1.A STUDY ON PREPARATION AND QUALITY STANDARD OF XUENING GRANULE
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(03):-
Xuening Granule is a traditional Chinese patent medicine based on San Huang Xie Xin Tang. The effective Component of each herbal medicine were extracted individually and then mixed together to form granules. This process avoids the chemical reactions among the drugs in order to enhance the the effects. The major contents are determined and differentiated to control its quality
2.The biotin synthesis pathway in Mycobacteria tuberculosis is a new target for the development of anti-tuberculosis drugs
Acta Pharmaceutica Sinica 2024;58(3):503-510
italic>Mycobacterium tuberculosis, responsible for tuberculosis (TB), remains a major health problem worldwide and is one of the infectious diseases causing increased morbidity and mortality worldwide. Biotin, namely vitamin H, is an important cofactor necessary for fatty acid biosynthesis, gluconeogenesis and amino acid metabolism in organisms including
3.Framework analysis method in qualitative research.
Xing LIAO ; Jian-ping LIU ; Nicola ROBISON ; Ya-ming XIE
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(5):622-626
In recent years a number of qualitative research methods have gained popularity within the health care arena. Despite this popularity, different qualitative analysis methods pose many challenges to most researchers. The present paper responds to the needs expressed by recent Chinese medicine researches. The present paper is mainly focused on the concepts, nature, application of framework analysis, especially on how to use it, in such a way to assist the newcomer of Chinese medicine researchers to engage with the methodology.
Humans
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Medicine, Chinese Traditional
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Qualitative Research
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Research
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Research Design
4.The diagnosis and treatment of acute pancreatitis during pregnancy:a report of 48 cases
Jian PI ; Ping ZHOU ; Xiaohong XIE ; Xiong HUANG
Chinese Journal of General Surgery 1993;0(03):-
Objective To study the clinical characters,diagnosis and principles of treatmentof acute pancreatitis during pregnancy.Methods Forty-eight cases of acute pancreatitis during pregnancy treated in the recent 8 years were retrospectively analyzed,38 cases had non-surgical treatment(of which 36 cases were cured);10 cases had surgical treatment to remove necrotic tissue and mainly termination of pregnancy.Results Most of the cases in this group had simple pancreatitis,which accounted for 64.58%(31/48).There were 5 cases of maternal(10.42%) death and three cases of fetal(6.25%) death,and all were in patients with severe pancreatitis.Fatality rate of pregnant women with severe pancreatitis was 29.41%.Conclusions Acute pancreatitis during pregnancy is a serious disease with low prevalence rate,but has high fatality rate,and is the primary cause of death in acute abdomen of pregnancy.Nonoperative treatment is the first choice of treatment in this condition.In severe cases,timely surgical intervention is warranted,and the surgical indications should not be altered because of the pregnancy.The safety of both the pregnant woman and the fetus must simultaneously be kept into mind during therapy.
5.Study on Extraction Process of Volatile Oil and Inclusion Preparation of Hydroxypropyl-β- cyclodextrin from CompoundJinling Sini Siwu Shixiaosan Granules
Chunxia BAI ; Ping XIE ; Jian FENG ; Chuan JIANG ; Haiyan NING
Chinese Journal of Information on Traditional Chinese Medicine 2015;(9):95-97,98
Objective To optimize the extraction process of volatile oil and inclusion preparation of hydroxypropyl-β-cyclodextrin from compoundJinling Sini Siwu Shixiaosan Granules.Methods The orthogonal approach of L9 (34) was applied to investigate the volume of volatile oil extraction by 3 factors to obtain the optimum extraction conditions. With inclusion rate of volatile oil and yield of inclusion compound as comprehensive evaluation indexes, the optimum preparation conditions were discussed. The characterization of this prepared inclusion was analyzed by TLC.Results The optimum extraction conditions were as follows:the crude medicine with medicinal powder was shattered into 20 meshes;8 times the amount of water was added and was extracted for 8 h. The best inclusion conditions were as follows:the proportion of volatile oil to HP-β-CD was 1∶6, with inclusion temperature of 30℃, grinding time of 2 h. Analysis results of TLC showed that stable inclusion compound was formed from volatile oil and HP-β-CD.Conclusion The optimized extraction process and inclusion preparation are with stable quality and high feasibility.
6.Construction of rice stripe virus NS2 and NS3 Co-RNAi transgenic rice and disease-resistance analysis.
Lu-ping ZHENG ; Chen LIN ; Li-yan XIE ; Zu-jian WU ; Lian-hui XIE
Chinese Journal of Virology 2014;30(6):661-667
NS2 and NS3 are two post-transcriptional gene silencing suppressors that are encoded by Rice stripe virus. Gene silencing suppressors are always related to the pathogenicity of viruses. In this study, the cDNA of NS2 and NS3 were recombined by overlapping PCR assays, ligated to the RNAi vector, and inserted into the PXQ expression vector using Pst I; the expressed vector was transferred into calluses induced from seeds of the japonica rice cultivar, 'Nipponbare', using an Agrobacterium-mediated method. Thirty-one T0 transgenic plants were selected by G418 screening. PCR and southern blot analyses confirmed that the target gene was transformed into transgenic rice successfully, and different transgenic plants contained various copies of the gene. The disease resistance assay revealed that T0 transgenic rice had a delayed onset of RSV for approximately 10-20 d, and the accumulation of virus in the transgenic plants was reduced by 30%-50%. This was related to the delayed onset of disease.
Disease Resistance
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Oryza
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genetics
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immunology
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virology
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Plant Diseases
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genetics
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immunology
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virology
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Plants, Genetically Modified
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genetics
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immunology
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virology
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RNA Interference
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Tenuivirus
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genetics
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immunology
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Viral Nonstructural Proteins
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genetics
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immunology
7.Fingerprint of X-ray diffraction of Tibetan medicine dairy Nanhanshuishi and its application in processing by microwave.
Lin-yan ZHU ; Jian-feng XIE ; Zi-ming KONG ; Gao-ping LI ; Yong-ping HAN
China Journal of Chinese Materia Medica 2015;40(10):1948-1952
The characteristic fingerprint of conventional dairy Nanhanshuishi was established by X-ray diffraction (XRD), based on similarity of caculation on public peaks by MATLAB software, and the feasibility of new dairy technology of microwave method was explored between XRD and the dissolution rate in artificial simulation gastric juices. The result showed that similarity of shared peak in XRD of conventional dairy Nanhanshuishi was > 95%, This XRD characteristic fingerprint of conventional dairy Nanhanshuishi had strong specificity, could be used to provide a reference for identification and quality evaluation. This study also showed that the similarity of microware dairy products and conventional dairy products was good, and the sample of microwave 15 min was the best, and new dairy method by the microwave could replace the traditional method.
Animals
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Chemistry, Pharmaceutical
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instrumentation
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methods
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Drugs, Chinese Herbal
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chemistry
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Medicine, Tibetan Traditional
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Microwaves
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Milk
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chemistry
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Plants, Medicinal
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chemistry
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Quality Control
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X-Ray Diffraction
8.RNA-Seq and genuine traditional Chinese medicine.
Xiao-Yue WANG ; Jing-Yuan SONG ; Cai-Xiang XIE ; Jian-Ping HAN ; Shi-Lin CHEN
Acta Pharmaceutica Sinica 2014;49(12):1650-1657
RNA-Sequencing (RNA-Seq) is a newly-developed method in transcriptome research, it can afford more accurate transcription information and be more quickly by using Next-generation Sequencing (NGS) technology. RNA-Seq has been widely used in various biological fields. Genuine traditional Chinese medicines (TCM), with good quality and therapeutic effect, were always praised highly and used by famous physicians. The geo-herbalism formation of TCM is based on the product of the gene expression at specific space and time. So it has been a research hotspot to analyze the mechanism of biosynthesis through RNA-Seq in the study on the secondary metabolism of medicinal plant. This article mainly illustrates the RNA-Seq and its advantages, it also discusses the potential application in genuine TCM, and it can provide useful information for other researchers.
Drugs, Chinese Herbal
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Gene Expression Profiling
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High-Throughput Nucleotide Sequencing
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Medicine, Chinese Traditional
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Plants, Medicinal
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genetics
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RNA
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Sequence Analysis, RNA
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Transcriptome
9.Correlation analysis on combined medication with of Xiyanping injection in treatment of lung infection in real world.
Xiu-ping YIN ; Yan-ming XIE ; Ying-jie ZHI ; Wei YANG ; Zhi-fei WANG ; Jian HUO
China Journal of Chinese Materia Medica 2015;40(12):2440-2444
To analyze the regularity in combined medication with Xiyanping injection (Xiyanping for short) in the real world by as- sociation rules. Totally 5 822 patients using Xiyanping injection was collected from the 18 Class III Grade I hospitals nationwide to study the combined medication information of the patient with lung infection and make the analysis by using association rules and Apriori. According to the results, major drugs combined with Xiyanping in treatment of lung infection included compound amino acid, inosine, coenzyme A, cytidine triphosphate, vitamin C. Common drugs combined with Xiyanping can be divided into 5 categories: nutrition support therapy (vitamin C, compound amino acid) , coenzymes (coenzyme A, cytidine triphosphate, inosine), expectorants and antiasthmatics (ambroxol, salbutamol, doxofylline), hormones (dexamethasone, budesonide), antibiotics (mainly cefminox). The main combined medicines mostly conformed to the regularity for drugs treating lung infection. In addition, there were two most common medical combination models: the model for Xiyanping combined a single medicine is Xiyanping + nutrition support therapy, while the model for Xiyanping combined two or more than two medicines is Xiyanping + nutrition support therapy + coenzyme. Pharmacologically, Xiyanping is mostly combined with western medicines with similar pharmacological effects to substitute or supplement the antibiotic effect in treating lung infection. However, further studies shall be conducted for the safety and rationality of the combined medication based on clinical practices, in order to provide reference for clinical medication.
Adult
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Anti-Bacterial Agents
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administration & dosage
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Ascorbic Acid
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administration & dosage
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Cephamycins
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administration & dosage
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Drug Therapy, Combination
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Drugs, Chinese Herbal
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administration & dosage
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Female
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Hospital Information Systems
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Humans
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Lung Diseases
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drug therapy
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Male
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Middle Aged
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Young Adult
10.Changes of body weight, blood glucose in chronic intermittent hypoxic rats and protection of iptakalim.
Hong SHEN ; Wei-ping XIE ; Hong WANG ; Ya-qin ZHAI ; Jian-kang CAI
Chinese Journal of Applied Physiology 2010;26(2):215-248
Animals
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Blood Glucose
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drug effects
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Body Weight
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drug effects
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Chronic Disease
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Female
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Hypoxia
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physiopathology
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KATP Channels
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drug effects
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Male
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Propylamines
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pharmacology
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Protective Agents
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pharmacology
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Rats
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Rats, Sprague-Dawley