Objective To investigate the distribution of vitamin D receptor(VDR) gene BsmI and Tru9I site gene polymorphism in children in Guangxi region.Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and DNA sequencing technology were used to analyze the VDR genotype in 268 healthy children [including 143 boys and 125 girls which aged (4.15?0.63) years] in Guangxi region.Hardy-Weinberg balance analysis was used to check up the samples.Results There were 246 cases which were bb genotgpes in BsmI site and 21 cases which were Bb genotypes and 1 case which was BB genotypes in 268 cases.The frequency of bb,Bb and BB in BsmI site were 91.79%,7.84% and 0.37%,respectively.Frequencies of b and B allelic gene were 95.71% and 4.29%;There were 12 cases which were tt genotypes in Tru9I site and 85 cases which were Tt genotypes and 171 cases which were TT genotypes in all 268 cases.tt,Tt and TT in Tru9I site were 4.48%,31.72% and 63.80%,respectively.Frequencies of t and T allelic genes were 20.34% and 79.66%.The result of PCR-RFLP was according to the result of DNA sequencing.The samples had group representation through Hardy-Weinberg balance analysis.Conclusions The polymorphism frequency and distribution of VDR gene BsmI and Tru9I site gene polymorphism of children in Guangxi region of China exhibit its own characteristics.BB and tt genotypes minority.

Idiopathic thrombocytopenic purpura(ITP) has been confirmed a clinical syndrome because platelets are much destroyed by immunologic mechanism,it is also called immunethrombocytopenic purpura.The pathogenesis of that is very multiplicity,and at present it isn′t understood.This review article is about study progress of B lymphocyte abnormal and the possible pathogenesis of ITP in children.

Objective To study the glucose-6-phosphate dehydrogenase(G6PD)genotypes in the children of viral hepatitis with G6PD deficiency and investigate the relationship between G6PD deficiency and viral hepatitis.Method DNA samples of 18 children with viral hepatitis/ G6PD deficiency were studied for the 3 common mutations by using the natural primers or mismatched primers mediated PCR followed by restriction enzyme analysis and their clinical manifestation was analyzed.Results Among 18 cases,8 cases were G1388A,and 4 cases were G1376T,and 1 case was A95G.The 3 mutations of G1388A,G1376T and A95G added up to 72.22%.There were no significant difference between the patients with viral hepatitis/ G6PD deficiency and the children only with G6PD deficiency.The incidence rates of acute hemolysis and acute renal failure in the viral hepatitis/G6PD deficiency patients were higher than those in children with G6PD deficiency only.Conclusions It is supposed that the G6PD deficiency is primary in the patients of viral hepatitis with G6PD deficiency.The children of viral hepatitis with G6PD deficiency have more severe pathogenetic conditions and more complications than the children with G6PD deficiency only.

Adolescent ; Adult ; Aged ; Female ; Humans ; Immunophenotyping ; Leukocyte Common Antigens ; immunology ; Male ; Middle Aged ; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma ; immunology ; Young Adult

Child, Preschool ; Heart Septal Defects, Ventricular ; complications ; Humans ; Leriche Syndrome ; complications ; Male

?AlM: To investigate the clinical characteristics and influencing factors of chronic renal failure ( CRF) patients with dry eye, and to provide clinical reference.?METHODS:Sixty-one cases (122 eyes) of patients with CRF ( CRF group ) and 61 cases ( 122 eyes ) of healthy persons ( control group) were carried out on Schirmer ▏test ( S▏t ) , break-up time of tear film ( BUT ) , corneal fluorescein staining ( FL) , test results of two groups were compared and related factors of dry eye in CRF patients were analyzed.?RESULTS: The results of S▏t and BUT in CRF group were lower than that in the control group (P<0. 05). The proportion of tear secretion reduce in CRF group ( S▏t<10mm/5min) was 49. 2% ( 60/122 ), which was higher than that in the control group ( 10. 0%, 12/122 ), the difference was statistically significant ( X2 = 45. 39, P <0. 05). The percentage of instability of tear film in CRF group (BUT≤10s) was 75. 4% (92/122), which was significantly higher than that in the control group (27. 0%, 33/122) (X2=57. 1, P<0. 05). The positive rate of corneal FL was 37. 7% (46/122), which was higher than that of the control group (10. 7%, 13/122), there was a statistically significant difference (X2= 24. 34, P<0. 05).?CONCLUSlON:CRF patients with a decrease in tear film stability and tear secretion are susceptible population to dry eye, clinically should be paid attention to the treatment.

Objective To compare the effect of allograft and autograft of bone-patellar tendon- bone (B-PT-B) under arthroscopy in reconstruction of anterior cruciate ligament (ACL).Methods The injured ACL was reconstructed under arthroscopy by using autograft of B-PT-B in 30 cases and al- lograft of B-PT-B in 28 cases from January 2000 to August 2003.After over twelve months follow up,a comparison was done on rehabilitation of quadriceps femoris,knee stability,the Lysholm's score of knee and the enlargement of tunnel in both groups.Results Both groups obtained marked improvement of knee stability and symptoms after operation.But in the early time,the rehabilitation of quadriceps femoris in the ailograft group is faster than that in the autograft group.The rehabilitation of quadriceps femoris, knee stability and the Lysholm's score of knee were similar at 12th month (P＞0.05).There could be seen enlargement of tunnel in both groups,with more in the allograft group (P＞0.05).Conclusion For arthroscopic reconstruction of anterior crucial ligament (ACL),allograft of B-PT-B is good substitute, for it is characterized by effectiveness,convenience and minor side effect.

Objective To investigate the therapeutic effects of curcumin(Cur)on trinitrobenzene sulphonic acid(TNBS)induced colitis and to investigate cytokines change in colon mucosa,spleen and sera.Methods Colitis was induced in SD rats by intrarectal injection of TNBS(100 mg/kg).Experi- mental animals were divided into negative control group,TNBS group and Cur therapeutic group(Cur, 30 mg?kg~(-1)?d~(-1),intraperitoneal injection).Expression of cytokines mRNA in colon mucosa was observed by RT-PCR,intracellular cytokines of interferon(IFN)-?and interleukin(IL)-4 in splenocytes were detected by flow cytometry(FCM),concentrations of IFN-?and IL-4 in sera were determined by enzyme-link immunosorbent analysis(ELISA).Results After treatment with Cur,macroscopic scores (3.9?1.0 vs.2.2?0.7),myeloperoxidase(MPO)activity(15.0?2.6 vs.7.3?1.4),mRNA of IFN-?(1.02?0.07 vs.0.06?0.02,mRNAof IL-12(0.29?0.05 vs.0.11?0.01)and the ratio of IFN-?/IL-4(11.44?0.97 vs.0.38?0.10)in colon mucosa,proportion of IFN-?CD4~+(31.7?7.5 vs. 21.1?3.7)and the ratio of IFN-?/IL-4 CD4~+(19.9?5.1 vs.6.1?1.8)in splenocytes,concentra tions of IFN-?[(1528?159)pg/ml vs.(513?14)pg/ml] and the ratio of IFN-?/IL-4(19.5?4.1 vs. 4.2?0.6)in sera were all decreased(P＜0.05 or P＜0.01).Meanwhile,mRNA of IL-4(0.09?0.01 vs.0.15?0.04)and IL-10(0.28?0.08 vs.0.63?0.12)in colon mucosa,proportion of IL-4 CD4~+ (1.6?0.5 vs.3.4?1.1)in splenocytes and concentrations of IL-4 in sera[(81?15)pg/ml vs.(124?20) pg/ml] were all increased after the treatment of Cur(P＜0.05 or P＜0.01).Conclusions Cur showed ther- apeutic effects on TNBS-induced colitis,and the mechanism might be through regulating the balance of Th1/ Th2 in colon locally and systematically.

Adult ; Aged ; Antigens, CD20 ; metabolism ; Diagnosis, Differential ; Female ; Gene Rearrangement, B-Lymphocyte, Heavy Chain ; Humans ; Immunohistochemistry ; Leukocyte Common Antigens ; metabolism ; Lymphoma, B-Cell ; genetics ; metabolism ; pathology ; surgery ; Lymphoma, Extranodal NK-T-Cell ; pathology ; Lymphoma, Large B-Cell, Diffuse ; genetics ; metabolism ; pathology ; surgery ; Male ; Middle Aged ; Tonsillar Neoplasms ; genetics ; metabolism ; pathology ; surgery ; Tonsillectomy ; Tonsillitis ; pathology ; Young Adult

Objective:To investigate the distribution of neural cholinesterase and elastic and collagen fibers in rat pancreatic tissues.Methods:Rats were decapitated and the pancreatic tissues were harvested,frozen and made into 8?m serial sections.A combined-staining method(K-R-VB-P-PA method,a combination of Karnovsky-Roots and Victoria blue-Ponceau-Picric acid) was used for staining and the distribution of neural cholinesterase and elastic and collagen fibers was observed.Results:The results showed that the neural cholinesterase was brown.the elastic fibers were cyan.the collagen fibers were light red.and the smooth muscle was yellow in the pancreatic acinar,islet of Langerhans and blood vessels.Conclusion:K-R-VB-P-PA combined staining method can demonstrate the distribution of neural cholinesterase,elastic and collagen fibers and smooth muscle fibers in the pancreatic tissues synchronously.