5.Assessment of quality of life after multiple arthroplasty
Jian LIU ; Jian WU ; Yong DOU ; Ming LV ; Jing TANG ; Yixin ZHOU
Journal of Peking University(Health Sciences) 2015;(2):285-288
Objective:To evaluate the effectiveness of conducting multiple arthroplasty to treat multiple joints disease in terms of quality of life ( QOL) and function improvement.Methods:We compared our results with the reported results of single and dual arthroplasty to see if there is any improvement in QOL, functional scores or complications.In this study, 13 patients admitted to Department of Adult Reconstruc-tive Surgery, Beijing Jishuitan hospital from 2005 to 2009 were included.Questionnaires SF-36 were used to evaluate the QOL.Harris hip score, American Knee Society Score ( KSS) were used to evaluate the joint function.The patients were evaluated before surgery to the latest follow up.Results:SF-36 has changed as follow:physical function 4.17 ±14.43→65.83 ±24.76, role physical 25.00 ±26.11→60.42 ±45.8, bodily pain 23.83 ±21.41→76.88 ±20.89, general health 53.33 ±33.87→76.67 ± 14.67, vitality 50.42 ±17.25→71.67 ±16.28, social functioning 29.17 ±33.50→73.96 ±33.90, role emotional 22.08 ±35.61→77.77 ±41.03, mental health 53.33 ±25.70→82.67 ±14.41, which indicated that they all improved greatly after the surgery ( P <0.05 ) .Harris score increased from 37.68 ±14.71 before the surgery to 83.36 ±13.54 after the surgery.KSS has also showed sharp im-provement (P<0.001) in both clinical score (42.52 ±23.83→77.74 ±20.67) and function score (-2.61 ±22.56→65.65 ±30.76).Conclusion:Multiple arthroplasty is one of the most effective me-thods which can markedly improve the quality of life in patients with multiple joints disease.But compli-cations are common and joint functions are relatively poor.
6.Cloning and expression analysis of a hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferasegene(IiHCT) from Isatis indigotica.
Hong-ran DONG ; Jian YANG ; Lu-qi HUANG ; Jing-ming JIA ; Jin-fu TANG
China Journal of Chinese Materia Medica 2015;40(21):4149-4154
Based on the transcriptome data, we cloned the open reading frame of IiHCT gene from Isatis indigotica, and then performed bioinformatic analysis of the sequence. Further, we detected expression pattern in specific organs and hairy roots treated methyl jasmonate( MeJA) by RT-PCR. The IiHCT gene contains a 1 290 bp open reading frame( ORF) encoding a polypeptide of 430 amino acids. The predicted isoelectric point( pI) was 5.7, a calculated molecular weight was about 47.68 kDa. IiHCT was mainly expressed in stem and undetectable in young root, leaf and flower bud. After the treatment of MeJA, the relative expression level of IiHCT increased rapidly. The expression level of IiHCT was the highest at 4 h and maintained two fold to control during 24 h. In this study, cloning of IiHCT laid the foundation for illustrating the biosynthesis mechanism of phenylpropanoids in I. indigotica.
Acyltransferases
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chemistry
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genetics
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metabolism
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Amino Acid Sequence
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Cloning, Molecular
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Gene Expression Regulation, Plant
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Isatis
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chemistry
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classification
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enzymology
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genetics
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Models, Molecular
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Molecular Sequence Data
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Open Reading Frames
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Phylogeny
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Plant Proteins
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chemistry
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genetics
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metabolism
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Quinic Acid
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metabolism
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Sequence Alignment
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Shikimic Acid
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metabolism
7.Protective Effect of Danxiong Recipe on Brain in Hypoxia Mice
Yi CHEN ; Xiaorong LI ; Jian GAO ; Yu TANG ; Mingming WANG ; Ming XUE ;
Traditional Chinese Drug Research & Clinical Pharmacology 2000;0(05):-
Objective To investigate the protective effect of Danxiong Recipe (DR) on brain in hypoxia mice.Methods Sixty mice were equally randomized to 6 groups:normal group,model group,propranolol group and low-,model- anti high-dose DR groups.Except the normal group,the mice in other groups were given the corresponding drug intra- gastrically according to the experimental design,once a day,for 7 days.After treatment,the survival time under nor- mobaric hypoxia condition in different groups was calculated.Meanwhile,the activities of superoxide dismutase(SOD) and nitric oxide synthase (NOS),and the contents of malondiadehyde (MDA) and lactic acid (LD) in the brain ho- mogenate were detected with test kit.Results The results showed that DR could prolong the survival time of the mice, inhibit obviously the abnormal increase of MDA and LD,reduce NOS activity,and enhance SOD activity in mice brain. Conclusion DR exerts protective effect on brain in hypoxia mice under normal pressure.
8.Heterologous Expression of Zygosacharomyces rouxii ZrGpd 1 in Pichia farinosa
Jie ZHANG ; Xian-Zhong CHEN ; Wei SHENG ; Xue-Ming TANG ; Ge-Jian ZHU ;
China Biotechnology 2006;0(08):-
To examine the effects of heterologous expression of ZrGPD1 (encoding glycerol 3-phosphate dehydrogenase ) cloned from osmotolerant yeast Zygosacharomyces rouxii on glycerol production in wild Pichia farinosa,the URA3 gene was amplified from P. farinosa as the homology integrative region. A recombinant plasmid (pUR-ZG) was constructed then transformed into P. farinosa by electroporation. The transformant pfa-gu was obtained by the selectable marker Zeocin TM . Primary results showed that the biomass of pfa-gu was higher than the wild type in the flask and after 72h fermentation the concentration of glycerol of pfa-gu was 37g/L enhanced 30% in comparison with the wild type. It is concluded that heterologous expression of ZrGPD1 is useful for increasing glycerol production and the ability of osmoregulation in P. farinosa.
9.Construction Fermentation of Engineered Strain and Properties of Recombinant ?-glutamyltranspeptidase
Na WANG ; Jie ZHANG ; Wei SHEN ; Xue-Ming TANG ; Ge-Jian ZHU ;
China Biotechnology 2006;0(11):-
The ?glutamyltranspeptidase encoding gene(ggt) from Bacillus subtilis SYU 20016 was amplified by PCR. The ggt gene was inserted in pBV220 to yield the recombinant expression vector pBV220ggt. Overexpression of ggt in E.coli JM109 was achieved with pBV220ggt. SDSPAGE analysis showed an overexpressed recombinant product at about 65kDa,consistent with the molecular weight predicted from gene sequence. The ferment conditions of r-glutamyltranspeptidase were also discussed. The optimum temperature and pH for the enzyme were determined as 30℃ and 7.2 respectively.The cultures were incubated at 42℃ for 4h with broth volume 20ml/250ml flask and the yield of 6U/ml was obtained, enzyme activity of B. subtilis NX2 was only 3.2 U/ml.
10.Vasculitic lesion in idiopathic IgA nephropathy
Tang JIANG ; Jian-Zhong ZHOU ; Wei-Ming GUAN ; Wen-Xing PENG ;
Chinese Journal of Nephrology 1997;0(06):-
Objective To analyze the pathological and clinical characteristics of patients with idiopathic IgA nephropathy accompanied by vasculitic/crescentic lesion (IgA-V/C). Methods Data of 222 patients diagnosed as idiopathic IgA nephropathy by renal biopsy, among them 87 cases with vasculitic/crescenlic (V/C)lesion, from our department in 2004 were analyzed retrospectively. Clinical and pathological data from patients with IgA-V/C were compared to those of non-IgA-V/C patients and of lupus nephritis (LN) patients with V/C lesion. Results Vasculitic/crescentic lesion was found in 39.19% (87/222) patients with idiopathic IgA nephropathy.And about( 14.08?12.75)% of the glomeruli was affected. It should be taken into account that there was no significant differences of clinical manifestations including blood pressure, urinary protein excretion between IgA-V/C group and non-IgA-V/C group .except serum creatinine(Scr)level which was significantly higher in IgA-V/C group. In addition, only 37.9% of IgA-V/C patients presented high Scr level,thus the lesion of V/C in idiopathic IgA nephropathy was easily overlooked. Patients with idiopathic IgA nephropathy were found to have higher percentage of glomerular sclerosis (64.86% vs 40.00%) and ratio of sclerostic glomeruli to total glomeruli [ (26.98 ?24.68)% vs (16.10 ?18.80)% ]as compared to LN group, which further predicated the progressing characteristics of IgA nephropathy. Conclusions Vasculitic/crescentic lesion is a quite common finding in idiopathic IgA nephropathy and often associated with no dramatically symptoms. It is possible for vasculitic/crescentic lesion to induce unmarked lose of nephron slowly and continually, so as to accelerate IgA nephropathy progression to end-stage renal failure.