Objective To investigate the clinical characteristics of patients suffering from autoimmune hemolytic anemia(AIHA) associated with acute kidney injury (AKI).Methods The clinical data of 2 case patients with AIHA and AKI in the People's Hospital of Huadu District of Guangzhou was analyzed and associated literatures were reviewed.Results Two patients presented with severe anemia,raised reticulocyte count,positive Coomb's test,and acute renal failure.They were no effect therapy when treatment with agent of erythropoietin (EPO),iron or folic acid.The level of serum indirect bilirubin was at normal range.Liver function and serum LDH were normal too.However,treatment with corticosteroids was effective.Conclusion When patients suffering AKI occur worsening anemia,AIHA probably should be concerned.Treatment with corticosteroids early is effective.

Objective To evaluate the clinical value of prenatal ultrasound in the diagnosis of congenital anal atresia.Methods A retrospective analysis was studied from January 2013 to December 2015 which analyzed abnormal sonographic features by prenatal ultrasound screening in 13 cases of anal atresia.Results 13 cases of anal atresia were confirmed after abortion,miscarriage or birth which were not probed target ring signby prenatal ultrasound screening.That included 2 simple atresia cases and 11 cases associated with other malformations and abnormalities including 8 cases with bowel dilatation and 3 cases with intestine visible echogenic meconium. Conclusion Prenatal ultrasound screening for fetal congenital anal atresia can be detected.But for lacking of specific diagnostic standard,the detection rate of anal atresia remains very low.

Objective To evaluate the reproducibility and agreement in the measurement of liver volume with multi-slice spiral computed tomography(CT).Methods Spiral CT was performed in 20 cases(14 healthy volunteers and 6 patients with cirrhosis).Images were transferred to a workstation,and volumes were measured by three observers with 5-year abdominal CT experience.Each observer performed the measurements in all subjects twice.Intra-observer and inter-observer variability was calculated using analysis of variance of the repeatedly measured results.The agreement was analyzed with the intra-class correlation coefficient(ICC).Reliability analysis in inter-observer was performed with Cronbach's alpha coefficient.Results Data measured by Observer A were(1 265.53?421.43)cm3 and(1 272.30?414.73)cm3 [difference:(0.80?2.20)%,P=0.15,ICC=0.998 8],Observer B(1 299.97?403.48)cm3 and(1 310.23?404.04)cm3 [difference:(0.90?1.90)%,P=0.07,ICC=0.998 3],Observer C(1 282.18?425.91)cm3 and(1 289.73?424.66)cm3 [difference:(0.70?2.60)%,P=0.19,ICC=0.998 3],respectively.The mean liver volumes by the three observers were(1 268.91?418.07)cm3,(1 305.10?403.59)cm3,and(1 285.97?425.10)cm3,respectively(P=0.07,ICC=0.986 9,Cronbach's alpha coefficient=0.995 6).Conclusion High reproducibility and agreement in the measurement of liver volume can be achieved with multi-slice spiral CT.

Objective To overcome the disadvantages of the traditional auscultating method and design the electro-auscultating visual system integrating the monitoring and recording functions.Methods The portable system consisted of the module pre-gathering and electro-auscultating heart-sound signal,the circuit of A/D converting and MCU,as well as the device of signal LCD-displaying in real-time and the USB mass storage device.Results Portable electro-auscultating system was realized,which integrated the functions of watching,monitoring and recording.Conclusion The portable system is more convenient for clinical doctors to get correct information from patients,benefiting to the clinical pathology-statistics and profound development of the related data.

Objective:To summarize and analyze the disease characteristics of the patients in Chinese Level-II Hospital for UN Peacekeepers in Liberia,for offering some references to the pre-mission training of the successive contingents as well as the improvement of medical support to the peacekeeping mission.Methods:A statistical analysis was made of the case history of all the outpatients and inpatients received in Chinese Level-Ⅱ Hospital for UN Peacekeepers in Liberia from May 2004 to December 2005. Results:Out of the total of 7107 outpatients,2539(35.7%) were medical cases,1205(17.0%) surgical,1136(16.0%) dental,787(11.1%) dermatological and 763(10.7%) contagious cases.Malaria was the main type of contagious disease,which accounted for 86.1% of all the contagious cases.There were 39 cases of AIDS/HIV(acquired immunodeficiency syndrome / human immunodeficiency virus).Of the 453 inpatients,233(51.4%) were contagious and 166(36.7%) medical cases,and 185(40.8%) of the total number suffered from malaria.The average hospital stay was 5.2 days.Conclusion:Adequate drugs and equipments should be prepared for both common and special diseases in the peacekeeping mission area.During the pre-mission training at home,special emphasis should be laid on the prevention and treatment of common diseases and frequently occurring diseases,particularly on the diagnosis of and protection against AIDS.

This paper proposes a one-way micro valve with a simple structure and a simulation design for the engineering capsule. We have now got its design parameter selection method and its mechanic characteristic from experiments.
Capsules ; Computer Simulation ; Drug Delivery Systems ; methods ; Equipment Design ; Infusion Pumps, Implantable ; Technology, Pharmaceutical ; instrumentation ; methods

Objective To stuay CT findings of adrenal gland trauma(AGT).Methods The CT images of adrenal gland trauma which were found in 1712 patients who had emergency abdominal CT examinations for assessment of blunt force trauma were analyzed restrospectively.Results Among 1712 cases.we identified 29 patients(1.7%)with adrenal gland traumatic lesions.A total of 32 lesions were found.Twenty-six lesions were unilateral(23 right-sided,3 left-sided),and 3 cases were bilateral(6 lesions).Main CT findings were as followed:(1)Adrenal hematomas appeared as discrete round or oval masses expanding the adrenal gland in 22 patients with 25 lesions.The lesions were hyperdense or of homogeneous density in plain scan and did not enhance in three-phase contrast-enhanced dynamic scan.The splayed adrenal limbs around hematoma were enhanced significantly as eurvilinear structures.(2)Mild to moderate uniform swelling of gland was shown in 4 cases.(3)Diffuse irregular hemorrhage obliterating the gland was shown in 3 cases.Contrast extravasation was shown in 1 case,which was active bleeding from the broken adrenal gland proven by operation.Associated CT findings included strand-like hemorrhage of the periadrenal fat and posterior pararenal hemorrhage mimicking thickened diaphragmatic crus.The concurrent injuries of ipsilateral thorax or/and abdomen were found in 25 patients.Conclusion The AGT has typical CT findings.Familiarity with characteristic CT findings of adrenal trauma is essential for the radiologist to avoid misdiagnosis.

Objective To study the effect of transfection of antisense MBD1 gene eukaryotic expression vector on the expression of MBD1 gene in human cholangiocarcinoma cell line QBC-939.Methods The(constructed) antisense MBD1 gene eukaryotic expression vector was transfected into the human(cholangiocarcinoma) cell line QBC-939 using lipofectamine transfection reagents,and positive cell clones were obtained using G418 selection after transfection.The constructed recombinant vector was transfected into(QBC-939) cells successfully and was confirmed by amplifying the exogenous neo~R gene with PCR method.The expression level of MBD1 gene mRNA and protein was detected by RT-PCR and FCM methods respectively.Results Following the transfection,the MBD1 gene mRNA level in human cholangiocarcinoma cell line QBC-939 decreased from 0.912?0.022 to 0.215?0.017,and the MBD1 gene protein level also(decreased) from(80.19?5.05)% to(35.11?4.05)%.There were very significant differences on the expression both at the transcription and post-transcription levels of MBD1 gene between non-tranfection group and the antisense MBD1 gene eukaryotic expression vector transfection group(P

Fed with high-fat diet and assessed by hyperinsulinemia-euglycemia clamp technique, rat models with insulin resistance were successfully induced. Compared with normal chow group ( NC ), serum concentrations of free-fatty acids(FFAs) and baseline insulin in high-fat diet group(HF) was higher( P＜0.05 ), the average glucose infusion rate from 60 to 120 min( GIR60-120 ) was lower( P＜0.01 ), and the expression of p-IRE-lα in the liver was higher( P＜0.05 ). Furthermore, the expression of p-IRE-1α in the liver was positvely correlated with the serum concentration of FFAs. All these data indicate that high-fat diet may induce endoplasmic reticulum stress in the liver by elevating serum concentration of FFAs, and may participate in the genesis of insulin resistance via p-IRE-1α.

Objective To explore the follow-up value of phonocardiogram D/S ratio in CHF patients with treatment.Methods Forty-eight CHF patients who had never under-went regular treatment were collected in our hospital,given the regular anti-heart failure treatment for 3 months.NYHA heart failure classification,resting heart rate(RHR),and D/S ratio of phonocardiogram were recorded,six-minutes walk distance(6MWD),Left ventricular end diastolic diameter(LVEDd),the left ventricular ejection(LVEF) were tested before and after treatment.After 3-month treatment,the resting heart rate 65 times as the watershed,the patients were classified into two groups:one was up to standard,and the other was not up to standard.D/S ratio,RHR,6MWD,LVEDD,LVEF and the improvement of the heart function were compared between the two group.Results Exception the resting heart rate,the in-dicators had no significant improvement in the group of not up to standard compared with the before treatment,and D/S ratio, 6MWD,LVEF was improved significantly after three months treatment(P <0.05).In the group of up to standard,the D/S ratio was improved significantly after one months treatment(P <0.05),while after three months treatment all indicators were improved significantly(P <0.05).Comparison between the two groups,the RHR,D/S ratio were significantly improved(P <0.05 ),LVEF was significantly improved after three months treatment(P <0.05).At the same time,the D/S ratio,6MWD and NYHA classifica-tion of cardiac function was significant negatively correlated(P <0.05).Conclusion The D/S ratio could reflect heart functional im-provements in patients with CHF after anti-heart failure treatment,and it could reflect the heart functional improvement earlier and more significant in the group up to standard,it has a good clinic follow-up value in CHF patients with anti-heart failure treatment.