2.Apoptosis in Lungs and Liver after Crush Injury of Hindlimbs in Rat.
Jie ZHAO ; Hua-rong WANG ; Jian-heng BU ; Min ZUO ; Guo-zhong ZHANG
Journal of Forensic Medicine 2015;31(2):88-92
OBJECTIVE:
To investigate the process of apoptosis in lungs and liver induced by crushing hindlimbs of rat, and study the mechanism of crush injury.
METHODS:
The rat experimental model of hindlimbs crush injury was established. The cell apoptosis in lungs and liver was detected by TUNEL assay, and the expression of Bax, Bcl-2 and caspase-3 apoptin was examined by immunohistochemistry.
RESULTS:
Compared with the control group, the partial muscle injury of rat's hindlimbs was more serious with more apoptosis observed in lungs and liver (P < 0.05). The expression of Bax was up-regulated and Bcl-2 was down-regulated, whereas caspase-3 expression was activated (P < 0.05).
CONCLUSION
The cell apoptosis has increased significantly in lungs and liver after crush injury of hindlimbs in rat. The correlation factor released during tissue injury may mediate apoptosis process.
Animals
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Apoptosis/physiology*
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Caspase 3/metabolism*
;
Genes, bcl-2
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Hindlimb/injuries*
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Immunohistochemistry
;
Liver/physiopathology*
;
Lung/physiopathology*
;
Rats
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Up-Regulation
;
bcl-2-Associated X Protein