Objective To study the reliability of clinic diagnosis of hepatitis B virus(HBV)-associated glomerulonephritis.Met- hods Renal biopsy was carded out in 103 cases with clinically-diagnosed HBV-associated glomerulonephritis. The diagnostic consistency and clinical difference were evaluated.Results Sixty nine cases with clinically-diagnosed HBV-associated glomerulonephritis were confirmed through renal biopsy, 29 cases were excluded from HBV-associated glomerulonephritis. The pathologic diagnosis and clinical diagnosis methods were highly correlated and has significant difference(P

Objective: To observe the expression of Cox-2 and Ki-67 protein in mucoepidermoid carcinoma (MEC) and normal salivary gland (NSG), so as to search for biomarkers for evaluation of MEC prognosis. Methods: The expression of Cox-2 and Ki-67 protein was examined by PV-6001 staining in 11 NSG and 34 MEC samples, and the experimental data were statistically analyzed by SPSS 16.0 software. Results: The expressions of Cox-2 were significantly different between MEC and NSG samples (P < 0.01). Cox-2 expressions were also significantly different between MEC samples with different differentiation, metastasis and recurrent statuses(P<0.05, P<0.01), and were similar between those with different sexes, ages, or ethnicities(P>0.05). The expressions of Ki-67 were significantly different between MEC and NSG samples (P<0.01). Ki-67 expressions were also significantly different between MEC samples of high- and moderate-differentiation degrees with low-differentiation degrees, and samples with different recurrence, and metastasis statuses and sites(P<0.05, P<0.01), and were similar between the high-differentiation and middle-differentiation samples, and samples with different sexes, ages, and ethnicities. Cox-2 expression was positively correlated with Ki-67 expression in MEC samples (rs=0.788, P<0.01). Conclusion: Cox-2 and Ki-67 expressions in MEC are associated with the pathological types of MEC, and they may participate in the development and progression of MEC. High expression of both is associated with local infiltration and distant metastasis. Co-detection of Cox-2 and Ki-67 is helpful for predication of MEC prognosis.

CCAAT-Enhancer-Binding Proteins ; genetics ; Cytogenetic Analysis ; Cytogenetics ; methods ; Extremities ; Humans ; Liposarcoma ; etiology ; genetics ; Liposarcoma, Myxoid ; etiology ; genetics ; Molecular Biology ; methods ; Oncogene Proteins, Fusion ; genetics ; RNA-Binding Protein FUS ; genetics ; Retroperitoneal Neoplasms ; etiology ; genetics ; Ring Chromosomes ; Soft Tissue Neoplasms ; etiology ; genetics ; Transcription Factor CHOP ; Translocation, Genetic

Objective To investigate the extracellular regulated protein kinases (ERKs) pathway of hepatitis B virus(HBV) X protein(HBx) on glomerular mesangial cell(GMC) proliferation of rat and tumor necrosis factor(TNF)-? expression.Methods The HBV X gene was amplified by polymerase chain reaction(PCR),inserted into the eukaryotic expression vector PCI-neo and confirmed by restrict endonuclease digestion and sequence analysis.PCI-neo contained HBV X gene (PCI-neo-X) was transfected into cultured GMC via liposome.GMC proliferation,TNF-? and its mRNA expression were investigated in the condition of with or without U0126 in the culture media.HBx,ERK1/2 and phosphorynated-ERK1/2 (p-ERK1/2) expression in GMC were assessed by Western blot.TNF-? mRNA expression was assessed by semi-quantitative reverse transcriptase-PCR (RT-PCR).TNF-? level in supernatants was measured by enzymelinked immunosorbent assay(ELISA).GMC proliferation was assessed by methyl thiazolyl tetrazolium(MTT).Results HBx expression was found in transfected GMC,and became prominent at 36 and 48 hours after transfection whether with or without U0126 in culture media.TNF-? mRNA expression was significantly decreased in U0126 group compared with U0126-free group.TNF-? levels in supernatants in PCI-neo-X transfection without U0126 group were (189.0?18.1) ng/L at 36 hours and (172.3?24.3) ng/L at 48 hours after transfection,respectively.In contrast,TNF-? levels in supernatants with U0126 were (65.6?11.6) ng/L and (84.0?24.6) ng/L,respectively.The TNF-? le-vels in the latter groups were significantly lower than the formers (Pa

Objective To explore the clinical features of pulmonary tuberculosis complicated with DM and related factors. Method The manifestations of 87 newly diagnosed PTB patients with type 2 DM(DM-PTB group) at two hospitals in Tianjin during October 2003 to October 2004 were compared to 88 PTB patients without DM who were randomly choosen from the same hosipital.The factors related to sputum-positive TB or cavity were explored through unconditional logistic regression model. Results DM-PTB patients showed significanly lower frequencies of cough(66.7% vs 83.0%),toxicity symptoms(37.9% vs 62.5%) and haemoptysis(16.1% vs 33.0%) than those of PTB group.Positive-sputum(63.2% vs 34.1%),cavity(56.3% vs 13.6%),wide lesions and bilateral lesions on chest radiographs was more common in DM-PTB group than in PTB one.Introversion(OR=1.582, 95%CI:1.063 ～2.355),type 2 DM(OR =2.186,95% CI:1.082 ~4.420)and cavity(OR =2.793,95% CI:1.308 ～5.964)were factors independently associated with positive-sputum TB.The factors related to cavity included diabetes(OR=14.199,95%CI:5.376~ 37.501),cough,postive sputum and lore-up lobe lesion.Conclusion This study confirmed that clinical manifestations and chest radiographs of PTB patients complicated with type 2 DM significantly departed from the typical presentation.Type 2 DM seems to have a negative effect on postive-sputum and cavity.So it should be alarmed that DM-PTB patients who tend to have untypical clinical symptom and higher rate of positive sputum become the reservoirs of infection to cause the transmission of TB.

Genetic polymorphism of properdin factor B (Bf)of alternative complement pathway was investigated in 110 patients with Gravesldisease and in 220 blood donors in Wuhan Blood Transfusion Center using high voltage agarose gel electrophoresis andsubsequent immunofixation. Results show that distributions of Bf phenotype observed in normals are in agreement with those expected from the Hardy-Weivr, erg equilibrium (XZ=5.4337, Pc~0.10),but in patients donlt (XZ=29.689~, Pc.

Objective To establish an evaluation index system for Shanghai urban general practice service team to improve standardized and scientific management. Methods The evaluation index system for Shanghai urban general practice service team was established using two rounds of Delphi consultation. Results The established system consisted of 5 fast-grade indicators and 56 second-grade indicators. The 5 first-grade indicators were staffing, infrastructure, quality and quantity of service, continuing education and satisfaction degree of suppliers and demanders, with weight of 0.2153, 0.1978, 0.2087, 0.1869 and 0.1923 respectively. The active coefficient for two rounds of Delphi consultation was both 100%. The authoritative coefficient of the two rounds was 0.852 and 0.883 respectively. The harmonious coefficient of importance of the evaluation index was 0.251 and 0.411 respectively. Conclusion Through using Delphi method scientifically and reasonably, we set up the evaluation index system for Shanghai urban general practice service team, with integral contents, strong operability and easy application.

Adult ; Base Sequence ; DNA, Neoplasm ; genetics ; Exons ; Female ; Humans ; Liposarcoma ; genetics ; Liposarcoma, Myxoid ; genetics ; Male ; Middle Aged ; Molecular Sequence Data ; Oncogene Proteins, Fusion ; genetics ; Polymerase Chain Reaction ; methods ; RNA-Binding Protein EWS ; genetics ; RNA-Binding Protein FUS ; genetics ; Sequence Analysis, DNA ; Transcription Factor CHOP ; genetics ; Translocation, Genetic