Acupuncture Points ; Acupuncture Therapy ; Adult ; Combined Modality Therapy ; Exercise Therapy ; Female ; Humans ; Male ; Middle Aged ; Tennis Elbow ; therapy ; Young Adult

For the purpose of exploring the teaching reform model and method, also promoting the quality of talents in acupuncture and tuina field, the research-based teaching model is applied into the course of Theory of Meridians and Acupoints. This method includes two parts of teaching and learning. For teachers, they bring modern research focus and trend into teaching through questionnaire survey among students, aiming to integrate the education inside and outside class. For students, they are guided to resolve the opening, enlightening and scalable issues through consulting abundant resources of literature and database to achieve autonomous participation in the course of learning. By this teaching method, it is expected to train the student's ability of expanding their thinking, as well as discovering, analyzing and solving the problem.
Acupuncture ; education ; methods ; Acupuncture Points ; Acupuncture Therapy ; methods ; psychology ; Humans ; Learning ; Meridians ; Musculoskeletal Manipulations ; education ; methods ; psychology ; Students, Medical ; psychology

OBJECTIVETo study FANCA protein expression in Fanconi anemia patient's (FA) cells and explore its function.

METHODSFANCA protein expression was analyzed in 3 lymphoblast cell lines derived from 3 cases of type A FA (FA-A) patients using Western blot. Nucleus and cytoplasm localization of FANCA protein was analyzed in one case of FA-A which contained a truncated FANCA (exon 5 deletion). The FANCA mutant was constructed from the same patient and its interaction with FANCG was evaluated by mammalian two-hybrid (M2H) assay.

RESULTSFANCA protein was not detected in the 3 FA-A patients by rabbit anti-human MoAb, but a truncated FANCA protein was detected in 1 of them by mouse anti-human MoAb. The truncated FANCA could not transport from cytoplasm into nucleus. The disease-associated FANCA mutant was defective in binding to FANCG in M2H system.

CONCLUSIONSFANCA proteins are defective in the 3 FA-A patients. Disfunction of disease-associated FANCA mutant proved to be the pathogenic mutations in FANCA gene. Exon 5 of FANCA gene was involved in the interaction between FANCA and FANCG.

Cell Line ; Child ; Exons ; Fanconi Anemia ; genetics ; metabolism ; Fanconi Anemia Complementation Group A Protein ; genetics ; metabolism ; Humans ; Lymphocytes ; metabolism ; Male ; Mutation

Objective To study the effect of early rehabilitation with full range of music therapy on acute coronary syndrome (ACS) patients after emergency PCI. Methods From July to December 2009, 108 inpatients undergone emergency PCI were randomly divided into experimental group and control group, with 54 patients in each group. The experimental group received full range of music therapy, which included PCI treatment under the circumstance of music and music therapy 3 times per day (10:00 a. m. , 16:00 p. m. and 21:00 p. m. ) during hospitalization. The control group received conventional treatment. SBP, HR, the left ventricular ejection fraction (LVEF) , 6-MWT, length of stay, the scale of anxiety and depression were compared between the two groups. Results The scores of anxiety and depression, SBP, HR, LVEF and 6-MWT in the experimental group were significantly different from those of the control group ( P < 0. 05). Conclusions Full range of music therapy significantly contributes to the rehabilitation of ACS patients after emergency PCI.

Objective To discuss the influence of sleep quality on hypertension patients with dysmnesia.Methods Hypertension patients from Department of Cardiology and Hypertension in Tangshan Gongren Hospital from September to December 2011 were chosen by convenience sampling.Self-designed questionnaire was used to collect their data,Pittshurge sleep Quality Index (PSQI) and the rivermead behavicural memory test second edition (RBMT-Ⅱ) were used to assess their sleep quality and memory function.After assessment,patients with dysmnesia were included in the observation group,and patients with normal memory function were included in the control group.The effect of sleep quality on hypertension patients with dysmnesia was analyzed by logistic regression analysis.Results 60％ hypertension patients with dysmnesia had poor sleep quality,and only 25％ hypertension patients with normal memory function had poor sleep quality.The score of PSQI was much higher in the observation group than in the control group [(10.23 ±4.0) vs (5.88 ±1.60)],and the differences was statistically significant (t =6.293,P ＜0.01).Single factor logistic regression analysis showed that hypertension patients with poor sleep quality had 4 times higher risk of dysmnesia than those who had normal sleep quality (OR =4.059,95％ CI =1.568-10.510).Conclusions Poor sleep quality was a risk factor of dysmnesia to hypertension patients,therefore improving sleep quality in hypertension patients is helpful to ease their decrease of memory function.

The transforming growth factor β1 (TGF-β1) and CD8-positive T cells are two important immune factors that function at opposite directions. The purpose of this study was to verify the relationship between the two factors and their associations with long-term effects of adjuvant chemotherapy or endocrine therapy in breast cancer. Expression of TGF-β1 precursor and CD8 was immunohistochemically detected on surgically-obtained tumor samples of 130 (stage I-III) invasive breast carcinomas from Chinese subjects, who were followed up for a mean time of 112 months. Interstitial CD8-positive cells and TGF-β1 precursor-positive cells adjacent to tumor nests were counted. Infiltration of CD8-positive lymphocytes into tumor nests and TGF-β1 precursor expression in tumor cells were observed and survival analysis was performed. Our results showed that density of interstitial CD8-positive lymphocytes was an independent adverse prognostic factor for distant disease-free survival (DDFS) (HR=8.416, 95% CI=1.636-43.292, P=0.011) in hormone receptor-positive patients who were on adjuvant endocrine therapy. For breast cancer patients who did not receive adjuvant chemotherapy, those without infiltration of CD8-positive cells into tumor nests had a shorter overall survival (OS) than their counterparts with CD8-positive cell infiltration into tumor nests (Log-Rank, P=0.003). But OS of patients without infiltration of CD8-positive cells into tumor nests was significantly prolonged by adjuvant chemotherapy (Log-Rank, P=0.013) and paralleled that of patients with CD8-positive cell infiltration. Although OS was shorter in the tumor cell TGF-β1 precursor (t-TGF-β1-pre)-positive patients than in the negative patients in patients without receiving chemotherapy (P=0.053), OS of t-TGF-β1-pre-positive patients was significantly prolonged by adjuvant chemotherapy (P=0.035) and was longer than that of t-TGF-β1-pre-negative patients. Analysis showed that t-TGF-β1-pre was an independent positive prognostic factor for DDFS (HR=0.392 95% CI=0.157-0.978, P=0.045) in patients who received adjuvant chemotherapy. This study suggested that density of interstitial CD8-positive lymphocytes was of prognostic value in hormone receptor-positive patients who received adjuvant endocrine therapy. Our study verified that adverse immunologic signatures consisting of absence of CD8-positive cells in tumor nests or expression of TGF-β1 precursor in tumor cells in breast cancer were associated with worse prognosis and significantly improved long-term survival with adjuvant chemotherapy, respectively.
Biomarkers, Tumor ; metabolism ; Breast Neoplasms ; drug therapy ; metabolism ; surgery ; CD8-Positive T-Lymphocytes ; metabolism ; Chemotherapy, Adjuvant ; Combined Modality Therapy ; Female ; Humans ; Immunohistochemistry ; Kaplan-Meier Estimate ; Middle Aged ; Outcome Assessment (Health Care) ; methods ; statistics & numerical data ; Prognosis ; Proportional Hazards Models ; Protein Precursors ; metabolism ; Retrospective Studies ; Transforming Growth Factor beta1 ; metabolism

OBJECTIVETo investigate the acute toxicity and assess the median lethal dose (LD50) of matrine in Kunming mice.

METHODSMatrine at different doses were administered in Kunming mice via intraperitoneal injection, and the toxic reactions and LD50 of matrine was observed and determined.

RESULTSThe acute toxicity test of matrine indicated that the tolerable dose of matrine was above 80 mg/kg in Kunming mice, and the LD50 was 157.13 mg/kg (95%CI, 88.08-280.31 mg/kg). Morphological observation revealed degenerative changes of the nerve cells in the brain tissue of the mice.

CONCLUSIONThe nervous system is the main target organ by the toxicity of matrine.

Alkaloids ; toxicity ; Animals ; Brain ; drug effects ; pathology ; Female ; Lethal Dose 50 ; Male ; Mice ; Quinolizines ; toxicity ; Toxicity Tests, Acute

Objective To discuss the video analysis basing on emergency process,and combined with the application effect on PBL(Problem based learning)teaching model in trauma simulation teaching for emergency undergraduate intern students. Methods There were 120 undergraduate intern students, coming to the hospital from January to December in 2016,been selected as subjects.The single month was the control group,and the bimonthly was observation group.Each group contained of 60 members.In the first week, two groups focused on the theoretical knowledge of wound treatment, emergency treatment process and watched the trauma disposing video. In the second and third weeks, followed the rotation of one to one clinical teaching methods. In the fourth week, the control group took regular simulation exercises. Teachers might intercept a case of rescuer video and use PBL teaching mode to guide the observation group. Students would discuss and use the first aid process analysis rescue video in 3-5 persons a group. And then compared with two groups in the theoretical score, simulation exercises performance and the evaluation of teaching results. Results After the teaching program,the theoretical score of observation group was 89.17±5.55,and the control group was 82.72±4.92(t=6.742,P<0.01).The simulation exercises score of observation group was 88.62±5.64,and the control group was 74.25±7.68(t=11.681, P<0.01). The evaluation score of teaching results of observation group was 91.55±4.32, and the control group was 83.64 ± 3.54 (t=10.973, P<0.01). The difference between two groups was statistically significant. Conclusion The use of the first aid process of video analysis combined with PBL teaching model can improve the trauma of the nursing students first aid ability,stimulate students'interest in self-study,and improve the ability of first aid and solving problems

OBJECTIVE: To investigate the effect of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase, on the activation of hepatic stellate cells (HSCs) and its mechanism. METHODS: Primary HSCs isolated from SD rats were cultured and treated with different concentrations (1, 3 or 10micromol/L) of ADMA for various periods (12 approximately 48h). Expression of alpha-smooth muscle actin (alpha-SMA) and synthesis of type-I collagens in HSC were determined. Messenger RNA levels of the transforming growth factor-beta1 (TGF-beta(1)) in the HSCs were determined using RT-PCR. Intracellular reactive oxidant species (ROS) production was measured using oxidant-sensitive fluorescent indicator. Activation of nuclear factor-kappaB (NF-kappaB) was detected by electrophoretic mobility shift assay (EMSA). RESULTS: ADMA could increase alpha-SMA-positive cells ratio and Type I collagens production of HSCs in a concentration- and time-dependent manner, concomitant with the increase of the TGF-beta(1) mRNA level. Treatment with ADMA (10micromol/L) significantly increased the intracellular ROS production and activated NF-kappaB. Such effects of ADMA on the level of TGF-beta(1) mRNA could be markedly attenuated by pretreatment with antioxidant pyrrolidine dithiocarbamate (25micromol/L). CONCLUSION ADMA can induce the HSC activation by increasing TGF-beta(1) expression through ROS-NF-kappaB-dependent pathway. Therefore, ADMA should be a novel and endogenous activator of HSC, which may be involved in the development of liver fibrosis.
Actins ; biosynthesis ; Animals ; Arginine ; analogs & derivatives ; pharmacology ; Cells, Cultured ; Collagen Type I ; metabolism ; Dose-Response Relationship, Drug ; Gene Expression ; drug effects ; Hepatocytes ; cytology ; drug effects ; metabolism ; Male ; NF-kappa B ; metabolism ; Nitric Oxide ; metabolism ; Nitric Oxide Synthase ; metabolism ; RNA, Messenger ; genetics ; metabolism ; Rats ; Rats, Sprague-Dawley ; Reactive Oxygen Species ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Transforming Growth Factor beta ; genetics

Objective To estimate the incidence,mortality and 5-year prevalence rates of lung cancer in China,in 2008.Methods Data from 36 cancer registries and the Third National Death Survey in China (2004-2005) was used to estimate the incidence,mortality and 5-year prevalence rates of lung cancer in China in 2008.Mathematical models were used to predict the lung cancer incidence and mortality rates in the next 20 years.Results In 2008,the incidence of lung cancer was 522 050 ( 18.5％ ) with the incidence rate as 33.5/100 000,which ranked the first among all the cancers.Mortality of lung cancer in China was 452 813 (23.1％) with the mortality rate as 28.7/100 000,which also ranked the first among all the cancers.The 5-year prevalence rate of lung cancer in China was 487 815 (10.6％) with the proportion as 45.6/100 000,which ranked fourth among all the cancers.Lung cancer happened more frequently among people older than 45 years,particularly in males.Our data on prediction showed that the incidencc and mortality of lung cancer in China would gradually increase in the next 20 years.Conclusion Lung cancer was the leading cause for both incidence and mortality of all canccrs in China and both kept increasing.The key population fell in those older than 45 ycars,particularly males,that should be under special prevention and control for lung cancer.