1.Expression of Endothelin in Kidneys of Rats with Unilateral Ureteral Obstruction
guo-lan, XING ; zhang-suo, LIU ; jian-guo, WEN
Journal of Applied Clinical Pediatrics 2004;0(09):-
Objective To determine the expression of endothelin in kidneys of rats with unilateral ureteral obstruction (UUO). Methods Rats were evenly divided into two groups. Some were subjected to complete ligation of left ureter and the others with sham operation were used as control group.Four weeks later, we examined the mRNA expressions of ET-1 in kidneys of all these rats using reverse transcription-polymerase chain reaction (RT-PCR) method.The immunohistochemistry analysis was carried out to investigate the protein level of ET-1. The urinary concentration of ET-1 was determined by radioimmunoassay.Results In UUO group, the urinary concentration of ET-1 increased substantially;both gene expression and protein level of ET-1 were much higher than those in control group. At the same time, tubulointerstitial fibrosis was present in the kidneys of rats with UUO. Conclusion ET-1 is involved in the pathogenesis of the injury of UUO.
2.Methodology of DNA staining by Azure A.
Jian-yun ZHOU ; Song HE ; Lan YU ; Yan GUO
Chinese Journal of Pathology 2006;35(9):568-569
Azure Stains
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chemistry
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DNA, Neoplasm
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analysis
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chemistry
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Humans
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Rosaniline Dyes
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chemistry
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Staining and Labeling
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economics
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methods
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Stomach Neoplasms
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genetics
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pathology
4.Protective Effects of Bosentan on Kidney in Rats with Unilateral Ureteral Obstruction
guo-lan, XING ; zhang-suo, LIU ; jian-gua, WEN
Journal of Applied Clinical Pediatrics 2003;0(10):-
Objective To research the effects of bosentan in on unilateral ureteral obstruction (UUO) rats. Methods Wistar rats were randomly divided into three groups. UUO group was subjected to complete ligation of left ureter, sham operation group with sham operation was used as control. Bosenten group was subjected to complete ligation of left ureter and to stomach with bosentan suspension. Three groups were killed at 5,10,15,20 d. We examined renal pathological changes and the expressions of transforming growth factor-?1 (TGF-?1) in kidney of all rats using immunohistochemistry analisis method. The plasma and ureteral obstructive kidney urinary concentration of endothelin - 1 (ET - 1) was determined by radioimmunoassay. Results In Bos and UUO groups, the urinary concentration of ET - 1 increased substantially and the expression of TGF - ?1 were much higher than those in the S group. At the same time.tubulointerstitial fibrosis was present in the kidney of rats in Bos and UUO group. But the expression of TGF- ?1 and tubu-lointerstitial fibrosis in Bos group were lower than those of UUO group. Conclusion Bosentan can effectively amelirate tubulointerstitial fibrosis in rats with UUO by ET and TGF - ?1 machanism.
5.Cyclodextrin and Its Derivatives as Non-viral Gene Vectors
Xiao ZHANG ; Zhi-Zhang GUO ; Lan HUANG ; Jian-Shu LI ;
China Biotechnology 2006;0(10):-
Cyclodextrin(CD) is gradually applied in the nonviral gene vector system,due to its biocompatibility and flexibility of tailing via structural modification,polymerization or supramolecular combination.The ideas and research progress of the CD,its low molecular derivatives,CD polymers and CD supramolecular combination in the field of norviral gene vectros were reviewed,and their "structure-safety-transfection efficiency" relationships were discussed.
6.Determination of phosphorus oxychloride in the air of workplace by ion chromatography.
Shu-lan ZHAO ; Jian ZHANG ; Wan-chao ZHANG ; Jian-guo LI ; Hao ZHANG ; Hao LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(2):148-149
OBJECTIVETo establish a ion chromatography method for determination of phosphorus oxychloride in the air of workplace.
METHODThe phosphorus oxychloride in the air of workplace was collected by absorb liquid and turned into hydrochloric acid, then separated in column and detected with conductivity detector, qualified by elution time and quantified by peak height or peak area.
RESULTSThe linear range of phosphorus oxychloride in air of workplace was 0.72 ∼ 5.76 µg/ml with its correlation coefficient 0.9999. The detecting limit of the method was 0.12 µg/ml. The smallest detecting concentration of the method was 0.08 mg/m(3) for 15 L sampling air. Relative standard deviation was 3.3% ∼ 6.2% and the recovery was 97.8% ∼ 103.8%. The sample could be resaved at room temperature at least for seven days.
CONCLUSIONThe indicators of the method correspond GBZ/T 210.4-2008«Guide for establishing occupational health standards-Part 4: Determination methods of air chemicals in workplace». It is a good method to determine phosphorus oxychloride in the air of workplace.
Air Pollutants, Occupational ; analysis ; Chromatography, Gas ; methods ; Ions ; analysis ; Phosphorus Compounds ; analysis ; Workplace
7.Effect of different local anesthetics on analgesia after harvesting grafts from the scalp in burn patients
Guihong ZHU ; Bin YI ; Qiao GUO ; Ying LAN ; Hongchun WANG ; Jian CHEN ; Xiaolu LI ; Jian CUI
Journal of Regional Anatomy and Operative Surgery 2014;(6):630-631,632
Objective To investigate the different effect among ropivacaine,bupivacaine,lidocaine on analgesia after harvesting grafts from the scalp in burn patients. Methods 84 patients who need harvesting grafts from the scalp after burn were divided in 4 groups random-ly(n=21). Patients in group C hypodermically injected with saline 200 mL were control,while patients in group R injected with 0. 05% ropi-vacaine 200 mL,group B with 0. 188% bupivacaine,and group L with 0. 1% lidocaine. Motor activity assessment scale( MAAS) and visual analogue scale(VAS) were made before anesthesia(T0) and 20 min,5 h,10 h after awake of patients. VAS were made focus on head and body in part. Vital signs were also monitored and recorded for assessment of security. Results All patients in 4 groups had passed the period of operation safely. Patients in group R have better VAS than other groups. Conclusion Low concentration ropivacaine hypodermically injec-tion of head is helpful to relieve the pain after harvesting grafts from the scalp.
8.Effect of transcutaneous electrical acupoint stimulation on gastrointestinal function in perioperative period of laparoscopic intestinal surgery
Lan YUAN ; Jun GUO ; Wei TANG ; Jian WANG ; Guoqiang FU ; Feng GUO
The Journal of Clinical Anesthesiology 2017;33(6):567-569
Objective To observe the effect of transcutaneous electrical acupoint stimulation (TEAS) on the recovery of gastrointestinal function after laparoscopic intestinal operation.Methods Sixty patients of the selective laparoscopic intestinal resection, 28 males and 32 females, aged 18-65 years, ASA physical status Ⅰ or Ⅱ, were enrolled and randomly allocated into two groups: TEAS group and control group, 30 in each group.Patients in TEAS group accepted transcutaneous electrical acupoint stimulation treatment at Neiguan, Hegu, Zusanli points from the time before induction of anesthesia to 3 days after surgery, and patients in the control group were treated with transcutaneous electrical acupoint stimulation, but the electrode pads were just attached on the related points with no electric stimulation.Plasma motilin concentrations preoperatively, postoperatively 12, 24, 48 and 72 h were measured in the two groups.The recovery time of intestinal peristalsis, anal flatus time, in-hospital time and the incidence of nausea and vomiting within 3 days after operation were observed.Results Compared with the control group, serum motilin concentration postoperative 24 h increased significantly [(218.5±52.3) pg/ml vs (141.8±45.8) pg/ml, P<0.05], the time of intestinal peristalsis recovery [(19.4±3.2) h vs (29.6±7.8) h, P<0.05] and flatus [(23.2±4.7) h vs (36.5±8.9) h, P<0.05] were shorter, the incidence of postoperative nausea and vomiting within 3 days after operation decreased significantly in TEAS group (16.7% vs 36.7%, P<0.05).There was no statistically significant difference of the in-hospital time between the two groups.Conclusion TEAS can promote the recovery of gastrointestinal function in patients undergoing laparoscopic intestinal surgery.
9.A simulation design of a one-way micro valve for the micro engineering capsule.
Lan LUO ; Xiao-lin ZHENG ; Wen-sheng HOU ; Jian-guo CUI
Chinese Journal of Medical Instrumentation 2005;29(4):241-243
This paper proposes a one-way micro valve with a simple structure and a simulation design for the engineering capsule. We have now got its design parameter selection method and its mechanic characteristic from experiments.
Capsules
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Computer Simulation
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Drug Delivery Systems
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methods
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Equipment Design
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Infusion Pumps, Implantable
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Technology, Pharmaceutical
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instrumentation
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methods
10.Antiproliferation and induction differentiation of rosiglitazone in human gastric carcinoma transplanted into nude mice
Fang-Zhi CHEN ; Li-Hui ZHU ; Jian-Feng HU ; Li ZHANG ; Guo-Qing LI ; Lan WEN
Cancer Research and Clinic 2000;0(06):-
Objective To investigate the antiproliferation and induction differentiation of human gas- tric carcinoma which human gastric lower-differentiation mucinous carcinoma MGC-803 cells transplanted in- to nude mice by using rosiglitazone(ROS),and to preliminarily explore the mechanism of differentiation. Methods The mice were randomly divided into five groups:model,ATRA,ROS 25 mg?kg~(-1),ROS 50 mg/kg, ROS 100 mg/kg.After that the volumes were measured and inhibition rates were calculated.The cell cycle was detected by FCM.The protein expression level of Mucin SAC was detected by immunohistochemistry. Results The volume of tumor decreased significantly in ROS treatment groups,the differences had statistical significance compared with model group(P0.05).The xenograft tumors of ROS groups demonstrated the characteristics of differentiation.Xenograft tumor cells were arrested in G_0/G_1 phase,and the cells in S phase decreased significantly,and up-regulated Mucin SAC gene expression.Conclusion ROS could inhibit the growth of tumor,and the effect were dose-dependent with ROS.ROS could induce the differentiation of Xenograft tumor cells of gastric cancer.Its mechanism might be related to the inhibit of transition from G_1 to S phase,degrade the activity of proliferation,regulate the expres- sion of Mucin 5AC.