried out to evaluate the degree of the neurologic impairment of the rats in each group.And the mRNA expressions of Caspase-3,Caspase-9,Bcl-2 and Bax were evaluated with realtime PCR .Results The memory function of the rats in whole day group was significant lower than control group ;the damage degree of the cells in the hippocampus and the cortical lesion volume in whole day group were significant higher than control group ;the cell proliferation rate and newborn cell survival rate in the whole day group decreased significantly compared with the control group ;the mRNA expression of Bcl-2 in whole day group was signifi-ant lower than control group ,and the mRNA expression of Caspase-3,Caspase-9 and Bax in whole day group was signifiant higher than control group.Conclusion Circadian rhythm disorder can worsen TBI-induced cognitive decline and neurologic impairment .

0.01). The tumor volume and the tumor weight were also significantly decreased in the treated group (P