Ascorbic Acid ; adverse effects ; immunology ; Child, Preschool ; Drug Hypersensitivity ; etiology ; Family ; Female ; Humans ; Male

Aluminum ; urine ; Humans ; Metallurgy ; Occupational Exposure ; analysis ; Reproducibility of Results ; Spectrophotometry ; methods

Angioplasty, Balloon, Coronary ; Humans ; Myocardial Infarction ; therapy

Objective: To investigate the effect of ginseng polysaccharide on the gene expression profile of human erythroleukemia K562 cells cultured in vitro. Methods: The total RNAs treated with ginseng polysaccharide for 48 h were extracted from both K562 cells and the control K562 cells. All the RNAs were purified into mRNA and then convened to double-stranded cDNAs by reverse transcription. The cDNAs were labeled with a fluorescence dye Cy3 or Cy5 and hybridized to a Illumina whole-genome oligonucleotide microarray. The change in the gene expression profile of K562 cells following treatment with ginseng polysaccharide was analyzed by using bioinformatics. Results: A total of 306 differential genes were found. Of which 220 were up-regulated and 86 were down-regulated. Their biologic function was classified. Conclusion: Tumor cell differentiation is multiple genes comprehensive results, the screening of the genes may be meaningful for understanding tumorigenesis, tumor development, reverse polarization, and finding potential drug targets.

Objective To investigate the correlation of spiral CT(SCT)features with pathology, microvessel density(MVD)、expression of vascular endothelial growth factor(VEGF)、matrix metalloproteinase-2(MMP-2)in colorectal carcinoma.Methods Forty patients with colorectal carcinoma confirmed by operation were examined by SCT.The resected tumor specimens were immunohistochemically stained for the expression of VEGF,MMP-2 and the calculation of MVD.Results The accuracy of SCT in depicting the pericolic and wall infiltration was 92.5%.The metastasis rates of colorectal cancer with pericolic infiltration and wall infiltration were 75.0% and 33.3%,respectively,the differences were statistically significant between the two groups(P

Objective To explore the relation between endothelial function and Alzheimer′s disease(AD).Methods The concentration of asymmetric dimethylarginine(ADMA)and L-arginine were measured by high-performance liquid chromatography(HPLC),the concentration of nitric oxide(NO) detected by the method of nitrate reductase,and the activity of nitric oxide synthase(NOS)examined using NOS assay kits,in the plasma of 45 mild/moderate AD patients and 48 controls.Results Compared with controls,the concentration of ADMA(0.95?0.09?mol/L,P=0.00)was significantly increased,however the activity of NOS((13.9?3.9)U/L,P=0.00),inducible NOS(iNOS)((5.1?1.8)U/L,P= 0.00)and concentration of NO((41.7?5.0)?mol/L,P=0.00)were significantly decreased.In addition,Logistic regression analysis found significant positive correlations between the plasma concentration of NO and the score of the CDR(y=36.64?0.398_x;r=0.398,P

Objective:To analyze the influence factors of postoperative pregnancy in patients of endometriosis associated infertility after laparoscopy combined with hysteroscopy treatment.Methods:340 patients with endometriosis associated infertility who underwent laparoscopy combined with hysteroscopy treatment in Maternal and Child Health Care Hospital of Xiamen from April in 2012 to April in 2014 were followed up for 18 ～ 42 months to assess fertility outcomes.The relative factors of postoperative pregnancy rate were analyzed.Results:85.88％ (292/340) of patients were followed up,and the overall postoperative pregnancy rate was 56.51％.Among which the postoperative pregnancy caseswere 115 cases (39.38％),40 cases (13.70％),8 cases (2.74％),2 cases (0.68％) in the 1 ～ 6 months,7 ～ 12 months,13 ～ 24 months,25 ～ 42 months,respectively.There were significant differences among the four groups(x 2 =11.76,P ＜ 0.05).The multy-factors analysis showed that age ≥35,infertility time ≥3,r-AFS stage relatively high were risk factors of postoperative pregnancy(OR =10.45,3.03,3.74,P ＜0.05),and secondary infertility,applying ovarian stimulation treatment were the protective factors of postoperative pregnancy(OR =0.23,0.33,P ＜ 0.05).Conclusions:Laparoscopy combined with hysteroscopy treatment can increase postoperative pregnancy rate,and the best time to pregnancy is the first one year after treatment.The age ≥35,infertility time≥3,r-AFS stage relatively high are the important causes for the postoperative infertility,while applying ovarian stimulation treatment can promote pregnancy rate.

Objective To explore in vitro effects and the mechanism for FLAG regimen compared with IA regimen in P-glycoprotein-positive and -negative acute myeloblastic leukemia(AML) cell lines. Methods The expression of P-glycoprotein in K562 and K562/A02 cells were analyzed by flow cytometry. The effects of FLAG and IA on the proliferation of K562 and K562/A02 cells were detected by MTT assay. The Ara-CTP and Ara-C levels in those cells were measured by HPLC,the gene expression of hENT1 in K562 and K562/A02 cells was detected by real-time PCR. Results The positive rates of P- glycoprotein were (1.32±0.24)% in K562 cell and (97.66±3.77)% in K562/A02 cell, respectively. The expression of P- glycoprotein had no change after treated with FLAG or IA. The cytotoxicity to K562 of IA was better than FLAG [(84.41±9.33) % v.s (73.17±13.20)%, P<0.05], and the cytotoxicity to K562/A02 of FLAG was better than IA [(70.55±11.32)% v.s (48.46±12.81)%, P<0.01]. Ara-C and Ara-CTP accumulation and hENT1 expression in AML cells treated with FLAG were higher than that treated with IA. Conclusion P-glycoprotein-positive AML cells are more sensitive to FLAG regimen than IA regimen. The biochemical modulation of Ara-CTP and Ara-C may be the major mechanism.

ObjectiveTo summarize the curative effect of l aparoscopic cholecys tectomy (LC) combined with endoscopic retrograde cholangiopancreatography (ERCP) and endoscopic sphincterotomy (EST) for cholelithiasis concurrent with choledoc holithiasis.MethodsERCP and EST were applied in 210 patients suspected for c holedocholithiasis before (164 cases) or after (46 cases)LC.Results ERCP dis closed choledocholithiasis in 69 patients before LC, and then stones were comple tely removed by EST in 63 patients, with a clearance rate of 91 3% (63/69); ERC P revealed residual cholelith in 9 patients after LC, and 8 of them were cured by EST, with a clearance rate of 88 9% (8/9).ConclusionsLC co mbined with ERCP and EST for cholelithiasis concurrent with choledocholithiasis is safe and effec tive, keeping patients free from open surgery.

This paper aimed to introduce and review the antioxidant and material base of selaginella medicinal plants.On the basis of the systematic identification of selaginella medicinal plants,we found that the ethyl acetate extract and flavonoids could inhibit the activity of XOD,LOX or/and COX-2 to some extent.Fourteen biflavonoids,one flavonoid and five flavonoid glycosides have been isolated from three resource plants,among them five compounds were firstly reported and fifteen compounds were firstly isolated in selaginella plants.Amentoflavone (AME),robustaflavone and robustaflavone-4''-dimethylether are three common characteristic constituents of selaginella plants,which can act as marks of common active ingredients and be applied to evaluate the quality of selaginella.Further studies found that some plants had stronger anti-Coxsackie virus and anti-tumor effects,and their mechanisms were associated with inhibiting multiple oxidase and mitigating harm of free radicals due to whole function.