3.Analysis on The risk factors of diabetic nephropathy in elderly type 2 diabetes mellitus
Jian FENG ; Xinggang DONG ; Zhiman YU
Chinese Journal of Postgraduates of Medicine 2010;33(16):21-23
Objective To investigate the correlation risk factors of diabedc nephropathy(DN)in elderly type 2 diabetes mellitus(T2DM).Methods Eighty-six cases of elderly T2DM were divided into two groups,DN group(43 cases)and NDN group(43 cases).Their age,fasting plasma glucose(FPG),2 hour postprandial glucose(2hPG),triglycerides(TG),total cholesterol(TC),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol(LDL-C),hemoglobin A1c(HbA1c),systolic blood pressure (SBP),diastolic blood pressure(DBP)were observed.Results Compared with that in NDN group,2hPG,TG,SBP,HbA1c wag obviously increased in DN group[(14.13±4.46)mmol/L vs(11.19 ±4.22)mmol/L,(1.51±0.79)mmol/L vs(1.20 ±0.53)mmol/L,(141.16±19.08)mm Hg(1 mm Hg=0.133 kPa)vs(132.79 ±17.40)mn Hg,(7.55±2.09)%vs(6.65±2.02)%](P<0.01 or<0.05).Multiple regression analysis showed that the risk factors of DN included 2hPG and TG.Conclusion DN in T2DM is related with 2hPG and TG.
5.Investigation of Skeletal Age of Adolescent in Yantai Area
Chinese Journal of Rehabilitation Theory and Practice 2006;12(9):804-805
ObjectiveTo assesses the skeletal development of adolescent in Yantai area, and investigates the characteristics of the tendency of their skeletal development. MethodsTotally 1180 healthy adolescents ( 574 males and 606 females ) at 10 to 20 years of age living in Yantai area were randomly selected, and their left wrist were radiated at the posterior-anterior position. The bones at wrist were scored according to the Chinese Wrist Skeletal Development Standard CHN Method. The correlation among skeletal age, chronological age and the sex were analyzed. ResultsSkeletal age positively correlated with chronological age (male: r=0.943,females: r=0.942,totally: r=0.942). ConclusionThe skeletal development of adolescent in Yantai area has the characteristic of acceleration. So it is necessary to establish a new skeletal age standard for the skeletal development of adolescent.
6.Evaluation the Effect of Dexamethasone Addition in Anesthesia on Postoperative Quality of Life after Extraction of Impacted Wisdom Teeth
Feng QIAO ; Hengxiang MA ; Rui DONG ; Jian ZHANG
Tianjin Medical Journal 2014;(12):1240-1242
Objective To investigate the effect of dexamethasone addition in block anesthesia before surgery on postoperative quality of life after extraction of impacted mandibular wisdom teeth. Methods Using a random number table, 120 cases of patients were divided into experimental and control groups, each group contains 60 cases whose mandibular wis?dom tooth will be extracted. Before the surgery, the experimental group patients perform local block anesthetic injection with 3 mL out of 5 mL mixture of 2%lidocaine 4 mL+dexamethasone 1 mL (5 mg) to block nerve. The control group was given block anesthesia of inferior alveolar nerve with 3 mL of 2%lidocaine block anesthesia alone. Improved postoperative symp?toms severity scale (PoSSe) was used to evaluate patients’quality of life after tooth extraction comprehensively and quantita?tively by analysis and comparison of the two sets of scores. Results The experimental group’s PoSSe scores were 18.059 1 ± 13.210 9, which was lower compared with control group (27.819 3 ± 15.716 9). In experimental group, diet, language, swelling, pain and daily points were lower than the control group (P<0.05). Conclusion Topical application of dexamethasone in mandibular wisdom tooth extraction reduces postoperative adverse reactions in a certain degree and im?prove postoperative quality of life.
7.Clinical efficacy of intermittent androgen suppression treatment of advanced prostate cancer in elderly patients
Chun YANG ; Jun FENG ; Jian DONG ; Deshui YU ; Jun CONG
Chinese Journal of Geriatrics 2014;33(9):980-982
Objective To explore clinical effect and safety of intermittent androgen suppression treatment of advanced prostate cancer in elderly patients.Methods 78 patients with advanced prostate cancer were enrolled,and randomly divided into the observation group and the control group (n=39 each).Patients in observation group were treated with intermittent androgen suppression treatment.Patients in control group were treated with persistent androgen suppression treatment.Results The time of therapy was much longer in observation group than in control group (P< 0.001).Quality of life was better in observation group than in control group (P<0.05).The incidences of adverse reactions including anemia,fever and abnormal liver function were lower in observation group than in control group (all P<0.05).Conclusions Intermittent androgen suppression treatment prolongs time to androgen-independent progression,improve the quality of life,reduce drug dosage and the incidence of adverse reactions in the treatment of advanced prostate cancer in the elderly.
8.THE EFFECTS OF PROPANOIC ACID ACCUMULATION ON THE GROWTH AND ACID-PRODUCTION OF PROPIONIBACTERIUM SHERMANII
Hong YI ; Lili WANG ; Huiyong FENG ; Xiuping DONG ; Jian HOU ;
Microbiology 1992;0(03):-
The effects of propanoic acid accumulation on the growth and acid production of Propionibacterium shermanii are reported in this paper, the glucose consumption, acid production and cell growth curve are measured in the batch fermentation process with 6%glucose serves as carbon source and pH value controlled at 6 5 If 1%, 3%, and 6% propanoic acid are added to the broth 24 hours after inoculation, the cell dry weights obtained at the end of the fermentation change to 75 3%, 65 4%, 52 9% of the CK respectively, at the same time, the acid accumulation becomes 79 3%, 69 2%, 39 3% of the CK respectively But even 6% propanoic acid is added, the glucose consumption and acid production could not be stopped completely The cell dry weight increased 60% if part of the propanoic acid is removed from the broth and replaced by fresh medium
9.Clinical manifestation and gene mutation analysis of VHL syndrome families with first diagnosis of cerebral hemangioblastoma
Yuhao ZHOU ; Jian LIU ; Minghao DONG ; Luqian FENG ; Liangzhao CHU
Chinese Journal of Behavioral Medicine and Brain Science 2017;26(6):560-563
Objective To detect the mutations of Von Hippel-Lindau (VHL) gene via analyzing the prevalence of family members of VHL syndrome,clinical diagnosis and treatment,and gene analysis of patients with hemangioblastoma.methods All members of the VHL syndrome family members improved all relevant tests and plotted the family map.5 ml peripheral blood was extracted for gene sequencing,and the sequencing Result s were compared with the reported mutations of VHL gene in NCBI database.Result s(1)Analysis of clinical data of four members of the family:Ⅰ-2,Ⅱ-1,Ⅱ-5 suffering from central nervous system hemangioblastoma, Ⅱ-3 with pancreatic,retinopathy and pheochromocytoma,and Ⅱ-5 also combined with kidney,pancreatic lesions.The second generation of patients in the family have been treated surgically.(2)Gene sequencing Result s showed that all subjects in the test had the same mutation:exon2 109 sequence ATATCACACTGCCA was deleted and termination codon UGA appeared in exon 502.Conclusion Through the mutations of the VHL syndrome family,it is found that the family mutation type is a new mutation.For patients with central nervous system hemangioblastoma-based should be suspected of the disease and improve the family history survey.Once the diagnosis of familial VHL syndrome patients are confirmed,it is necessary to inform the other members of the family for clinical screening,and carry out genetic testing to reduce the harm of the disease to the greatest extent.
10.Construction of a siRNA expression plasmid targeting CXCR4 and its effect on the invasion capability of the breast cancer ceils in vitro
Junfei FENG ; Jian DONG ; Min HONG ; Change GAO
Basic & Clinical Medicine 2009;29(12):1286-1290
Objective To construct and identify the siRNA eukaryotic expression vector targeting gene CXC chemo-kine receptor-4 and explore its role in invasion process of breast cancer cells in vitro. Methods Two siRNAs were designed and synthesized according to the coding sequence of CXCR4 gene and cloned into eukaryotic expression plasmid pGE-1-U6/kna. The constructed CXCR4-siRNA expression vector was transfected into MDA-MB-231 cells by liposome. Western blot was used to evaluate the suppression of CXCR4 expression in different groups. The inva-sion and migration of MDA-MB-231 cells were evaluated by cell invasion assay in vitro. Results Enzyme digestion and DNA sequencing confirmed that the CXCR4-siRNA expression vector was successfully constructed. After trans-fection,the CXCR4-siRNA obviously suppressed the expression of CXCR4 compared with control groups and the ability of cell migration was decreased markedly. Conclusion CXCR4-siRNA expression vector can effectively sup-press CXCR4 expression in the breast cancer cells and decrease potential of cell invasion, which may provide a no-vel strategy for gene therapy of breast cancer metastasis.