Objective To analyse the effects of OPG (osteoprotegerin, OPG) at different concentrations on activity of osteoclasts (osteoclasts, OC) , and the relationship between them in physiological activity and function.Methods The bone marrow cells of six weeks old female mice were collected and cultured in vitro on the basis of adding different levels of OPG(10, 20, 50, 100 μg/L).The relationship between OPG and OC were analysed by tartrate resistant acid phosphatase ( TRAP) staining, F-actin cytoskeleton staining and bone resorption pits.Results OC showed adherent growth within 1h cultured, and there were no large multicore OC.Cultured for 5 days, the smaller monocytes gradually began to fusion with each other to form multinucleated cells and showed distinctive characteristics.After three days OPG treatment, the number of multicore cells gradually reduced with OPG concentration increaseing in experimental group, and showing a negative correlation between them(r=-0.516,P<0.05).And there were only a small amount of multinucleated cells at OPG concentration of 50 μg/L.At 20,50 μg/L of OPG concentration, OC number significantly decreased compared with control group (P<0.05), and when the concentration of OPG was 100μg/L, OC number showed significant reduction (P<0 01).the experimental group OPG bone resorption lacunae area, density and decrease the degree were positively correlated with concentration of OPG (r=0.459;r=0.426;r=0.389,P<0.05), and the area had a significant difference when two groups compared.Conclusion The inhibitive effects of OPG on OC function and activation increase with concentration increasing.

Objective To provide the experimental evidence for clinical application of taurine,rat model of intrauterine growth restriction (FGR) was made to investigate influence of prenatal administration of taurine on neurogenesis.Methods Fifteen pregnant rats were divided into control,FGR model and taurine groups,5 rats for each group.Rats in the control group were supplied with unlimited food and drink while the other two groups were fed by 40％ food intake of the control group throughout pregnancy.Since gestational day 12,taurine (100 mg/kg) was added into diet of taurine group every day until term delivery.Brain tissues were obtained immediately after baby rats were born.Expression of proliferating cell nuclear antigen (PCNA),neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) of brain tissue was measured by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry; meanwhile,numbers of PCNA-immunoreactive cells in subventricular zone,subgranular zone and cerebral cortex were compared with ANOVA test or q test.Results Levels of PCNA mRNA and GFAP mRNA expression in FGR group were significantly higher than those of control group (PCNA mRNA:1.002±0.011 vs 0.894 ± 0.040,P＜0.01; GFAP-mRNA:1.012±0.013 vs 0.913±0.012,P＜0.01).Compared to FGR model group,mRNA expressions of PCNA and GFAP in taurine groups were higher (1.090±0.029,P＜0.01 ; 1.034±0.011,P＞0.05).There was a significant decrease in the expression of NSE mRNA in FGR group compared with control group (0.796±0.036 vs 1.582±0.057,P＜0.01),while the expression in taurine group (0.933±0.027) was significantly higher than that in FGR model group (P ＜ 0.01).PCNA immunoreactive cells were mostly distributed in subventricular zone,followed by subgranular zone and cerebral cortex.Conclusions Prenatal application of taurine could enhance neurogenesis of FGR newborn rats and improve survival of neurons to ameliorate FGR brain damage.

Due to substantial morbidity and high complications, diabetes mellitus is considered as the third "killer" in the world. Medicinal fungal polysaccharides, as water-soluble macromolecular substances with low toxicity, exhibit diversified pharmacological actions such as immune regulation, anti-tumor, antivirus, antioxidant, anti-aging, hypoglycemic effect and improving liver and kidney function. In recent year, a number of investigators reported medicinal fungal polysaccharides showed good anti-diabetes and hypoglycemic activity, and their acting mechanisms involved in glycometabolism and β cell function, e. g. promoting glycogen synthesis, promoting glycolysis, inhibiting the activity of α-glucosidase, promoting insulin secretion, increasing insulin sensitivity, enhancing antioxidation. Therefore, the hypoglycemic activity and its mechanisms of action of medicinal fungal polysaccharides showed characteristics of multiple effects, multi-target, and multi-pathway regulation. These finding suggest that medicinal fungal polysaccharides are a promising source for the development of discovery of anti-diabetic agent.
Animals ; Carbohydrate Metabolism ; drug effects ; Fungal Polysaccharides ; pharmacology ; Humans ; Hypoglycemic Agents ; pharmacology ; Insulin Resistance ; Insulin-Secreting Cells ; drug effects ; Oxidative Stress ; drug effects

Biomedical Research ; Drugs, Chinese Herbal ; therapeutic use ; Evidence-Based Medicine ; Medicine, Chinese Traditional ; methods ; Phytotherapy ; methods

BACKGROUND:Emerging evidence shows that rs6756667 SNP EPAS1-A alele is beneficial for, however, the GG genotype is detrimental for adaptation to high-altitude hypoxia in Tibetan populations.
OBJECTIVE:To compare the EPAS1 gene rs6756667 polymorphism between Tibetan elite endurance athletes and Tibetan non-physical education major students and analyze its relationship to aerobic exercise capacity.
METHODS:Forty Tibetan athletes in Ganzi Tibetan middle and long distance athletes sports teams (athlete group) and forty Tibetan non-physical education major students in Ganzi (student group) were included in this study. The EPAS1 gene rs6756667 polymorphic loci were determined using PCR high-resolution melting analysis. The genetic polymorphism data from this study were compared withthe data from previous studies. Increase values of bone mineral density in the pelvis, upper and lower limbs were determined by dual-energy X-ray absorptiometry.
RESULTS AND CONCLUSION:There were significant differences in the genotype frequencies of EPAS1 rs6756667 AA, AG and GG between student group(50%, 47.5%, 2.5%)and athlete group(72.5%, 27.5%, 0;P< 0.05). The genotype frequency of GG was significantly increased in student group compared with athlete group (P< 0.05). There were differences in the frequencies of A alele and G alele between both groups (95%CI:0.459-0.908,P=0.011).Bone mineral density inthe pelvis, upper and lower limbs and whole body was significantly higher in student group than athlete group (P< 0.05). These findings suggest that EPAS1 gene rs6756667 polymorphism is related to aerobic capacity of Tibetan elite endurance athletes. rs6756667 SNP EPAS1-A alele is beneficial for, however, the GG genotype is detrimental for adaptation to high-altitude hypoxia in Tibetan athletes.

Objective To utilize the expert resource in central hospital for helping doctors in community hospital to make suitable diagnosis and treatment,improve the technical level of community medical care. Methods The Telemedicine Diagnosis System is developed based on B/S mode and Microsoft Visual Studio.net C#2005 in Windows platform. Results The Telemedicine Diagnosis System has been developed, and realized the function of interface display, information management, medical image transmission and telemedicine diagnosis. Conclusion The system has friendly interface, simple operation, more reliability and quick data transmission, and it can accurately realize the experts' telemedicine diagnosis in time.

Objective:To explore the relationships between perfectionism and coping strategies among graduate students.Methods:396 college graduate students completed the COPE (the Coping Inventory),FMPS (Frost's Multidimentional Perfectionism Scale) and General Self-efficacy Scale.Results:①Significant positive correlations were found between adaptive perfectionism and self-efficacy,while negative correlations between doubt about actions in maladaptive perfectionism and self-efficacy;② Regression analysis indicated predictability of perfectionism and self efficacy to coping strategies.Self-efficacy still had significant predictability after perfectionism and its sub-dimension were controlled.Conclusion:Self-efficacy plays an important mediation role between perfectionism and coping strategy.

iple occurrence of the tumor, which can decline its recurrence and postpone its progression.

In 2012,Beijing Children’s Hospital established the Beijing Children’s Hospital Group to explore the new model of public hospital reform.This study covered the target setting,management framework,network system,and sharing mechanism of the group,and discussed the merits and setbacks of such a practice,for insights of public hospital reform.

AIM To observe the effects of Wuwei Wentong Chubi Capsules (Cinnamomi Ramulus,Poria,Epimedii Folium,etc.) on autophagy proteins of Beclin-1 and LC3-Ⅱ in adjuvant-induced arthritis rats and to explore the possible mechanism of action.METHODS Sixty SD rats were randomized into six groups:normal group,model group,Wuwei Wentong Chubi Capsules (0.8,1.6,3.2 g/kg) groups and tripterygium glycosides (TPT,40 mg/kg) group.In addition to the normal group,adjuvant arthritis (AA) was induced with Freund's complete adjuvant.From the 2th day after injection of FCA,Wuwei Wentong Chubi Capsules with different doses and TPT were given by gavage once a day for 12 days.At the end of the experiment,ankle-joint samples were taken to examine the degree of AA by HE.Beclin-1 and LC3-Ⅱ mRNA were determined by real-time fluorescent quantitative PCR.Meanwhile,the protein expression levels of Beclin-1 and LC3-Ⅱ were determined by immunofluorescence histochemical staining and Western blot.RESULTS As compared with the model group,Wuwei Wentong Chubi Capsules (1.60,3.20 g/kg) not only significantly reduced histopathological injuries,but also effectively up-regulated mRNA and protein expressions of Beclin-1 and LC3-Ⅱ.CONCLUSION Wuwei Wentong Chubi Capsules has a therapeutic action on AA in rats,which might be partly associated with promoting autophagy,decreasing excessive proliferation of synovial cells,leading to the reduction of damage to articular cartilage.