1.Detection of Interleukin-4,-6,-8 in Children with Idiopathic Thronbocytopenic Purpura
shu-ren, ZHANG ; jian-fei, FENG ; guang-yao, SHENG
Journal of Applied Clinical Pediatrics 1986;0(01):-
Objective To study the changes of interleukin-4(IL-4),IL-6,IL-8 in children with idiopathic thrombocytopenic purpura(ITP).Methods The serum of IL-4,IL-6,IL-8 in 35 ITP patients and 20 normal control children were detected by enzyme-linked immunosorbent assay.Results The serum IL-4,IL-6,IL-8 in ITP patients were elevated. There were significant difference between ITP group and control group(P
2.Evolution of U.S.mobile medical care units on battlefield from mobile army surgical hospitals to forward surgical teams
Jian YANG ; Fei PAN ; Zhan SHU ; Tanshi LI ; Feng TIAN
Military Medical Sciences 2016;(1):67-69
Medical care on the battlefield is the core and basis of echelons of care.This review summarizes the background and characteristics of medical care units on the battlefield from the birth and growth of mobile army surgical hospitals before being replaced by forward surgical teams and combat support hospitals, since the United States Armed Forces began to lead the world military revolution during and after the World WarⅡ.Quick adaptation to the combat envi-ronment and the combat modes is the main reason that medical care units on the battlefield are adjusted continuously.This paper may provide some ideas for the development of our medical care units on the battlefield in the future.
3.Experimental study of Bushen Decoction on the protection of the endothelial cell in post-menopause atherosclerosis rabbits
Zhenyu FEI ; Wenjian WANG ; Weihua CHEN ; Jian YING ; Feng TANG ;
Chinese Traditional Patent Medicine 1992;0(06):-
Objective:The effect of “BU SHEN Decoction” on serum lipid, nitric oxide (NO), endothelin (ET), and micro structure of the endothelial cell was observed in experimental post menopause atherosclerosis rabbits.Methods: The post menopause atherosclerosis rabbit model was established by feeding high cholesterol diet after the rabbits had been given the castration operation and injected with bovine serum albumin. After “BU SHEN Decoction” had been given for 12 weeks, the blood sample was collected. Results: “BU SHEN Decoction” can decrease the serum cholesterol (CHO), triglyceride (TG)( P
4.Calcium-dependent modulatory effect of norepinephrine on the Ia antigen expression of the macrophage
Jian-Jun HUANG ; Fei-Li GONG ; Xin-Wei FENG ;
Chinese Journal of Immunology 1985;0(06):-
Fura—2 was used as a Ca~(2+) indicator to determine the intracellular calcium ion concentra-tion(〔Ca~(2+)〕i)of rat peritoneal macrophages(RPM?s),and APAAP enzyme immnoassay was ap-plied to detect the expression of Ia antigens on RPM?s.The results showed that norepinephrine(NE,10~(-9)mol/L)could markedly increase the〔Ca~(2+)〕i of the RPM?s(p
5.Characteristics and treatments of spinal fractures associated with dural tears
Fei LEI ; Daxiong FENG ; Jianping KANG ; Fei YE ; Yunlong ZHOU ; Qingzhong ZHOU ; Jian YANG
Chinese Journal of Trauma 2016;32(10):929-933
Objective To investigate the characteristics and treatment effects in patients with spinal fractures associated with dural tears.Methods A retrospective analysis was made on 185 patients with spinal fractures presenting to hospital from February 2013 to February 2015.There were 103 males and 82 females,aged 17-73 years (mean,58 years).Causes of injury were high falls in 72 patients,traffic collisions in 58,hitting by heavy objects in 41,ground-level falls in 12,and collision events in two.Cervical spine fractures were seen in 65 patients,thoracic vertebra fractures in 51,and lumbosacral vertebral fractures 69.Neurologic deficit was assessed using the American Spinal Injury Association (ASIA) score,including grade A in 24 patients,grade B in 22,grade C in 26,grade D in 37 and grade E in 76.Eighty patients were managed by simply anterior surgery,97 by posterior surgery,and eight by anterior-posterior surgery.Twenty-one patients were found with dural tears (group A) and 164 patients without dural tears (group B).Incidence of dural tears in cervical,thoracic and lumbosacral vertebral fractures were recorded and compared.Preoperative neurologic deficit,laminar fracture and spinal canal encroachment rate were compared between groups.Neurological function and complications associated with dural repair were detected.Results In group A,ten patients were rated ASIA grade A,five grade B,three grade C,one grade D and two grade E.In group B,14 patients were rated ASIA grade A,17 grade B,23 grade C,36 grade D and 74 grade E.Group A accounted for 11% (7/65) of cervical,10% (5/51) of thoracic,and 13% (9/69) of lumbosacral spine fractures (P > 0.05).Nineteen patients (91%) in group A were complicated with neurological deficit,compared to ninety patients (54.9%) in group B (P < 0.01).Eighteen patients (86%) in group A had laminar fractures,compared to fifteen patients (9.1%) in group B (P < 0.01).In group A,rate of spinal canal encroachment was (62.3 ± 12.1)% and 17 patients (81%) showed spinal canal encroachment of greater than 50%.While in group B,rate of spinal canal encroachment was (36.2 ± 15.6)% and 25 patients (15.2%) showed spinal canal encroachment of greater than 50% (P < 0.01).For dural tears in group A,11 patients were treated by direct suturing,four by thoracolumbar fascia repair,three by artificial dural coverage and three by fibrin glue sealing.In group A,19 patients were followed up and one of them presented persistent cerebral spinal fluid leak that necessitated an irrigation and debridement to cure.ASIA score was improved from grade A to B in two patients,grade B to C in one,grade C to D in one and grade D to E in one at the final follow-up.Conclusions Majority patients with spinal fractures associated with dural tears exist severe neurologic deficit,spinal canal encroachment and laminar fractures.Incidence of dural tear in cervical,thoracic and lumbosacral vertebral fractures is similar.Incidence of complications related to dural tear repair is low,but the neurological function recovery is poor after operation.
6.Primary testicular yolk sac tumor: clinicopathological study of 8 cases.
Fei-Fei LIU ; Jian-Jun WANG ; Qin SHEN ; Bo YU ; Zhen-Feng LU ; Heng-Hui MA ; Qun-Li SHI
National Journal of Andrology 2014;20(5):435-438
OBJECTIVETo investigate the clinicopathological characteristics, diagnosis and treatment of primary testicular yolk sac tumor (YST).
METHODSWe studied 8 cases of primary testicular YST by microscopy and immunohistochemistry.
RESULTSThe 8 cases of primary testicular YST, including 2 consultation cases, were confirmed from 1998 to 2013, accounting for 10.7% (8/75) of all the testicular germ cell tumors diagnosed in our hospital. The patients ranged in age from 7 to 43 years, 23.9 years on average. The main clinical manifestation of the patients was painless unilateral testis swelling. Microscopically, reticular tissues, schiller-duvaI (S-D) bodies, and eosin-stain transparent bodies were seen in the tumors. One of the cases was confirmed to be simple YST, while the other 7 mixed YST. AFP was a characteristic immunophenotype marker of the tumors.
CONCLUSIONPrimary testicular YST is a rare malignancyr with poor prognosis. Its diagnosis depends on preoperative AFP test and postoperative pathology. Comprehensive treatment, including orchiectomy, chemotherapy, and radiotherapy, can prolong the survival of the patients.
Adolescent ; Adult ; Child ; Endodermal Sinus Tumor ; metabolism ; pathology ; therapy ; Humans ; Immunohistochemistry ; Male ; Neoplasms, Germ Cell and Embryonal ; metabolism ; pathology ; therapy ; Orchiectomy ; Rare Diseases ; metabolism ; pathology ; therapy ; Testicular Neoplasms ; metabolism ; pathology ; therapy ; Young Adult
7.Expressions of mir-132, mir-134 in the different regions of rat brain after sleep deprivation
Han RONG ; Tiebang LIU ; Haichen YANG ; Fei FENG ; Dan XU ; Jingjing LIU ; Jian ZHANG ; Qijie SHEN
Chinese Journal of Behavioral Medicine and Brain Science 2012;21(6):515-517
ObjectiveTo investigate the effects of sleep deprivation on expressions of Mir-132,mir-134 in the different regions of rat brain.MethodsAll the male SD rats were divided into control group ( normal sleep group),sleep deprivation (SD).The modified multiple platform method (MMPM) was used to establish sleep deprivation model.Mir-132,mir-134 level was detected by real time PCR.ResultsMir-132 were significantly increased in SD groups in hippocampus compared with the control groups ( 51.87 ± 8.13 vs 67.25 ± 7.59 ) (P <0.01 ).Mir-134 were significantly decreased in SD groups compared with the control groups( 1.82 ±0.15 vs 1.45± 0.12 )(P < 0.01 ).There were no statistically significant differences in cortex and thalamus (P > 0.05 ).Cortex mir-132 level in SD group and control group was 1.57 ±0.10,1.48 ±0.11 respectively,and it was 1.37 ±0.09,1.36 ±0.11 in thalamus;Cortex mir-134 level in SD group and control group was 98.26 ± 5.17,100.80 ±4.15respectively,and it was 97.56 ± 6.28,91.01 ± 4.07 in thalamus.ConclusionThe upregulation of mir-132 and downregulation of mir-134 implies that two miRNAs did opposite actions in the processes of sleep deprivation.This findings indicate that hippocampus mir-132,mir-134 levels in the SD rat may reflect associated depressive patho-physiological processes.
8.Significance of Chronic Aplastic Anemia in Children by Determining Soluble Stem Cell Factor and Its Receptor
xiao-hui, MENG ; jun, WANG ; shu-yun, YUAN ; jian-fei, FENG ; hong, WANG
Journal of Applied Clinical Pediatrics 2006;0(15):-
Objective To investigate the function of soluble stem cell factor receptor(s-kit)and stem cell factor(SCF) in chronic aplastic anemia(CAA).Methods ELISA assay was employed to determine the levels of s-kit and SCF in peripheral blood of CAA patients and umbilical cord blood.Results The levels of s-kit and SCF in peripheral blood of CAA patients are lower than those of normal group and umbilical cord blood group.Conclusions The decreased levels of s-kit and SCF show that s-kit and SCF may play a role in CAA mechanism.The raised levels of s-kit and SCF show that s-kit and SCF may be applied in the field of cord blood transplantation.
9.Study of an animal model of post-menopausal atherosclerosis in rabbit and observation of the effect of herbal formula of tonifying-kidney on it
Wenjian WANG ; Zhenyu FEI ; Xiaodong FU ; Weihua CHEN ; Jian YING ; Feng TANG
Journal of Integrative Medicine 2003;1(2):113-5, 131, 154
OBJECTIVE: To establish an animal model imitating post-menopausal atherosclerosis in rabbit and observe the effect of the herbal formula of tonifying-kidney on this animal model. METHODS: The New Zealand rabbits were treated with ovariectomy, high cholesterol diet plus immune injury, and both of ovariectomy and high cholesterol diet plus immune injury respectively. The blood lipid level and the intima and media of the aorta were observed. RESULTS: The rabbits treated with ovariectomy and high cholesterol diet plus immune injury changed markedly and had low level of estrogen, high levels of CHOL, TG and dysfunction of the arterial endothelium cells. however, the chinese herbal formula of tonifying-kidney could relieve these changes. CONCLUSION: The New Zealand rabbits treated with both of ovariectomy and high cholesterol diet plus immune injury could result in the changes imitating post-menopausal atherosclerosis, and it could be improved by the treatment of herbal formula of tonifying-kidney, so we think that the changes of the animal model are related to the deficiency of the kidney in traditional Chinese medicine.
10.A clinical study of Gefitinib retreatment beyond progression in non-small cell lung cancer patients with rare EGFR mutations
Honghao MU ; Yun QING ; Qi FEI ; Dan QIU ; Jian FENG ; Lingli TU ; Lan SUN
Chongqing Medicine 2017;46(15):2072-2074
Objective To evaluate the effectiveness and safety of gefitinib retreatment beyond progression(GRBP)in non-small cell lung cancer(NSCLC)patients with rare EGFR mutations.Methods We retrospectively analyzed six rare-EGFR-mutation NSCLC patients from Jan 2011 to Dec 2015.Those patients had previous disease control and then disease progression according to Response Evaluation Criteria in Solid Tumors version 1.1(RECIST v1.1)after taking oral gefitinib 250 mg once a day.After that,continuing gefitinib was decided by clinicians′ experience at the same treatment option.The primary endpoints were response rate(RR),overall survival(OS),the first and second progression-free survival(PFS-1 and PFS-2).Safety was assessed according to the NCI-CTCAE version 4.0.Results After initial treatment of gefitinib,4 patients achieved partial response(PR)and 2 patients showed stable disease(SD),with RR being 66.7%.The median PFS-1 and PFS-2 were 10 months(95%CI 6.6-13.4)and 9 months(95%CI 6.9-11.1),respectively.The median OS time was 28 months(95%CI 10.4-45.6).The most common treatment-related adverse events were fatigue,diarrhea,rash,itching and elevated transaminases.Conclusion In our study,gefitinib retreatment beyond disease progression is effective with a manageable tolerability profile.