A rapid method for directly determining tryptophan contents in foods and feedstuffs was described. l-5mg dry sample could be completely hydro-lyzed in a teflon tube containing 5N NaOH with 0.5% soluble starch under 145℃ for 4 hours in vacuum (760mmHg). The hydrolysate was then adjusted to neutral with 6N HCL in icebath and diluted by five times with 4M urea solution (pH = 11), and was measured at excitation wavelength 280nm and fluorescence wavelength 368nm. The sensitivity of the method was 5ng /10ml, and the recovery rate of 97-107% were obtained in the range of 1-5 ?g/10ml standard tryptophan. The value of a number of foods and feed-stuffs obtained were in agreement with the literatature values.

Objective To draw up a satisfaction rating scale utilized by outpatients in general hospitals,for the quality evaluation of the out-patient service.Methods Outpatients at four municipal general hospitals in Hangzhou City were as the subjects.The validity and reliability of the rating scale were estimated.Results The response rate to items was 98.82% ,and valid rate reached 96.8% ,the internal consistency coefficient was in the range of 0.76 to 0.87.The composition of the scale conforms well to the management practice,the relativity inside the factor was stronger than the relativity among the factors,which supported the contents validity.Factor analysis results demonstrated that both the factor loads and structures were consistent with the structures of the rating scale,which supported the structure validity.Conclusion All of validity indexes of the rating scale meet the requirements of psychometrics.This scale is practicable to estimate the satisfaction of outpatients to the service they received,and provides valuable evidence to estimate the quality of hospital therapeutic services.

Objective To investigate the effect and mechanism of intravenous bone marrow stromal cells (BMSCs)transplantation against chronic ethanol-induced cognitive dysfunction in rats.Methods BMSCs from rats were isolated,cultured and identified in vitro.Male Sprague-Dawley rats(n=28)were randomly divided into control group(n=7),chronic ethanol group(n=7),BMSCs group(n=7)and PBS group(n=7).BMSCs(1×10~7 cells/animal)or PBS were intravenously injected to the rats of BMSCs or PBS group at the 7th week after ethanol gavage,respectively.All rats were sacrificed at the end of the 8th week.The learning and memory behavior was assessed using Moms water maze task.Immunofluorescence was used to examine cell proliferation maker(BrdU)in hippocampal dentate gyrus.Immunohistochemistry was used to evaluate NGF and BDNF expression in hippocampal region.Results In Morris water maze,escape latency at each time point in BMSCs group((33.83±3.26)s,(13.14±2.64)s,(6.53±0.98)s,(4.45±0.85)s)were significantly shorter than those in chronic ethanol group((53.78±4.29)s,(36.53±2.39)s,(18.28±2.13)s,(8.67±0.46)s,all P<0.01))and time percentage of platform quadrant in BMSCs group((73.99±1.49)%) was dramatically longer than that in chronic ethanol group((51.25±3.69)%),but no significant difference with contro1.The number of BrdU-positive cells in the dentate gyrus was significantly higher in BMSCs group than that in chronic ethanol group(P<0.01).Comparing to chronic ethanol gavage,BMSCs transplantation significantly increased the expression of NGF alld BDNF (P<0.01)in hippocampus.Conclusion Intravenous BMSCs transplantation is able to result in enormous attennation of the learning and memory abnormalities in chonic alcoholism rats,and which might be involved to promote hippocampal endogenous cellular proliferation by secretary neurotrophic factors.

[Objective] To study the effect of anti-infective reconstituted bone xenograft(ARBX)on treating chronic hematogenous osteomyelitis as one-stage grafting.[Method]ARBX was used to treat 24 cases of chronic hematogenous osteomyelitis as one-stage grafting after debridement since September of 2001,17 cases of which were followed up for an average period of 34 months(range,12 to 79 months).[Result]Except 1 case failed to cure,and 1 resulted in recurrence of infection,and 1 with large segmental bone defect nonunion post-operatively,other 14 cases were cured,the cure rate was 82.4% which was better than traditional therapy.[Conclusion]ARBX has high osteoinductive activity and enhanced anti-infective capability,which enables it to be used as primary grafting to treat chronic hematogenous osteomyelitis.

Objective To evaluate the microRNA role in the pathogenesis of alcohol dependence.Methods By computer retrieval Medline,mirbase,PubMed,Chinese journal full-text database (CNKI),ten thousand database and VIP database,manual retrieval relevant references.Literature retrieval time limit are built from the library until July 2014.English database to Alcoholism,Alcohol Dependence,Alcohol Abstinence,Alcohol Amnestic Disorder Fetal Alcohol Spectrum Disorders,mirna as keywords retrieval;Chinese database to Alcoholism,Alcohol Dependence,Alcohol Abstinence,Alcohol Amnestic Disorder Fetal Alcohol Spectrum Disorders,microRNA,etc.Retrieved from the database to the related literature in July 2014.Results A total of 26 studies were recruited including 8 Chinese literatures and 18 English literatures.Conclusion Abnormal expression of miRNAs will become the next research direction.MicroRNAs may become new biomarker of alcohol dependence on diagnosis,may also makes the molecular drug targets,or the molecular simulation for new drug research and development,this could be for the treatment of alcohol dependence provides a new means.

Objective To investigate the changes and clinical significance of serum procalcitonin (PCT) level in children with acute phase of Kawasaki disease (KD). Methods The serum PCT levels and their changes before and after the treatment in 120 children with KD at acute phase were retrospectively analyzed. According to the results of ultrasonic echocardiography, all children were divided into coronary artery damage (CAL) group and no coronary artery lesion (NCAL) group. According to the presence of gastrointestinal symptoms, patients were divided into two groups (A: yes and B: no ). According to the presence of abnormal liver function, patients were divided into two groups (C:yes and D:no). PCT levels were compared between groups. Results The serum PCT levels were increased at acute phase in 56 (46.67%) patients before the treatment than those in normal children, which were decreased obviously after treatment (P<0.05). There were 31 cases combined with CAL, the rising rate of PCT was 38.71%, which was no significant difference compared with that of NCAL group (49.44%, P>0.05). There was no significant difference in serum PCT value between CAL group and NCAL group (P>0.05). The serum C-reactive protein level was significantly higher in CAL group than that of NCAL group. There were 35 patients combined with gastrointestinal symptoms in 120 KD patients. There was no significant difference in the rising rate of PCT between patients with gastrointestinal symptoms (62.86%) and patients without gastrointestinal symptoms (40.00%, P<0.05). There was no significant difference in serum PCT level between these two groups of patients. There were 42 cases with liver dyfunction in 120 KD patients, the PCT rising rate (52.38%) was no statistically significant difference compared with that of patients with no liver dyfunction (43.59%, P>0.05). And there was no significant difference in serum PCT value between the two groups (P>0.05). Conclusion PCT can reflect the acute phase of KD patients. The increased PCT level may be related with the emergence of gastrointestinal symptoms, even though it cannot predict CAL and the occurrence of liver damage.

This study developed a management information system for rural basic publication health. The system, which is convenient for management of public health information and can improve the efficiency, is suitable for management of rural basic public health at various health administration sections.

Objective To study the effect and its mechanism of coronary stenting on blood levels of P-selectin. Methods Thirty patients who received coronary stenting from September 2003 to September 2004 were included in the study and another twenty six patients who received coronary angiography during the same period were selected as controls. Blood samples were drawn at 1 h before and at 1 h, 4 h, 24 h, 48 h, 1 week after coronary stenting. Blood samples were taken at 1 h before and at 1 h, 4 h, 24 h after coronary angiograpy. Results In patients with coronary stenting, P-selectin levels after the procedure were significantly higher than that before the procedure (P

Objective To evaluate the therapeutic effect of Xiaoke Huayu tablet on impaired glucose tolerance (IGT). Methods 168 patients who were proved to have IGT were randomly divided into two groups: treatment group (n=86) and control group (n=82). All the patients were asked to take abstinent diet with proper athletic exercises. The patients in the treatment group were treated with Xiaoke Huayu tablet. No drug was given to patients of the control group. The therapeutic effect and data of laboratory findings before and after treatment in both groups were analyzed and compared. Results There was significant differences before and after treatment between two groups. The total effective rate was 91.8% in the treatment group and 68.3% in the control group (P

Objective: To observe the effect of Weixikang apozema on growth factor of acetic acid gastric ulcer rats.Methods: The rats were divided into blank control group(abbreviated as blank group),model group,ranitidine group,Weixikang low dosage group(abbreviated as low dosage group) and Weixikang high dosage group(abbreviated as high dosage group) at random,each group has 8 rats.The content of epidermal growth factor(EGF) and transforming growth factor-?(TGF-?) were measured with radioimmunoassay.The protein expression of EGF,TGF? and EGFR were measured with immunohistochemical method.Results: The content of EGF and TGF-? and the protein expression of EGF,TGF-? and EGFR in low dosage group and high dosage group have increased(P